IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ de-2017-2 chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western De Filippo E., Schiedel AC., Manga P. Rare diseases - Interactions investigated in the context of Rare genetic disease schiedel@uni-bonn.de,Prashiela.Manga@nyumc.org The Journal of investigative dermatology 2017 Only protein-protein interactions imex curation imex curation Dr. P Sergouniotis PhD University of Manchester, UK de-2017-3 mouse-melan-a Mouse melan-a - immortalised melanocyte cell line melan-a Mouse melan-a - immortalised melanocyte cell line anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western De Filippo E., Schiedel AC., Manga P. Rare diseases - Interactions investigated in the context of Rare genetic disease schiedel@uni-bonn.de,Prashiela.Manga@nyumc.org The Journal of investigative dermatology 2017 Only protein-protein interactions imex curation imex curation Dr. P Sergouniotis PhD University of Manchester, UK de-2017-4 chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 confocal microscopy confocal microscopy immunostaining immunostaining De Filippo E., Schiedel AC., Manga P. Rare diseases - Interactions investigated in the context of Rare genetic disease schiedel@uni-bonn.de,Prashiela.Manga@nyumc.org The Journal of investigative dermatology 2017 Only protein-protein interactions imex curation imex curation Dr. P Sergouniotis PhD University of Manchester, UK de-2017-5 mouse-melan-a Mouse melan-a - immortalised melanocyte cell line melan-a Mouse melan-a - immortalised melanocyte cell line confocal microscopy confocal microscopy immunostaining immunostaining De Filippo E., Schiedel AC., Manga P. Rare diseases - Interactions investigated in the context of Rare genetic disease schiedel@uni-bonn.de,Prashiela.Manga@nyumc.org The Journal of investigative dermatology 2017 Only protein-protein interactions imex curation imex curation Dr. P Sergouniotis PhD University of Manchester, UK de-2017-6 chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret tag fluorescence tag visualisation by fluorescence tag fluorescence De Filippo E., Schiedel AC., Manga P. Rare diseases - Interactions investigated in the context of Rare genetic disease schiedel@uni-bonn.de,Prashiela.Manga@nyumc.org The Journal of investigative dermatology 2017 Only protein-protein interactions imex curation imex curation Dr. P Sergouniotis PhD University of Manchester, UK tyro_human Tyrosinase LB24-AB Monophenol monooxygenase SK29-AB Tumor rejection antigen AB TYR protein protein human Homo sapiens Human MLLAVLYCLLWSFQTSAGHFPRACVSSKNLMEKECCPPWSGDRSPCGQLSGRGSCQNILLSNAPLGPQFPFTGVDDRESWPSVFYNRTCQCSGNFMGFNCGNCKFGFWGPNCTERRLLVRRNIFDLSAPEKDKFFAYLTLAKHTISSDYVIPIGTYGQMKNGSTPMFNDINIYDLFVWMHYYVSMDALLGGSEIWRDIDFAHEAPAFLPWHRLFLLRWEQEIQKLTGDENFTIPYWDWRDAEKCDICTDEYMGGQHPTNPNLLSPASFFSSWQIVCSRLEEYNSHQSLCNGTPEGPLRRNPGNHDKSRTPRLPSSADVEFCLSLTQYESGSMDKAANFSFRNTLEGFASPLTGIADASQSSMHNALHIYMNGTMSQVQGSANDPIFLLHHAFVDSIFEQWLRRHRPLQEVYPEANAPIGHNRESYMVPFIPLYRNGDFFISSKDLGYDYSYLQDSDPDSFQDYIKSYLEQASRIWSWLLGAAMVGAVLTALLAGLVSLLCRHKRKQLPEEKQPLLMEKEDYHSLYQSHL gp143_human G-protein coupled receptor 143 GPR143 OA1 Ocular albinism type 1 protein protein protein human Homo sapiens Human MASPRLGTFCCPTRDAATQLVLSFQPRAFHALCLGSGGLRLALGLLQLLPGRRPAGPGSPATSPPASVRILRAAAACDLLGCLGMVIRSTVWLGFPNFVDSVSDMNHTEIWPAAFCVGSAMWIQLLYSACFWWLFCYAVDAYLVIRRSAGLSTILLYHIMAWGLATLLCVEGAAMLYYPSVSRCERGLDHAIPHYVTMYLPLLLVLVANPILFQKTVTAVASLLKGRQGIYTENERRMGAVIKIRFFKIMLVLIICWLSNIINESLLFYLEMQTDINGGSLKPVRTAAKTTWFIMGILNPAQGFLLSLAFYGWTGCSLGFQSPRKEIQWESLTTSAAEGAHPSPLMPHENPASGKVSQVGGQTSDEALSMLSEGSDASTIEIHTASESCNKNEGDPALPTHGDL tyro_mouse Tyrosinase Monophenol monooxygenase Albino locus protein Tyr protein protein mouse Mus musculus Mouse MFLAVLYCLLWSFQISDGHFPRACASSKNLLAKECCPPWMGDGSPCGQLSGRGSCQDILLSSAPSGPQFPFKGVDDRESWPSVFYNRTCQCSGNFMGFNCGNCKFGFGGPNCTEKRVLIRRNIFDLSVSEKNKFFSYLTLAKHTISSVYVIPTGTYGQMNNGSTPMFNDINIYDLFVWMHYYVSRDTLLGGSEIWRDIDFAHEAPGFLPWHRLFLLLWEQEIRELTGDENFTVPYWDWRDAENCDICTDEYLGGRHPENPNLLSPASFFSSWQIICSRSEEYNSHQVLCDGTPEGPLLRNPGNHDKAKTPRLPSSADVEFCLSLTQYESGSMDRTANFSFRNTLEGFASPLTGIADPSQSSMHNALHIFMNGTMSQVQGSANDPIFLLHHAFVDSIFEQWLRRHRPLLEVYPEANAPIGHNRDSYMVPFIPLYRNGDFFITSKDLGYDYSYLQESDPGFYRNYIEPYLEQASRIWPWLLGAALVGAVIAAALSGLSSRLCLQKKKKKKQPQEERQPLLMDKDDYHSLLYQSHL gpr143-tyr-1 3652242 N/A 3652247 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3652248 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell P51810:p.[Leu223_Leu224delinsAlaAla;Trp329_Glu330delinsAlaAla] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false fusion tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 2a, S2 gpr143-tyr-3 3652242 N/A 3652247 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3652248 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell fusion tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false P51810:p.[Leu223_Leu224delinsAlaAla;Trp329_Glu330delinsAlaAla] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false S3 gpr143-tyr-2 3652243 N/A 3652248 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false P51810:p.[Leu223_Leu224delinsAlaAla;Trp329_Glu330delinsAlaAla] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false N/A 3652249 western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false 2b, S4 gpr143-tyr-4 3652244 N/A 3652247 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3652248 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell fusion tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false P51810:p.[Leu223_Leu224delinsAlaAla;Trp329_Glu330delinsAlaAla] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false colocalization colocalization false false false 3a gpr143-tyr-5 3652245 N/A 3652249 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell N/A 3652248 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell region eyfp enhanced yellow fluorescent protein tag eyfp undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false 3b gpr143-tyr-6 3652246 N/A 3652248 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed living cell living cell P51810:p.[Leu223_Leu224delinsAlaAla;Trp329_Glu330delinsAlaAla] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false c-terminal region eyfp enhanced yellow fluorescent protein tag eyfp c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 3652247 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed living cell living cell c-terminal region cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false proximity proximity close-contact colocalization neighbourhood interaction false false false 4, 5, S5, S6, S7, S8