DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ horsefield-2019-2 ecoli Escherichia coli (strain K12) molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column experimental particp experimental participant identification experimental particp Horsefield S., Burdett H., Zhang X., Manik MK., Shi Y., Chen J., Qi T., Gilley J., Lai JS., Rank MX., Casey LW., Gu W., Ericsson DJ., Foley G., Hughes RO., Bosanac T., von Itzstein M., Rathjen JP., Nanson JD., Boden M., Dry IB., Williams SJ., Staskawicz BJ., Coleman MP., Ve T., Dodds PN., Kobe B. b.kobe@uq.edu.au Science (New York, N.Y.) 2019 imex curation Only protein-protein interactions imex curation horsefield-2019-1 ecoli Escherichia coli (strain K12) x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction experimental particp experimental participant identification experimental particp Horsefield S., Burdett H., Zhang X., Manik MK., Shi Y., Chen J., Qi T., Gilley J., Lai JS., Rank MX., Casey LW., Gu W., Ericsson DJ., Foley G., Hughes RO., Bosanac T., von Itzstein M., Rathjen JP., Nanson JD., Boden M., Dry IB., Williams SJ., Staskawicz BJ., Coleman MP., Ve T., Dodds PN., Kobe B. b.kobe@uq.edu.au Science (New York, N.Y.) 2019 imex curation Only protein-protein interactions imex curation horsefield-2019-3 ecoli Escherichia coli (strain K12) saxs x ray scattering saxs experimental particp experimental participant identification experimental particp Horsefield S., Burdett H., Zhang X., Manik MK., Shi Y., Chen J., Qi T., Gilley J., Lai JS., Rank MX., Casey LW., Gu W., Ericsson DJ., Foley G., Hughes RO., Bosanac T., von Itzstein M., Rathjen JP., Nanson JD., Boden M., Dry IB., Williams SJ., Staskawicz BJ., Coleman MP., Ve T., Dodds PN., Kobe B. b.kobe@uq.edu.au Science (New York, N.Y.) 2019 imex curation Only protein-protein interactions imex curation q6szw1-1 NAD(+) hydrolase SARM1 SARM1 KIAA0524 SAMD2 SARM NADP(+) hydrolase SARM1 Sterile alpha and TIR motif-containing protein 1 Sterile alpha and Armadillo repeat protein Sterile alpha motif domain-containing protein 2 Tir-1 homolog protein protein human Homo sapiens Human MVLTLLLSAYKLCRFFAMSGPRPGAERLAVPGPDGGGGTGPWWAAGGRGPREVSPGAGTEVQDALERALPELQQALSALKQAGGARAVGAGLAEVFQLVEEAWLLPAVGREVAQGLCDAIRLDGGLDLLLRLLQAPELETRVQAARLLEQILVAENRDRVARIGLGVILNLAKEREPVELARSVAGILEHMFKHSEETCQRLVAAGGLDAVLYWCRRTDPALLRHCALALGNCALHGGQAVQRRMVEKRAAEWLFPLAFSKEDELLRLHACLAVAVLATNKEVEREVERSGTLALVEPLVASLDPGRFARCLVDASDTSQGRGPDDLQRLVPLLDSNRLEAQCIGAFYLCAEAAIKSLQGKTKVFSDIGAIQSLKRLVSYSTNGTKSALAKRALRLLGEEVPRPILPSVPSWKEAEVQTWLQQIGFSKYCESFREQQVDGDLLLRLTEEELQTDLGMKSGITRKRFFRELTELKTFANYSTCDRSNLADWLGSLDPRFRQYTYGLVSCGLDRSLLHRVSEQQLLEDCGIHLGVHRARILTAAREMLHSPLPCTGGKPSGDTPDVFISYRRNSGSQLASLLKVHLQLHGFSVFIDVEKLEAGKFEDKLIQSVMGARNFVLVLSPGALDKCMQDHDCKDWVHKEIVTALSCGKNIVPIIDGFEWPEPQVLPEDMQAVLTFNGIKWSHEYQEATIEKIIRFLQGRSSRDSSAGSDTSLEGAAPMGPT q6pds3-1 NAD(+) hydrolase SARM1 NADP(+) hydrolase SARM1 Sterile alpha and TIR motif-containing protein 1 Sarm1 Kiaa0524 protein protein mouse Mus musculus Mouse MVLTLLFSAYKLCRFFTMSGPRPGADRLTVPGPDRSGGASPWWAAGGRGSREVSPGVGTEVQGALERSLPELQQALSELKQASAARAVGAGLAEVFQLVEEAWLLPAVGREVAQGLCDAIRLDGGLDLLLRLLQAPELETRVQAARLLEQILVAENRDRVARIGLGVILNLAKEREPVELARSVAGILEHMFKHSEETCQRLVAAGGLDAVLYWCRRTDPALLRHCALALANCALHGGQTVQRCMVEKRAAEWLFPLAFSKEDELLRLHACLAVAVLATNKEVEREVEHSGTLALVEPLVASLDPGRFARCLVDASDTSQGRGPDDLQSLVLLLDSSRLEAQCIGAFYLCAEAAIKSLQGKTKVFSDIGAIQSLKRLVSYSTNGTTSALAKRALRLLGEEVPRRILPCVASWKEAEVQTWLQQIGFSQYCENFREQQVDGDLLLRLTDEELQTDLGMKSSITRKRFFRELTELKTFASYATCDRSNLADWLGSLDPRFRQYTYGLVSCGLDRSLLHRVSEQQLLEDCGIRLGVHRTRILSAAREMLHSPLPCTGGKLSGDTPDVFISYRRNSGSQLASLLKVHLQLHGFSVFIDVEKLEAGKFEDKLIQSVIAARNFVLVLSAGALDKCMQDHDCKDWVHKEIVTALSCGKNIVPIIDGFEWPEPQALPEDMQAVLTFNGIKWSHEYQEATIEKIIRFLQGRPSQDSSAGSDTSLEGATPMGLP sarm1-6 3596541 N/A 3596544 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified Q6SZW1-1:p.[Leu442Arg;Ile461Asp;Leu514Asp;Leu531Asp;Val533Asp] mSARM1 tSAM(5Mut) mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Binding region Binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Q6SZW1-1:p.Leu473Phe L473F mutation mutation certain certain sequence position certain certain certain sequence position certain false His tag His tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) Stoichiometry: 8.0 direct interaction direct interaction false false false Fig 3A, S11 sarm1-8 3596541 N/A 3596545 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified Q6PDS3-1:p.[Leu442Arg;Ile461Asp;Leu514Asp;Leu531Asp;Val533Asp] mSARM1 tSAM(5Mut) mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3596545 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified Q6PDS3-1:p.[Leu442Arg;Ile461Asp;Leu514Asp;Leu531Asp;Val533Asp] mSARM1 tSAM(5Mut) mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig S16C sarm1-1 3596542 N/A 3596544 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified His tag His tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Binding region Binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Q6SZW1-1:p.Leu473Phe L473F mutation mutation certain certain sequence position certain certain certain sequence position certain false Q6SZW1-1:p.[Leu446Met;Leu505Met;Leu523Met] L446M, L505M, L523M mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 8.0 direct interaction direct interaction false false false Fig 1B. Table S1 sarm1-4 3596543 N/A 3596544 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified Binding region Binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Q6SZW1-1:p.Leu473Phe L473F mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 8.0 direct interaction direct interaction false false false Fig S11