UniProt UniProt http://www.uniprot.org britzen_laurent-2009-1 human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Britzen-Laurent N., Bauer M., Berton V., Fischer N., Syguda A., Reipschläger S., Naschberger E., Herrmann C., Stürzl M. michael.stuerzl@uk-erlangen.de PloS one 2009 imex curation Only protein-protein interactions imex curation britzen_laurent-2009-2 human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 bifc bimolecular fluorescence complementation bifc tag fluorescence tag visualisation by fluorescence tag fluorescence Britzen-Laurent N., Bauer M., Berton V., Fischer N., Syguda A., Reipschläger S., Naschberger E., Herrmann C., Stürzl M. michael.stuerzl@uk-erlangen.de PloS one 2009 imex curation Only protein-protein interactions imex curation britzen_laurent-2009-3 yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Britzen-Laurent N., Bauer M., Berton V., Fischer N., Syguda A., Reipschläger S., Naschberger E., Herrmann C., Stürzl M. michael.stuerzl@uk-erlangen.de PloS one 2009 imex curation Only protein-protein interactions imex curation gbp1_human Guanylate-binding protein 1 GBP1 GTP-binding protein 1 Guanine nucleotide-binding protein 1 Interferon-induced guanylate-binding protein 1 protein protein human Homo sapiens Human MASEIHMTGPMCLIENTNGRLMANPEALKILSAITQPMVVVAIVGLYRTGKSYLMNKLAGKKKGFSLGSTVQSHTKGIWMWCVPHPKKPGHILVLLDTEGLGDVEKGDNQNDSWIFALAVLLSSTFVYNSIGTINQQAMDQLYYVTELTHRIRSKSSPDENENEVEDSADFVSFFPDFVWTLRDFSLDLEADGQPLTPDEYLTYSLKLKKGTSQKDETFNLPRLCIRKFFPKKKCFVFDRPVHRRKLAQLEKLQDEELDPEFVQQVADFCSYIFSNSKTKTLSGGIQVNGPRLESLVLTYVNAISSGDLPCMENAVLALAQIENSAAVQKAIAHYEQQMGQKVQLPTETLQELLDLHRDSEREAIEVFIRSSFKDVDHLFQKELAAQLEKKRDDFCKQNQEASSDRCSALLQVIFSPLEEEVKAGIYSKPGGYRLFVQKLQDLKKKYYEEPRKGIQAEEILQTYLKSKESMTDAILQTDQTLTEKEKEIEVERVKAESAQASAKMLQEMQRKNEQMMEQKERSYQEHLKQLTEKMENDRVQLLKEQERTLALKLQEQEQLLKEGFQKESRIMKNEIQDLQTKMRRRKACTIS gbp2_human Guanylate-binding protein 2 Guanine nucleotide-binding protein 2 GTP-binding protein 2 GBP2 Interferon-induced guanylate-binding protein 2 protein protein human Homo sapiens Human MAPEINLPGPMSLIDNTKGQLVVNPEALKILSAITQPVVVVAIVGLYRTGKSYLMNKLAGKKNGFSLGSTVKSHTKGIWMWCVPHPKKPEHTLVLLDTEGLGDIEKGDNENDSWIFALAILLSSTFVYNSMGTINQQAMDQLHYVTELTDRIKANSSPGNNSVDDSADFVSFFPAFVWTLRDFTLELEVDGEPITADDYLELSLKLRKGTDKKSKSFNDPRLCIRKFFPKRKCFVFDWPAPKKYLAHLEQLKEEELNPDFIEQVAEFCSYILSHSNVKTLSGGIPVNGPRLESLVLTYVNAISSGDLPCMENAVLALAQIENSAAVEKAIAHYEQQMGQKVQLPTETLQELLDLHRDSEREAIEVFMKNSFKDVDQMFQRKLGAQLEARRDDFCKQNSKASSDCCMALLQDIFGPLEEDVKQGTFSKPGGYRLFTQKLQELKNKYYQVPRKGIQAKEVLKKYLESKEDVADALLQTDQSLSEKEKAIEVERIKAESAEAAKKMLEEIQKKNEEMMEQKEKSYQEHVKQLTEKMERDRAQLMAEQEKTLALKLQEQERLLKEGFENESKRLQKDIWDIQMRSKSLEPICNIL gbp5_human Guanylate-binding protein 5 Guanine nucleotide-binding protein 5 GTP-binding protein 5 GBP-TA antigen GBP5 UNQ2427/PRO4987 protein protein human Homo sapiens Human MALEIHMSDPMCLIENFNEQLKVNQEALEILSAITQPVVVVAIVGLYRTGKSYLMNKLAGKNKGFSVASTVQSHTKGIWIWCVPHPNWPNHTLVLLDTEGLGDVEKADNKNDIQIFALALLLSSTFVYNTVNKIDQGAIDLLHNVTELTDLLKARNSPDLDRVEDPADSASFFPDLVWTLRDFCLGLEIDGQLVTPDEYLENSLRPKQGSDQRVQNFNLPRLCIQKFFPKKKCFIFDLPAHQKKLAQLETLPDDELEPEFVQQVTEFCSYIFSHSMTKTLPGGIMVNGSRLKNLVLTYVNAISSGDLPCIENAVLALAQRENSAAVQKAIAHYDQQMGQKVQLPMETLQELLDLHRTSEREAIEVFMKNSFKDVDQSFQKELETLLDAKQNDICKRNLEASSDYCSALLKDIFGPLEEAVKQGIYSKPGGHNLFIQKTEELKAKYYREPRKGIQAEEVLQKYLKSKESVSHAILQTDQALTETEKKKKEAQVKAEAEKAEAQRLAAIQRQNEQMMQERERLHQEQVRQMEIAKQNWLAEQQKMQEQQMQEQAAQLSTTFQAQNRSLLSELQHAQRTVNNDDPCVLL gbp3_human Guanylate-binding protein 3 GTP-binding protein 3 Guanine nucleotide-binding protein 3 GBP3 protein protein human Homo sapiens Human MAPEIHMTGPMCLIENTNGELVANPEALKILSAITQPVVVVAIVGLYRTGKSYLMNKLAGKNKGFSLGSTVKSHTKGIWMWCVPHPKKPEHTLVLLDTEGLGDVKKGDNQNDSWIFTLAVLLSSTLVYNSMGTINQQAMDQLYYVTELTHRIRSKSSPDENENEDSADFVSFFPDFVWTLRDFSLDLEADGQPLTPDEYLEYSLKLTQGTSQKDKNFNLPRLCIRKFFPKKKCFVFDLPIHRRKLAQLEKLQDEELDPEFVQQVADFCSYIFSNSKTKTLSGGIKVNGPRLESLVLTYINAISRGDLPCMENAVLALAQIENSAAVQKAIAHYDQQMGQKVQLPAETLQELLDLHRVSEREATEVYMKNSFKDVDHLFQKKLAAQLDKKRDDFCKQNQEASSDRCSALLQVIFSPLEEEVKAGIYSKPGGYCLFIQKLQDLEKKYYEEPRKGIQAEEILQTYLKSKESVTDAILQTDQILTEKEKEIEVECVKAESAQASAKMVEEMQIKYQQMMEEKEKSYQEHVKQLTEKMERERAQLLEEQEKTLTSKLQEQARVLKERCQGESTQLQNEIQKLQKTLKKKTKRYMSHKLKI gbp4_human Guanylate-binding protein 4 GTP-binding protein 4 Guanine nucleotide-binding protein 4 GBP4 protein protein human Homo sapiens Human MGERTLHAAVPTPGYPESESIMMAPICLVENQEEQLTVNSKALEILDKISQPVVVVAIVGLYRTGKSYLMNRLAGKRNGFPLGSTVQSETKGIWMWCVPHLSKPNHTLVLLDTEGLGDVEKSNPKNDSWIFALAVLLSSSFVYNSVSTINHQALEQLHYVTELAELIRAKSCPRPDEAEDSSEFASFFPDFIWTVRDFTLELKLDGNPITEDEYLENALKLIPGKNPKIQNSNMPRECIRHFFRKRKCFVFDRPTNDKQYLNHMDEVPEENLERHFLMQSDNFCSYIFTHAKTKTLREGIIVTGKRLGTLVVTYVDAINSGAVPCLENAVTALAQLENPAAVQRAADHYSQQMAQQLRLPTDTLQELLDVHAACEREAIAVFMEHSFKDENHEFQKKLVDTIEKKKGDFVLQNEEASAKYCQAELKRLSEHLTESILRGIFSVPGGHNLYLEEKKQVEWDYKLVPRKGVKANEVLQNFLQSQVVVEESILQSDKALTAGEKAIAAERAMKEAAEKEQELLREKQKEQQQMMEAQERSFQEYMAQMEKKLEEERENLLREHERLLKHKLKVQEEMLKEEFQKKSEQLNKEINQLKEKIESTKNEQLRLLKILDMASNIMIVTLPGASKLLGVGTKYLGSRI