IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ ma-2013-6 human Homo sapiens Human emsa electrophoretic mobility shift assay Gel retardation assay band shift band shift Gel retardation assay emsa weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Ma W., Hu S., Yao G., Xie S., Ni M., Liu Q., Gao X., Zhang J., Huang X., Zhang Y. xingxuhuang@mail.nju.edu.cn The Journal of biological chemistry 2013 Only protein-protein interactions imex curation imex curation ma-2013-3 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. mirna interference luciferase assay miRNA interference luciferase reporter assay miRNA interference luciferase assay tag luciferase tag visualisation by luciferase assay tag luciferase Ma W., Hu S., Yao G., Xie S., Ni M., Liu Q., Gao X., Zhang J., Huang X., Zhang Y. xingxuhuang@mail.nju.edu.cn The Journal of biological chemistry 2013 Only protein-protein interactions imex curation imex curation ma-2013-4 mouse-epididymis mouse epididymis epididymis Epididymis ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Ma W., Hu S., Yao G., Xie S., Ni M., Liu Q., Gao X., Zhang J., Huang X., Zhang Y. xingxuhuang@mail.nju.edu.cn The Journal of biological chemistry 2013 Only protein-protein interactions imex curation imex curation ma-2013-5 mouse-epididymis mouse epididymis epididymis Epididymis ch-ip chromatin immunoprecipitation assay ch-ip q-pcr quantitative pcr q-pcr Ma W., Hu S., Yao G., Xie S., Ni M., Liu Q., Gao X., Zhang J., Huang X., Zhang Y. xingxuhuang@mail.nju.edu.cn The Journal of biological chemistry 2013 Only protein-protein interactions imex curation imex curation andr_human Androgen receptor Dihydrotestosterone receptor Nuclear receptor subfamily 3 group C member 4 AR DHTR NR3C4 protein protein human Homo sapiens Human MEVQLGLGRVYPRPPSKTYRGAFQNLFQSVREVIQNPGPRHPEAASAAPPGASLLLLQQQQQQQQQQQQQQQQQQQQQQQETSPRQQQQQQGEDGSPQAHRRGPTGYLVLDEEQQPSQPQSALECHPERGCVPEPGAAVAASKGLPQQLPAPPDEDDSAAPSTLSLLGPTFPGLSSCSADLKDILSEASTMQLLQQQQQEAVSEGSSSGRAREASGAPTSSKDNYLGGTSTISDNAKELCKAVSVSMGLGVEALEHLSPGEQLRGDCMYAPLLGVPPAVRPTPCAPLAECKGSLLDDSAGKSTEDTAEYSPFKGGYTKGLEGESLGCSGSAAAGSSGTLELPSTLSLYKSGALDEAAAYQSRDYYNFPLALAGPPPPPPPPHPHARIKLENPLDYGSAWAAAAAQCRYGDLASLHGAGAAGPGSGSPSAAASSSWHTLFTAEEGQLYGPCGGGGGGGGGGGGGGGGGGGGGGGEAGAVAPYGYTRPPQGLAGQESDFTAPDVWYPGGMVSRVPYPSPTCVKSEMGPWMDSYSGPYGDMRLETARDHVLPIDYYFPPQKTCLICGDEASGCHYGALTCGSCKVFFKRAAEGKQKYLCASRNDCTIDKFRRKNCPSCRLRKCYEAGMTLGARKLKKLGNLKLQEEGEASSTTSPTEETTQKLTVSHIEGYECQPIFLNVLEAIEPGVVCAGHDNNQPDSFAALLSSLNELGERQLVHVVKWAKALPGFRNLHVDDQMAVIQYSWMGLMVFAMGWRSFTNVNSRMLYFAPDLVFNEYRMHKSRMYSQCVRMRHLSQEFGWLQITPQEFLCMKALLLFSIIPVDGLKNQKFFDELRMNYIKELDRIIACKRKNPTSCSRRFYQLTKLLDSVQPIARELHQFTFDLLIKSHMVSVDFPEMMAEIISVQVPKILSGKVKPIYFHTQ mouse chromosome 6 dna deoxyribonucleic acid DNA deoxyribonucleic acid dna DNA deoxyribonucleic acid mouse Mus musculus Mouse human chromosome 6 human chromosome 6 dna deoxyribonucleic acid DNA deoxyribonucleic acid dna DNA deoxyribonucleic acid synthetic construct synthetic construct hsa-mir-29a-3p mirna micro rna micro RNA micro-RNA micro-rna mirna miRNA human Homo sapiens Human UAGCACCAUCUGAAAUCGGUUA mrna_igf1 insulin like growth factor 1 mrna messenger rna mRNA mrna human Homo sapiens Human CTTCTGTTTGCTAAATCTCACTGTCACTGCTAAATTCAGAGCAGATAGAGCCTGCGCAATGGAATAAAGTCCTCAAAATTGAAATGTGACATTGCTCTCAACATCTCCCATCTCTCTGGATTTCTTTTTGCTTCATTATTCCTGCTAACCAATTCATTTTCAGACTTTGTACTTCAGAAGCAATGGGAAAAATCAGCAGTCTTCCAACCCAATTATTTAAGTGCTGCTTTTGTGATTTCTTGAAGGTGAAGATGCACACCATGTCCTCCTCGCATCTCTTCTACCTGGCGCTGTGCCTGCTCACCTTCACCAGCTCTGCCACGGCTGGACCGGAGACGCTCTGCGGGGCTGAGCTGGTGGATGCTCTTCAGTTCGTGTGTGGAGACAGGGGCTTTTATTTCAACAAGCCCACAGGGTATGGCTCCAGCAGTCGGAGGGCGCCTCAGACAGGCATCGTGGATGAGTGCTGCTTCCGGAGCTGTGATCTAAGGAGGCTGGAGATGTATTGCGCACCCCTCAAGCCTGCCAAGTCAGCTCGCTCTGTCCGTGCCCAGCGCCACACCGACATGCCCAAGACCCAGAAGGAAGTACATTTGAAGAACGCAAGTAGAGGGAGTGCAGGAAACAAGAACTACAGGATGTAGGAAGACCCTCCTGAGGAGTGAAGAGTGACATGCCACCGCAGGATCCTTTGCTCTGCACGAGTTACCTGTTAAACTTTGGAACACCTACCAAAAAATAAGTTTGATAACATTTAAAAGATGGGCGTTTCCCCCAATGAAATACACAAGTAAACATTCCAACATTGTCTTTAGGAGTGATTTGCACCTTGCAAAAATGGTCCTGGAGTTGGTAGATTGCTGTTGATCTTTTATCAATAATGTTCTATAGAAAAGAAAAAAAAAATATATATATATATATATCTTAGTCCCTGCCTCTCAAGAGCCACAAATGCATGGGTGTTGTATAGATCCAGTTGCACTAAATTCCTCTCTGAATCTTGGCTGCTGGAGCCATTCATTCAGCAACCTTGTCTAAGTGGTTTATGAATTGTTTCCTTATTTGCACTTCTTTCTACACAACTCGGGCTGTTTGTTTTACAGTGTCTGATAATCTTGTTAGTCTATACCCACCACCTCCCTTCATAACCTTTATATTTGCCGAATTTGGCCTCCTCAAAAGCAGCAGCAAGTCGTCAAGAAGCACACCAATTCTAACCCACAAGATTCCATCTGTGGCATTTGTACCAAATATAAGTTGGATGCATTTTATTTTAGACACAAAGCTTTATTTTTCCACATCATGCTTACAAAAAAGAATAATGCAAATAGTTGCAACTTTGAGGCCAATCATTTTTAGGCATATGTTTTAAACATAGAAAGTTTCTTCAACTCAAAAGAGTTCCTTCAAATGATGAGTTAATGTGCAACCTAATTAGTAACTTTCCTCTTTTTATTTTTTCCATATAGAGCACTATGTAAATTTAGCATATCAATTATACAGGATATATCAAACAGTATGTAAAACTCTGTTTTTTAGTATAATGGTGCTATTTTGTAGTTTGTTATATGAAAGAGTCTGGCCAAAACGGTAATACGTGAAAGCAAAACAATAGGGGAAGCCTGGAGCCAAAGATGACACAAGGGGAAGGGTACTGAAAACACCATCCATTTGGGAAAGAAGGCAAAGTCCCCCCAGTTATGCCTTCCAAGAGGAACTTCAGACACAAAAGTCCACTGATGCAAATTGGACTGGCGAGTCCAGAGAGGAAACTGTGGAATGGAAAAAGCAGAAGGCTAGGAATTTTAGCAGTCCTGGTTTCTTTTTCTCATGGAAGAAATGAACATCTGCCAGCTGTGTCATGGACTCACCACTGTGTGACCTTGGGCAAGTCACTTCACCTCTCTGTGCCTCAGTTTCCTCATCTGCAAAATGGGGGCAATATGTCATCTACCTACCTCAAAGGGGTGGTATAAGGTTTAAAAAGATAAAGATTCAGATTTTTTTTACCCTGGGTTGCTGTAAGGGTGCAACATCAGGGCGCTTGAGTTGCTGAGATGCAAGGAATTCTATAAATAACCCATTCATAGCATAGCTAGAGATTGGTGAATTGAATGCTCCTGACATCTCAGTTCTTGTCAGTGAAGCTATCCAAATAACTGGCCAACTAGTTGTTAAAAGCTAACAGCTCAATCTCTTAAAACACTTTTCAAAATATGTGGGAAGCATTTGATTTTCAATTTGATTTTGAATTCTGCATTTGGTTTTATGAATACAAAGATAAGTGAAAAGAGAGAAAGGAAAAGAAAAAGGAGAAAAACAAAGAGATTTCTACCAGTGAAAGGGGAATTAATTACTCTTTGTTAGCACTCACTGACTCTTCTATGCAGTTACTACATATCTAGTAAAACCTCGTTTAATACTATAAATAATATTCTATTCATTTTGAAAAACACAATGATTCCTTCTTTTCTAGGCAATATAAGGAAAGTGATCCAAAATTTGAAATATTAAAATAATATCTAATAAAAAGTCACAAAGTTATCTTCTTTAACAAACTTTACTCTTATTCTTAGCTGTATATACATTTTTTTAAAAGTTTGTTAAAATATGCTTGACTAGAGTTTCCAGTTGAAAGGCAAAAACTTCCATCACAACAAGAAATTTCCCATGCCTGCTCAGAAGGGTAGCCCCTAGCTCTCTGTGAATGTGTTTTATCCATTCAACTGAAAATTGGTATCAAGAAAGTCCACTGGTTAGTGTACTAGTCCATCATAGCCTAGAAAATGATCCCTATCTGCAGATCAAGATTTTCTCATTAGAACAATGAATTATCCAGCATTCAGATCTTTCTAGTCACCTTAGAACTTTTTGGTTAAAAGTACCCAGGCTTGATTATTTCATGCAAATTCTATATTTTACATTCTTGGAAAGTCTATATGAAAAACAAAAATAACATCTTCAGTTTTTCTCCCACTGGGTCACCTCAAGGATCAGAGGCCAGGAAAAAAAAAAAAAAGACTCCCTGGATCTCTGAATATATGCAAAAAGAAGGCCCCATTTAGTGGAGCCAGCAATCCTGTTCAGTCAACAAGTATTTTAACTCTCAGTCCAACATTATTTGAATTGAGCACCTCAAGCATGCTTAGCAATGTTCTAATCACTATGGACAGATGTAAAAGAAACTATACATCATTTTTGCCCTCTGCCTGTTTTCCAGACATACAGGTTCTGTGGAATAAGATACTGGACTCCTCTTCCCAAGATGGCACTTCTTTTTATTTCTTGTCCCCAGTGTGTACCTTTTAAAATTATTCCCTCTCAACAAAACTTTATAGGCAGTCTTCTGCAGACTTAACGTGTTTTCTGTCATAGTTAGATGTGATAATTCTAAGAGTGTCTATGACTTATTTCCTTCACTTAATTCTATCCACAGTCAAAAATCCCCCAAGGAGGAAAGCTGAAAGATGCACTGCCATATTATCTTTCTTAACTTTTTCCAACACATAATCCTCTCCAACTGGATTATAAATAAATTGAAAATAACTCATTATACCAATTCACTATTTTATTTTTTAATGAATTAAAACTAGAAAACAAATTGATGCAAACCCTGGAAGTCAGTTGATTACTATATACTACAGCAGAATGACTCAGATTTCATAGAAAGGAGCAACCAAAATGTCACAACCCAAAACTTTACAAGCTTTGCTTCAGAATTAGATTGCTTTATAATTCTTGAATGAGGCAATTTCAAGATATTTGTAAAAGAACAGTAAACATTGGTAAGAATGAGCTTTCAACTCATAGGCTTATTTCCAATTTAATTGACCATACTGGATACTTAGGTCAAATTTCTGTTCTCTCTTCCCCAAATAATATTAAAGTATTATTTGAACTTTTTAAGATGAGGCAGTTCCCCTGAAAAAGTTAATGCAGCTCTCCATCAGAATCCACTCTTCTAGGGATATGAAAATCTCTTAACACCCACCCTACATACACAGACACACACACACACACACACACACACACACACACACACATTCACCCTAAGGATCCAATGGAATACTGAAAAGAAATCACTTCCTTGAAAATTTTATTAAAAAACAAACAAACAAACAAAAAGCCTGTCCACCCTTGAGAATCCTTCCTCTCCTTGGAACGTCAATGTTTGTGTAGATGAAACCATCTCATGCTCTGTGGCTCCAGGGTTTCTGTTACTATTTTATGCACTTGGGAGAAGGCTTAGAATAAAAGATGTAGCACATTTTGCTTTCCCATTTATTGTTTGGCCAGCTATGCCAATGTGGTGCTATTGTTTCTTTAAGAAAGTACTTGACTAAAAAAAAAAGAAAAAAAGAAAAAAAAGAAAGCATAGACATATTTTTTTAAAGTATAAAAACAACAATTCTATAGATAGATGGCTTAATAAAATAGCATTAGGTCTATCTAGCCACCACCACCTTTCAACTTTTTATCACTCACAAGTAGTGTACTGTTCACCAAATTGTGAATTTGGGGGTGCAGGGGCAGGAGTTGGAAATTTTTTAAAGTTAGAAGGCTCCATTGTTTTGTTGGCTCTCAAACTTAGCAAAATTAGCAATATATTATCCAATCTTCTGAACTTGATCAAGAGCATGGAGAATAAACGCGGGAAAAAAGATCTTATAGGCAAATAGAAGAATTTAAAAGATAAGTAAGTTCCTTATTGATTTTTGTGCACTCTGCTCTAAAACAGATATTCAGCAAGTGGAGAAAATAAGAACAAAGAGAAAAAATACATAGATTTACCTGCAAAAAATAGCTTCTGCCAAATCCCCCTTGGGTATTCTTTGGCATTTACTGGTTTATAGAAGACATTCTCCCTTCACCCAGACATCTCAAAGAGCAGTAGCTCTCATGAAAAGCAATCACTGATCTCATTTGGGAAATGTTGGAAAGTATTTCCTTATGAGATGGGGGTTATCTACTGATAAAGAAAGAATTTATGAGAAATTGTTGAAAGAGATGGCTAACAATCTGTGAAGATTTTTTGTTTCTTGTTTTTGTTTTTTTTTTTTTTTTACTTTATACAGTCTTTATGAATTTCTTAATGTTCAAAATGACTTGGTTCTTTTCTTCTTTTTTTATATCAGAATGAGGAATAATAAGTTAAACCCACATAGACTCTTTAAAACTATAGGCTAGATAGAAATGTATGTTTGACTTGTTGAAGCTATAATCAGACTATTTAAAATGTTTTGCTATTTTTAATCTTAAAAGATTGTGCTAATTTATTAGAGCAGAACCTGTTTGGCTCTCCTCAGAAGAAAGAATCTTTCCATTCAAATCACATGGCTTTCCACCAATATTTTCAAAAGATAAATCTGATTTATGCAATGGCATCATTTATTTTAAAACAGAAGAATTGTGAAAGTTTATGCCCCTCCCTTGCAAAGACCATAAAGTCCAGATCTGGTAGGGGGGCAACAACAAAAGGAAAATGTTGTTGATTCTTGGTTTTGGATTTTGTTTTGTTTTCAATGCTAGTGTTTAATCCTGTAGTACATATTTGCTTATTGCTATTTTAATATTTTATAAGACCTTCCTGTTAGGTATTAGAAAGTGATACATAGATATCTTTTTTGTGTAATTTCTATTTAAAAAAGAGAGAAGACTGTCAGAAGCTTTAAGTGCATATGGTACAGGATAAAGATATCAATTTAAATAACCAATTCCTATCTGGAACAATGCTTTTGTTTTTTAAAGAAACCTCTCACAGATAAGACAGAGGCCCAGGGGATTTTTGAAGCTGTCTTTATTCTGCCCCCATCCCAACCCAGCCCTTATTATTTTAGTATCTGCCTCAGAATTTTATAGAGGGCTGACCAAGCTGAAACTCTAGAATTAAAGGAACCTCACTGAAAACATATATTTCACGTGTTCCCTCTTTTTTTTTTTCCTTTTTGTGAGATGGGGTCTCGCACTGTCCCCCAGGCTGGAGTGCAGTGGCATGATCTCGGCTCACTGCAACCTCCACCTCCTGGGTTTAAGCGATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCCACCACTATGCCCGGCTAATTTTTTGGATTTTTAATAGAGACGGGGTTTTACCATGTTGGCCAGGTTGGTCTCAAACTCCTGACCTTGTGATTTGCCCGCCTCAGCCTCCCAAATTGCTGGGATTACAGGCATGAGCCACCACACCCTGCCCATGTGTTCCCTCTTAATGTATGATTACATGGATCTTAAACATGATCCTTCTCTCCTCATTCTTCAACTATCTTTGATGGGGTCTTTCAAGGGGAAAAAAATCCAAGCTTTTTTAAAGTAAAAAAAAAAAAAGAGAGGACACAAAACCAAATGTTACTGCTCAACTGAAATATGAGTTAAGATGGAGACAGAGTTTCTCCTAATAACCGGAGCTGAATTACCTTTCACTTTCAAAAACATGACCTTCCACAATCCTTAGAATCTGCCTTTTTTTATATTACTGAGGCCTAAAAGTAAACATTACTCATTTTATTTTGCCCAAAATGCACTGATGTAAAGTAGGAAAAATAAAAACAGAGCTCTAAAATCCCTTTCAAGCCACCCATTGACCCCACTCACCAACTCATAGCAAAGTCACTTCTGTTAATCCCTTAATCTGATTTTGTTTGGATATTTATCTTGTACCCGCTGCTAAACACACTGCAGGAGGGACTCTGAAACCTCAAGCTGTCTACTTACATCTTTTATCTGTGTCTGTGTATCATGAAAATGTCTATTCAAAATATCAAAACCTTTCAAATATCACGCAGCTTATATTCAGTTTACATAAAGGCCCCAAATACCATGTCAGATCTTTTTGGTAAAAGAGTTAATGAACTATGAGAATTGGGATTACATCATGTATTTTGCCTCATGTATTTTTATCACACTTATAGGCCAAGTGTGATAAATAAACTTACAGACACTGAATTAATTTCCCCTGCTACTTTGAAACCAGAAAATAATGACTGGCCATTCGTTACATCTGTCTTAGTTGAAAAGCATATTTTTTATTAAATTAATTCTGATTGTATTTGAAATTATTATTCAATTCACTTATGGCAGAGGAATATCAATCCTAATGACTTCTAAAAATGTAACTAATTGAATCATTATCTTACATTTACTGTTTAATAAGCATATTTTGAAAATGTATGGCTAGAGTGTCATAATAAAATGGTATATCTTTCTTTAGTAATTACATTAAAATTAGTCATGTTTGATTAA andr_mouse Androgen receptor Ar Nr3c4 Dihydrotestosterone receptor Nuclear receptor subfamily 3 group C member 4 protein protein mouse Mus musculus Mouse