MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint gerber-2019-1 crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. bn-page blue native page bn-page sequence tag sequence tag identification sequence tag Gerber H., Mosser S., Boury-Jamot B., Stumpe M., Piersigilli A., Goepfert C., Dengjel J., Albrecht U., Magara F., Fraering PC. Alzheimers - Interactions investigated in the context of Alzheimers disease fraeringpatrick@hotmail.com Acta neuropathologica communications 2019 Only protein-protein interactions imex curation imex curation g3h2e5_crigr Olfactory receptor I79_004355 protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster MSKGRENETGVSEFLLLGITNDSGQQQILFWAFLCMYLVTVAGNTLILLAISSDPRLHTPMYFFLANLSFVDICFTTNLIPRLLASHVAGTRTISYTHCLTQMYFLISFANVDTFLLAAMALDRFVAICTIITPQLCVGLAAIVWVCSGLISLLHTLLMSRLNFCSLLPEISHFYCDAYLIMKSACSDTRVNQRVFLGAVVLFVAPCILIVVSYVRITIAVFRIPSAKGRYKAFSTCSSHLSVVTLFYGTVLGIYIRPPDSFSTQDTVATIMYTVVTPMLNPFIYSLRNKDMKETMTRLLNKGLKSS argi-1_crigr_protein Arginase-1 protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster g3hyq1_crigr I79_016192 protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster MEKASLIQKAKLAEQAERYEDMAAFMKGAVEKGEELSCEERNLLSVAYKNVVGGQRAAWRVLSSIEQKSNEEGSEEKGPEVKEYREKVETELRGVCDTVLGLLDSHLIKEAGEAGSRVFYLKMKGDYYRYLAEVATGDDKKRIIDSARSAYQEAMDISKKEMPPTNPIRLGLALNFSVFHYEIANSPEEAISLAKTTFDEAMADLHTLNEDSYKDSTLIMQLLRDNLTLWTADSAGEEGGEAPEEPQS spb3_crigr_protein Serpin B3 protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster similar to P29508 clus_crigr_protein Clusterin protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster similar to P10909 g3hgq1_crigr I79_009791 protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster MPLVCSLYVHPLPLLIGDSLLRSSSRSLLEFNTTTDCQPSDSQHRTQTSITFLCGKTLGTPEFVTATDCVHYFEWRTTAACKKDIFKADKEVPCYAFDDKLKKHDLNPLIKLSGGYLVDDSDPETSLFINVCRDIDSLRDPSTQLRACPAGTAACLLKGDQAFDVGRPKEGLKLVSKDRLVLSYVKEEAEKPDFCNGHSPAVTITFVCPSERREGTIPKLTASSNCRYEVEWITEYACHRDYLESETCSLSSEQHDIAIDLSPLAQQGGSSYVSDGREYTFYINVCGNATVPLCNLKESAVCQVKKADSTQVKIAGRHQNQTLRYSDGDLTLIYSGGDECSSGFQRMSVINFECNKTAGNDGRGEPVFTGEVDCTYFFTWDTRYACVKEKEDLLCGVSDGKKRYDLSVLARHSESEKNWEAVDGDQAESEKKYFFINVCHRVLQEGKARSCPEDAAVCAVDKNGSKNLGKFVSSPTSEKGYIQLSYSDGDDCGSDKKITTNITLVCKPGDLESAPVLRTAGPDGCFYEFEWHTAAACVLSKTEGENCTVFDAQAGFSFDLSPLTKKNGAYKVGTEKYDFYINVCGPVSVDLCQPNSGACQVAKRQEEDNSTYNFRWYTSYACPEEPLECMVTDPSMMEQYDLSSLVKSEGRSGGNWYAMENSREHVTRRKYYINVCRPLNPVPGCDRYAAACQMRYENSEGSLAETVSISNLGVAKTGPVVEESGSLLLEYVNGSACTTSDGRLTTYSTRIHLVCGRGSLNTHPIFTYNWECVVSFLWNTEAACPIQTITDTDQACSIRDPNSGFVFNLSPLNRSQGYVVPGIGKNFVFNICGAMPECGTIAGNPAFGCEAGTQTELESLKPERPVGMEKSLQLSTEGFLTLTYKGSSPSDKGTAFIIRFICNDDIYPGTAKFLHQDIDSTRGIRNTFFEFETALACVPSRVDCQVTDPAGNEYDLSALSMAGKPWTAVDTSVDGKKRSFYLSVCTPLPYIPGCHGSAVGSCMVLPAEDRGLNLGVVQISPQADANGSLSILYVNGDKCGNQRFSTRIIFECAQTLGSPVFQFVKDCEYMFVWRTVEACPVVREEGDNCQVKDPRHGNFYDLKPLALSDTIITAGEYTYYFRVCGKLSSNVCHVRDGSKVVSSCQEKKGSQGFQKVAGLLTQKLTYENGLLKMNYTGGDTCHQVYQRSTTIYFYCDRSTQKPVFLRETTDCSYLFEWRTQYACPPFNVTECSTQDGAGNSIDLSSLSRYSDNWEAVTRTGATEHYLINICKSLSPRAGGGEPCPPEAAVCLLDGSKPLNLGKVRDGPQWTGGVTVLKYVDGDLCPDQIRKRSTIIRFTCSESQVSSRPLFISAVQDCEYTFSWPTPAACPVKNSTHDDCQVTNPSTGHLFDLSSLSGKAGVTVSYSEKGLVYMSICGENENCGAGVGACFGQTRISVGKASKRLSYVDQVLQLVYEEGSLCPSKSGLRYKSVISFVCRPEAGPTNRPMLISLDKQTCTLFFSWHTPLACEQVTECTVRNGSSIIDLSPLIHRTGGYEAYDESEDGTSDTTPDFYINICQPLNPMHGVPCPAGAAVCKVPVNGPPIDIGRVTGPPIFNPVANEVYLNFESSTPCLADKHMNYTSLIAFHCKRGVSMGTPKLIRTNDCDFVFEWETPIVCPDEVKTQGCSVTDEQLLYSFNLTSLSTSTFKARLPSIARGSD g3h902_crigr I79_006863 protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster MASVCRGRVVRVPSGSLVRVVGTELVIPCNVSDYDGPIEQNFDWSFSSSGSSFLELASTWEPGFPAQLYRERLQRGDILLRRTANDAVELHIKNVQPSDQGHYKCSTPSTDATVQGNYEDTVQVKVLADALVVGPSSRPPPGLSLREGEPFELRCITSTMSPLHTHLALRWELHRGPVHRSILALSHEGRFHPGPGYEQRYHSGDVRLDTAGSDAYRLSVARALSADQGSYRCVVSEWITEQGNWQEIQEKAVEVATVVIQPTALQLAVPRTVSVTEGKDLDLSCNITTDRVDDVRPEVTWYFKKTPDASLLASHMLARLDRDSLVHSSPHVALSHVDTRSYHLLVRDASKENSGYYLCLVALWAPGHNRSWHKVAEAMSAPSSVSVTWLEPEYQVYLNASKVPGFSDDPTELQCRVIDMKRVEAGVRLTVSWYYRMNRRIGDEVVASELLAVMDGDWTLRYGERSKQRAQSGEFIFSKEHTDTFSFRIQRTTEEDRGNYYCVVSAWTRQRNNSWVKSKDVFSKPVNIFWASEDSVLVVRARQPKPFFAAGNTFEMTCKVSSKNIKSPRYSVLITAEKPVGDLTSPNETKYIISLDQDSVVKLENWTDASRVDGVVLEKVQEDEFRYRMYQTQVSDAGLYRCMVTAWSPIGGSLWREAATSLSNPIEIDFQTSDDMAFDVSWFAVHSFGLDKDPVLLSSLDRKGVVTTGQRDWKSTLSLERVSVLEFLLQVHGSEDQDFGNYYCSVTPWVRSPTGSWQREAEIHSRPIFITVKMDVLNAFKYPLLIGVGLSTVIGLLSCLIGYCSSHWCCKKEVRETRRERRRLMSMEMD g3h7x9_crigr Nicastrin I79_006475 protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster MVLLEGKLFNRAVMEKLKGRTSRIAGLAVTLAKPNSTSSFSPSVQCPNDGFGIYSNSYGSEFAHCKQTLWNELGNGLAYEDFSFPIFLLEDENETKVIKQCYQDHNLAQNGSAPRFPLCAMQLFSHMHAVISTATCMRRSFIQSTFSINPEIVCDPLSDYNVWSMLKPINTSGALEPDDRVVVAATRLDSRSFFWNVAPGAESAVASYVTQLAAAEALHKASDVATLPRNVMFVFFQGETFDYIGSSRMVYDMENGKFPVRLENIDSFVELGQVALRTSLDLWMHTDPMSQKNESVKNQVEDLLVTLEKSGAGVPEVVLRRQNQSQALPPSSLQRFLRARNISGVVLADHSGSFHNRYYQSIYDTAENINVTYPERQSPEEDLNFVTDTAKALANVATVLARALYELAGGTNFSDSIQADPQTVTRLLYGFLVRANNSWFQSILRQDLRSYLDDGPLQHYIAVTSPTNTTYVVQYALANLTGKVTNLTREQCQDPSKVPNESKALYDYSWVQGPLNSNKTERLPRCVRSTVRLARALSPAFELSQWGSTEYSTWAESRWKDIQARIFLVASKELEFITLTVGFGILIFSLIVTYCINAKADVLFVAPREPGAVSY lamp1_crigr Lysosome-associated membrane glycoprotein 1 CD107 antigen-like family member A Lysosomal membrane glycoprotein A LAMP1 LGPA protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster MAAPGAPRSLLLLLLAGLAHGASALFVVKDSNGTACIMANFSASFFTIYETGHGSKNSTFELPSSAEVLNSNSSCGRENVSEPILTIAFGSGYLLTLNFTRNATRYSVQDMYFAYNLSDTQHFLNASNKGIHSVDSSTDIKADINKTYRCLSAIQVHMGNVTVTLSDATIQAYLLNSNFSKEETRCTQDGPSPTTVPPSPSPPLVPTNPTVIKYNVTGENGTCLLASMALQMNITYMKKDNMTVTRALNISPNDTASGSCSPHVVTLTVESKNSILDLKFGMNGSSSLFFLQEVRLNMTLPDANVSSLMASNQSLRALQATVGNSYKCNTEEHIFVTKEFSLNVFSVQVQAFKVESDRFGSVEECMQDGNNMLIPIAVGGALAGLVLIVLIAYLIGRKRSHAGYQTI