UniProt UniProt http://www.uniprot.org castillejo_lpez-2012-1 human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Castillejo-López C., Delgado-Vega AM., Wojcik J., Kozyrev SV., Thavathiru E., Wu YY., Sánchez E., Pöllmann D., López-Egido JR., Fineschi S., Domínguez N., Lu R., James JA., Merrill JT., Kelly JA., Kaufman KM., Moser KL., Gilkeson G., Frostegård J., Pons-Estel BA., D'Alfonso S., Witte T., Callejas JL., Harley JB., Gaffney PM., Martin J., Guthridge JM., Alarcón-Riquelme ME. alarconm@omrf.org Annals of the rheumatic diseases 2012 Only protein-protein interactions imex curation imex curation castillejo_lpez-2012-2 human-daudi Homo sapiens Burkitt's B-cell lymphona cells daudi Homo sapies DAUDI Burkitt's B-cell lymphoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Castillejo-López C., Delgado-Vega AM., Wojcik J., Kozyrev SV., Thavathiru E., Wu YY., Sánchez E., Pöllmann D., López-Egido JR., Fineschi S., Domínguez N., Lu R., James JA., Merrill JT., Kelly JA., Kaufman KM., Moser KL., Gilkeson G., Frostegård J., Pons-Estel BA., D'Alfonso S., Witte T., Callejas JL., Harley JB., Gaffney PM., Martin J., Guthridge JM., Alarcón-Riquelme ME. alarconm@omrf.org Annals of the rheumatic diseases 2012 Only protein-protein interactions imex curation imex curation castillejo_lpez-2012-3 human-b-cell Homo sapiens B cell b-cell B-cell [B lymphocyte; B-lymphocyte; B cell; B-cells] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Castillejo-López C., Delgado-Vega AM., Wojcik J., Kozyrev SV., Thavathiru E., Wu YY., Sánchez E., Pöllmann D., López-Egido JR., Fineschi S., Domínguez N., Lu R., James JA., Merrill JT., Kelly JA., Kaufman KM., Moser KL., Gilkeson G., Frostegård J., Pons-Estel BA., D'Alfonso S., Witte T., Callejas JL., Harley JB., Gaffney PM., Martin J., Guthridge JM., Alarcón-Riquelme ME. alarconm@omrf.org Annals of the rheumatic diseases 2012 Only protein-protein interactions imex curation imex curation castillejo_lpez-2012-4 human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. confocal microscopy confocal microscopy anti-tag immunocytochemistry anti-tag immunocytochemistry Castillejo-López C., Delgado-Vega AM., Wojcik J., Kozyrev SV., Thavathiru E., Wu YY., Sánchez E., Pöllmann D., López-Egido JR., Fineschi S., Domínguez N., Lu R., James JA., Merrill JT., Kelly JA., Kaufman KM., Moser KL., Gilkeson G., Frostegård J., Pons-Estel BA., D'Alfonso S., Witte T., Callejas JL., Harley JB., Gaffney PM., Martin J., Guthridge JM., Alarcón-Riquelme ME. alarconm@omrf.org Annals of the rheumatic diseases 2012 Only protein-protein interactions imex curation imex curation castillejo_lpez-2012-5 human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Castillejo-López C., Delgado-Vega AM., Wojcik J., Kozyrev SV., Thavathiru E., Wu YY., Sánchez E., Pöllmann D., López-Egido JR., Fineschi S., Domínguez N., Lu R., James JA., Merrill JT., Kelly JA., Kaufman KM., Moser KL., Gilkeson G., Frostegård J., Pons-Estel BA., D'Alfonso S., Witte T., Callejas JL., Harley JB., Gaffney PM., Martin J., Guthridge JM., Alarcón-Riquelme