IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ borroto_escuela-2017-4 human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. bret bioluminescence resonance energy transfer BRET LRET LRET BRET bret tag visualisation tag visualisation Borroto-Escuela DO., Li X., Tarakanov AO., Savelli D., Narváez M., Shumilov K., Andrade-Talavera Y., Jimenez-Beristain A., Pomierny B., Díaz-Cabiale Z., Cuppini R., Ambrogini P., Lindskog M., Fuxe K., Borroto-Escuela DO., Li X., Tarakanov AO., Savelli D., Narváez M., Shumilov K., Andrade-Talavera Y., Jimenez-Beristain A., Pomierny B., Díaz-Cabiale Z., Cuppini R., Ambrogini P., Lindskog M., Fuxe K. The authors have confirmed that constructs used in the BRET assay were of Human origin. Species origin of HT2A is Human (see Ref 40) and HT1A is Human (Ref 10). The authors have confirmed that constructs used in the BRET assay were of Human origin. dasiel.borroto.escuela@ki.se ACS omega 2017 Only protein-protein interactions imex curation imex curation borroto_escuela-2017-1 rat-brain-hippocampus Hippocampus of rat brain rat-brain-hippocampus Hippocampus of rat brain pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Borroto-Escuela DO., Li X., Tarakanov AO., Savelli D., Narváez M., Shumilov K., Andrade-Talavera Y., Jimenez-Beristain A., Pomierny B., Díaz-Cabiale Z., Cuppini R., Ambrogini P., Lindskog M., Fuxe K., Borroto-Escuela DO., Li X., Tarakanov AO., Savelli D., Narváez M., Shumilov K., Andrade-Talavera Y., Jimenez-Beristain A., Pomierny B., Díaz-Cabiale Z., Cuppini R., Ambrogini P., Lindskog M., Fuxe K. Species origin of HT2A is Human (see Ref 40) and HT1A is Human (Ref 10). The authors have confirmed that constructs used in the BRET assay were of Human origin. dasiel.borroto.escuela@ki.se ACS omega 2017 Only protein-protein interactions imex curation imex curation borroto_escuela-2017-2 rat-brain-hippocampus Hippocampus of rat brain rat-brain-hippocampus Hippocampus of rat brain confocal microscopy confocal microscopy immunohistochemistry immunohistochemistry Borroto-Escuela DO., Li X., Tarakanov AO., Savelli D., Narváez M., Shumilov K., Andrade-Talavera Y., Jimenez-Beristain A., Pomierny B., Díaz-Cabiale Z., Cuppini R., Ambrogini P., Lindskog M., Fuxe K., Borroto-Escuela DO., Li X., Tarakanov AO., Savelli D., Narváez M., Shumilov K., Andrade-Talavera Y., Jimenez-Beristain A., Pomierny B., Díaz-Cabiale Z., Cuppini R., Ambrogini P., Lindskog M., Fuxe K. Species origin of HT2A is Human (see Ref 40) and HT1A is Human (Ref 10). The authors have confirmed that constructs used in the BRET assay were of Human origin. dasiel.borroto.escuela@ki.se ACS omega 2017 Only protein-protein interactions imex curation imex curation borroto_escuela-2017-3 rat-brain-cortex Rattus norvegicus brain cortex brain_cortex Brain cortex [Cerebral cortex; Cerebrum cortex; Cortex; Pallium] pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Borroto-Escuela DO., Li X., Tarakanov AO., Savelli D., Narváez M., Shumilov K., Andrade-Talavera Y., Jimenez-Beristain A., Pomierny B., Díaz-Cabiale Z., Cuppini R., Ambrogini P., Lindskog M., Fuxe K., Borroto-Escuela DO., Li X., Tarakanov AO., Savelli D., Narváez M., Shumilov K., Andrade-Talavera Y., Jimenez-Beristain A., Pomierny B., Díaz-Cabiale Z., Cuppini R., Ambrogini P., Lindskog M., Fuxe K. anterior cingulate cortex Species origin of HT2A is Human (see Ref 40) and HT1A is Human (Ref 10). The authors have confirmed that constructs used in the BRET assay were of Human origin. dasiel.borroto.escuela@ki.se ACS omega 2017 Only protein-protein interactions imex curation imex curation 5ht2a_human 5-hydroxytryptamine receptor 2A HTR2A HTR2 Serotonin receptor 2A protein protein human Homo sapiens Human MDILCEENTSLSSTTNSLMQLNDDTRLYSNDFNSGEANTSDAFNWTVDSENRTNLSCEGCLSPSCLSLLHLQEKNWSALLTAVVIILTIAGNILVIMAVSLEKKLQNATNYFLMSLAIADMLLGFLVMPVSMLTILYGYRWPLPSKLCAVWIYLDVLFSTASIMHLCAISLDRYVAIQNPIHHSRFNSRTKAFLKIIAVWTISVGISMPIPVFGLQDDSKVFKEGSCLLADDNFVLIGSFVSFFIPLTIMVITYFLTIKSLQKEATLCVSDLGTRAKLASFSFLPQSSLSSEKLFQRSIHREPGSYTGRRTMQSISNEQKACKVLGIVFFLFVVMWCPFFITNIMAVICKESCNEDVIGALLNVFVWIGYLSSAVNPLVYTLFNKTYRSAFSRYIQCQYKENKKPLQLILVNTIPALAYKSSQLQMGQKKNSKQDAKTTDNDCSMVALGKQHSEEASKDNSDGVNEKVSCV 5ht1a_human 5-hydroxytryptamine receptor 1A HTR1A ADRB2RL1 ADRBRL1 G-21 Serotonin receptor 1A protein protein human Homo sapiens Human