MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint dodd-2014-2 human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. pull down pull down anti tag western anti tag western blot anti tag western Dodd DA., Worth RG., Rosen MK., Grinstein S., van Oers NS., Hansen EJ. Hansen@UTSouthwestern.edu mBio 2014 Only protein-protein interactions imex curation imex curation dodd-2014-3 in vitro In vitro protein kinase assay protein kinase assay antibody detection identification by antibody antibody detection Dodd DA., Worth RG., Rosen MK., Grinstein S., van Oers NS., Hansen EJ. Hansen@UTSouthwestern.edu mBio 2014 Only protein-protein interactions imex curation imex curation dodd-2014-1 human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. pull down pull down ms participant Identification by mass spectrometry ms participant Dodd DA., Worth RG., Rosen MK., Grinstein S., van Oers NS., Hansen EJ. Hansen@UTSouthwestern.edu mBio 2014 Only protein-protein interactions imex curation imex curation q7vle8_haedu lspA1 HD_1505 protein protein haedu Haemophilus ducreyi (strain 35000HP / ATCC 700724) MNNKRYKLIFSKVKNCLVPVAENIKSASGNSGSSSNSKIAEDQEEEPDSLACSLSPLSSSIHLGLHNHSPLKVFKGKSLSVVLLSLMPATPLLAQQNYAEALNGKVYVDSQHSSTRIYEQKTNDNSKDGIVVVEIANPEVDGVSDNRFKEFNIPNSAVFNNSRTESTSQLVGKLHANIQLQKEAKLILNQVTGDHESNIQGALEVAGKKADLIIVNPNGITLNGVKTINTDRFVVSTSDIIPHRENGLLSVRNGKVTIDKGGVATNGLSHFEVVARNIDQKGKITVAKTENQKSVNPANITFAAGSLNYNLKTREATPISSGTSRTSDTPAISADSAGSMYGSNIKFVVTDKGAGVKHKGIIFSENDINIKMDGGNASLKELYAKKDIDILAKDIELTEKGQLQANNKIILNSTGKINLRNASEVSADNVNVKSENLALENASMSANSLDVIVTKIEVNRSSKVSAGTANIKASNITLDGSSVVANKITLNVTNNATLNNQSKLSAKDMELNVTHNITLNNTSKLSAQKANIKTENLTLNGEASLVAEKLDINAIDKITNNGTIAGLTANITTKALENRDNALILAHQNLNFTVNGSHYVNKGDIVSKDKAIVTFSNNSDFTSNGSKLVDAQNNLTVNVNNFNITQGSEIILHGNVTLNAKGNFTNSGNLTTMKELNISNIESFINAGNLTTGKNLEVHSNTTVKNDGKLVSIENLNISSKTDFTNNGTLLGLEALKIASGGNFTNASNGSLASNKSLDIYGNNFTNNGTIESVKSLNITNNYTFINNATIKSYGVLNITSQGNFTNDSNGTVMSHDLLNITSQANIINKNLLAGGQGLNLTAKGNITNDSNSTAIAVLHSNNDINLNANNKVYNIGEIYSQAGNISVEAKLLHNDVKLSGNITTTTKSGNATVKTNSIGGGLHDANSIRVGELTLNGKFADLDNQLKVALRGKIYAGSNLTFKAKEGEKEQKSTAQAKIINRGTINVKNKLEYGSNVDVENNMRSMQVNLYEKIFNGDNPITLTLKNGVTFAKDFSNRRRRASNDGEGTNKKTFDNVAHLIEEAFSGYSNGNDHRASDDGHVKSPYYLLVLAQAVNNTEGENYLKTALQHIFGPNWNDLTTTNNDTTINDKWNQLKLKWEKFKNNGENNHSINLNIYPADEGVEKAKIFAGVLRNGTNGVEDKVYQELNDKAKKEYEDKFAKKFQGRFKSRFQNGEFDWAGDWAKEGNESYGSKETEEKYNGIKKEHTVNIGKHEIKVPTVSFENLNNINHQQDKSDGIDKSIISELLAQPIYVAKADVPDVDPRVAQNDKAVDEDGLYRTRLSYINQNNYLGAKYFFNQLDTEDDKLKGIKRIGDNYFEHQLITRLIEKVADNHLTLKHGLHDIALVKKLIDSASIQAKDLNLKVGEALTKEQKDNLKEDIVWYVKTEVNGQEVLVPQVYLAKQTIEEVEKQRGVGTGQIRAGIIDVKVDDVRNTGTIAGYAVGLEAKNKLKNTGDILSQRLSKLVGKKGLESTGVTYVDETGATKVRKARIKSEGHIYLETDKDKNVDLTASELKGNTGQIKAKDLNLNDIYETSYKYKYEKLFGKNGGEIGDRVTQTSQAKSVGTDASFDHLHLSLEGDVNQTGSNLKANRTTGVVKGDFNTKAGKDLFHRQIDTVTSGTVYSASASGGGQSAGISLTDQGVETYTNKTATAGANADVTNFMKRTRETETSLTHRNSEFNALSGELYVMGKADIGGVDINRDVEVIKTPEEIAAEQKAAEEAKKAEVKENEASETAAKETEEAENDNVAEKDKTKPKFKKLTDEEIAAAFETKGEDFFAAYKAREEEDRKKGFTLSAEQIESTKARDEKETTYYELKVGVGAEAEAHSAAADAISNKARQIIDTQNGLKQDGTVALQEASDVLNLATGDLAGASAKLKFELSTIEKKSRGASDGRSILGGRLNLAARGGDITLNNVETTENSHLSLKARDNVNVNSGVTEQKDESNSQSLKVTAGASSGCGVMAGGCSAGVSAGVSGSYNESNTESTSHTNSLLRGKSLRVEAGKDFNLISSNVDVDHLHLDVKGDTNVVSKQDSYSRKERGVNYSVSAGVGVSTAGGARPNGSVGLGVSAENENSKIVKQQAGISAKRITGEINNLNLTGGYIENKGNPDELNVKGDITTHELKDEHHKDGGSFGGSVGVSETGVTQVNVNGGRVEQKHYEATQHSSISGINTKGKTVGNFKTDRSQSTEVHRDDTIAATNFNFELGDIAELAKKGKEKWDNRSAKTTSSSQDSAHDPRSRSVENGYSELPRFKTADNDAGVDSPRLIKGEAEQAQTLALTKAGNDVIPEVQSLTQKARPQSLVDESPYAEIPALTRPQVKSNIAESIEVPQFRTKVSDGDEGNYAEITFPTNKAAISSTQDVGDTPTPRALRLESESGYESAENLGLIPRGFKSSPKGEYEDISDAIEPQTRSRKLDEPEPIYGTINKSPEAIARANAKADEAIQALGYDPRIKPVVPEEAPPALPPRNLQTKAISDYDDVSYVPDFKVRKTDEPEPIYGTINKSPEAIARANAKADEAIQASGYDPRIKPVVPEDAPPALPPRTQSLIDSTEVPSYRSALANVKFDDASPWPQPSALRSKAFADEPSSETPKSRGKRGISEESLSSTVQPRSRKISEEDSSFERLPLRIIDNGSDYAEILPRNVKQTNEPATQAIRAPKALDNNDVIAERPSFKLRQLDDDVESVNGIYSSIKPKALIEEGTPITRQVKTVQEETPVTDLVNKRELVKEDRSLLDKVQDTFQPLKVRSKINDVRSSVEEYGGEVTFKYAQSKGEVYNEIVKHAETQNGVCEATCSHWIAKKVNDENIWTDLYKDGQKGRKGGLNKDAIESIEKLQTEFINAGTATQQFKLTNTWLEEQGVVPKQKYFGKLSRADEVAGTVSKNDVSALVKAILDTGNESSAVKKISINLEGGSHTVSASIEGQKVVFFDPNFGEITFKDKKSFEKWMKNAFWKKSGYAGKKDTKRFFNVVNYHKNSKRNKVIDVNQNHIQQLAGSEGFSPSLPTRPQLANAAGIKSNEMSSLFSWSKLKHLFSRESGKKAQVEGPEIKHLGGVVDKDAFYFPLDKIVTRRDAEGEIRVNMDNIKKAFNPRDKHYNSQEARSLRSLYNQDPSMSGTRFIIENQVIANPFSSADLQSYIQAQQSKLPELGRQARRALPELPTAANKGRGSRVEEQNIVTRPRVEDVYATVNKGAKHGEAQQPGSFYTKKLVDQVSHVPNTEPVYADLHFNRNGRVVRQTEPEVIYEKIRGQQVEVDDPSSLYAKVNRNRRLDNVEGFYPPEQLRTRSDKLAEQVSRVPTTEPVYADLRFKSAEDDYAPALPARPELGNAAGFRKAKVKGEESESTWSRLKHLFSRESGKTKVEEVENEYKSQTNGENSLEIKTIEHIPTRLKNLESPREAQSDLGENALIYGLQRGRQALISKANAADKEGKNAILADSYIGKLNLGFEFGELTKFAKQVKDGKVTEQDIQNIASFNDETAKLARRSEPKNRINDANVDDNQRIIRELINNEAAVDALKRIATLSDQEKAMHSTLRANEKFDMDELEESPNYTTAENKSIRDYKDTQKALNDARMDFFTEKTKFIAKETLERGGQLYFALDGLVTNSPGFRADTQINMDKLKDVFNPNHEHYDSVTSRELRYLYENYKDNPNLKFTLKDHVIANPLKTLKTSISESDLKSSPRRARQEGPSLLQRVRNLFDKSSSNKRSEKDTAQTSVGYRNTNIDIINDKTKGVNHIVENGVEVALTNTDYKPLKLPNVEAAFKQTKLKAENIDPHIEAVKKLEIIASSANSIPKEHLLKALIEVTEGKTDEDINVYQKLFNTRQNISNEVAPTYSLRNLDGKDGKQILRSVAEIYKNLPLSDTYQAVRNYVNNRLIEKLSSNRLLLEHLANSKISGNEYAIKYIFDTVSRAKQEIFEQELNTELAPVSLDIMRRKAANILSSEHGSYKDGTLSIYDKPVKSAFHSRLKNNGEVLNTIVHELTHHEQDALAKIIDNKGYDAKLFDKNNILYITGGLGYPKQALERDAFLSGDSVSEAFMKKAKEYHERTKQERKDAKKDEARIAKLYKQWEQEEANKKSASLNGSSQSLDSRSEVEFNRVSHKSVR csk_human Tyrosine-protein kinase CSK CSK C-Src kinase Protein-tyrosine kinase CYL protein protein human Homo sapiens Human MSAIQAAWPSGTECIAKYNFHGTAEQDLPFCKGDVLTIVAVTKDPNWYKAKNKVGREGIIPANYVQKREGVKAGTKLSLMPWFHGKITREQAERLLYPPETGLFLVRESTNYPGDYTLCVSCDGKVEHYRIMYHASKLSIDEEVYFENLMQLVEHYTSDADGLCTRLIKPKVMEGTVAAQDEFYRSGWALNMKELKLLQTIGKGEFGDVMLGDYRGNKVAVKCIKNDATAQAFLAEASVMTQLRHSNLVQLLGVIVEEKGGLYIVTEYMAKGSLVDYLRSRGRSVLGGDCLLKFSLDVCEAMEYLEGNNFVHRDLAARNVLVSEDNVAKVSDFGLTKEASSTQDTGKLPVKWTAPEALREKKFSTKSDVWSFGILLWEIYSFGRVPYPRIPLKDVVPRVEKGYKMDAPDGCPPAVYEVMKNCWHLDAAMRPSFLQLREQLEHIKTHELHL nck2_human Cytoplasmic protein NCK2 NCK adaptor protein 2 SH2/SH3 adaptor protein NCK-beta NCK2 GRB4 Growth factor receptor-bound protein 4 protein protein human Homo sapiens Human MTEEVIVIAKWDYTAQQDQELDIKKNERLWLLDDSKTWWRVRNAANRTGYVPSNYVERKNSLKKGSLVKNLKDTLGLGKTRRKTSARDASPTPSTDAEYPANGSGADRIYDLNIPAFVKFAYVAEREDELSLVKGSRVTVMEKCSDGWWRGSYNGQIGWFPSNYVLEEVDEAAAESPSFLSLRKGASLSNGQGSRVLHVVQTLYPFSSVTEEELNFEKGETMEVIEKPENDPEWWKCKNARGQVGLVPKNYVVVLSDGPALHPAHAPQISYTGPSSSGRFAGREWYYGNVTRHQAECALNERGVEGDFLIRDSESSPSDFSVSLKASGKNKHFKVQLVDNVYCIGQRRFHTMDELVEHYKKAPIFTSEHGEKLYLVRALQ grb2_human Growth factor receptor-bound protein 2 GRB2 ASH Adapter protein GRB2 SH2/SH3 adapter GRB2 Protein Ash protein protein human Homo sapiens Human MEAIAKYDFKATADDELSFKRGDILKVLNEECDQNWYKAELNGKDGFIPKNYIEMKPHPWFFGKIPRAKAEEMLSKQRHDGAFLIRESESAPGDFSLSVKFGNDVQHFKVLRDGAGKYFLWVVKFNSLNELVDYHRSTSVSRNQQIFLRDIEQVPQQPTYVQALFDFDPQEDGELGFRRGDFIHVMDNSDPNWWKGACHGQTGMFPRNYVTPVNRNV cdk1_human Cyclin-dependent kinase 1 p34 protein kinase Cell division protein kinase 1 CDK1 CDC2 Cell division control protein 2 