IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ wenta-2019-2 in vitro In vitro elisa enzyme linked immunosorbent assay ELISA ELISA elisa antibody detection identification by antibody antibody detection Wenta T., Rychlowski M., Jurewicz E., Jarzab M., Zurawa-Janicka D., Filipek A., Lipinska B., Wenta T., Rychlowski M., Jurewicz E., Jarzab M., Zurawa-Janicka D., Filipek A., Lipinska B. barbara.lipinska@biol.ug.edu.pl The FEBS journal 2019 Only protein-protein interactions imex curation imex curation wenta-2019-1 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Wenta T., Rychlowski M., Jurewicz E., Jarzab M., Zurawa-Janicka D., Filipek A., Lipinska B., Wenta T., Rychlowski M., Jurewicz E., Jarzab M., Zurawa-Janicka D., Filipek A., Lipinska B. barbara.lipinska@biol.ug.edu.pl The FEBS journal 2019 Only protein-protein interactions imex curation imex curation wenta-2019-3 in vitro In vitro protease assay protease assay experimental particp experimental participant identification experimental particp Wenta T., Rychlowski M., Jurewicz E., Jarzab M., Zurawa-Janicka D., Filipek A., Lipinska B., Wenta T., Rychlowski M., Jurewicz E., Jarzab M., Zurawa-Janicka D., Filipek A., Lipinska B. barbara.lipinska@biol.ug.edu.pl The FEBS journal 2019 Only protein-protein interactions imex curation imex curation wenta-2019-4 human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Wenta T., Rychlowski M., Jurewicz E., Jarzab M., Zurawa-Janicka D., Filipek A., Lipinska B., Wenta T., Rychlowski M., Jurewicz E., Jarzab M., Zurawa-Janicka D., Filipek A., Lipinska B. barbara.lipinska@biol.ug.edu.pl The FEBS journal 2019 Only protein-protein interactions imex curation imex curation wenta-2019-5 human-skbr3 Homo sapiens SKBR3 skbr3 SKBR3 Species: human; Tissue: breast; Tumor: adenocarcinoma; Derived from: metastatic site: pleural effusion anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wenta T., Rychlowski M., Jurewicz E., Jarzab M., Zurawa-Janicka D., Filipek A., Lipinska B., Wenta T., Rychlowski M., Jurewicz E., Jarzab M., Zurawa-Janicka D., Filipek A., Lipinska B. barbara.lipinska@biol.ug.edu.pl The FEBS journal 2019 Only protein-protein interactions imex curation imex curation wenta-2019-8 human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Wenta T., Rychlowski M., Jurewicz E., Jarzab M., Zurawa-Janicka D., Filipek A., Lipinska B., Wenta T., Rychlowski M., Jurewicz E., Jarzab M., Zurawa-Janicka D., Filipek A., Lipinska B. barbara.lipinska@biol.ug.edu.pl The FEBS journal 2019 Only protein-protein interactions imex curation imex curation wenta-2019-6 human-skbr3 Homo sapiens SKBR3 skbr3 SKBR3 Species: human; Tissue: breast; Tumor: adenocarcinoma; Derived from: metastatic site: pleural effusion confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Wenta T., Rychlowski M., Jurewicz E., Jarzab M., Zurawa-Janicka D., Filipek A., Lipinska B., Wenta T., Rychlowski M., Jurewicz E., Jarzab M., Zurawa-Janicka D., Filipek A., Lipinska B. barbara.lipinska@biol.ug.edu.pl The FEBS journal 2019 Only protein-protein interactions imex curation imex curation wenta-2019-7 human-hcc827 Homo sapiens hcc827 Non-small cell lung adenocarcinoma cell line HCC-827 confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Wenta T., Rychlowski M., Jurewicz E., Jarzab M., Zurawa-Janicka D., Filipek A., Lipinska B., Wenta T., Rychlowski M., Jurewicz E., Jarzab M., Zurawa-Janicka D., Filipek A., Lipinska B. barbara.lipinska@biol.ug.edu.pl The FEBS journal 2019 Only protein-protein interactions imex curation imex curation wenta-2019-10 human-skbr3 Homo sapiens SKBR3 skbr3 SKBR3 Species: human; Tissue: breast; Tumor: adenocarcinoma; Derived from: metastatic site: pleural effusion pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Wenta T., Rychlowski M., Jurewicz E., Jarzab M., Zurawa-Janicka D., Filipek A., Lipinska B., Wenta T., Rychlowski M., Jurewicz E., Jarzab M., Zurawa-Janicka D., Filipek A., Lipinska B. barbara.lipinska@biol.ug.edu.pl The FEBS journal 2019 Only protein-protein interactions imex curation imex curation wenta-2019-11 human-hcc827 Homo sapiens hcc827 Non-small cell lung adenocarcinoma cell line HCC-827 pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Wenta T., Rychlowski M., Jurewicz