DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ yan-2018-3 ecoli Escherichia coli (strain K12) molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column experimental particp experimental participant identification experimental particp Yan L., Qi Y., Huang X., Yu C., Lan L., Guo X., Rao Z., Hu J., Lou Z. huj@ibp.ac.cn; louzy@mail.tsinghua.edu.cn Nature structural & molecular biology 2018 imex curation Only protein-protein interactions imex curation yan-2018-2 ecoli Escherichia coli (strain K12) x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction experimental particp experimental participant identification experimental particp Yan L., Qi Y., Huang X., Yu C., Lan L., Guo X., Rao Z., Hu J., Lou Z. huj@ibp.ac.cn; louzy@mail.tsinghua.edu.cn Nature structural & molecular biology 2018 imex curation Only protein-protein interactions imex curation yan-2018-4 ecoli Escherichia coli (strain K12) fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret experimental particp experimental participant identification experimental particp Yan L., Qi Y., Huang X., Yu C., Lan L., Guo X., Rao Z., Hu J., Lou Z. huj@ibp.ac.cn; louzy@mail.tsinghua.edu.cn Nature structural & molecular biology 2018 imex curation Only protein-protein interactions imex curation mfn1_human Mitofusin-1 Transmembrane GTPase MFN1 Fzo homolog MFN1 protein protein human Homo sapiens Human MAEPVSPLKHFVLAKKAITAIFDQLLEFVTEGSHFVEATYKNPELDRIATEDDLVEMQGYKDKLSIIGEVLSRRHMKVAFFGRTSSGKSSVINAMLWDKVLPSGIGHITNCFLSVEGTDGDKAYLMTEGSDEKKSVKTVNQLAHALHMDKDLKAGCLVRVFWPKAKCALLRDDLVLVDSPGTDVTTELDSWIDKFCLDADVFVLVANSESTLMNTEKHFFHKVNERLSKPNIFILNNRWDASASEPEYMEDVRRQHMERCLHFLVEELKVVNALEAQNRIFFVSAKEVLSARKQKAQGMPESGVALAEGFHARLQEFQNFEQIFEECISQSAVKTKFEQHTIRAKQILATVKNIMDSVNLAAEDKRHYSVEEREDQIDRLDFIRNQMNLLTLDVKKKIKEVTEEVANKVSCAMTDEICRLSVLVDEFCSEFHPNPDVLKIYKSELNKHIEDGMGRNLADRCTDEVNALVLQTQQEIIENLKPLLPAGIQDKLHTLIPCKKFDLSYNLNYHKLCSDFQEDIVFRFSLGWSSLVHRFLGPRNAQRVLLGLSEPIFQLPRSLASTPTAPTTPATPDNASQEELMITLVTGLASVTSRTSMGIIIVGGVIWKTIGWKLLSVSLTMYGALYLYERLSWTTHAKERAFKQQFVNYATEKLRMIVSSTSANCSHQVKQQIATTFARLCQQVDITQKQLEEEIARLPKEIDQLEKIQNNSKLLRNKAVQLENELENFTKQFLPSSNEES mfn1-10 3545468 N/A 3545471 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false p.(Lys365_Glu693delinsGlySerGlySerGlySerGlyGlySer) GSGSGSGGS mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Fig 3b, Supplementary Fig.4c mfn1-16 3545468 N/A 3545471 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false p.(Lys365_Glu693delinsGlySerGlySerGlySerGlyGlySer) GSGSGSGGS mutation mutation certain certain sequence position certain certain certain sequence position certain false Q8IWA4:p.Arg238Ala R238A mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false Q8IWA4:p.Ser85Ala S85A mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Supplementary Fig. 4e and f mfn1-17 3545468 N/A 3545471 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified p.(Lys365_Glu693delinsGlySerGlySerGlySerGlyGlySer) GSGSGSGGS mutation mutation certain certain sequence position certain certain certain sequence position certain false his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q8IWA4:p.Glu209Ala E209A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false Q8IWA4:p.His147Ala H147A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false Q8IWA4:p.Lys150Ala R150A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false Q8IWA4:p.Glu245Ala E245A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false Q8IWA4:p.Tyr248Ala Y248A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false Q8IWA4:p.Ile105Ala I105A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Supplementary Fig. 4e mfn1-18 3545468 N/A 3545471 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false p.(Lys365_Glu693delinsGlySerGlySerGlySerGlyGlySer) GSGSGSGGS mutation mutation certain certain sequence position certain certain certain sequence position certain false Q8IWA4:p.Gly106Asp G106D mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false Q8IWA4:p.Gln255Arg Q255R mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Fig .5b mfn1-19 3545468 N/A 3545471 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false p.