MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url&gt;http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint park-2011-12 in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel weight by staining molecular weight estimation by staining weight by staining Park H., Lee JH., Gouin E., Cossart P., Izard T. kernick@scripps.edu <kernick@scripps.edu>; The Journal of biological chemistry 2011 Only protein-protein interactions imex curation imex curation park-2011-14 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Park H., Lee JH., Gouin E., Cossart P., Izard T. kernick@scripps.edu <kernick@scripps.edu>; The Journal of biological chemistry 2011 Only protein-protein interactions imex curation imex curation park-2011-13 mouse-3t3 Mus musculus embryo cells 3t3 Mouse embryo fibroblast cells confocal microscopy confocal microscopy immunostaining immunostaining Park H., Lee JH., Gouin E., Cossart P., Izard T. kernick@scripps.edu <kernick@scripps.edu>; The Journal of biological chemistry 2011 Only protein-protein interactions imex curation imex curation park-2011-15 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Park H., Lee JH., Gouin E., Cossart P., Izard T. kernick@scripps.edu <kernick@scripps.edu>; The Journal of biological chemistry 2011 Only protein-protein interactions imex curation imex curation b0bxr4_ricro 120 kDa antigen Antigenic heat-stable 120 kDa protein Protein PS 120 RrIowa_0797 protein protein Rickettsia rickettsii (strain Iowa) Rickettsia rickettsii (strain Iowa) MRGFMSKDGNLDTSEFDPLANKEYTEEQKQTLEQEQKEFLSQTTTPELEADDGFIVTSESSAQSTPSMSALSGNISPDSQTSDPITKAVRETIIQPQKDNLIEQILKDLAALTDRDLAEQKRKEIEEEKEKDKTLSTFFGNPANREFIDKALEKPELKKKLESIEIAGYKNVHNTFSAASGYPGGFKPVQWENHVSASDLRATVVKNDAGDELCTLNETTVKTKPFTLAKQDGTQVQISSYREIDFPIKLDQADGSMHLSMVALKADGTKPSKDKAVYFTAHYEEGPNGKPQLKEISSPKPLKFAGTGDDAIAYIEHGGEIYTLAVTRGKYKEMMKEVELNQGQSVDLSQAEDIIIGQGQSKEQPLITPQQTTSSSVEPPQYKQQVPPITPTNQPLQPETSQMPQSQHVNPNLLNAATALSGSMQYLLNYVNAGLTKEIDSNQQIDLIKEAATVILNNEKSDIAEKQANIIAFAENTVNNKNLKPDAKVAGVNAVLETIKNDQNTPDLEKSKMLEATVAIVLNSENLEPKQKQQVLDKAVDVGLSLKDDASRAAAIDGIKDVVIKSNLSTVMLIAVGDKVNVSELSNAEKQKLLGSVLKKGVEAQVLSPAQQQLMQQHLDKIMAEQTKKDTIKKVNDILFDPLSNTELKTTNIQAIISNVLDGPATAVVKGEIIQEITNTVAGSSLEAQDKATIVKGVGETIATHSDTSLSLPNKALIMASAEKGIAESQTTLPDRELMTKGLVDGIYEGKGGPEITKAVSSGIDNSNINDSEKEALKKAKDAASETALDRDTQNLTEGLKGQNIEEHKPHDDIYNKAREVINAVNPVIEALEKSKEPVVSAEARIVQETSSILNNISKLAVEKVNNFRAMLSPNGNLKTLEEKKEESIKKVDELVKAFGTKSSTAEQQSFIKTNLIDDKTLSKEVRLQTIDKLLQEQKRAEAIENPSVKTEDVRVVSGKSQLKPISKDNPDIEKAKMVVGRDRVNIKGNIKIMGALMNARDIIQSENLNKSTPIKRESSPPQR vinc_human Vinculin Metavinculin VCL protein protein human Homo sapiens Human MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQAKGWLRDPSASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGSSPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEISALTSKLADLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGKLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKPGIPAAEVGIGVVAEADAADAAGFPVPPDMEDDYEPELLLMPSNQPVNQPILAAAQSLHREATKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ vinc_mouse Vinculin Metavinculin Vcl protein protein mouse Mus musculus Mouse MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQAKGWLRDPNASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGASPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEIAALTSKLGDLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKAQMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGRLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVISAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ b0bxr4_ricro-vcl-3 3931851 N/A 3931855 unspecified role unspecified role neutral component neutral component purified purified binding region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false binding region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3931856 unspecified role unspecified role neutral component neutral component purified purified binding region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false fig.1b b0bxr4_ricro-vcl-5 3931851 N/A 3931855 unspecified role unspecified role unspecified role unspecified role purified purified mbp tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false VBS-N binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false VBS-C binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3931856 unspecified role unspecified role unspecified role unspecified role purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false fig. 1c b0bxr4_ricro-vcl-4 3931851 N/A 3931855 competitor competitor competitor neutral component neutral component purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false N/A 3931856 self self neutral component neutral component purified purified Vh1 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 3931856 self self neutral component neutral component purified purified Vt binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false fig. 2a Indeed, native gel analyses showed that the nearly full-length sca4 (residues 21–1008) as well as the domains harboring the VBSs (residues 411–585 and 774–1008) readily displaced the Vt tail domain from pre-existing Vh1Vt complexes by forming a new Vh1sca4-VBS complex (Fig. 2A). b0bxr4_ricro-vcl-2 3931852 N/A 3931856 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3931855 unspecified role unspecified role neutral component neutral component purified purified VBS-N binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false table 1,2 b0bxr4_ricro-vcl-1 3931852 N/A 3931856 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3931855 unspecified role unspecified role neutral component neutral component purified purified VBS-N binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false table 1,2 b0bxr4_ricro-vcl-6 3931853 N/A 3931857 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell N/A 3931855 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false colocalization colocalization false false false fig. 3 Confocal microscopy established that eGFP-sca4, residues 406–585, 776–1008, or 21–1008 were present in fibrous-like structures and at punctate that overlapped with focal adhesions. Notably, both nearly full-length sca4s and the domains harboring the sca4-VBSs co-localized with endogenous vinculin. b0bxr4_ricro-vcl-7 3931854 n/a 3931856 unspecified role unspecified role neutral component neutral component purified purified Vh1 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli Stoichiometry: 1.0 N/A 3931855 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli Stoichiometry: 1.0 direct interaction direct interaction false false false suppl. fig. 1,2