ME. alarconm@omrf.org Annals of the rheumatic diseases 2012 Only protein-protein interactions imex curation imex curation blk_human Tyrosine-protein kinase Blk BLK B lymphocyte kinase p55-Blk protein protein human Homo sapiens Human MGLVSSKKPDKEKPIKEKDKGQWSPLKVSAQDKDAPPLPPLVVFNHLTPPPPDEHLDEDKHFVVALYDYTAMNDRDLQMLKGEKLQVLKGTGDWWLARSLVTGREGYVPSNFVARVESLEMERWFFRSQGRKEAERQLLAPINKAGSFLIRESETNKGAFSLSVKDVTTQGELIKHYKIRCLDEGGYYISPRITFPSLQALVQHYSKKGDGLCQRLTLPCVRPAPQNPWAQDEWEIPRQSLRLVRKLGSGQFGEVWMGYYKNNMKVAIKTLKEGTMSPEAFLGEANVMKALQHERLVRLYAVVTKEPIYIVTEYMARGCLLDFLKTDEGSRLSLPRLIDMSAQIAEGMAYIERMNSIHRDLRAANILVSEALCCKIADFGLARIIDSEYTAQEGAKFPIKWTAPEAIHFGVFTIKADVWSFGVLLMEVVTYGRVPYPGMSNPEVIRNLERGYRMPRPDTCPPELYRGVIAECWRSRPEERPTFEFLQSVLEDFYTATERQYELQP bank1_human B-cell scaffold protein with ankyrin repeats BANK1 protein protein human Homo sapiens Human MLPAAPGKGLGSPDPAPCGPAPPGNTKDIIMIYEEDAEEWALYLTEVFLHVVKREAILLYRLENFSFRHLELLNLTSYKCKLLILSNSLLRDLTPKKCQFLEKILHSPKSVVTLLCGVKSSDQLYELLNISQSRWEISTEQEPEDYISVIQSIIFKDSEDYFEVNIPTDLRAKHSGEISERKEIEELSEASRNTIPLAVVLPTEIPCENPGEIFIILRDEVIGDTVEVEFTSSNKRIRTRPALWNKKVWCMKALEFPAGSVHVNVYCDGIVKATTKIKYYPTAKAKECLFRMADSGESLCQNSIEELDGVLTSIFKHEIPYYEFQSLQTEICSQNKYTHFKELPTLLHCAAKFGLKNLAIHLLQCSGATWASKMKNMEGSDPAHIAERHGHKELKKIFEDFSIQEIDINNEQENDYEEDIASFSTYIPSTQNPAFHHESRKTYGQSADGAEANEMEGEGKQNGSGMETKHSPLEVGSESSEDQYDDLYVFIPGADPENNSQEPLMSSRPPLPPPRPVANAFQLERPHFTLPGTMVEGQMERSQNWGHPGVRQETGDEPKGEKEKKEEEKEQEEEEDPYTFAEIDDSEYDMILANLSIKKKTGSRSFIINRPPAPTPRPTSIPPKEETTPYIAQVFQQKTARRQSDDDKFCGLPKKQDRARIESPAFSTLRGCLTDGQEELILLQEKVKNGKMSMDEALEKFKHWQMGKSGLEMIQQEKLRQLRDCIIGKRPEEENVYNKLTIVHHPGGKETAHNENKFYNVHFSNKLPARPQVEKEFGFCCKKDH bank1-blk-1 3948409 N/A 3948414 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed c-terminal v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 3948415 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig. 1A blk-bank1-1 3948409 N/A 3948414 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed c-terminal v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 3948415 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig. 1B blk-bank1-2 3948410 N/A 3948414 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3948415 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false Fig 1C bank1-blk-2 3948411 N/A 3948414 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3948415 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false Fig 1D bank1-blk-3 3948412 N/A 3948414 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed c-terminal v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 3948415 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed colocalization colocalization false false false Fig. 2C bank1-blk-4 3948413 N/A 3948414 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed c-terminal gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 3948415 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed c-terminal mcherry mcherry fluorescent protein tag mcherry c-term range C-terminal range c-term range c-term range C-terminal range c-term range false colocalization colocalization false false false Supp Fig. 1C