E., Jarzab M., Zurawa-Janicka D., Filipek A., Lipinska B., Wenta T., Rychlowski M., Jurewicz E., Jarzab M., Zurawa-Janicka D., Filipek A., Lipinska B. barbara.lipinska@biol.ug.edu.pl The FEBS journal 2019 Only protein-protein interactions imex curation imex curation xiap_human E3 ubiquitin-protein ligase XIAP Inhibitor of apoptosis protein 3 X-linked inhibitor of apoptosis protein IAP-like protein XIAP API3 IAP3 BIRC4 Baculoviral IAP repeat-containing protein 4 RING-type E3 ubiquitin transferase XIAP protein protein human Homo sapiens Human MTFNSFEGSKTCVPADINKEEEFVEEFNRLKTFANFPSGSPVSASTLARAGFLYTGEGDTVRCFSCHAAVDRWQYGDSAVGRHRKVSPNCRFINGFYLENSATQSTNSGIQNGQYKVENYLGSRDHFALDRPSETHADYLLRTGQVVDISDTIYPRNPAMYSEEARLKSFQNWPDYAHLTPRELASAGLYYTGIGDQVQCFCCGGKLKNWEPCDRAWSEHRRHFPNCFFVLGRNLNIRSESDAVSSDRNFPNSTNLPRNPSMADYEARIFTFGTWIYSVNKEQLARAGFYALGEGDKVKCFHCGGGLTDWKPSEDPWEQHAKWYPGCKYLLEQKGQEYINNIHLTHSLEECLVRTTEKTPSLTRRIDDTIFQNPMVQEAIRMGFSFKDIKKIMEEKIQISGSNYKSLEVLVADLVNAQKDSMQDESSQTSLQKEISTEEQLRRLQEEKLCKICMDRNIAIVFVPCGHLVTCKQCAEAVDKCPMCYTVITFKQKIFMS p83110-1 Serine protease HTRA3 HTRA3 PRSP High-temperature requirement factor A3 Pregnancy-related serine protease Long pL protein protein human Homo sapiens Human MQARALLLAALAALALAREPPAAPCPARCDVSRCPSPRCPGGYVPDLCNCCLVCAASEGEPCGGPLDSPCGESLECVRGLCRCRWSHAVCGTDGHTYANVCALQAASRRALQLSGTPVRQLQKGACPLGLHQLSSPRYKFNFIADVVEKIAPAVVHIELFLRHPLFGRNVPLSSGSGFIMSEAGLIITNAHVVSSNSAAPGRQQLKVQLQNGDSYEATIKDIDKKSDIATIKIHPKKKLPVLLLGHSADLRPGEFVVAIGSPFALQNTVTTGIVSTAQREGRELGLRDSDMDYIQTDAIINYGNSGGPLVNLDGEVIGINTLKVTAGISFAIPSDRITRFLTEFQDKQIKDWKKRFIGIRMRTITPSLVDELKASNPDFPEVSSGIYVQEVAPNSPSQRGGIQDGDIIVKVNGRPLVDSSELQEAVLTESPLLLEVRRGNDDLLFSIAPEVVM p83110-2 Serine protease HTRA3 HTRA3 PRSP High-temperature requirement factor A3 Pregnancy-related serine protease Short pS protein protein human Homo sapiens Human MQARALLLAALAALALAREPPAAPCPARCDVSRCPSPRCPGGYVPDLCNCCLVCAASEGEPCGGPLDSPCGESLECVRGLCRCRWSHAVCGTDGHTYANVCALQAASRRALQLSGTPVRQLQKGACPLGLHQLSSPRYKFNFIADVVEKIAPAVVHIELFLRHPLFGRNVPLSSGSGFIMSEAGLIITNAHVVSSNSAAPGRQQLKVQLQNGDSYEATIKDIDKKSDIATIKIHPKKKLPVLLLGHSADLRPGEFVVAIGSPFALQNTVTTGIVSTAQREGRELGLRDSDMDYIQTDAIINYGNSGGPLVNLDGEVIGINTLKVTAGISFAIPSDRITRFLTEFQDKQIKAPSLAVH htra3_human Serine protease HTRA3 HTRA3 PRSP High-temperature requirement factor A3 Pregnancy-related serine protease protein protein human Homo sapiens Human MQARALLLAALAALALAREPPAAPCPARCDVSRCPSPRCPGGYVPDLCNCCLVCAASEGEPCGGPLDSPCGESLECVRGLCRCRWSHAVCGTDGHTYANVCALQAASRRALQLSGTPVRQLQKGACPLGLHQLSSPRYKFNFIADVVEKIAPAVVHIELFLRHPLFGRNVPLSSGSGFIMSEAGLIITNAHVVSSNSAAPGRQQLKVQLQNGDSYEATIKDIDKKSDIATIKIHPKKKLPVLLLGHSADLRPGEFVVAIGSPFALQNTVTTGIVSTAQREGRELGLRDSDMDYIQTDAIINYGNSGGPLVNLDGEVIGINTLKVTAGISFAIPSDRITRFLTEFQDKQIKDWKKRFIGIRMRTITPSLVDELKASNPDFPEVSSGIYVQEVAPNSPSQRGGIQDGDIIVKVNGRPLVDSSELQEAVLTESPLLLEVRRGNDDLLFSIAPEVVM xiap-htra3-1 3672310 N/A 3672320 unspecified role unspecified role bait bait purified purified C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) N/A 3672321 unspecified role unspecified role prey prey purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 2A xiap-htra3-2 3672310 N/A 3672320 unspecified role unspecified role bait bait purified purified C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) N/A 3672322 unspecified role unspecified role prey prey purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 2B xiap-htra3-3 3672310 N/A 3672320 unspecified role unspecified role prey prey purified purified C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) N/A 3672322 unspecified role unspecified role bait bait purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false P83110-2:p.Ser305Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 2C htra3-xiap-4 3672310 N/A 3672320 unspecified role unspecified role prey prey purified purified C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) N/A 3672321 unspecified role unspecified role bait bait purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false P83110-1:p.Ser305Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 2C htra3-xiap-1 3672311 N/A 3672320 unspecified role unspecified role prey prey cell lysate cell lysate human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma N/A 3672321 unspecified role unspecified role bait bait purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P83110-1:p.Ser305Ala mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false 1A htra3-xiap-2 3672311 N/A 3672320 unspecified role unspecified role prey prey cell lysate cell lysate human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma N/A 3672322 unspecified role unspecified role bait bait purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P83110-2:p.Ser305Ala mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false 1A htra3-xiap-3 3672311 N/A 3672320 unspecified role unspecified role bait bait purified purified C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) N/A 3672323 unspecified role unspecified role prey prey cell lysate cell lysate human-skbr3 Homo sapiens SKBR3 skbr3 SKBR3 Species: human; Tissue: breast; Tumor: adenocarcinoma; Derived from: metastatic site: pleural effusion physical association physical association false false false 1B htra3-xiap-5 3672312 N/A 3672320 predetermined predetermined participant predetermined western blot western blot Immuno blot Immuno blot enzyme target enzyme target substrate substrate neutral component neutral component purified purified C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) N/A 3672321 predetermined predetermined participant predetermined enzyme enzyme neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) protein cleavage protein cleavage false false false 3D htra3-xiap-6 3672312 N/A 3672320 predetermined predetermined participant predetermined western blot western blot Immuno blot Immuno blot enzyme target enzyme target substrate substrate neutral component neutral component purified purified C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) N/A 3672322 predetermined predetermined participant predetermined enzyme enzyme neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) protein cleavage protein cleavage false false false 3D htra3-xiap-7 3672313 N/A 3672320 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3672321 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false P83110-1:p.Ser305Ala mutation mutation certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 4A htra3-xiap-8 3672313 N/A 3672320 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3672322 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed c-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P83110-2:p.Ser305Ala mutation mutation certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 4A htra3-xiap-9 3672314 N/A 3672320 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3672323 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false 4B htra3-xiap-10 3672314 N/A 3672320 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3672323 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false 4C htra3-xiap-15 3672315 N/A 3672320 unspecified role unspecified role neutral component neutral component cell lysate cell lysate N/A 3672321 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false proximity proximity close-contact colocalization neighbourhood interaction false false false 6 htra3-xiap-16 3672315 N/A 3672320 unspecified role unspecified role neutral component neutral component cell lysate cell lysate N/A 3672322 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false proximity proximity close-contact colocalization neighbourhood interaction false false false 6 htra3-xiap-11 3672316 N/A 3672320 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3672323 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level colocalization colocalization false false false 5A htra3-xiap-12 3672317 N/A 3672320 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3672323 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level colocalization colocalization false false false 5A htra3-xiap-13 3672318 N/A 3672320 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3672323 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level proximity proximity close-contact colocalization neighbourhood interaction false false false 5B htra3-xiap-14 3672319 N/A 3672320 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3672323 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level proximity proximity close-contact colocalization neighbourhood interaction false false false 5B