IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ paslawski-2019-2 in vitro In vitro elisa enzyme linked immunosorbent assay ELISA ELISA elisa predetermined predetermined participant predetermined Paslawski W., Zareba-Paslawska J., Zhang X., Hölzl K., Wadensten H., Shariatgorji M., Janelidze S., Hansson O., Forsgren L., Andrén PE., Svenningsson P., Paslawski W., Zareba-Paslawska J., Zhang X., Hölzl K., Wadensten H., Shariatgorji M., Janelidze S., Hansson O., Forsgren L., Andrén PE., Svenningsson P. Parkinsons - Interactions investigated in the context of Parkinsons disease Per.Svenningsson@ki.se Proceedings of the National Academy of Sciences of the United States of America 2019 Only protein-protein interactions imex curation imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. paslawski-2019-4 human-csf Human Cerebrospinal fluid (CSF) csf Cerebrospinal fluid tem transmission electron microscopy tem antibody detection identification by antibody antibody detection Paslawski W., Zareba-Paslawska J., Zhang X., Hölzl K., Wadensten H., Shariatgorji M., Janelidze S., Hansson O., Forsgren L., Andrén PE., Svenningsson P., Paslawski W., Zareba-Paslawska J., Zhang X., Hölzl K., Wadensten H., Shariatgorji M., Janelidze S., Hansson O., Forsgren L., Andrén PE., Svenningsson P. Parkinsons - Interactions investigated in the context of Parkinsons disease immunogold labeling Per.Svenningsson@ki.se Proceedings of the National Academy of Sciences of the United States of America 2019 Only protein-protein interactions imex curation imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. paslawski-2019-1 human-csf Human Cerebrospinal fluid (CSF) csf Cerebrospinal fluid anti bait coip anti bait coimmunoprecipitation anti bait coip sequence tag sequence tag identification sequence tag Paslawski W., Zareba-Paslawska J., Zhang X., Hölzl K., Wadensten H., Shariatgorji M., Janelidze S., Hansson O., Forsgren L., Andrén PE., Svenningsson P., Paslawski W., Zareba-Paslawska J., Zhang X., Hölzl K., Wadensten H., Shariatgorji M., Janelidze S., Hansson O., Forsgren L., Andrén PE., Svenningsson P. Parkinsons - Interactions investigated in the context of Parkinsons disease Some proteins found in the IP have not been entered here;notably Bovine serum albumin and trypsin. Per.Svenningsson@ki.se Proceedings of the National Academy of Sciences of the United States of America 2019 Only protein-protein interactions imex curation imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. paslawski-2019-3 in vitro In vitro aggregation aggregation assay aggregation predetermined predetermined participant predetermined Paslawski W., Zareba-Paslawska J., Zhang X., Hölzl K., Wadensten H., Shariatgorji M., Janelidze S., Hansson O., Forsgren L., Andrén PE., Svenningsson P., Paslawski W., Zareba-Paslawska J., Zhang X., Hölzl K., Wadensten H., Shariatgorji M., Janelidze S., Hansson O., Forsgren L., Andrén PE., Svenningsson P. Parkinsons - Interactions investigated in the context of Parkinsons disease Aggregate formation was monitored by Thioflavin T (ThT) fluorescence. ThT exhibits a fluorescence emission maximum at ~488nm exclusively when bound to amyloid fibrils or amyloidogenic proteins e.g. alpha-synuclein. Per.Svenningsson@ki.se Proceedings of the National Academy of Sciences of the United States of America 2019 Only protein-protein interactions imex curation imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. paslawski-2019-5 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Paslawski W., Zareba-Paslawska J., Zhang X., Hölzl K., Wadensten H., Shariatgorji M., Janelidze S., Hansson O., Forsgren L., Andrén PE., Svenningsson P., Paslawski W., Zareba-Paslawska J., Zhang X., Hölzl K., Wadensten H., Shariatgorji M., Janelidze S., Hansson O., Forsgren L., Andrén PE., Svenningsson P. Parkinsons - Interactions investigated in the context of Parkinsons disease Per.Svenningsson@ki.se Proceedings of the National Academy of Sciences of the United States of America 2019 Only protein-protein interactions imex curation imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. apoe_human Apolipoprotein E APOE protein protein human Homo sapiens Human MKVLWAALLVTFLAGCQAKVEQAVETEPEPELRQQTEWQSGQRWELALGRFWDYLRWVQTLSEQVQEELLSSQVTQELRALMDETMKELKAYKSELEEQLTPVAEETRARLSKELQAAQARLGADMEDVCGRLVQYRGEVQAMLGQSTEELRVRLASHLRKLRKRLLRDADDLQKRLAVYQAGAREGAERGLSAIRERLGPLVEQGRVRAATVGSLAGQPLQERAQAWGERLRARMEEMGSRTRDRLDEVKEQVAEVRAKLEEQAQQIRLQAEAFQARLKSWFEPLVEDMQRQWAGLVEKVQAAVGTSAAPVPSDNH syua_human Alpha-synuclein Non-A beta component of AD amyloid Non-A4 component of amyloid precursor SNCA NACP PARK1 protein protein human Homo sapiens Human MDVFMKGLSKAKEGVVAAAEKTKQGVAEAAGKTKEGVLYVGSKTKEGVVHGVATVAEKTKEQVTNVGGAVVTGVTAVAQKTVEGAGSIAAATGFVKKDQLGKNEEGAPQEGILEDMPVDPDNEAYEMPSEEGYQDYEPEA