mbrl_crigr_protein Membralin protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster similar to F1LPC5 fetua_crigr_protein Fetuin-A protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster similar to P02765 apmap_human Adipocyte plasma membrane-associated protein Protein BSCv APMAP C20orf3 UNQ1869/PRO4305 protein protein human Homo sapiens Human MSEADGLRQRRPLRPQVVTDDDGQAPEAKDGSSFSGRVFRVTFLMLAVSLTVPLLGAMMLLESPIDPQPLSFKEPPLLLGVLHPNTKLRQAERLFENQLVGPESIAHIGDVMFTGTADGRVVKLENGEIETIARFGSGPCKTRDDEPVCGRPLGIRAGPNGTLFVADAYKGLFEVNPWKREVKLLLSSETPIEGKNMSFVNDLTVTQDGRKIYFTDSSSKWQRRDYLLLVMEGTDDGRLLEYDTVTREVKVLLDQLRFPNGVQLSPAEDFVLVAETTMARIRRVYVSGLMKGGADLFVENMPGFPDNIRPSSSGGYWVGMSTIRPNPGFSMLDFLSERPWIKRMIFKLFSQETVMKFVPRYSLVLELSDSGAFRRSLHDPDGLVATYISEVHEHDGHLYLGSFRSPFLCRLSLQAV a0a061i3k8_crigr H671_7g18220 protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster MEGKWLLCLLLVLGTVAVQAHDGHDDDMIDIEDDLDDVIEEVEDSKSKSDSSTPPSPKVTYKAPVPTGEVYFADSFDRGSLSGWILSKAKKDDTDDEIAKYDGKWEVDEMKDTKLPGDKGLVLMSRAKHHAISAKLNKPFLFDTKPLIVQYEVNFQNGIECGGAYVKLLSKTSELNLDQFHDKTPYTIMFGPDKCGEDYKLHFIFRHKNPKTGVYEEKHAKRPDTDLKIYFTDKKTHLYTLILNPDNSFEILVDQYVVNSGNLLNDMTPAVNPSREIEDPEDQKPEDWDERPKIPDPDAVKPDDWDEDAPGKIPDEEATKPEGWLDDEPEYIPDPDADKPEDWDEDMDGEWEAPQIANPKCESAPGCGVWQRPLIDNPNYKGKWKPPMIDNPNYQGVWKPRKIPNPDFFEDLEPFKMTPFSAIGLELWSMTSDIFFDNFIISGDRRVVDDWANDGWGLKKAADGAAEPGVVGHMLEAAEERPWLWVVYILTVALPVFLVILFCCSGKKQSNAMEYKKTDAAQPDVKEDEGKEEEKNKGDEEEEEEKLEEKQKSDAEEDGGTGSQDEEDRKPTAEEDEILNRSPRNRKPRRESWDLWHLHDTVKRNLDSAASPQNGDQQNGYGDLFPGHKKTRREAPLGISVSANGLPPASPLGQPDKPPGGDALQSGGKHSLGLDPINKKCMADSSIHLNGSSNPNEPFPLSLSKELKQEPVDDLPCMIAGAGGSVSQSNLMPDLNLNEQEWKELIEELNRSVPDEDMKDLFTEDFEEKKDPEPPGSATQTPLAQDINIKTEFSPAAFEQEQLGSPQVRAGSAGQTFLGPSSAPVGTDSPSLGSSQTLFHTTGQPGADNPSPNLMPASAQAQNAQRALASVVLPSQGPGGASELSSAHQLQQIAAKQKREQMLQNPQQAASAPAPGQLSTWQQAGPSHSPLDVPYPMEKPASPPGYKQDFTNSKLLMMPSVNKSSPRPGGPYLQPSHSNLLSHQSPSNLNQSSVNNQGAVLDYGNTKPLSHYKADCGQGGPGSGQSKPALMAYLPQQLPHLSNEQNSLFLMKQKAANMPFRSLVPPGQEQNPSNVSVPAQTANVGTQPPSVSVASTHNSSPYLSSQQQAAVMKQHQLLLDQQKQREQQQLQQQQQQFLQRQHLLAEQEKQQFQRPSAAVPGMNNLGPSNSSCPRVFPQPGTLMSMGPGHAPVSSLPSSSGPQDRGVAQFTGSQSLPQSSLYGITSGLPQIVAQPPPQAASAHAHIPRQTSVGQNASAPAAYGQNSLGSAGLSQQHNKGTLPPGLTKSQVPRVSAAMGGQNASWQHQGMPNLSSQTPGNSSVSPFAAPSSFHMQQAHLKIPGQQFSQAMPSRPMAPMSSAGAAGSMLPPVSTQQRNSAPAPAPPQAAPQQGLPGLSPAGPELGAFSQSSTSQMNSRAGLHCTQAYPVRTMGQELPFAFSGQPGSSGLSSVAGHTDLIDSLLKNRTSEEWINELDDLLGSQ rtn4_crigr_protein Reticulon-4 protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster ahsg-1 3964224 N/A 3964225 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level partially purified partially purified crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. N/A 3964226 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level partially purified partially purified crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. N/A 3964227 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level partially purified partially purified crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. N/A 3964228 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level partially purified partially purified crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. N/A 3964229 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level partially purified partially purified crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. N/A 3964230 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level partially purified partially purified crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. N/A 3964231 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level partially purified partially purified crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. N/A 3964232 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level partially purified partially purified crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. N/A 3964233 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level partially purified partially purified crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. N/A 3964234 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level partially purified partially purified crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. N/A 3964235 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level partially purified partially purified crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. N/A 3964236 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed partially purified partially purified flag tag flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. N/A 3964237 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level partially purified partially purified crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. N/A 3964238 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level partially purified partially purified crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. association association false false false F3D SF4 We next designed a multistep purification procedure for the high-grade purification of APMAP and APMAP protein complexes. he Flag-tagged APMAP and APMAP protein complexes were affinity purified following a previously described M2 anti-Flag antibody immuno-affinity procedure [40]. As a last purification step, the proteins eluted from the M2 resin were separated by size exclusion chromatography (SEC) and the fractions enriched in APMAP (Fig. (Fig.3b)3b) were further loaded onto a Blue Native gel (BN-PAGE), which allows the detection of APMAP protein complexes in their native conformations.