IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ kobayashi-2007-1 mouse-melan-a Mouse melan-a - immortalised melanocyte cell line melan-a Mouse melan-a - immortalised melanocyte cell line crosslink cross-linking study crosslink western blot western blot Immuno blot Immuno blot Kobayashi T., Hearing VJ. Interaction of Tyr with Tyrp1 was found to be disrupted in Tyrp1b mutant melanocytes (Fig 7) Crosslinker: DSP [dithiobis(succinimidylpropionate)] The interacting pair was immunoprecipitated and the proteins were analysed by SDS-PAGE and western blot hearingv@nih.gov Journal of cell science 2007 Only protein-protein interactions imex curation imex curation Dr. P Sergouniotis PhD University of Manchester, UK kobayashi-2007-2 mouse-melan-a Mouse melan-a - immortalised melanocyte cell line melan-a Mouse melan-a - immortalised melanocyte cell line crosslink cross-linking study crosslink western blot western blot Immuno blot Immuno blot Kobayashi T., Hearing VJ. Crosslinker: DSP [dithiobis(succinimidylpropionate)] The interacting partners were immunoprecipitated and subjected to two dimensional SDS-PAGE (nonreducing conditions and further under reducing conditions), followed by western blotting hearingv@nih.gov Journal of cell science 2007 Only protein-protein interactions imex curation imex curation Dr. P Sergouniotis PhD University of Manchester, UK kobayashi-2007-3 mouse-melan-a Mouse melan-a - immortalised melanocyte cell line melan-a Mouse melan-a - immortalised melanocyte cell line confocal microscopy confocal microscopy immunostaining immunostaining Kobayashi T., Hearing VJ. hearingv@nih.gov Journal of cell science 2007 Only protein-protein interactions imex curation imex curation Dr. P Sergouniotis PhD University of Manchester, UK tyro_mouse Tyrosinase Monophenol monooxygenase Albino locus protein Tyr protein protein mouse Mus musculus Mouse MFLAVLYCLLWSFQISDGHFPRACASSKNLLAKECCPPWMGDGSPCGQLSGRGSCQDILLSSAPSGPQFPFKGVDDRESWPSVFYNRTCQCSGNFMGFNCGNCKFGFGGPNCTEKRVLIRRNIFDLSVSEKNKFFSYLTLAKHTISSVYVIPTGTYGQMNNGSTPMFNDINIYDLFVWMHYYVSRDTLLGGSEIWRDIDFAHEAPGFLPWHRLFLLLWEQEIRELTGDENFTVPYWDWRDAENCDICTDEYLGGRHPENPNLLSPASFFSSWQIICSRSEEYNSHQVLCDGTPEGPLLRNPGNHDKAKTPRLPSSADVEFCLSLTQYESGSMDRTANFSFRNTLEGFASPLTGIADPSQSSMHNALHIFMNGTMSQVQGSANDPIFLLHHAFVDSIFEQWLRRHRPLLEVYPEANAPIGHNRDSYMVPFIPLYRNGDFFITSKDLGYDYSYLQESDPGFYRNYIEPYLEQASRIWPWLLGAALVGAVIAAALSGLSSRLCLQKKKKKKQPQEERQPLLMDKDDYHSLLYQSHL tyrp1_mouse 5,6-dihydroxyindole-2-carboxylic acid oxidase Tyrosinase-related protein 1 Catalase B Brown locus protein Tyrp1 Tyrp-1 protein protein mouse Mus musculus Mouse MKSYNVLPLAYISLFLMLFYQVWAQFPRECANIEALRRGVCCPDLLPSSGPGTDPCGSSSGRGRCVAVIADSRPHSRHYPHDGKDDREAWPLRFFNRTCQCNDNFSGHNCGTCRPGWRGAACNQKILTVRRNLLDLSPEEKSHFVRALDMAKRTTHPQFVIATRRLEDILGPDGNTPQFENISVYNYFVWTHYYSVKKTFLGTGQESFGDVDFSHEGPAFLTWHRYHLLQLERDMQEMLQEPSFSLPYWNFATGKNVCDVCTDDLMGSRSNFDSTLISPNSVFSQWRVVCESLEEYDTLGTLCNSTEGGPIRRNPAGNVGRPAVQRLPEPQDVTQCLEVRVFDTPPFYSNSTDSFRNTVEGYSAPTGKYDPAVRSLHNLAHLFLNGTGGQTHLSPNDPIFVLLHTFTDAVFDEWLRRYNADISTFPLENAPIGHNRQYNMVPFWPPVTNTEMFVTAPDNLGYAYEVQWPGQEFTVSEIITIAVVAALLLVAAIFGVASCLIRSRSTKNEANQPLLTDHYQRYAEDYEELPNPNHSMV tyr-tyrp1-1 3657686 N/A 3657689 unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3657690 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false Fig. 2, 4b, 6 Cells were also treated with cycloheximide (fig. 4b) to study its effect and apparently cycloheximide slightly decreased the level of coprecipitated proteins tyr-tyrp1-2 3657686 N/A 3657689 unspecified role unspecified role bait bait subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3657690 unspecified role unspecified role prey prey subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false Fig. 3 tyr-tyrp1-4 3657686 N/A 3657689 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3657690 unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false Fig. 2 Cells were also treated with cycloheximide (fig. 4b) to study its effect and apparently cycloheximide slightly decreased the level of coprecipitated proteins tyr-tyrp1-5 3657686 N/A 3657689 unspecified role unspecified role prey prey subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3657690 unspecified role unspecified role bait bait subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false Fig. 3 lanes 7-9 and Fig. 6 tyr-tyrp1-3 3657687 N/A 3657689 unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3657690 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false Fig. 5 tyr-tyrp1-6 3657688 N/A 3657689 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell N/A 3657690 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell colocalization colocalization false false false 1b, 4a