homolog CDC28A CDKN1 P34CDC2 protein protein human Homo sapiens Human MEDYTKIEKIGEGTYGVVYKGRHKTTGQVVAMKKIRLESEEEGVPSTAIREISLLKELRHPNIVSLQDVLMQDSRLYLIFEFLSMDLKKYLDSIPPGQYMDSSLVKSYLYQILQGIVFCHSRRVLHRDLKPQNLLIDDKGTIKLADFGLARAFGIPIRVYTHEVVTLWYRSPEVLLGSARYSTPVDIWSIGTIFAELATKKPLFHGDSEIDQLFRIFRALGTPNNEVWPEVESLQDYKNTFPKWKPGSLASHVKNLDENGLDLLSKMLIYDPAKRISGKMALNHPYFNDLDNQIKKM capr1_human Caprin-1 CAPRIN1 GPIAP1 GPIP137 M11S1 Cytoplasmic activation- and proliferation-associated protein 1 GPI-anchored membrane protein 1 GPI-anchored protein p137 Cell cycle-associated protein 1 Membrane component chromosome 11 surface marker 1 RNG105 RNA granule protein 105 protein protein human Homo sapiens Human MPSATSHSGSGSKSSGPPPPSGSSGSEAAAGAGAAAPASQHPATGTGAVQTEAMKQILGVIDKKLRNLEKKKGKLDDYQERMNKGERLNQDQLDAVSKYQEVTNNLEFAKELQRSFMALSQDIQKTIKKTARREQLMREEAEQKRLKTVLELQYVLDKLGDDEVRTDLKQGLNGVPILSEEELSLLDEFYKLVDPERDMSLRLNEQYEHASIHLWDLLEGKEKPVCGTTYKVLKEIVERVFQSNYFDSTHNHQNGLCEEEEAASAPAVEDQVPEAEPEPAEEYTEQSEVESTEYVNRQFMAETQFTSGEKEQVDEWTVETVEVVNSLQQQPQAASPSVPEPHSLTPVAQADPLVRRQRVQDLMAQMQGPYNFIQDSMLDFENQTLDPAIVSAQPMNPTQNMDMPQLVCPPVHSESRLAQPNQVPVQPEATQVPLVSSTSEGYTASQPLYQPSHATEQRPQKEPIDQIQATISLNTDQTTASSSLPAASQPQVFQAGTSKPLHSSGINVNAAPFQSMQTVFNMNAPVPPVNEPETLKQQNQYQASYNQSFSSQPHQVEQTELQQEQLQTVVGTYHGSPDQSHQVTGNHQQPPQQNTGFPRSNQPYYNSRGVSRGGSRGARGLMNGYRGPANGFRGGYDGYRPSFSNTPNSGYTQSQFSAPRDYSGYQRDGYQQNFKRGSGQSGPRGAPRGRGGPPRPNRGMPQMNTQQVN dhx15_human Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15 DHX15 DBP1 DDX15 DEAH box protein 15 ATP-dependent RNA helicase #46 protein protein human Homo sapiens Human MSKRHRLDLGEDYPSGKKRAGTDGKDRDRDRDREDRSKDRDRERDRGDREREREKEKEKELRASTNAMLISAGLPPLKASHSAHSTHSAHSTHSTHSAHSTHAGHAGHTSLPQCINPFTNLPHTPRYYDILKKRLQLPVWEYKDRFTDILVRHQSFVLVGETGSGKTTQIPQWCVEYMRSLPGPKRGVACTQPRRVAAMSVAQRVADEMDVMLGQEVGYSIRFEDCSSAKTILKYMTDGMLLREAMNDPLLERYGVIILDEAHERTLATDILMGVLKEVVRQRSDLKVIVMSATLDAGKFQIYFDNCPLLTIPGRTHPVEIFYTPEPERDYLEAAIRTVIQIHMCEEEEGDLLLFLTGQEEIDEACKRIKREVDDLGPEVGDIKIIPLYSTLPPQQQQRIFEPPPPKKQNGAIGRKVVVSTNIAETSLTIDGVVFVIDPGFAKQKVYNPRIRVESLLVTAISKASAQQRAGRAGRTRPGKCFRLYTEKAYKTEMQDNTYPEILRSNLGSVVLQLKKLGIDDLVHFDFMDPPAPETLMRALELLNYLAALNDDGDLTELGSMMAEFPLDPQLAKMVIASCDYNCSNEVLSITAMLSVPQCFVRPTEAKKAADEAKMRFAHIDGDHLTLLNVYHAFKQNHESVQWCYDNFINYRSLMSADNVRQQLSRIMDRFNLPRRSTDFTSRDYYINIRKALVTGYFMQVAHLERTGHYLTVKDNQVVQLHPSTVLDHKPEWVLYNEFVLTTKNYIRTCTDIKPEWLVKIAPQYYDMSNFPQCEAKRQLDRIIAKLQSKEYSQY