IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ cortesio-2006-1 in vitro In vitro protease assay protease assay predetermined predetermined participant predetermined Cortesio CL., Jiang W. Amidolytic activity using a fluorometric assay based on the measurement of AMC (aminomethylcoumarin) released upon cleavage. Activity was measured in units -pmol/min/ug enzyme. Peptide sequences are given in Table 1. weipingj@rndsystems.com Archives of biochemistry and biophysics 2006 Only protein-protein interactions imex curation imex curation cortesio-2006-2 in vitro In vitro protease assay protease assay experimental particp experimental participant identification experimental particp Cortesio CL., Jiang W. N-terminal sequencing has been assumed to have been carried out by Edman degradation as no other method was found when searching for this term and "Molecular Structure Facility, University of California, Davis". IGFBP-5 is of Human origin as deduced by specific residues given- notably sequence TAHPRIISAP. weipingj@rndsystems.com Archives of biochemistry and biophysics 2006 Only protein-protein interactions imex curation imex curation p48740-2 Mannan-binding lectin serine protease 1 Mannose-binding protein-associated serine protease Ra-reactive factor serine protease p100 Serine protease 5 MASP-3 MASP1 CRARF CRARF1 PRSS5 Complement factor MASP-3 Complement-activating component of Ra-reactive factor Mannose-binding lectin-associated serine protease 1 protein protein human Homo sapiens Human MRWLLLYYALCFSLSKASAHTVELNNMFGQIQSPGYPDSYPSDSEVTWNITVPDGFRIKLYFMHFNLESSYLCEYDYVKVETEDQVLATFCGRETTDTEQTPGQEVVLSPGSFMSITFRSDFSNEERFTGFDAHYMAVDVDECKEREDEELSCDHYCHNYIGGYYCSCRFGYILHTDNRTCRVECSDNLFTQRTGVITSPDFPNPYPKSSECLYTIELEEGFMVNLQFEDIFDIEDHPEVPCPYDYIKIKVGPKVLGPFCGEKAPEPISTQSHSVLILFHSDNSGENRGWRLSYRAAGNECPELQPPVHGKIEPSQAKYFFKDQVLVSCDTGYKVLKDNVEMDTFQIECLKDGTWSNKIPTCKIVDCRAPGELEHGLITFSTRNNLTTYKSEIKYSCQEPYYKMLNNNTGIYTCSAQGVWMNKVLGRSLPTCLPECGQPSRSLPSLVKRIIGGRNAEPGLFPWQALIVVEDTSRVPNDKWFGSGALLSASWILTAAHVLRSQRRDTTVIPVSKEHVTVYLGLHDVRDKSGAVNSSAARVVLHPDFNIQNYNHDIALVQLQEPVPLGPHVMPVCLPRLEPEGPAPHMLGLVAGWGISNPNVTVDEIISSGTRTLSDVLQYVKLPVVPHAECKTSYESRSGNYSVTENMFCAGYYEGGKDTCLGDSGGAFVIFDDLSQRWVVQGLVSWGGPEECGSKQVYGVYTKVSNYVDWVWEQMGLPQSVVEPQVER pro-phe-arg peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) PFR val-pro-arg peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) VPR gly-gly-arg peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) GGR phe-arg peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) FR leu-arg peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) LR p23827-pro_0000007423 Ecotin eco eti b2209 JW2197 protein protein ecoli Escherichia coli (strain K12) AESVQPLEKIAPYPQAEKGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFDKVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAVVR 21 162 ipsp_human Plasma serine protease inhibitor Serpin A5 Protein C inhibitor Plasminogen activator inhibitor 3 Acrosomal serine protease inhibitor SERPINA5 PCI PLANH3 PROCI protein protein human Homo sapiens Human MQLFLLLCLVLLSPQGASLHRHHPREMKKRVEDLHVGATVAPSSRRDFTFDLYRALASAAPSQSIFFSPVSISMSLAMLSLGAGSSTKMQILEGLGLNLQKSSEKELHRGFQQLLQELNQPRDGFQLSLGNALFTDLVVDLQDTFVSAMKTLYLADTFPTNFRDSAGAMKQINDYVAKQTKGKIVDLLKNLDSNAVVIMVNYIFFKAKWETSFNHKGTQEQDFYVTSETVVRVPMMSREDQYHYLLDRNLSCRVVGVPYQGNATALFILPSEGKMQQVENGLSEKTLRKWLKMFKKRQLELYLPKFSIEGSYQLEKVLPSLGISNVFTSHADLSGISNHSNIQVSEMVHKAVVEVDESGTRAAAATGTIFTFRSARLNSQRLVFNRPFLMFIVDNNILFLGKVNRP p00974-pro_0000016853 Pancreatic trypsin inhibitor Aprotinin Basic protease inhibitor protein protein bovin Bos taurus (Bovine) Bovine RPDFCLEPPYTGPCKARIIRYFYNAKAGLCQTFVYGGCRAKRNNFKSAEDCMRTCGGA 36 93 p03973-pro_0000041355 Antileukoproteinase SLPI WAP4 WFDC4 BLPI HUSI-1 Mucus proteinase inhibitor Protease inhibitor WAP4 Secretory leukocyte protease inhibitor Seminal proteinase inhibitor WAP four-disulfide core domain protein 4 protein protein human Homo sapiens Human SGKSFKAGVCPPKKSAQCLRYKKPECQSDWQCPGKKRCCPDTCGIKCLDPVDTPNPTRRKPGKCPVTYGQCLMLNPPNFCEMDGQCKRDLKCCMGMCGKSCVSPVKA 26 132 aebsf 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) 3,4-dichloroisocoumarin 3,4-dichloroisocoumarin 3,4-DCI 3,4-dichloro-1H-2-benzopyran-1-one 3,4-dichloro-2-benzopyran-1-one 3,4-dichloroisochromen-1-one 3,4-dichloro-1H-isochromen-1-one small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) e64 E64 (2S,3S)-3-(N-{(S)-1-[N-(4-guanidinobutyl)carbamoyl]3-methylbutyl}carbamoyl)oxirane-2-carboxylic acid (2S,3S)-3-[((1S)-1-{[4-(carbamimidamido)butyl]carbamoyl}-3-methylbutyl)carbamoyl]oxirane-2-carboxylic acid E 64 E-64 small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) ethylenediaminetetraacetic acid ethylenediaminetetraacetic acid (ethylenedinitrilo)tetraacetic acid, ion(4-) 2,2',2'',2'''-(ethane-1,2-diyldinitrilo)tetraacetate acide edetique Acide ethylenediaminetetracetique acido edetico acidum edeticum Edetic acid edetic acid EDTA edta EDTA, ion(4-) ethylenediaminetetraacetate ethylenediaminetetraacetic acid Ethylenediaminetetraacetic acid H4edta N,N'-1,2-Ethane diylbis-(N-(carboxymethyl)glycine) {[-(BIS-CARBOXYMETHYL-AMINO)-ETHYL]-CARBOXYMETHYL-AMINO}-ACETIC ACID (ethane-1,2-diyldinitrilo)tetraacetate (ethane-1,2-diyldinitrilo)tetraacetic acid small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) ibp5_human Insulin-like growth factor-binding protein 5 IGFBP5 IBP5 protein protein human Homo sapiens Human MVLLTAVLLLLAAYAGPAQSLGSFVHCEPCDEKALSMCPPSPLGCELVKEPGCGCCMTCALAEGQSCGVYTERCAQGLRCLPRQDEEKPLHALLHGRGVCLNEKSYREQVKIERDSREHEEPTTSEMAEETYSPKIFRPKHTRISELKAEAVKKDRRKKLTQSKFVGGAENTAHPRIISAPEMRQESEQGPCRRHMEASLQELKASPRMVPRAVYLPNCDRKGFYKRKQCKPSRGRKRGICWCVDKYGMKLPGMEYVDGDFQCHTFDSSNVE masp1-pfr-2 3961214 N/A 3961216 enzyme enzyme neutral component neutral component purified purified catalytic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false mouse-NS0 Mouse Myeloma subclone of NS-1 ns0 NS0 Mouse Myeloma subclone of NS-1 http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=85110503&collection=ecacc_gc from ecacc N/A 3961217 enzyme target enzyme target substrate substrate neutral component neutral component C-terminus coumarin label coumarin label c-term range C-terminal range c-term range c-term range C-terminal range c-term range false chemical synthesis Chemical synthesis (Chemical synthesis) protein cleavage protein cleavage false false false Table 1 Activity=11.0 pmoles/min/ug masp1-vpr-2 3961214 N/A 3961216 enzyme enzyme neutral component neutral component purified purified catalytic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false mouse-NS0 Mouse Myeloma subclone of NS-1 ns0 NS0 Mouse Myeloma subclone of NS-1 http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=85110503&collection=ecacc_gc from ecacc N/A 3961218 enzyme target enzyme target substrate substrate neutral component neutral component C-terminus coumarin label coumarin label c-term range C-terminal range c-term range c-term range C-terminal range c-term range false t-Butyloxycarbonyl modval modified L-valine residue ModVal certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) protein cleavage protein cleavage false false false Table 1 Activity = 9.0 pmoles/min/ug ggr-masp1-2 3961214 N/A 3961216 enzyme enzyme neutral component neutral component purified purified catalytic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false mouse-NS0 Mouse Myeloma subclone of NS-1 ns0 NS0 Mouse Myeloma subclone of NS-1 http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=85110503&collection=ecacc_gc from ecacc N/A 3961219 enzyme target enzyme target substrate substrate neutral component neutral component C-terminus coumarin label coumarin label c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Benzyloxycarbonyl modgly modified glycine residue ModGly certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) protein cleavage protein cleavage false false false Table 1 Activity = 5.6 pmoles/min/ug masp1-phe_arg-1 3961214 N/A 3961216 enzyme enzyme neutral component neutral component purified purified catalytic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false mouse-NS0 Mouse Myeloma subclone of NS-1 ns0 NS0 Mouse Myeloma subclone of NS-1 http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=85110503&collection=ecacc_gc from ecacc N/A 3961220 enzyme target enzyme target substrate substrate neutral component neutral component C-terminus coumarin label coumarin label c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Benzyloxycarbonyl modphe modified L-phenylalanine residue ModPhe certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) protein cleavage protein cleavage false false false Table 1 Activity = 1.5 pmoles/min/ug lr-masp1-1 3961214 N/A 3961216 enzyme enzyme neutral component neutral component purified purified catalytic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false mouse-NS0 Mouse Myeloma subclone of NS-1 ns0 NS0 Mouse Myeloma subclone of NS-1 http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=85110503&collection=ecacc_gc from