IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ rice-2013-1 human-nci_h1299 Homo sapiens nci_h1299 mirna interference luciferase assay miRNA interference luciferase reporter assay miRNA interference luciferase assay tag luciferase tag visualisation by luciferase assay tag luciferase Rice SJ., Lai SC., Wood LW., Helsley KR., Runkle EA., Winslow MM., Mu D. mud@evms. edu The Journal of biological chemistry 2013 Only protein-protein interactions imex curation imex curation rice-2013-2 human-nci_h441 Homo sapiens nci_h441 Human nci-h441cells ch-ip chromatin immunoprecipitation assay ch-ip q-pcr quantitative pcr q-pcr Rice SJ., Lai SC., Wood LW., Helsley KR., Runkle EA., Winslow MM., Mu D. mud@evms. edu The Journal of biological chemistry 2013 Only protein-protein interactions imex curation imex curation hsa-mir-33a-5p mirna micro rna micro RNA micro-RNA micro-rna mirna miRNA human Homo sapiens Human GUGCAUUGUAGUUGCAUUGCA mrna_hmga2 mrna high mobility group AT-hook 2 mrna messenger rna mRNA mrna human Homo sapiens Human CAGCCCGGGATCCCCGGCCTGTCCCTTTAACCCCGCCGCCGGGCGGGAGCACGTGAGCGGGGCTCCGGGTGGCACCCGGGCGCCGCCGCCGCCGAGGCAGTTGTATTTCGAACGCTGCCTCTGGCTAGCAGCCAGGCGCCTTGGCTCGGCGGTCCGCCTGGCCTCCCTCCTCCTCATACTTTTCTTCCTGCGCAACCCCCTCCCCTTTATCCGCCCACGATTAGAGGTGGGCACTCCCCCCACCACCACCCCCTCCCCAAGCGCAAGCGCGTGCACGCACACACACCACACACACTCACACTCACACACACTCACACACACTCATCCCACTTGAATCTTGGGGCAGGAACTCAGAAAACTTCCAGCCCGGGCAGCGCGCGCTTGGTGCAAGACTCAGGAGCTAGCAGCCCGTCCCCCTCCGACTCTCCGGTGCCGCCGCTGCCTGCTCCCGCCACCCTAGGAGGCGCGGTGCCACCCACTACTCTGTCCTCTGCCTGTGCTCCGTGCCCGACCCTATCCCGGCGGAGTCTCCCCATCCTCCTTTGCTTTCCGACTGCCCAAGGCACTTTCAATCTCAATCTCTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCGCAGGGTGGGGGGAAGAGGAGGAGGAATTCTTTCCCCGCCTAACATTTCAAGGGACACAATTCACTCCAAGTCTCTTCCCTTTCCAAGCCGCTTCCGAAGTGCTCCCGGTGCCCGCAACTCCTGATCCCAACCCGCGAGAGGAGCCTCTGCGACCTCAAAGCCTCTCTTCCTTCTCCCTCGCTTCCCTCCTCCTCTTGCTACCTCCACCTCCACCGCCACCTCCACCTCCGGCACCCACCCACCGCCGCCGCCGCCACCGGCAGCGCCTCCTCCTCTCCTCCTCCTCCTCCCCTCTTCTCTTTTTGGCAGCCGCTGGACGTCCGGTGTTGATGGTGGCAGCGGCGGCAGCCTAAGCAACAGCAGCCCTCGCAGCCCGCCAGCTCGCGCTCGCCCCGCCGGCGTCCCCAGCCCTATCACCTCATCTCCCGAAAGGTGCTGGGCAGCTCCGGGGCGGTCGAGGCGAAGCGGCTGCAGCGGCGGTAGCGGCGGCGGGAGGCAGGATGAGCGCACGCGGTGAGGGCGCGGGGCAGCCGTCCACTTCAGCCCAGGGACAACCTGCCGCCCCAGCGCCTCAGAAGAGAGGACGCGGCCGCCCCAGGAAGCAGCAGCAAGAACCAACCGGTGAGCCCTCTCCTAAGAGACCCAGGGGAAGACCCAAAGGCAGCAAAAACAAGAGTCCCTCTAAAGCAGCTCAAAAGAAAGCAGAAGCCACTGGAGAAAAACGGCCAAGAGGCAGACCTAGGAAATGGCCACAACAAGTTGTTCAGAAGAAGCCTGCTCAGGAGGAAACTGAAGAGACATCCTCACAAGAGTCTGCCGAAGAGGACTAGGGGGCGCCAACGTTCGATTTCTACCTCAGCAGCAGTTGGATCTTTTGAAGGGAGAAGACACTGCAGTGACCACTTATTCTGTATTGCCATGGTCTTTCCACTTTCATCTGGGGTGGGGTGGGGTGGGGTGGGGGAGGGGGGGGTGGGGTGGGGAGAAATCACATAACCTTAAAAAGGACTATATTAATCACCTTCTTTGTAATCCCTTCACAGTCCCAGGTTTAGTGAAAAACTGCTGTAAACACAGGGGACACAGCTTAACAATGCAACTTTTAATTACTGTTTTCTTTTTTCTTAACCTACTAATAGTTTGTTGATCTGATAAGCAAGAGTGGGCGGGTGAGAAAAACCGAATTGGGTTTAGTCAATCACTGCACTGCATGCAAACAAGAAACGTGTCACACTTGTGACGTCGGGCATTCATATAGGAAGAACGCGGTGTGTAACACTGTGTACACCTCAAATACCACCCCAACCCACTCCCTGTAGTGAATCCTCTGTTTAGAACACCAAAGATAAGGACTAGATACTACTTTCTCTTTTTCGTATAATCTTGTAGACACTTACTTGATGATTTTTAACTTTTTATTTCTAAATGAGACGAAATGCTGATGTATCCTTTCATTCAGCTAACAAACTAGAAAAGGTTATGTTCATTTTTCAAAAAGGGAAGTAAGCAAACAAATATTGCCAACTCTTCTATTTATGGATATCACACATATCAGCAGGAGTAATAAATTTACTCACAGCACTTGTTTTCAGGACAACACTTCATTTTCAGGAAATCTACTTCCTACAGAGCCAAAATGCCATTTAGCAATAAATAACACTTGTCAGCCTCAGAGCATTTAAGGAAACTAGACAAGTAAAATTATCCTCTTTGTAATTTAATGAAAAGGTACAACAGAATAATGCATGATGAACTCACCTAATTATGAGGTGGGAGGAGCGAAATCTAAATTTCTTTTGCTATAGTTATACATCAATTTAAAAAGCAAAAAAAAAAAAGGGGGGGGCAATCTCTCTCTGTGTCTTTCTCTCTCTCTCTTCCTCTCCCTCTCTCTTTTCATTGTGTATCAGTTTCCATGAAAGACCTGAATACCACTTACCTCAAATTAAGCATATGTGTTACTTCAAGTAATACGTTTTGACATAAGATGGTTGACCAAGGTGCTTTTCTTCGGCTTGAGTTCACCATCTCTTCATTCAAACTGCACTTTTAGCCAGAGATGCAATATATCCCCACTACTCAATACTACCTCTGAATGTTACAACGAATTTACAGTCTAGTACTTATTACATGCTGCTATACACAAGCAATGCAAGAAAAAAACTTACTGGGTAGGTGATTCTAATCATCTGCAGTTCTTTTTGTACACTTAATTACAGTTAAAGAAGCAATCTCCTTACTGTGTTTCAGCATGACTATGTATTTTTCTATGTTTTTTTAATTAAAAATTTTTAAAATACTTGTTTCAGCTTCTCTGCTAGATTTCTACATTAACTTGAAAATTTTTTAACCAAGTCGCTCCTAGGTTCTTAAGGATAATTTTCCTCAATCACACTACACATCACACAAGATTTGACTGTAATATTTAAATATTACCCTCCAAGTCTGTACCTCAAATGAATTCTTTAAGGAGATGGACTAATTGACTTGCAAAGACCTACCTCCAGACTTCAAAAGGAATGAACTTGTTACTTGCAGCATTCATTTGTTTTTTCAATGTTTGAAATAGTTCAAACTGCAGCTAACCCTAGTCAAAACTATTTTTGTAAAAGACATTTGATAGAAAGGAACACGTTTTTACATACTTTTGCAAAATAAGTAAATAATAAATAAAATAAAAGCCAACCTTCAAAGAAACTTGAAGCTTTGTAGGTGAGATGCAACAAGCCCTGCTTTTGCATAATGCAATCAAAAATATGTGTTTTTAAGATTAGTTGAATATAAGAAAATGCTTGACAAATATTTTCATGTATTTTACACAAATGTGATTTTTGTAATATGTCTCAACCAGATTTATTTTAAACGCTTCTTATGTAGAGTTTTTATGCCTTTCTCTCCTAGTGAGTGTGCTGACTTTTTAACATGGTATTATCAACTGGGCCAGGAGGTAGTTTCTCATGACGGCTTTTGTCAGTATGGCTTTTAGTACTGAAGCCAAATGAAACTCAAAACCATCTCTCTTCCAGCTGCTTCAGGGAGGTAGTTTCAAAGGCCACATACCTCTCTGAGACTGGCAGATCGCTCACTGTTGTGAATCACCAAAGGAGCTATGGAGAGAATTAAAACTCAACATTACTGTTAACTGTGCGTTAAATAAGCAAATAAACAGTGGCTCATAAAAATAAAAGTCGCATTCCATATCTTTGGATGGGCCTTTTAGAAACCTCATTGGCCAGCTCATAAAATGGAAGCAATTGCTCATGTTGGCCAAACATGGTGCACCGAGTGATTTCCATCTCTGGTAAAGTTACACTTTTATTTCCTGTATGTTGTACAATCAAAACACACTACTACCTCTTAAGTCCCAGTATACCTCATTTTTCATACTGAAAAAAAAAGCTTGTGGCCAATGGAACAGTAAGAACATCATAAAATTTTTATATATATAGTTTATTTTTGTGGGAGATAAATTTTATAGGACTGTTCTTTGCTGTTGTTGGTCGCAGCTACATAAGACTGGACATTTAACTTTTCTACCATTTCTGCAAGTTAGGTATGTTTGCAGGAGAAAAGTATCAAGACGTTTAACTGCAGTTGACTTTCTCCCTGTTCCTTTGAGTGTCTTCTAACTTTATTCTTTGTTCTTTATGTAGAATTGCTGTCTATGATTGTACTTTGAATCGCTTGCTTGTTGAAAATATTTCTCTAGTGTATTATCACTGTCTGTTCTGCACAATAAACATAACAGCCTCTGTGATCCCCATGTGTTTTGATTCCTGCTCTTTGTTACAGTTCCATTAAATGAGTAATAAAGTTTGGTCAAAACAGATCAA