IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ wohlfarth-2017-2 human-colo_320 Human colon adenocarcinoma colo_320 Human colorectal adenocarcinoma Colo320 mirna interference luciferase assay miRNA interference luciferase reporter assay miRNA interference luciferase assay tag luciferase tag visualisation by luciferase assay tag luciferase Wohlfarth C., Schmitteckert S., Härtle JD., Houghton LA., Dweep H., Fortea M., Assadi G., Braun A., Mederer T., Pöhner S., Becker PP., Fischer C., Granzow M., Mönnikes H., Mayer EA., Sayuk G., Boeckxstaens G., Wouters MM., Simrén M., Lindberg G., Ohlsson B., Schmidt PT., Dlugosz A., Agreus L., Andreasson A., D'Amato M., Burwinkel B., Bermejo JL., Röth R., Lasitschka F., Vicario M., Metzger M., Santos J., Rappold GA., Martinez C., Niesler B., Wohlfarth C., Schmitteckert S., Härtle JD., Houghton LA., Dweep H., Fortea M., Assadi G., Braun A., Mederer T., Pöhner S., Becker PP., Fischer C., Granzow M., Mönnikes H., Mayer EA., Sayuk G., Boeckxstaens G., Wouters MM., Simrén M., Lindberg G., Ohlsson B., Schmidt PT., Dlugosz A., Agreus L., Andreasson A., D'Amato M., Burwinkel B., Bermejo JL., Röth R., Lasitschka F., Vicario M., Metzger M., Santos J., Rappold GA., Martinez C., Niesler B. miR 15b/16/497 were also shown to downregulate HTR4 mRNA, but in the experiment in fig 6 it is not possible to discerne if all the 3 bind or which of them do. beate.niesler@med.uni-heidelberg.de Scientific reports 2017 imex curation Only protein-protein interactions imex curation mrna_htr4 5-hydroxytryptamine receptor 4 mrna messenger rna mRNA mrna human Homo sapiens Human GACCCTGTGGGCATTGAAATCCAACTCACTCATGCTTATTTCCTGTAATGGACAAACTTGATGCTAATGTGAGTTCTGAGGAGGGTTTCGGGTCAGTGGAGAAGGTGGTGCTGCTCACGTTTCTCTCGACGGTTATCCTGATGGCCATCTTGGGGAACCTGCTGGTGATGGTGGCTGTGTGCTGGGACAGGCAGCTCAGGAAAATAAAAACAAATTATTTCATTGTATCTCTTGCTTTTGCGGATCTGCTGGTTTCGGTGCTGGTGATGCCCTTTGGTGCCATTGAGCTGGTTCAAGACATCTGGATTTATGGGGAGGTGTTTTGTCTTGTTCGGACATCTCTGGACGTCCTGCTCACAACGGCATCGATTTTTCACCTGTGCTGCATTTCTCTGGATAGGTATTACGCCATCTGCTGCCAGCCTTTGGTCTATAGGAACAAGATGACCCCTCTGCGCATCGCATTAATGCTGGGAGGCTGCTGGGTCATCCCCACGTTTATTTCTTTTCTCCCTATAATGCAAGGCTGGAATAACATTGGCATAATTGATTTGATAGAAAAGAGGAAGTTCAACCAGAACTCTAACTCTACGTACTGTGTCTTCATGGTCAACAAGCCCTACGCCATCACCTGCTCTGTGGTGGCCTTCTACATCCCATTTCTCCTCATGGTGCTGGCCTATTACCGCATCTATGTCACAGCTAAGGAGCATGCCCATCAGATCCAGATGTTACAACGGGCAGGAGCCTCCTCCGAGAGCAGGCCTCAGTCGGCAGACCAGCATAGCACTCATCGCATGAGGACAGAGACCAAAGCAGCCAAGACCCTGTGCATCATCATGGGTTGCTTCTGCCTCTGCTGGGCACCATTCTTTGTCACCAATATTGTGGATCCTTTCATAGACTACACTGTCCCTGGGCAGGTGTGGACTGCTTTCCTCTGGCTCGGCTATATCAATTCCGGGTTGAACCCTTTTCTCTACGCCTTCTTGAATAAGTCTTTTAGACGTGCCTTCCTCATCATCCTCTGCTGTGATGATGAGCGCTACCGAAGACCTTCCATTCTGGGCCAGACTGTCCCTTGTTCAACCACAACCATTAATGGATCCACACATGTACTAAGGACAGATTTTCTGTTTGACAGAGACATTTTAGCAAGGTATTGGACAAAGCCTGCCAGAGCGGGTCCATTCTCAGGCACATTGAGCATTAGATGTTTGACTGCAAGGAAGCCTGTCTTGGGGGATGCAGTGGAGTGTGGTGGCCAGTGGGAGAGTCAGTGTCACCCGCCAGCAACTTCTCCTTTGGTGGCTGCTCAGCCCAGTGACACTTAGGCCCCTGGGACAATGACCCAGAAGACAGCCATGCCTCCGAAAGAGGGCCAGGTCCTAAGCTGCTGCTTGTGCGCGACTGCACCCGGTATTCTCTTCACCTGAGGCTTTCCGTCCGCCAGTGCAGGAACCCGGTGCTCGCTGGGCTTTTCCTCTGAGATTCCAGCAGGTGGCGCTGGAGGGAGTCAGGGGACATAATGGCCTCCTTGTTCACTTTTTATTTCCCAACACTCCCTCTTCCCAGATTCTCTGCTTTTGCCGCGTGGTCTCTGGTGTCTCTGACATGTTCCCGTCACTCACTGTGTCTGATCTGTCTCACTCACGTTCTAAGCACGATAGCTTGCTGCGTCCTATCTCGGTCACTGATGTCTCCAAATACGCCTTCTTTGCTGTGTGGGCTTCCAGATATCAACAAAATACTCCCTCTCTGTTCCTGTCTTAGCCAGAGGCGTCGCTCCTGTCTTGCTTACTTGCTTGGTCACGTCCGTCACAGCCATGTTCAAGCTATCCCCTGCCTCTTCTTCGCCCTGTTCTGTGCCAGACGCTAACACACTTTTCTCCCTGTGTCTGGGTCCAAACGTGCATCCTCTTGGTTGGCCCCCTTGTCAGTCCCAGACGTCCACTTGCTCTTCCTTATGTCCAATGTCTGTCCTCCTCTTCAGGACATGCTCCATTTATTTTCCATGGAAGACACAGGGTCAGAGATCTGTCAGAGGAAAATGGTCTCCACAGCAACTCTGGAGGGAAGCTGAAGACAGATAGCTTCCTCCTGGTGTCCTGGCCGCTGGCGTGTCTGCCCTGAGATGCTCGCTCTCCTGCTGGGCTAGAACGGGACGTCTTCTAAAAAGCCTTGGGCAGGGCTAGCAACATCGTAGGTGTTTATTAGGCTTCAGGATCTTTCCCATGAACTTGTGATATCATTTTGAAACCCTTCAATCTGGCTTTGTTGCTGCCAAAGGAGATAACTGTTGGAAGTTCACACTGGGGAAACTCATTTTCCCCTGCAGCTCCCCCAAGGTGCAGGAGGGCAGGATCCAGCAGCACTGGGGATGCCGAGGCAATGCTGTACTTGAAGCCAGAGGACCCAAATTGCCGCCCTGGCTGTGCTGCAAACAAGCTGTGAGATCTTGAATAGTGTTCTTTCATCTCTGGACCCCACTTTCCACATCTGTGCACTCAAGCTTGGACTAGAGGACCTTTAAGTTTTCTTTCTGTTTCTAAGACAACAGTATGCTGCTATAGCCGTCTGCTACCTCGAGAAGCCATGGCCTGCCTGCCTTTTGGCTTTGTTTCAAAGGAACGACCCTCCTCATTTGGGCCTTTTAGACCCCTGACCTTTCCAGCTTTGTCCTTCAGATTAATCTGGATGTCTTTTATTCAAAGCTGAGTCAGTGGCTCTTGCGAATGCCCCATAGCAGAAGTCCCGGGAAGGTAAATGCCAAACCCAGAGCCATGTGAGATTCTGACTTTGATCTTGGCTGTGGTTTAATAGCATCTCAGGATTATTTATCATATTCTCTTTTTTTCTTATTGCTTATAAGGTGTCAGTTATTGTTTCCATTGTTGTTTACTTTAGTTCTGGAACTGAAACCGACAGGAAGAAACTTTGGAATAAGGAAGAGAAGATTGACCAAACCATCCAAATGCCAAAAAG hsa-mir-103-3p micro rna 103-3p mirna micro rna micro RNA micro-RNA micro-rna mirna miRNA human Homo sapiens Human AGCAGCAUUGUACAGGGCUAUGA hsa_mir_103 3p-mrna_htr4-2 3534953 N/A 3534954 regulator target regulator target regulator target modulator target neutral component neutral component over-expressed over expressed level over-expressed living cell living cell engineered engineered tag luciferase tag luciferase tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false r.[1399_1401delinscgg, 2567_2569delinscgg] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false r.2567_2569delinscgg mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false r.1395t > c mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false r.[1399_1401delinscgg, 2567_2569 delinscgg,2439_2441delinscgc] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false r.2439_2441delinscgc mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false N/A 3534955 predetermined predetermined participant predetermined regulator regulator modulator regulator neutral component neutral component engineered engineered purified purified physical association physical association false false false Fig 6A'' B'' down-regulates quantity by destabilization