nono_human Non-POU domain-containing octamer-binding protein NONO NRB54 54 kDa nuclear RNA- and DNA-binding protein p54(nrb) 55 kDa nuclear protein NMT55 DNA-binding p52/p100 complex, 52 kDa subunit protein protein human Homo sapiens Human MQSNKTFNLEKQNHTPRKHHQHHHQQQHHQQQQQQPPPPPIPANGQQASSQNEGLTIDLKNFRKPGEKTFTQRSRLFVGNLPPDITEEEMRKLFEKYGKAGEVFIHKDKGFGFIRLETRTLAEIAKVELDNMPLRGKQLRVRFACHSASLTVRNLPQYVSNELLEEAFSVFGQVERAVVIVDDRGRPSGKGIVEFSGKPAARKALDRCSEGSFLLTTFPRPVTVEPMDQLDDEEGLPEKLVIKNQQFHKEREQPPRFAQPGSFEYEYAMRWKALIEMEKQQQDQVDRNIKEAREKLEMEMEAARHEHQVMLMRQDLMRRQEELRRMEELHNQEVQKRKQLELRQEEERRRREEEMRRQQEEMMRRQQEGFKGTFPDAREQEIRMGQMAMGGAMGINNRGAMPPAPVPAGTPAPPGPATMMPDGTLGLTPPTTERFGQAATMEGIGAIGGTPPAFNRAAPGAEFAPNKRRRY sfpq_human Splicing factor, proline- and glutamine-rich SFPQ PSF Polypyrimidine tract-binding protein-associated-splicing factor DNA-binding p52/p100 complex, 100 kDa subunit 100 kDa DNA-pairing protein protein protein human Homo sapiens Human MSRDRFRSRGGGGGGFHRRGGGGGRGGLHDFRSPPPGMGLNQNRGPMGPGPGQSGPKPPIPPPPPHQQQQQPPPQQPPPQQPPPHQPPPHPQPHQQQQPPPPPQDSSKPVVAQGPGPAPGVGSAPPASSSAPPATPPTSGAPPGSGPGPTPTPPPAVTSAPPGAPPPTPPSSGVPTTPPQAGGPPPPPAAVPGPGPGPKQGPGPGGPKGGKMPGGPKPGGGPGLSTPGGHPKPPHRGGGEPRGGRQHHPPYHQQHHQGPPPGGPGGRSEEKISDSEGFKANLSLLRRPGEKTYTQRCRLFVGNLPADITEDEFKRLFAKYGEPGEVFINKGKGFGFIKLESRALAEIAKAELDDTPMRGRQLRVRFATHAAALSVRNLSPYVSNELLEEAFSQFGPIERAVVIVDDRGRSTGKGIVEFASKPAARKAFERCSEGVFLLTTTPRPVIVEPLEQLDDEDGLPEKLAQKNPMYQKERETPPRFAQHGTFEYEYSQRWKSLDEMEKQQREQVEKNMKDAKDKLESEMEDAYHEHQANLLRQDLMRRQEELRRMEELHNQEMQKRKEMQLRQEEERRRREEEMMIRQREMEEQMRRQREESYSRMGYMDPRERDMRMGGGGAMNMGDPYGSGGQKFPPLGGGGGIGYEANPGVPPATMSGSMMGSDMRTERFGQGGAGPVGGQGPRGMGPGTPAGYGRGREEYEGPNKKPRF rs23_human 40S ribosomal protein S23 RPS23 Small ribosomal subunit protein uS12 protein protein human Homo sapiens Human MGKCRGLRTARKLRSHRRDQKWHDKQYKKAHLGTALKANPFGGASHAKGIVLEKVGVEAKQPNSAIRKCVRVQLIKNGKKITAFVPNDGCLNFIEENDEVLVAGFGRKGHAVGDIPGVRFKVVKVANVSLLALYKGKKERPRS dhx9_human ATP-dependent RNA helicase A DHX9 DDX9 NDH2 DExH-box helicase 9 RNA helicase A LKP Nuclear DNA helicase II DEAH box protein 9 Leukophysin protein protein human Homo sapiens Human