IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ tripsianes-2014-1 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Tripsianes K., Friberg A., Barrandon C., Brooks M., van Tilbeurgh H., Seraphin B., Sattler M. sattler@helmholtz-muenchen.de The Journal of biological chemistry 2014 Only protein-protein interactions imex curation imex curation tripsianes-2014-2 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Tripsianes K., Friberg A., Barrandon C., Brooks M., van Tilbeurgh H., Seraphin B., Sattler M. sattler@helmholtz-muenchen.de The Journal of biological chemistry 2014 Only protein-protein interactions imex curation imex curation cwc26_yeast Pre-mRNA-splicing factor CWC26 YGL174W G1642 BUD13 SLC7 Complexed with CEF1 protein 26 Bud site selection protein 13 Synthetic lethal with CLF1 protein 7 CWC26 protein protein yeast Saccharomyces cerevisiae Baker's yeast MALHQYLSETYGPTKPKNKTKKKKKESKSDANSDKTSLIVKERLSTLQQEQEKSGVASFSKFDKQKSKNIWKNLETNELSHAITHPSASSITGNESKNDLKEIRAQEPLVTVADKSKTRKTIYRDAQGHKIQEDSKIDDSSFSRSKYEDEKAAEREQYLKNLNMGDVQKLGINVDAHDKKKNQTASSLTIEDPAITFTHDKERTVKTSLLGRKLYDKPAPENRFAIMPGSRWDGVHRSNGFEEKWFAKQNEINEKKVQSYTLQEDY 1584B390121325CD ist3_yeast U2 snRNP component IST3 IST3 SNU17 YIR005W YIB5W U2 snRNP protein SNU17 Increased sodium tolerance protein 3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MNKIQQINDKELQSGILSPHQSWHNEYKDNAYIYIGNLNRELTEGDILTVFSEYGVPVDVILSRDENTGESQGFAYLKYEDQRSTILAVDNLNGFKIGGRALKIDHTFYRPKRSLQKYYEAVKEELDRDIVSKNNAEKLILAKKDQPN 9D51C3043AB823D2 bud13-ist3-2 3711493 N/A 3711495 unspecified role unspecified role neutral component neutral component purified purified Bud13p sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecobd Escherichia coli (strain B / BL21-DE3) Stoichiometry: 1.0 N/A 3711496 unspecified role unspecified role neutral component neutral component purified purified Snu17p RRM domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecobd Escherichia coli (strain B / BL21-DE3) Stoichiometry: 1.0 3711501 3711499 direct interaction direct interaction false false false Fig 6, Table 1, Fig 3, Fig 4 bud13-ist3-4 3711494 N/A 3711495 unspecified role unspecified role neutral component neutral component purified purified Bud13p - 22-mer sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Bud13p - 14-mer sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Bud13p - 22-mer required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false Bud13p - 66-mer sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecobd Escherichia coli (strain B / BL21-DE3) Stoichiometry: 1.0 N/A 3711496 unspecified role unspecified role neutral component neutral component purified purified Snu17p (residues 1–113) sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecobd Escherichia coli (strain B / BL21-DE3) Stoichiometry: 1.0 3711509 3711507 direct interaction direct interaction false false false Fig. 3