IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ klose-2018-2 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Klose R., Adam MG., Weis EM., Moll I., Wüstehube-Lausch J., Tetzlaff F., Oka C., Ehrmann M., Fischer A. FRMPD2L1:- there are no database entries for a mouse protein corresponding to this murine gene. The human gene may be an unprocessed pseudogene. JAG1 plasmids were determined to be of Human origin by Blat of "Mutagenesis primers for the deletion of the Jag1-PDZ-binding site". a.fischer@dkfz.de mouse embryo (stage E13) Oncogene 2018 Only protein-protein interactions imex curation imex curation klose-2018-1 human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Klose R., Adam MG., Weis EM., Moll I., Wüstehube-Lausch J., Tetzlaff F., Oka C., Ehrmann M., Fischer A. JAG1 plasmids were determined to be of Human origin by Blat of "Mutagenesis primers for the deletion of the Jag1-PDZ-binding site". a.fischer@dkfz.de Oncogene 2018 Only protein-protein interactions imex curation imex curation htra1_mouse Serine protease HTRA1 Htra1 Htra Prss11 High-temperature requirement A serine peptidase 1 Serine protease 11 protein protein mouse Mus musculus Mouse MQSLRTTLLSLLLLLLAAPSLALPSGTGRSAPAATVCPEHCDPTRCAPPPTDCEGGRVRDACGCCEVCGALEGAACGLQEGPCGEGLQCVVPFGVPASATVRRRAQAGLCVCASSEPVCGSDAKTYTNLCQLRAASRRSEKLRQPPVIVLQRGACGQGQEDPNSLRHKYNFIADVVEKIAPAVVHIELYRKLPFSKREVPVASGSGFIVSEDGLIVTNAHVVTNKNRVKVELKNGATYEAKIKDVDEKADIALIKIDHQGKLPVLLLGRSSELRPGEFVVAIGSPFSLQNTVTTGIVSTTQRGGKELGLRNSDMDYIQTDAIINYGNSGGPLVNLDGEVIGINTLKVTAGISFAIPSDKIKKFLTESHDRQAKGKAVTKKKYIGIRMMSLTSSKAKELKDRHRDFPDVLSGAYIIEVIPDTPAEAGGLKENDVIISINGQSVVTANDVSDVIKKENTLNMVVRRGNEDIVITVIPEEIDP jag1_human Protein jagged-1 JAG1 JAGL1 protein protein human Homo sapiens Human MRSPRTRGRSGRPLSLLLALLCALRAKVCGASGQFELEILSMQNVNGELQNGNCCGGARNPGDRKCTRDECDTYFKVCLKEYQSRVTAGGPCSFGSGSTPVIGGNTFNLKASRGNDRNRIVLPFSFAWPRSYTLLVEAWDSSNDTVQPDSIIEKASHSGMINPSRQWQTLKQNTGVAHFEYQIRVTCDDYYYGFGCNKFCRPRDDFFGHYACDQNGNKTCMEGWMGPECNRAICRQGCSPKHGSCKLPGDCRCQYGWQGLYCDKCIPHPGCVHGICNEPWQCLCETNWGGQLCDKDLNYCGTHQPCLNGGTCSNTGPDKYQCSCPEGYSGPNCEIAEHACLSDPCHNRGSCKETSLGFECECSPGWTGPTCSTNIDDCSPNNCSHGGTCQDLVNGFKCVCPPQWTGKTCQLDANECEAKPCVNAKSCKNLIASYYCDCLPGWMGQNCDININDCLGQCQNDASCRDLVNGYRCICPPGYAGDHCERDIDECASNPCLNGGHCQNEINRFQCLCPTGFSGNLCQLDIDYCEPNPCQNGAQCYNRASDYFCKCPEDYEGKNCSHLKDHCRTTPCEVIDSCTVAMASNDTPEGVRYISSNVCGPHGKCKSQSGGKFTCDCNKGFTGTYCHENINDCESNPCRNGGTCIDGVNSYKCICSDGWEGAYCETNINDCSQNPCHNGGTCRDLVNDFYCDCKNGWKGKTCHSRDSQCDEATCNNGGTCYDEGDAFKCMCPGGWEGTTCNIARNSSCLPNPCHNGGTCVVNGESFTCVCKEGWEGPICAQNTNDCSPHPCYNSGTCVDGDNWYRCECAPGFAGPDCRININECQSSPCAFGATCVDEINGYRCVCPPGHSGAKCQEVSGRPCITMGSVIPDGAKWDDDCNTCQCLNGRIACSKVWCGPRPCLLHKGHSECPSGQSCIPILDDQCFVHPCTGVGECRSSSLQPVKTKCTSDSYYQDNCANITFTFNKEMMSPGLTTEHICSELRNLNILKNVSAEYSIYIACEPSPSANNEIHVAISAEDIRDDGNPIKEITDKIIDLVSKRDGNSSLIAAVAEVRVQRRPLKNRTDFLVPLLSSVLTVAWICCLVTAFYWCLRKRRKPGSHTHSASEDNTTNNVREQLNQIKNPIEKHGANTVPIKDYENKNSKMSKIRTHNSEVEEDDMDKHQQKARFAKQPAYTLVDREEKPPNGTPTKHPNWTNKQDNRDLESAQSLNRMEYIV a0a140li67_mouse Frmpd2 Gm626 protein protein mouse Mus musculus Mouse MRPLAKDTGMSVASVTLASALQVRGEALSEEEIWSLLSLAAERLLEDLRNDSSDYVVCPWSLLLSAAGGLSFQDHVSHIEAAPFKAPELLQGSNEEGQPDASQMHVYSLGMTLYWSAGFRVPPNQPLQLQEPVHSLLLAMCEDQPRRRQPLQAVLEACRIHQEEVAVYPAPASLHISRLVGLVLGTISEVERRVVEENACEQRNRSCSLRSRLHQADGESPGAPASDALQPRRVSERSAETQSSLERCTAHSRNSFVNSTFAGAGPCDCEESPWYGSEPMLLAEAESSQPATSSPRNFLQRKGKFSRPEFILLAGEAPVTLHLPGSIVTKKGKSYLALRDLCVVLLNGRCLEVKCDMESTAGAVFNAVMSFANLEETTYFGLAYVEGEEFFFLDKDTRLCKVAPEGWREQHPKGSVDTFTLFLRIRFFVGHYRLLRHSLTRHQFYLQLRKDILEERLYCNDETLLQLGVLALQAEFGSYPKEQVEGKAYFRIQDYIPARLIERMTAIRVQVEVSEMHRLSSAPWGEDAELEFLEIVQQLPEYGVLVHRVSPEKKRPEGKMALGVCTKGIIVYEVRGSRRIATSQFPWRETGMISTHRKKLTITSSTTGKKYTFVTNSSKTCKYLLGLCSSQHWFNAQTGSKHPSHRFTGQDKFAQIASLSSAHQTQANPITWIQKLSCSENELCVPRLQDATGGQLGTSMENKQGFKEPGKERIGSSPYTGGEQLHSIGLNQKPAQTVFGTAGHSMCAGSNCLGRRTQICCFDSVSGSQNSKRKGLFGEPNQDIVCVMLKRDPVHGFGFVINEGEDAEQTRSGIFISSLIPGGPAERAKKIKPGGKILALNHISLEGFTFSMAVRMIQNSPDDMELIISQPKGVCGTIHSEEKNSTASSGMFCTDILSNRCQGRQSPHIHDQDRSVRGTEMAQGAGSCPPSPLQTNTGEIYFVELVKEDGTLGFSVTGGINTSVPHGGIYVKSIIPGGPAAKEGQILQGDRLLQVDGVSLCGLTHKQAVQCLKGPGQVARLVLERRGPRAAPQCPSADDRMGDVHMAVSLVTARPGRPASSVSVTDGPKFEVKLKKNSRGLGFSFVQMERGNCIHTKSDLVRIKRLFPGQPAEEHGAIAAGDIILAVNGKPIEGLAFQEVLHLLRGAPEEVTLLLCRPPPGILPEMEPGWQTPELSGDQRLTMATCAGSEQSPSLDQEDNWRDSTSLDAGEGLSPGPESSYKDVRQVKGDREKERPWAKSWMHPMESHPHVCKLHPEPETPALATSLEKDMRQNCYSVCDIRRLGSLELDRDGADGATCFLPESSSLTVDYEEYLTLTSASAGQLPCEECLEADSETIPLPQFCSLGALLKSSLPEESQGSESDWEDLDEPVDRDEVLRWTRSARQPLAAEP mpp2_mouse MAGUK p55 subfamily member 2 Protein MPP2 Discs large homolog 2 Mpp2 Dlgh2 protein protein mouse Mus musculus Mouse MPVAATNSESAMQQVLDNLGSLPNATGAAELDLIFLRGIMESPIVRSLAKAHERLEETKLEAVRDNNLELVQEILRDLAELAEQSSTAAELARILQEPHFQSLLETHDSVASKTYETPPPSPGLDPTFSNQPVPPDAVRMVGIRKTAGEHLGVTFRVEGGELVIARILHGGMVAQQGLLHVGDIIKEVNGQPVGSDPRALQELLRSASGSVILKILPSYQEPHLPRQVFVKCHFDYDPARDSLSPCKEAGLRFNAGDLLQIVNQDDANWWQACHVEGGSAGLIPSQLLEEKRKAFVKRDLELTPTSGTLCGSLSGKKKKRMMYLTTKNAEFDRHELLIYEEVARMPPFRRKTLVLIGAQGVGRRSLKNKLILWDPDRYGTTVPYTSRRPKDSEREGQGYSFVSRGEMEADIRAGRYLEHGEYEGNLYGTRIDSIRGVVASGKVCVLDVNPQAVKVLRTAEFVPYVVFIEAPDYETLRAMNRAALESGVSTKQLTEADLRRTVEESSRIQRGYGHYFDLSLVNSNLERTFRELQTAMEKLRTEPQWVPVSWVY sdcb1_mouse