DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ masiulis-2019-1 human-293s Human 293S cell line Human 293s 293S cell line 3d-em 3D electron microscopy 3D-EM electron tomog experimental particp experimental participant identification experimental particp Masiulis S., Desai R., Uchański T., Serna Martin I., Laverty D., Karia D., Malinauskas T., Zivanov J., Pardon E., Kotecha A., Steyaert J., Miller KW., Aricescu AR. radu@mrc-lmb.cam.ac.uk Nature 2019 imex curation Only protein-protein interactions imex curation p18507-2 Gamma-aminobutyric acid receptor subunit gamma-2 GABRG2 GABA(A) receptor subunit gamma-2 protein protein human Homo sapiens Human MSSPNIWSTGSSVYSTPVFSQKMTVWILLLLSLYPGFTSQKSDDDYEDYASNKTWVLTPKVPEGDVTVILNNLLEGYDNKLRPDIGVKPTLIHTDMYVNSIGPVNAINMEYTIDIFFAQTWYDRRLKFNSTIKVLRLNSNMVGKIWIPDTFFRNSKKADAHWITTPNRMLRIWNDGRVLYTLRLTIDAECQLQLHNFPMDEHSCPLEFSSYGYPREEIVYQWKRSSVEVGDTRSWRLYQFSFVGLRNTTEVVKTTSGDYVVMSVYFDLSRRMGYFTIQTYIPCTLIVVLSWVSFWINKDAVPARTSLGITTVLTMTTLSTIARKSLPKVSYVTAMDLFVSVCFIFVFSALVEYGTLHYFVSNRKPSKDKDKKKKNPLLRMFSFKAPTIDIRPRSATIQMNNATHLQERDEEYGYECLDGKDCASFFCCFEDCRTGAWRHGRIHIRIAKMDSYARIFFPTAFCLFNLVYWVSYLYL p28472-2 Gamma-aminobutyric acid receptor subunit beta-3 GABA(A) receptor subunit beta-3 GABRB3 protein protein human Homo sapiens Human MCSGLLELLLPIWLSWTLGTRGSEPRSVNDPGNMSFVKETVDKLLKGYDIRLRPDFGGPPVCVGMNIDIASIDMVSEVNMDYTLTMYFQQYWRDKRLAYSGIPLNLTLDNRVADQLWVPDTYFLNDKKSFVHGVTVKNRMIRLHPDGTVLYGLRITTTAACMMDLRRYPLDEQNCTLEIESYGYTTDDIEFYWRGGDKAVTGVERIELPQFSIVEHRLVSRNVVFATGAYPRLSLSFRLKRNIGYFILQTYMPSILITILSWVSFWINYDASAARVALGITTVLTMTTINTHLRETLPKIPYVKAIDMYLMGCFVFVFLALLEYAFVNYIFFGRGPQRQKKLAEKTAKAKNDRSKSESNRVDAHGNILLTSLEVHNEMNEVSGGIGDTRNSAISFDNSGIQYRKQSMPREGHGRFLGDRSLPHKKTHLRRRSSQLKIKIPDLTDVNAIDRWSRIVFPFTFSLFNLVYWLYYVN Variant in position: 11:P->S (in ECA5, the mutant protein is hyperglycosylated and has reduced mean current densities compared to wild-type, dbSNP:rs25409). Variant in position: 15:S->F (in ECA5, the mutant protein is hyperglycosylated and has reduced mean current densities compared to wild-type). gbra1_human Gamma-aminobutyric acid receptor subunit alpha-1 GABRA1 GABA(A) receptor subunit alpha-1 protein protein human Homo sapiens Human MRKSPGLSDCLWAWILLLSTLTGRSYGQPSLQDELKDNTTVFTRILDRLLDGYDNRLRPGLGERVTEVKTDIFVTSFGPVSDHDMEYTIDVFFRQSWKDERLKFKGPMTVLRLNNLMASKIWTPDTFFHNGKKSVAHNMTMPNKLLRITEDGTLLYTMRLTVRAECPMHLEDFPMDAHACPLKFGSYAYTRAEVVYEWTREPARSVVVAEDGSRLNQYDLLGQTVDSGIVQSSTGEYVVMTTHFHLKRKIGYFVIQTYLPCIMTVILSQVSFWLNRESVPARTVFGVTTVLTMTTLSISARNSLPKVAYATAMDWFIAVCYAFVFSALIEFATVNYFTKRGYAWDGKSVVPEKPKKVKDPLIKKNNTYAPTATSYTPNLARGDPGLATIAKSATIEPKEVKPETKPPEPKKTFNSVSKIDRLSRIAFPLLFGIFNLVYWATYLNREPQLKAPTPHQ cryo em-1 3542630 N/A 3542631 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified rho tag rho tag rho tag rho tag 1D4 tag rho1D4 tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human-293s Human 293S cell line Human 293s 293S cell line Stoichiometry: 1.0 N/A 3542632 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified human-293s Human 293S cell line Human 293s 293S cell line Stoichiometry: 2.0 N/A 3542633 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified flag tag flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human-293s Human 293S cell line Human 293s 293S cell line Stoichiometry: 2.0 direct interaction direct interaction false false false Fig. 1: Structure of the α1β3γ2L GABAA receptor in complex with picrotoxin