nkx21_human Homeobox protein Nkx-2.1 NKX2-1 TITF1 NKX2A TTF1 Homeobox protein NK-2 homolog A Thyroid transcription factor 1 Thyroid nuclear factor 1 Thyroid-specific enhancer-binding protein protein protein human Homo sapiens Human MSMSPKHTTPFSVSDILSPLEESYKKVGMEGGGLGAPLAAYRQGQAAPPTAAMQQHAVGHHGAVTAAYHMTAAGVPQLSHSAVGGYCNGNLGNMSELPPYQDTMRNSASGPGWYGANPDPRFPAISRFMGPASGMNMSGMGGLGSLGDVSKNMAPLPSAPRRKRRVLFSQAQVYELERRFKQQKYLSAPEREHLASMIHLTPTQVKIWFQNHRYKMKRQAKDKAAQQQLQQDSGGGGGGGGTGCPQQQQAQQQSPRRVAVPVLVKDGKPCQAGAPAPGAASLQGHAQQQAQHQAQAAQAAAAAISVGSGGAGLGAHPGHQPGSAGQSPDLAHHAASPAALQGQVSSLSHLNSSGSDYGTMSCSTLLYGRTW srbp2_human_gene Sterol regulatory element-binding protein 2 SREBF2 BHLHD2 SREBP2 gene gene human Homo sapiens Human tm245_human_gene Transmembrane protein 245 TMEM245 C9orf5 gene gene human Homo sapiens Human hsa_mir_33a_5p-mrna_hmga2-1 3711257 N/A 3711259 predetermined predetermined participant predetermined regulator regulator modulator regulator neutral component neutral component engineered engineered purified purified N/A 3711260 regulator target regulator target regulator target modulator target neutral component neutral component over-expressed over expressed level over-expressed living cell living cell engineered engineered r.[1732_1738delinsggtacc; 2788_2794delinsggtacc] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false r.2788_2794delinsggtacc mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false r.3089_3094delinsggtacc mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false r.[1732_1738delinsggtacc; 3089_3094delinsggtacc] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false tag luciferase tag luciferase tag 3' range 3' range 3' range 3' range false r.[1732_1738delinsggtacc; 2788_2794delinsggtacc;3089_3094delinsggtacc] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false r.1732_1738delinsggtacc mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig 7 A B down-regulates quantity by destabilization nkx2_1-1 3711258 N/A 3711261 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3711262 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Chr 22q12.3-13.1 52388-52452 N/A 3711263 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Chr 9 27488-27589 association association false false false Fig 4 D