UniProt UniProt http://www.uniprot.org gauba-2019-3 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Gauba E., Chen H., Guo L., Du H., Gauba E., Chen H., Guo L., Du H. heng.du@utdallas.edu Neurobiology of disease 2019 Only protein-protein interactions imex curation imex curation gauba-2019-2 mouse-brain-cortex Mus musculus brain cortex brain_cortex Brain cortex [Cerebral cortex; Cerebrum cortex; Cortex; Pallium] pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Gauba E., Chen H., Guo L., Du H., Gauba E., Chen H., Guo L., Du H. heng.du@utdallas.edu Neurobiology of disease 2019 Only protein-protein interactions imex curation imex curation gauba-2019-1 human-brain Homo sapiens brain brain Brain [cerebrum] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gauba E., Chen H., Guo L., Du H., Gauba E., Chen H., Guo L., Du H. heng.du@utdallas.edu Neurobiology of disease 2019 Only protein-protein interactions imex curation imex curation gauba-2019-4 mouse-brain-cortex Mus musculus brain cortex brain_cortex Brain cortex [Cerebral cortex; Cerebrum cortex; Cortex; Pallium] fluorescence imaging fluorescence microscopy fluorescence imaging immunohistochemistry immunohistochemistry Gauba E., Chen H., Guo L., Du H., Gauba E., Chen H., Guo L., Du H. heng.du@utdallas.edu Neurobiology of disease 2019 Only protein-protein interactions imex curation imex curation ppid_human Peptidyl-prolyl cis-trans isomerase D Cyclophilin-40 Cyclophilin-related protein PPID CYP40 CYPD Rotamase D 40 kDa peptidyl-prolyl cis-trans isomerase protein protein human Homo sapiens Human MSHPSPQAKPSNPSNPRVFFDVDIGGERVGRIVLELFADIVPKTAENFRALCTGEKGIGHTTGKPLHFKGCPFHRIIKKFMIQGGDFSNQNGTGGESIYGEKFEDENFHYKHDREGLLSMANAGRNTNGSQFFITTVPTPHLDGKHVVFGQVIKGIGVARILENVEVKGEKPAKLCVIAECGELKEGDDGGIFPKDGSGDSHPDFPEDADIDLKDVDKILLITEDLKNIGNTFFKSQNWEMAIKKYAEVLRYVDSSKAVIETADRAKLQPIALSCVLNIGACKLKMSNWQGAIDSCLEALELDPSNTKALYRRAQGWQGLKEYDQALADLKKAQGIAPEDKAIQAELLKVKQKIKAQKDKEKAVYAKMFA atpo_human ATP synthase subunit O, mitochondrial ATP5PO ATP synthase peripheral stalk subunit OSCP ATP5O ATPO Oligomycin sensitivity conferral protein protein protein human Homo sapiens Human MAAPAVSGLSRQVRCFSTSVVRPFAKLVRPPVQVYGIEGRYATALYSAASKQNKLEQVEKELLRVAQILKEPKVAASVLNPYVKRSIKVKSLNDITAKERFSPLTTNLINLLAENGRLSNTQGVVSAFSTMMSVHRGEVPCTVTSASPLEEATLSELKTVLKSFLSQGQVLKLEAKTDPSILGGMIVRIGEKYVDMSVKTKIQKLGRAMREIV p05067-pro_0000000092 Amyloid-beta protein 42 Alzheimer disease amyloid protein Cerebral vascular amyloid peptide APP A4 AD1 ABPP PreA4 Protease nexin-II APPI Amyloid precursor protein Amyloid-beta A4 protein Alzheimer disease amyloid A4 protein homolog Amyloid-beta (A4) precursor protein protein protein human Homo sapiens Human DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA 672 713 ppid_mouse Peptidyl-prolyl cis-trans isomerase D Ppid Rotamase D 40 kDa peptidyl-prolyl cis-trans isomerase Cyclophilin-40 protein protein mouse Mus musculus Mouse MSHASPAAKPSNSKNPRVFFDVDIGGERVGRIVLELFADIVPKTAENFRALCTGEKGTGSTTGKPLHFKGCPFHRIIKKFMIQGGDFSNQNGTGGESIYGEKFEDENFHYKHDREGLLSMANAGPNTNGSQFFITTVPTPHLDGKHVVFGQVIKGLGVARTLENVEVNGEKPAKLCVIAECGELKEGDDWGIFPKDGSGDSHPDFPEDADIDLKDVDKILLISEDLKNIGNTFFKSQNWEMAIKKYAKVLRYVDSSKAVIEKADRSRLQPIALSCVLNIGACKLKMSNWQGAIDSCLEALEMDPSNTKALYRKAQGWQGLKEYDQALADLKKAQEIAPGDKAIQAELLKVKQMIKAQKDKEKAVYAKMFA mint PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides atpo_mouse ATP synthase subunit O, mitochondrial Atp5po ATP synthase peripheral stalk subunit OSCP Atp5o D12Wsu28e Oligomycin sensitivity conferral protein protein protein mouse Mus musculus Mouse MAAPAASGLSRQVRSFSTSVVRPFAKLVRPPVQVYGIEGRYATALYSAASKEKKLDQVEKELLRVGQLLKDPKVSLAVLNPYIKRTVKVKSLNDITKREKFSPLTANLMNLLAENGRLGNTQGIISAFSTIMSVHRGEVPCTVTTASPLDDAVLSELKTVLKSFLSPNQILKLEIKTDPSIMGGMIVRIGEKYVDMSAKSKIQKLSKAMREML