gbp1-gbp1-3 3956768 N/A 3956771 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3956771 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 4A gbp1-gbp1-4 3956768 N/A 3956771 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3956771 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Supp Fig. 2A gbp2-gbp2-1 3956768 N/A 3956772 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3956772 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 4A gbp2-gbp2-2 3956768 N/A 3956772 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3956772 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Supp Fig. 2A gbp5-gbp5-1 3956768 N/A 3956773 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3956773 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 4A gbp5-gbp2-1 3956768 N/A 3956773 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3956773 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Supp Fig. 2A gbp1-gbp2-2 3956768 N/A 3956772 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3956771 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 5A gbp1-gbp5-1 3956768 N/A 3956773 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3956771 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 5A gbp2-gbp1-1 3956768 N/A 3956771 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3956772 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 5A gbp2-gbp5-1 3956768 N/A 3956773 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3956772 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 5A gbp5-gbp1-1 3956768 N/A 3956771 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3956773 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 5A gbp5-gbp2-2 3956768 N/A 3956772 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3956773 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 5A gbp1-gbp2-3 3956768 N/A 3956771 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3956772 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Supp Fig. 2B gbp1-gbp5-2 3956768 N/A 3956771 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3956773 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Supp Fig. 2B gbp2-gbp1-2 3956768 N/A 3956772 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3956771 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Supp Fig. 2B gbp2-gbp5-2 3956768 N/A 3956772 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3956773 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Supp Fig. 2B gbp5-gbp1-2 3956768 N/A 3956773 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3956771 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Supp Fig. 2B gbp5-gbp2-3 3956768 N/A 3956773 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3956772 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Supp Fig. 2B gbp1-gbp3-1 3956768 N/A 3956774 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3956771 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Supp Fig. 9A gbp1-gbp4-1 3956768 N/A 3956775 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3956771 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Supp Fig. 9A gbp2-gbp4-1 3956768 N/A 3956775 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3956772 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Supp Fig. 9A gbp2-gbp3-1 3956768 N/A 3956774 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3956772 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Supp Fig. 9A gbp3-gbp3-2 3956768 N/A 3956774 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3956774 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Supp Fig. 9A gbp4-gbp4-1 3956768 N/A 3956775 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3956775 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Supp Fig. 9A gbp5-gbp3-1 3956768 N/A 3956774 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3956773 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Supp. figure 9A gbp5-gbp4-1 3956768 N/A 3956775 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3956773 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Supp. figure 9A gbp1-gbp1-5 3956769 N/A 3956771 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region venus-c venus c-terminal fragment undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3956771 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region venus-n venus n-terminal fragment undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 