MEVQLGLGRVYPRPPSKTYRGAFQNLFQSVREAIQNPGPRHPEAANIAPPGACLQQRQETSPRRRRRQQHTEDGSPQAHIRGPTGYLALEEEQQPSQQQAASEGHPESSCLPEPGAATAPGKGLPQQPPAPPDQDDSAAPSTLSLLGPTFPGLSSCSADIKDILNEAGTMQLLQQQQQQQQHQQQHQQHQQQQEVISEGSSARAREATGAPSSSKDSYLGGNSTISDSAKELCKAVSVSMGLGVEALEHLSPGEQLRGDCMYASLLGGPPAVRPTPCAPLPECKGLPLDEGPGKSTEETAEYSSFKGGYAKGLEGESLGCSGSSEAGSSGTLEIPSSLSLYKSGALDEAAAYQNRDYYNFPLALSGPPHPPPPTHPHARIKLENPLDYGSAWAAAAAQCRYGDLGSLHGGSVAGPSTGSPPATTSSSWHTLFTAEEGQLYGPGGGGGSSSPSDAGPVAPYGYTRPPQGLTSQESDYSASEVWYPGGVVNRVPYPSPNCVKSEMGPWMENYSGPYGDMRLDSTRDHVLPIDYYFPPQKTCLICGDEASGCHYGALTCGSCKVFFKRAAEGKQKYLCASRNDCTIDKFRRKNCPSCRLRKCYEAGMTLGARKLKKLGNLKLQEEGENSNAGSPTEDPSQKMTVSHIEGYECQPIFLNVLEAIEPGVVCAGHDNNQPDSFAALLSSLNELGERQLVHVVKWAKALPGFRNLHVDDQMAVIQYSWMGLMVFAMGWRSFTNVNSRMLYFAPDLVFNEYRMHKSRMYSQCVRMRHLSQEFGWLQITPQEFLCMKALLLFSIIPVDGLKNQKFFDELRMNYIKELDRIIACKRKNPTSCSRRFYQLTKLLDSVQPIARELHQFTFDLLIKSHMVSVDFPEMMAEIISVQVPKILSGKVKPIYFHTQ mmu-mir-29a gene microRNA 29a gene gene gene mouse Mus musculus Mouse ar-mmu_mir_29a gene-3 3724479 N/A 3724483 unspecified role unspecified role prey prey engineered engineered purified purified tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3724484 unspecified role unspecified role bait bait NC_000072.7:g.31071014_31071032delinsgaattaaagcatgttctaa mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false NC_000072.7:g.31071014_31071032delinsgaattaaagcatattttaa mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false NC_000072.7:g.31071014_31071032delinsgaagtacagcatattttaa mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ARE1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Chr 6: 105680-105805 synthetic construct synthetic construct direct interaction direct interaction false false false Fig 3 D, E, F, G human chromosome 6-ar-1 3724479 N/A 3724483 unspecified role unspecified role prey prey engineered engineered purified purified tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3724485 predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified NC_000007.14:g.130890931_130890949delinscgatgaaagcctattttta mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false NC_000007.14:g.130890931C>A mutation mutation certain certain sequence position certain certain certain sequence position certain false ARE1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false synthetic construct synthetic construct direct interaction direct interaction false false false Fig 3 D, E, F, G human chromosome 6-ar-2 3724479 N/A 3724485 predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false NC_000007.14:g.130890931C>A mutation mutation certain certain sequence position certain certain certain sequence position certain false ARE1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false