p12023-pro_0000000114 Amyloid-beta precursor protein App ABPP Alzheimer disease amyloid A4 protein homolog Amyloid precursor protein Amyloidogenic glycoprotein A4 AD1 Alzheimer disease amyloid protein Amyloid-beta (A4) precursor protein Amyloid-beta A4 protein protein protein mouse Mus musculus Mouse LEVPTDGNAGLLAEPQIAMFCGKLNMHMNVQNGKWESDPSGTKTCIGTKEGILQYCQEVYPELQITNVVEANQPVTIQNWCKRGRKQCKTHTHIVIPYRCLVGEFVSDALLVPDKCKFLHQERMDVCETHLHWHTVAKETCSEKSTNLHDYGMLLPCGIDKFRGVEFVCCPLAEESDSVDSADAEEDDSDVWWGGADTDYADGGEDKVVEVAEEEEVADVEEEEADDDEDVEDGDEVEEEAEEPYEEATERTTSTATTTTTTTESVEEVVREVCSEQAETGPCRAMISRWYFDVTEGKCVPFFYGGCGGNRNNFDTEEYCMAVCGSVSTQSLLKTTSEPLPQDPDKLPTTAASTPDAVDKYLETPGDENEHAHFQKAKERLEAKHRERMSQVMREWEEAERQAKNLPKADKKAVIQHFQEKVESLEQEAANERQQLVETHMARVEAMLNDRRRLALENYITALQAVPPRPHHVFNMLKKYVRAEQKDRQHTLKHFEHVRMVDPKKAAQIRSQVMTHLRVIYERMNQSLSLLYNVPAVAEEIQDEVDELLQKEQNYSDDVLANMISEPRISYGNDALMPSLTETKTTVELLPVNGEFSLDDLQPWHPFGVDSVPANTENEVEPVDARPAADRGLTTRPGSGLTNIKTEEISEVKMDAEFGHDSGFEVRHQKLVFFAEDVGSNKGAIIGLMVGGVVIATVIVITLVMLKKKQYTSIHHGVVEVDAAVTPEERHLSKMQQNGYENPTYKFFEQMQN 18 770 atp5po-ppid-3 3542945 N/A 3542949 unspecified role unspecified role prey prey purified purified ecoli Escherichia coli (strain K12) N/A 3542950 unspecified role unspecified role bait bait purified purified n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 3542945 Fig. 1E Amyloid-beta protein 42 (P05067-PRO_0000000092) atp5po-ppid-4 3542945 N/A 3542951 unspecified role unspecified role prey prey chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3542949 unspecified role unspecified role prey prey purified purified ecoli Escherichia coli (strain K12) N/A 3542950 unspecified role unspecified role bait bait purified purified n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig. 4A Amyloid-beta protein 42 (P05067-PRO_0000000092) The the KD of OSCP/Amyloid-beta 43 interaction was dramatically decreased in the presence of CypD atp5po-app-1 3542945 N/A 3542951 unspecified role unspecified role prey prey chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3542950 unspecified role unspecified role bait bait purified purified n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 3542945 Fig. 4A The the KD of OSCP/Amyloid-beta 43 interaction was dramatically decreased in the presence of CypD atp5po-ppid-2 3542946 N/A 3542952 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3542953 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level proximity proximity close-contact colocalization neighbourhood interaction false false false Fig. 1B Mice exhibited substantially increased density of Ppid/Atp5po complexes in their neocortex in an age-dependent manner app-atp5po-1 3542946 N/A 3542954 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3542953 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level proximity proximity close-contact colocalization neighbourhood interaction false false false Fig. 4B Mice exhibited substantially increased density of amyloid beta 42/ATP5PO complexes in their neocortex in an age-dependent manner and a significant decrease in complex density in Ppid−/− mice atp5po-ppid-1 3542947 N/A 3542949 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3542950 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false Fig. 1A Brains from Alzheimer's Disease patients showed significantly increased levels of interaction over non-demented controls. atp5po-ppid-5 3542948 N/A 3542952 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3542953 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level colocalization colocalization false false false Fig. 1C