MDVLSPGQGNNTTSPPAPFETGGNTTGISDVTVSYQVITSLLLGTLIFCAVLGNACVVAAIALERSLQNVANYLIGSLAVTDLMVSVLVLPMAALYQVLNKWTLGQVTCDLFIALDVLCCTSSILHLCAIALDRYWAITDPIDYVNKRTPRRAAALISLTWLIGFLISIPPMLGWRTPEDRSDPDACTISKDHGYTIYSTFGAFYIPLLLMLVLYGRIFRAARFRIRKTVKKVEKTGADTRHGASPAPQPKKSVNGESGSRNWRLGVESKAGGALCANGAVRQGDDGAALEVIEVHRVGNSKEHLPLPSEAGPTPCAPASFERKNERNAEAKRKMALARERKTVKTLGIIMGTFILCWLPFFIVALVLPFCESSCHMPTLLGAIINWLGYSNSLLNPVIYAYFNKDFQNAFKKIIKCKFCRQ 5ht2a_rat 5-hydroxytryptamine receptor 2A Htr2a Htr2 Serotonin receptor 2A protein protein rat Rattus norvegicus (Rat) Rat MEILCEDNISLSSIPNSLMQLGDGPRLYHNDFNSRDANTSEASNWTIDAENRTNLSCEGYLPPTCLSILHLQEKNWSALLTTVVIILTIAGNILVIMAVSLEKKLQNATNYFLMSLAIADMLLGFLVMPVSMLTILYGYRWPLPSKLCAIWIYLDVLFSTASIMHLCAISLDRYVAIQNPIHHSRFNSRTKAFLKIIAVWTISVGISMPIPVFGLQDDSKVFKEGSCLLADDNFVLIGSFVAFFIPLTIMVITYFLTIKSLQKEATLCVSDLSTRAKLASFSFLPQSSLSSEKLFQRSIHREPGSYAGRRTMQSISNEQKACKVLGIVFFLFVVMWCPFFITNIMAVICKESCNENVIGALLNVFVWIGYLSSAVNPLVYTLFNKTYRSAFSRYIQCQYKENRKPLQLILVNTIPALAYKSSQLQVGQKKNSQEDAEQTVDDCSMVTLGKQQSEENCTDNIETVNEKVSCV This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. This receptor is involved in tracheal smooth muscle contraction, bronchoconstriction, and control of aldosterone production mint 5ht1a_rat 5-hydroxytryptamine receptor 1A Htr1a 5ht1a Serotonin receptor 1A protein protein rat Rattus norvegicus (Rat) Rat MDVFSFGQGNNTTASQEPFGTGGNVTSISDVTFSYQVITSLLLGTLIFCAVLGNACVVAAIALERSLQNVANYLIGSLAVTDLMVSVLVLPMAALYQVLNKWTLGQVTCDLFIALDVLCCTSSILHLCAIALDRYWAITDPIDYVNKRTPRRAAALISLTWLIGFLISIPPMLGWRTPEDRSDPDACTISKDHGYTIYSTFGAFYIPLLLMLVLYGRIFRAARFRIRKTVRKVEKKGAGTSLGTSSAPPPKKSLNGQPGSGDWRRCAENRAVGTPCTNGAVRQGDDEATLEVIEVHRVGNSKEHLPLPSESGSNSYAPACLERKNERNAEAKRKMALARERKTVKTLGIIMGTFILCWLPFFIVALVLPFCESSCHMPALLGAIINWLGYSNSLLNPVIYAYFNKDFQNAFKKIIKCKFCRR htr1a-htr2a-4 3559597 N/A 3559601 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed C-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 3559602 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed region renilla luciferase renilla luciferase protein tag renilla luciferase undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false proximity proximity close-contact colocalization neighbourhood interaction false false false Figure 4 htr1a-htr2a-5 3559597 N/A 3559602 competitor competitor competitor unspecified role unspecified role over-expressed over expressed level over-expressed N/A 3559601 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed C-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 3559602 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed region renilla luciferase renilla luciferase protein tag renilla luciferase undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false proximity proximity close-contact colocalization neighbourhood interaction false false false Figure 4B htr1a-htr1a-1 3559597 N/A 3559601 competitor competitor competitor unspecified role unspecified role over-expressed over expressed level over-expressed N/A 3559601 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed C-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 3559602 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed region renilla luciferase renilla luciferase protein tag renilla luciferase undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false proximity proximity close-contact colocalization neighbourhood interaction false false false Figure 4B htr1a-htr2a-1 3559598 N/A 3559603 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3559604 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level proximity proximity close-contact colocalization neighbourhood interaction false false false Figure 1 PLA-positive clusters (5-HT1A–5-HT2A isoreceptor complexes) were found in the pyramidal cell layer of the CA1, CA2, and CA3 regions. htr1a-htr2a-2 3559599 N/A 3559603 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3559604 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level colocalization colocalization false false false Figure 2 CA1 pyramidal cell layer htr1a-htr2a-3 3559600 N/A 3559603 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3559604 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level proximity proximity close-contact colocalization neighbourhood interaction false false false Figure 3 A high density of PLA-positive clusters (5-HT1A–5-HT2A isoreceptor complexes) were found in layer III, and a low to moderate number of PLA clusters in layer II.