hv323_human Immunoglobulin heavy variable 3-23 IGHV3-23 Ig heavy chain V-III region VH26 Ig heavy chain V-III region LAY Ig heavy chain V-III region POM Ig heavy chain V-III region TEI Ig heavy chain V-III region TIL Ig heavy chain V-III region TUR Ig heavy chain V-III region WAS Ig heavy chain V-III region ZAP protein protein human Homo sapiens Human MEFGLSWLFLVAILKGVQCEVQLVESGGGLVQPGGSLRLSCAASGFTFSSYAMSWVRQAPGKGLEWVSAISGSGGSTYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAK clus_human Clusterin CLU APOJ CLI KUB1 Complement-associated protein SP-40,40 Complement cytolysis inhibitor NA1/NA2 Apolipoprotein J Testosterone-repressed prostate message 2 Ku70-binding protein 1 Aging-associated gene 4 protein AAG4 Sulfated glycoprotein 2 protein protein human Homo sapiens Human MMKTLLLFVGLLLTWESGQVLGDQTVSDNELQEMSNQGSKYVNKEIQNAVNGVKQIKTLIEKTNEERKTLLSNLEEAKKKKEDALNETRESETKLKELPGVCNETMMALWEECKPCLKQTCMKFYARVCRSGSGLVGRQLEEFLNQSSPFYFWMNGDRIDSLLENDRQQTHMLDVMQDHFSRASSIIDELFQDRFFTREPQDTYHYLPFSLPHRRPHFFFPKSRIVRSLMPFSPYEPLNFHAMFQPFLEMIHEAQQAMDIHFHSPAFQHPPTEFIREGDDDRTVCREIRHNSTGCLRMKDQCDKCREILSVDCSTNNPSQAKLRRELDESLQVAERLTRKYNELLKSYQWKMLNTSSLLEQLNEQFNWVSRLANLTQGEDQYYLRVTTVASHTSDSDVPSGVTEVVVKLFDSDPITVTVPVEVSRKNPKFMETVAEKALQEYRKKHREE apoa1_human Apolipoprotein A-I APOA1 Apolipoprotein A1 protein protein human Homo sapiens Human MKAAVLTLAVLFLTGSQARHFWQQDEPPQSPWDRVKDLATVYVDVLKDSGRDYVSQFEGSALGKQLNLKLLDNWDSVTSTFSKLREQLGPVTQEFWDNLEKETEGLRQEMSKDLEEVKAKVQPYLDDFQKKWQEEMELYRQKVEPLRAELQEGARQKLHELQEKLSPLGEEMRDRARAHVDALRTHLAPYSDELRQRLAARLEALKENGGARLAEYHAKATEHLSTLSEKAKPALEDLRQGLLPVLESFKVSFLSALEEYTKKLNTQ apoe-snca-1 3649017 N/A 3649022 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level human-csf Human Cerebrospinal fluid (CSF) csf Cerebrospinal fluid N/A 3649023 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level human-csf Human Cerebrospinal fluid (CSF) csf Cerebrospinal fluid physical association physical association false false false 1B apoe-snca-2 3649017 N/A 3649022 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level human-csf Human Cerebrospinal fluid (CSF) csf Cerebrospinal fluid N/A 3649023 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level human-csf Human Cerebrospinal fluid (CSF) csf Cerebrospinal fluid physical association physical association false false false 1B apoe-snca-4 3649017 N/A 3649022 unspecified role unspecified role bait bait purified purified ecoli Escherichia coli (strain K12) N/A 3649023 unspecified role unspecified role prey prey purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 physical association physical association false false false 2C HDL and VLDL vesicles enriched with aSN and/or ApoE;samples had been passed through a size-exclusion filter prior to Elisa analysis. apoe-snca-5 3649017 N/A 3649022 unspecified role unspecified role prey prey purified purified ecoli Escherichia coli (strain K12) N/A 3649023 unspecified role unspecified role bait bait purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 physical association physical association false false false 2D HDL and VLDL vesicles enriched with aSN and/or ApoE;samples had been passed through a size-exclusion filter prior to Elisa analysis. apoe-snca-3 3649018 N/A 3649022 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3649023 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level colocalization colocalization false false false 1E aSN and ApoE double immune-gold labeling revealed a colocalization of aSN and ApoE on larger (>30 nm) vesicular structures in CSF. snca-snca-24 3649018 N/A 3649023 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3649023 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level colocalization colocalization false false false S14 snca-apoa1-1 3649019 N/A 3649024 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3649025 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3649026 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3649022 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3649023 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level association association false false false Table 1 snca-snca-23 3649020 N/A 3649023 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) N/A 3649023 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 1D After addition of recombinant ApoE no significant changes in the aggregation profile of the recombinant aSN were observed. apoe-snca-6 3649021 N/A 3649022 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level human-plasma homo sapiens blood plasma plasma blood plasma N/A 3649023 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 physical association physical association false false false 2A human plasma VLDL contains APOE but plasma HDL does not.