synthetic construct synthetic construct N/A 3724484 competitor competitor competitor unspecified role unspecified role ARE1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Chr 6: 105680-105805 synthetic construct synthetic construct N/A 3724483 unspecified role unspecified role prey prey engineered engineered purified purified tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig 3 D, E, F mouse chromosome 6-ar-1 3724479 N/A 3724483 unspecified role unspecified role prey prey engineered engineered purified purified tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3724484 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ARE1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Chr 6: 105680-105805 synthetic construct synthetic construct N/A 3724485 competitor competitor competitor unspecified role unspecified role region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false NC_000007.14:g.130890931C>A mutation mutation certain certain sequence position certain certain certain sequence position certain false ARE1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false synthetic construct synthetic construct direct interaction direct interaction false false false Fig 3 D, E,G hsa_mir_29a_3p-mrna_igf1-1 3724480 N/A 3724486 predetermined predetermined participant predetermined regulator regulator modulator regulator neutral component neutral component engineered engineered purified purified N/A 3724487 regulator target regulator target regulator target modulator target neutral component neutral component over-expressed over expressed level over-expressed living cell living cell engineered engineered tag luciferase tag luciferase tag 3' range 3' range 3' range 3' range false r.1548_1551delinsgtag mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig 6 J, K down-regulates quantity by destabilization ar-mmu_mir_29a gene-1 3724481 N/A 3724488 antibody detection identification by antibody antibody detection unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3724489 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level AR binding site sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Chr 6: 31070674-31070842 physical association physical association false false false Fig 3 B ar-mmu_mir_29a gene-2 3724482 N/A 3724488 antibody detection identification by antibody antibody detection unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3724489 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level AR binding site sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Chr 6: 31070674-31070842 physical association physical association false false false Fig 3 C testosterone propionate (CHEBI:9466).