MGDVKNFLYAWCGKRKMTPSYEIRAVGNKNRQKFMCEVQVEGYNYTGMGNSTNKKDAQSNAARDFVNYLVRINEIKSEEVPAFGVASPPPLTDTPDTTANAEGDLPTTMGGPLPPHLALKAENNSEVGASGYGVPGPTWDRGANLKDYYSRKEEQEVQATLESEEVDLNAGLHGNWTLENAKARLNQYFQKEKIQGEYKYTQVGPDHNRSFIAEMTIYIKQLGRRIFAREHGSNKKLAAQSCALSLVRQLYHLGVVEAYSGLTKKKEGETVEPYKVNLSQDLEHQLQNIIQELNLEILPPPEDPSVPVALNIGKLAQFEPSQRQNQVGVVPWSPPQSNWNPWTSSNIDEGPLAFATPEQISMDLKNELMYQLEQDHDLQAILQERELLPVKKFESEILEAISQNSVVIIRGATGCGKTTQVPQFILDDFIQNDRAAECNIVVTQPRRISAVSVAERVAFERGEEPGKSCGYSVRFESILPRPHASIMFCTVGVLLRKLEAGIRGISHVIVDEIHERDINTDFLLVVLRDVVQAYPEVRIVLMSATIDTSMFCEYFFNCPIIEVYGRTYPVQEYFLEDCIQMTHFVPPPKDKKKKDKDDDGGEDDDANCNLICGDEYGPETRLSMSQLNEKETPFELIEALLKYIETLNVPGAVLVFLPGWNLIYTMQKHLEMNPHFGSHRYQILPLHSQIPREEQRKVFDPVPVGVTKVILSTNIAETSITINDVVYVIDSCKQKVKLFTAHNNMTNYATVWASKTNLEQRKGRAGRVRPGFCFHLCSRARFERLETHMTPEMFRTPLHEIALSIKLLRLGGIGQFLAKAIEPPPLDAVIEAEHTLRELDALDANDELTPLGRILAKLPIEPRFGKMMIMGCIFYVGDAICTIAAATCFPEPFINEGKRLGYIHRNFAGNRFSDHVALLSVFQAWDDARMGGEEAEIRFCEHKRLNMATLRMTWEAKVQLKEILINSGFPEDCLLTQVFTNTGPDNNLDVVISLLAFGVYPNVCYHKEKRKILTTEGRNALIHKSSVNCPFSSQDMKYPSPFFVFGEKIRTRAISAKGMTLVTPLQLLLFASKKVQSDGQIVLVDDWIKLQISHEAAACITGLRAAMEALVVEVTKQPAIISQLDPVNERMLNMIRQISRPSAAGINLMIGSTRYGDGPRPPKMARYDNGSGYRRGGSSYSGGGYGGGYSSGGYGSGGYGGSANSFRAGYGAGVGGGYRGVSRGGFRGNSGGDYRGPSGGYRGSGGFQRGGGRGAYGTGYFGQGRGGGGY ptn11_human Tyrosine-protein phosphatase non-receptor type 11 PTPN11 PTP2C SHPTP2 Protein-tyrosine phosphatase 2C Protein-tyrosine phosphatase 1D SH-PTP3 SH-PTP2 protein protein human Homo sapiens Human MTSRRWFHPNITGVEAENLLLTRGVDGSFLARPSKSNPGDFTLSVRRNGAVTHIKIQNTGDYYDLYGGEKFATLAELVQYYMEHHGQLKEKNGDVIELKYPLNCADPTSERWFHGHLSGKEAEKLLTEKGKHGSFLVRESQSHPGDFVLSVRTGDDKGESNDGKSKVTHVMIRCQELKYDVGGGERFDSLTDLVEHYKKNPMVETLGTVLQLKQPLNTTRINAAEIESRVRELSKLAETTDKVKQGFWEEFETLQQQECKLLYSRKEGQRQENKNKNRYKNILPFDHTRVVLHDGDPNEPVSDYINANIIMPEFETKCNNSKPKKSYIATQGCLQNTVNDFWRMVFQENSRVIVMTTKEVERGKSKCVKYWPDEYALKEYGVMRVRNVKESAAHDYTLRELKLSKVGQGNTERTVWQYHFRTWPDHGVPSDPGGVLDFLEEVHHKQESIMDAGPVVVHCSAGIGRTGTFIVIDILIDIIREKGVDCDIDVPKTIQMVRSQRSGMVQTEAQYRFIYMAVQHYIETLQRRIEEEQKSKRKGHEYTNIKYSLADQTSGDQSPLPPCTPTPPCAEMREDSARVYENVGLMQQQKSFR nck1_human Cytoplasmic protein NCK1 NCK1 NCK NCK adaptor protein 1 SH2/SH3 adaptor protein NCK-alpha protein protein human Homo sapiens Human MAEEVVVVAKFDYVAQQEQELDIKKNERLWLLDDSKSWWRVRNSMNKTGFVPSNYVERKNSARKASIVKNLKDTLGIGKVKRKPSVPDSASPADDSFVDPGERLYDLNMPAYVKFNYMAEREDELSLIKGTKVIVMEKCSDGWWRGSYNGQVGWFPSNYVTEEGDSPLGDHVGSLSEKLAAVVNNLNTGQVLHVVQALYPFSSSNDEELNFEKGDVMDVIEKPENDPEWWKCRKINGMVGLVPKNYVTVMQNNPLTSGLEPSPPQCDYIRPSLTGKFAGNPWYYGKVTRHQAEMALNERGHEGDFLIRDSESSPNDFSVSLKAQGKNKHFKVQLKETVYCIGQRKFSTMEELVEHYKKAPIFTSEQGEKLYLVKHLS