IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ pasquin-2018-2 in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip tag visualisation tag visualisation Pasquin S., Chehboun S., Dejda A., Meliani Y., Savin V., Warner GJ., Bosse R., Tormo A., Mayer G., Sharma M., Sapieha P., Martel C., Gauchat JF. Interactions involving recombinant CLCF1 with a tag (his/biotin/flag) have not been annotated as the publication does not specify when murine or human recombinant proteins were used. The authors did not reply to a request for clarification. jf.gauchat@umontreal.ca Scientific reports 2018 Only protein-protein interactions imex curation imex curation pasquin-2018-3 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column experimental particp experimental participant identification experimental particp Pasquin S., Chehboun S., Dejda A., Meliani Y., Savin V., Warner GJ., Bosse R., Tormo A., Mayer G., Sharma M., Sapieha P., Martel C., Gauchat JF. Interactions involving recombinant CLCF1 with a tag (his/biotin/flag) have not been annotated as the publication does not specify when murine or human recombinant proteins were used. The authors did not reply to a request for clarification. CLCF1 was mixed with mouse or human serum and Fast Performance Liquid Chromatography (FPLC) performed using a Superose 6 column. jf.gauchat@umontreal.ca Scientific reports 2018 Only protein-protein interactions imex curation imex curation pasquin-2018-1 human-293 flp-in Homo sapiens 293 flp-in 293 flp-in anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Pasquin S., Chehboun S., Dejda A., Meliani Y., Savin V., Warner GJ., Bosse R., Tormo A., Mayer G., Sharma M., Sapieha P., Martel C., Gauchat JF. Interactions involving recombinant CLCF1 with a tag (his/biotin/flag) have not been annotated as the publication does not specify when murine or human recombinant proteins were used. The authors did not reply to a request for clarification. The Apoe4 variant has a C130R mutation and Apoe2 has an R176C mutation. (Uniprot VAR_000652 and VAR_000664 respectively.) Transfectants used here are assumed to be of Human origin based on Methods section "Generation of transfectants co-expressing CLCF1 and ApoE". jf.gauchat@umontreal.ca Scientific reports 2018 Only protein-protein interactions imex curation imex curation clcf1_mouse Cardiotrophin-like cytokine factor 1 Clcf1 Bsf3 Nnt1 B-cell-stimulating factor 3 Novel neurotrophin-1 protein protein mouse Mus musculus Mouse MDLRAGDSWGMLACLCTVLWHLPAVPALNRTGDPGPGPSIQKTYDLTRYLEHQLRSLAGTYLNYLGPPFNEPDFNPPRLGAETLPRATVNLEVWRSLNDRLRLTQNYEAYSHLLCYLRGLNRQAATAELRRSLAHFCTSLQGLLGSIAGVMATLGYPLPQPLPGTEPAWAPGPAHSDFLQKMDDFWLLKELQTWLWRSAKDFNRLKKKMQPPAASVTLHLEAHGF apoe_mouse Apolipoprotein E Apoe protein protein mouse Mus musculus Mouse MKALWAVLLVTLLTGCLAEGEPEVTDQLEWQSNQPWEQALNRFWDYLRWVQTLSDQVQEELQSSQVTQELTALMEDTMTEVKAYKKELEEQLGPVAEETRARLGKEVQAAQARLGADMEDLRNRLGQYRNEVHTMLGQSTEEIRARLSTHLRKMRKRLMRDAEDLQKRLAVYKAGAREGAERGVSAIRERLGPLVEQGRQRTANLGAGAAQPLRDRAQAFGDRIRGRLEEVGNQARDRLEEVREHMEEVRSKMEEQTQQIRLQAEIFQARLKGWFEPIVEDMHRQWANLMEKIQASVATNPIITPVAQENQ clcf1_human Cardiotrophin-like cytokine factor 1 CLCF1 BSF3 CLC NNT1 B-cell-stimulating factor 3 Novel neurotrophin-1 protein protein human Homo sapiens Human MDLRAGDSWGMLACLCTVLWHLPAVPALNRTGDPGPGPSIQKTYDLTRYLEHQLRSLAGTYLNYLGPPFNEPDFNPPRLGAETLPRATVDLEVWRSLNDKLRLTQNYEAYSHLLCYLRGLNRQAATAELRRSLAHFCTSLQGLLGSIAGVMAALGYPLPQPLPGTEPTWTPGPAHSDFLQKMDDFWLLKELQTWLWRSAKDFNRLKKKMQPPAAAVTLHLGAHGF apoe_human Apolipoprotein E APOE protein protein human Homo sapiens Human MKVLWAALLVTFLAGCQAKVEQAVETEPEPELRQQTEWQSGQRWELALGRFWDYLRWVQTLSEQVQEELLSSQVTQELRALMDETMKELKAYKSELEEQLTPVAEETRARLSKELQAAQARLGADMEDVCGRLVQYRGEVQAMLGQSTEELRVRLASHLRKLRKRLLRDADDLQKRLAVYQAGAREGAERGLSAIRERLGPLVEQGRVRAATVGSLAGQPLQERAQAWGERLRARMEEMGSRTRDRLDEVKEQVAEVRAKLEEQAQQIRLQAEAFQARLKSWFEPLVEDMQRQWAGLVEKVQAAVGTSAAPVPSDNH apoe-1 3649171 N/A 3649174 unspecified role unspecified role prey prey purified purified C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 3649175 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level mouse-serum Mouse serum serum Serum physical association physical association false false false 1C apoe-clcf1-2 3649171 N/A 3649176 unspecified role unspecified role prey prey purified purified C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 3649177 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level human-serum Homo sapiens serum Serum physical association physical association false false false 1D apoe-clcf1-3 3649172 N/A 3649174 tag visualisation tag visualisation unspecified role unspecified role neutral component neutral component purified purified C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 3649175 western blot western blot Immuno blot Immuno blot unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level mouse-serum Mouse serum serum Serum physical association physical association false false false 4A/C apoe-clcf1-4 3649172 N/A 3649176 tag visualisation tag visualisation unspecified role unspecified role neutral component neutral component purified purified C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 3649177 western blot western blot Immuno blot Immuno blot unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level human-serum Homo sapiens serum Serum physical association physical association false false false 4B apoe-clcf1-1 3649173 N/A 3649176 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 3649177 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed P02649:p.Cys130Arg mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false P02649:p.Arg176Cys mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 1B