(Lys365_Glu693delinsGlySerGlySerGlySerGlyGlySer) GSGSGSGGS mutation mutation certain certain sequence position certain certain certain sequence position certain false Q8IWA4:p.Lys229Gln K229Q mutation mutation certain certain sequence position certain certain certain sequence position certain false Q8IWA4:p.Asp198Asn D198N mutation mutation certain certain sequence position certain certain certain sequence position certain false Q8IWA4:p.Lys229Trp K229W mutation mutation certain certain sequence position certain certain certain sequence position certain false Q8IWA4:p.Arg259His R259H mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Fig .5b and c mfn1-20 3545468 N/A 3545471 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false p.(Lys365_Glu693delinsGlySerGlySerGlySerGlyGlySer) GSGSGSGGS mutation mutation certain certain sequence position certain certain certain sequence position certain false Q8IWA4:p.[Arg73Gln;Asp198Asn] R73Q,D198N mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Fig. 5c mfn1-21 3545468 N/A 3545471 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false p.(Lys365_Glu693delinsGlySerGlySerGlySerGlyGlySer) GSGSGSGGS mutation mutation certain certain sequence position certain certain certain sequence position certain false Q8IWA4:p.Ser86Ala S86A mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Supplementary Fig. 4F mfn1-22 3545468 N/A 3545471 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false p.(Lys365_Glu693delinsGlySerGlySerGlySerGlyGlySer) GSGSGSGGS mutation mutation certain certain sequence position certain certain certain sequence position certain false Q8IWA4:p.Arg73Asp R73D mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Fig. 4c mfn1-23 3545468 N/A 3545471 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false p.(Lys365_Glu693delinsGlySerGlySerGlySerGlyGlySer) GSGSGSGGS mutation mutation certain certain sequence position certain certain certain sequence position certain false Q8IWA4:p.Asp198Arg D198R mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Fig. 4c mfn1-24 3545468 N/A 3545471 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false p.(Lys365_Glu693delinsGlySerGlySerGlySerGlyGlySer) GSGSGSGGS mutation mutation certain certain sequence position certain certain certain sequence position certain false Q8IWA4:p.[Arg73Asp;Asp198Arg] R73D,D198R mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Fig. 4c mfn1-25 3545468 N/A 3545471 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false p.(Lys365_Glu693delinsGlySerGlySerGlySerGlyGlySer) GSGSGSGGS mutation mutation certain certain sequence position certain certain certain sequence position certain false Q8IWA4:p.Arg73Gln R73Q mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false Q8IWA4:p.Val252Gly V252G mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Fig. 5b and c mfn1-1 3545469 N/A 3545471 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false p.(Lys365_Glu693delinsGlySerGlySerGlySerGlyGlySer) GSGSGSGGS mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Fig. 1, Table 1 mfn1-15 3545470 N/A 3545471 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role fluorescence donor fluorescence donor purified purified his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false p.(Lys365_Glu693delinsGlySerGlySerGlySerGlyGlySer) GSGSGSGGS mutation mutation certain certain sequence position certain certain certain sequence position certain false cy5 label cy5 label cy5 label cy5 label n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 N/A 3545471 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept purified purified his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false p.(Lys365_Glu693delinsGlySerGlySerGlySerGlyGlySer) GSGSGSGGS mutation mutation certain certain sequence position certain certain certain sequence position certain false cy3 label cy3 label cy3 label cy3 label n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Fig. 6, Supplementary Figure 6