lspa1-csk-1 3944992 N/A 3944995 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed strep tag strep tag strep tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Q7VLE8:p.[Tyr2469Phe;Tyr2545Phe] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false N/A 3944996 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false binding region binding region binding-associated region binding region range range range range false physical association physical association false false false fig. 3 a-e lspa1-csk-2 3944992 N/A 3944995 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed strep tag strep tag strep tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Q7VLE8:p.[Tyr2469Phe;Tyr2545Phe] mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false N/A 3944997 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false fig. 3b lspa1-nck2-1 3944992 N/A 3944995 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed strep tag strep tag strep tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Q7VLE8:p.[Tyr2469Phe;Tyr2545Phe] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false N/A 3944998 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false fig. 3c lspa1-csk-3 3944992 N/A 3944995 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed strep tag strep tag strep tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Q7VLE8:p.[Tyr2469Phe;Tyr2545Phe] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false N/A 3944996 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false binding region binding region binding-associated region binding region range range range range false physical association physical association false false false fig. 3 e cdk1-csk-1 3944993 N/A 3944995 stimulator stimulator neutral component neutral component binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Q7VLE8:p.[Tyr2469Phe;Tyr2545Phe] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3944999 enzyme target enzyme target substrate substrate neutral component neutral component binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3944996 enzyme enzyme neutral component neutral component spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection phosphorylation phosphorylation reaction phosphorylation false false false fig. 5a To investigate whether YL2 could directly activate Csk, a tyrosine kinase assay that measures phosphorylation of a poly (Glu-Tyr) peptide was performed with recombinant Csk and recombinant YL2, YL2-DM, or L1. Increasing concentrations of YL2 increased Csk activity in a dose-dependent manner (Fig. 5). When 65 nM Csk and 50 nM YL2 were incubated together, therewas a 10-fold increase in Csk activity (Fig. 5A). YL2 itself had no kinase activity, and YL2-DM did not stimulate Csk activity cdk1-csk-2 3944993 N/A 3944995 enzyme target enzyme target substrate substrate neutral component neutral component binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3944996 enzyme enzyme neutral component neutral component spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection phosphorylation phosphorylation reaction phosphorylation false false false fig. 5b To investigate whether YL2 could directly activate Csk, a tyrosine kinase assay that measures phosphorylation of a poly (Glu-Tyr) peptide was performed with recombinant Csk and recombinant YL2, YL2-DM, or L1. Increasing concentrations of YL2 increased Csk activity in a dose-dependent manner (Fig. 5). When 65 nM Csk and 50 nM YL2 were incubated together, therewas a 10-fold increase in Csk activity (Fig. 5A). YL2 itself had no kinase activity, and YL2-DM did not stimulate Csk activity 1-172 3944994 N/A 3945000 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3945001 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3945002 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3945003 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3945004 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3945005 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3944998 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3945006 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3945007 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3944997 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3944996 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3944995 predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false association association false false false table 1