Syntenin-1 Syndecan-binding protein 1 Scaffold protein Pbp1 Sdcbp protein protein mouse Mus musculus Mouse MSLYPSLEDLKVDKVIQAQTAYSANPASQAFVLVDASAALPPDGNLYPKLYPELSQYMGLSLNEAEICESMPMVSGAPAQGQLVARPSSVNYMVAPVTGNDAGIRRAEIKQGIREVILCKDQDGKIGLRLKSIDNGIFVQLVQANSPASLVGLRFGDQVLQINGENCAGWSSDKAHKVLKQAFGEKITMTIRDRPFERTVTMHKDSSGHVGFIFKSGKITSIVKDSSAARNGLLTDHHICEINGQNVIGLKDAQIADILSTAGTVVTITIMPTFIFEHIIKRMAPSIMKSLMDHTIPEV htra1_human Serine protease HTRA1 HTRA1 HTRA PRSS11 High-temperature requirement A serine peptidase 1 L56 Serine protease 11 protein protein human Homo sapiens Human MQIPRAALLPLLLLLLAAPASAQLSRAGRSAPLAAGCPDRCEPARCPPQPEHCEGGRARDACGCCEVCGAPEGAACGLQEGPCGEGLQCVVPFGVPASATVRRRAQAGLCVCASSEPVCGSDANTYANLCQLRAASRRSERLHRPPVIVLQRGACGQGQEDPNSLRHKYNFIADVVEKIAPAVVHIELFRKLPFSKREVPVASGSGFIVSEDGLIVTNAHVVTNKHRVKVELKNGATYEAKIKDVDEKADIALIKIDHQGKLPVLLLGRSSELRPGEFVVAIGSPFSLQNTVTTGIVSTTQRGGKELGLRNSDMDYIQTDAIINYGNSGGPLVNLDGEVIGINTLKVTAGISFAIPSDKIKKFLTESHDRQAKGKAITKKKYIGIRMMSLTSSKAKELKDRHRDFPDVISGAYIIEVIPDTPAEAGGLKENDVIISINGQSVVSANDVSDVIKRESTLNMVVRRGNEDIMITVIPEEIDP jag1-htra1-2 3672814 N/A 3672816 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 3672817 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed ICD sufficient to bind sufficient binding region sufficient to bind range range range range false physical association physical association false false false Table 1 jag1-htra1-1 3672814 N/A 3672818 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 3672817 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed ICD sufficient to bind sufficient binding region sufficient to bind range range range range false physical association physical association false false false Table 1 jag1-mpp2-1 3672814 N/A 3672819 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 3672817 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed ICD sufficient to bind sufficient binding region sufficient to bind range range range range false physical association physical association false false false Table 1 jag1-sdcbp-1 3672814 N/A 3672820 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 3672817 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed ICD sufficient to bind sufficient binding region sufficient to bind range range range range false physical association physical association false false false Table 1 htra1-jag1-1 3672815 N/A 3672817 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed PDZ_binding_motif required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false N/A 3672821 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 3G