ecacc N/A 3961221 enzyme target enzyme target substrate substrate neutral component neutral component C-terminus coumarin label coumarin label c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Benzyloxycarbonyl modleu modified L-leucine residue ModLeu certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) protein cleavage protein cleavage false false false Table 1 Activity = 1.2 pmoles/min/ug eco-masp1-5 3961214 N/A 3961222 inhibitor inhibitor unspecified role unspecified role purified purified C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) N/A 3961216 enzyme enzyme neutral component neutral component purified purified catalytic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false mouse-NS0 Mouse Myeloma subclone of NS-1 ns0 NS0 Mouse Myeloma subclone of NS-1 http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=85110503&collection=ecacc_gc from ecacc N/A 3961218 enzyme target enzyme target substrate substrate neutral component neutral component C-terminus coumarin label coumarin label c-term range C-terminal range c-term range c-term range C-terminal range c-term range false t-Butyloxycarbonyl modval modified L-valine residue ModVal certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) protein cleavage protein cleavage false false false Table 2 Ecotin % inhibition = 98. masp1-serpina5-1 3961214 N/A 3961223 inhibitor inhibitor unspecified role unspecified role purified purified C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false mouse-nso NSO-mouse myeloma subclone of NS-1 ns0 NS0 Mouse Myeloma subclone of NS-1 http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=85110503&collection=ecacc_gc from ecacc N/A 3961216 enzyme enzyme neutral component neutral component purified purified catalytic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false mouse-NS0 Mouse Myeloma subclone of NS-1 ns0 NS0 Mouse Myeloma subclone of NS-1 http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=85110503&collection=ecacc_gc from ecacc N/A 3961218 enzyme target enzyme target substrate substrate neutral component neutral component C-terminus coumarin label coumarin label c-term range C-terminal range c-term range c-term range C-terminal range c-term range false t-Butyloxycarbonyl modval modified L-valine residue ModVal certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) protein cleavage protein cleavage false false false Table 2 SerpinA5 % inhibition = 30. masp1-pro_0000016853-1 3961214 N/A 3961224 inhibitor inhibitor unspecified role unspecified role purified purified bovin-lung Bovin lung -Cow lung lung N/A 3961216 enzyme enzyme neutral component neutral component purified purified catalytic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false mouse-NS0 Mouse Myeloma subclone of NS-1 ns0 NS0 Mouse Myeloma subclone of NS-1 http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=85110503&collection=ecacc_gc from ecacc N/A 3961218 enzyme target enzyme target substrate substrate neutral component neutral component C-terminus coumarin label coumarin label c-term range C-terminal range c-term range c-term range C-terminal range c-term range false t-Butyloxycarbonyl modval modified L-valine residue ModVal certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) protein cleavage protein cleavage false false false Table 2 Aprotinin % inhibition = 8. masp1-pro_0000016853-2 3961214 N/A 3961225 inhibitor inhibitor unspecified role unspecified role purified purified ecoli Escherichia coli (strain K12) N/A 3961216 enzyme enzyme neutral component neutral component purified purified catalytic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false mouse-NS0 Mouse Myeloma subclone of NS-1 ns0 NS0 Mouse Myeloma subclone of NS-1 http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=85110503&collection=ecacc_gc from ecacc N/A 3961218 enzyme target enzyme target substrate substrate neutral component neutral component C-terminus coumarin label coumarin label c-term range C-terminal range c-term range c-term range C-terminal range c-term range false t-Butyloxycarbonyl modval modified L-valine residue ModVal certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) protein cleavage protein cleavage false false false Table 2 SLP1 % inhibition = 13. aebsf-masp1-1 3961214 N/A 3961226 inhibitor inhibitor unspecified role unspecified role purified purified chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3961216 enzyme enzyme neutral component neutral component purified purified catalytic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false mouse-NS0 Mouse Myeloma subclone of NS-1 ns0 NS0 Mouse Myeloma subclone of NS-1 http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=85110503&collection=ecacc_gc from ecacc N/A 3961218 enzyme target enzyme target substrate substrate neutral component neutral component C-terminus coumarin label coumarin label c-term range C-terminal range c-term range c-term range C-terminal range c-term range false t-Butyloxycarbonyl modval modified L-valine residue ModVal certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) protein cleavage protein cleavage false false false Table 2 AEBSF % inhibition = 98. 