4B gbp2-gbp2-3 3956769 N/A 3956772 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-c venus c-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 3956772 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-n venus n-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig. 4B gbp5-gbp5-2 3956769 N/A 3956773 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-c venus c-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 3956773 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-n venus n-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig. 4B gbp1-gbp1-6 3956769 N/A 3956771 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-c venus c-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false P32455:p.Lys51Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false N/A 3956771 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-n venus n-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Supp. Fig. 6 gbp2-gbp2-4 3956769 N/A 3956772 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-c venus c-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3956772 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-n venus n-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig. 4C, Supp. Fig. 4 A/B When cells were treated with a farnesyl transferase inhibitor or geranylgeranyl transferase type I inhibitor, proteins could could still dimerize. However the fluorescent signals were evenly distributed throughout the cytoplasm indicating that isoprenylation is necessary for membrane affinity of the dimers. gbp5-gbp5-3 3956769 N/A 3956773 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-c venus c-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3956773 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-n venus n-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig. 4C, Supp. Fig. 4B When cells were treated with a farnesyl transferase inhibitor or geranylgeranyl transferase type I inhibitor, proteins could could still dimerize. However the fluorescent signals were evenly distributed throughout the cytoplasm indicating that isoprenylation is necessary for membrane affinity of the dimers. gbp1-gbp2-4 3956769 N/A 3956772 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-c venus c-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3956771 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-n venus n-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig. 5B gbp1-gbp2-5 3956769 N/A 3956771 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-c venus c-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3956772 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-n venus n-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig. 5B, Supp. 7B Inhibition of GBP-1 farnesylation with a farnesyl transferase inhibitor induced localization to the perinuclear membranes. gbp1-gbp5-3 3956769 N/A 3956773 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-c venus c-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3956771 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-n venus n-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range false P32455:p.Lys51Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 5B, Supp. Fig 5 gbp1-gbp5-4 3956769 N/A 3956771 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-c venus c-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3956773 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-n venus n-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig. 5B, Supp. figure 7B Inhibition of GBP-1 farnesylation with a farnesyl transferase inhibitor induced localization to the perinuclear membranes. gbp2-gbp5-3 3956769 N/A 3956772 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-c venus c-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3956773 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-n venus n-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig. 5B gbp1-gbp1-8 3956769 N/A 3956771 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-c venus c-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3956771 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-n venus n-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range false P32455:p.Lys51Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 4C, Supp. Fig. 5 gbp1-gbp2-6 3956769 N/A 3956772 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-c venus c-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3956771 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed P32455:p.Lys51Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false n venus-n venus n-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Supp. Fig 5 gbp1-gbp5-5 3956769 N/A 3956773 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-c venus c-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3956771 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed P32455:p.Lys51Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false n-terminus venus-n venus n-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Supp. Fig. 5 gbp1-gbp2-7 3956769 N/A 3956771 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-c venus c-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false P32455:p.Lys51Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false N/A 3956772 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-n venus n-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig. 5B, Supp. fig 6 gbp1-gbp5-6 3956769 N/A 3956771 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n venus-c venus c-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false P32455:p.Lys51Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false N/A 3956773 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-n venus n-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Supp. Fig. 6 gbp2-gbp5-5 3956769 N/A 3956773 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-c venus c-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3956772 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-n venus n-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig. 5B, Supp. Fig. 7B Treatment with GGTI (geranylgeranyl transferase type I inhibitor) resulted in a diffuse localization pattern for GBP-2/GBP-5 heterodimers . gbp1-gbp3-2 3956769 N/A 3956774 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-c venus c-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 3956771 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-n venus n-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig. 6 gbp1-gbp4-2 3956769 N/A 3956775 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-c venus c-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 3956771 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-n venus n-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig. 6 gbp2-gbp4-2 3956769 N/A 3956775 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-c venus c-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 3956772 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-n venus n-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig. 6 gbp2-gbp3-2 3956769 N/A 3956774 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-c venus c-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 3956772 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-n venus n-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig. 6 gbp3-gbp5-1 3956769 N/A 3956774 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-c venus c-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 3956773 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-n venus n-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig. 6 gbp4-gbp5-1 3956769 N/A 3956775 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-c venus c-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 3956773 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-n venus n-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig. 6 gbp2-gbp5-4 3956769 N/A 3956773 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-c venus c-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 3956772 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus venus-n venus n-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Supp. Fig. 7A Treatment with GGTI (geranylgeranyl transferase type I inhibitor) resulted in a diffuse localization pattern for GBP-2/GBP-5 heterodimers . gbp1-gbp1-7 3956770 N/A 3956771 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 3956771 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association false false false Supp. Fig. 3A gbp2-gbp2-5 3956770 N/A 3956772 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 3956772 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association false false false Supp. Fig. 3A gbp5-gbp5-4 3956770 N/A 3956773 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 3956773 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association false false false Supp. Fig. 3A gbp3-gbp3-1 3956770 N/A 3956774 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 3956774 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association false false false Supp. Fig. 3A gbp4-gbp3-1 3956770 N/A 3956775 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 3956775 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association false false false Supp. Fig. 3A