3,4_dichloroisocoumarin-masp1-1 3961214 N/A 3961227 inhibitor inhibitor unspecified role unspecified role purified purified chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3961216 enzyme enzyme neutral component neutral component purified purified catalytic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false mouse-NS0 Mouse Myeloma subclone of NS-1 ns0 NS0 Mouse Myeloma subclone of NS-1 http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=85110503&collection=ecacc_gc from ecacc N/A 3961218 enzyme target enzyme target substrate substrate neutral component neutral component C-terminus coumarin label coumarin label c-term range C-terminal range c-term range c-term range C-terminal range c-term range false t-Butyloxycarbonyl modval modified L-valine residue ModVal certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) protein cleavage protein cleavage false false false Table 2 AEBSF % inhibition = 100. e64-masp1-1 3961214 N/A 3961228 inhibitor inhibitor unspecified role unspecified role purified purified chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3961216 enzyme enzyme neutral component neutral component purified purified catalytic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false mouse-NS0 Mouse Myeloma subclone of NS-1 ns0 NS0 Mouse Myeloma subclone of NS-1 http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=85110503&collection=ecacc_gc from ecacc N/A 3961218 enzyme target enzyme target substrate substrate neutral component neutral component C-terminus coumarin label coumarin label c-term range C-terminal range c-term range c-term range C-terminal range c-term range false t-Butyloxycarbonyl modval modified L-valine residue ModVal certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) protein cleavage protein cleavage false false false Table 2 E-64 % inhibition = 17. ethylenediaminetetraacetic acid-masp1-1 3961214 N/A 3961229 inhibitor inhibitor unspecified role unspecified role purified purified chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3961216 enzyme enzyme neutral component neutral component purified purified catalytic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false mouse-NS0 Mouse Myeloma subclone of NS-1 ns0 NS0 Mouse Myeloma subclone of NS-1 http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=85110503&collection=ecacc_gc from ecacc N/A 3961218 enzyme target enzyme target substrate substrate neutral component neutral component C-terminus coumarin label coumarin label c-term range C-terminal range c-term range c-term range C-terminal range c-term range false t-Butyloxycarbonyl modval modified L-valine residue ModVal certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) protein cleavage protein cleavage false false false Table 2 E-64 % inhibition = 21. igfbp5-masp1-1 3961215 N/A 3961216 enzyme enzyme neutral component neutral component purified purified catalytic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false mouse-NS0 Mouse Myeloma subclone of NS-1 ns0 NS0 Mouse Myeloma subclone of NS-1 http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=85110503&collection=ecacc_gc from ecacc N/A 3961230 weight by staining molecular weight estimation by staining weight by staining edman degradation edman degradation enzyme target enzyme target substrate substrate neutral component neutral component arg176-ile177 biological feature biological feature certain certain sequence position certain certain certain sequence position certain false lys140-his141 biological feature biological feature certain certain sequence position certain certain certain sequence position certain false lys135-ile136 biological feature biological feature certain certain sequence position certain certain certain sequence position certain false protein cleavage protein cleavage false false false Figure 2, 3, 4.