IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ fukuda-2016-2 human-sh_sy5y Homo sapiens SH-SY5Y cell line sh_sy5y Human neuroblastoma cells anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Fukuda T., Nagashima S., Abe T., Kiyonari H., Inatome R., Yanagi S. Results obtained using alpha-tubulin antibodies have not been included as they cannot be assigned to a single gene product. syanagi@toyaku.ac.jp,syanagi@toyaku.ac.jp EMBO reports 2016 Only protein-protein interactions imex curation imex curation fukuda-2016-1 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Fukuda T., Nagashima S., Abe T., Kiyonari H., Inatome R., Yanagi S. Results obtained using alpha-tubulin antibodies have not been included as they cannot be assigned to a single gene product. syanagi@toyaku.ac.jp,syanagi@toyaku.ac.jp mouse brain cDNA EMBO reports 2016 Only protein-protein interactions imex curation imex curation fukuda-2016-3 mouse-brain Mus musculus brain brain Brain [cerebrum] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Fukuda T., Nagashima S., Abe T., Kiyonari H., Inatome R., Yanagi S. Results obtained using alpha-tubulin antibodies have not been included as they cannot be assigned to a single gene product. syanagi@toyaku.ac.jp,syanagi@toyaku.ac.jp EMBO reports 2016 Only protein-protein interactions imex curation imex curation d3zey0_rat Ccdc141 LOC311134 protein protein rat Rattus norvegicus (Rat) Rat MSCKESPHVGVSTTTVSSVAVQAGDSKIVIAVVKCGKWVRLQLAEAQPNLLEIGSSQDETRKLLHDHELLLAKLKALEDRVWGLLQEADRTAEANKEQSEVYDAMAQTLGEAWATLVSMLERRRELLGLTSEFFQSALEFAIKIDQAEDFLQNPHEFESAEALQSLLLLHDRHAKELLERSLVLLNKSQQLTDFIEKFKCDGSPVNSELIQGAQSSCLKIDSLLELLQDRRRQLDKHLQQQRQELSQVLQLCLWDQQESQVSCWFQKTIRDLQEQSLGSSLSDNKELIRKHEDLTIKAKEWDLAMEKLKSQALGILLSKDLTEKEHLQLSNQKLNRLQEEFGRLMVDRKAWLTMAHDFFTSANEAFDVLGKVEDYLKLLKSEGLSLPALAAKHEELHREIKDSTAAALQKGRTLISQVDSCRAQVTGIHETMGRIQKRVDRLSQQCTAHQEFALKKQQLATSVDDYLRKVEMSVQEMRLVLATTLDVGSSPSESEKILNKYLELDIQAKETAHALEAAAKITTEKNELELNEVALLPFKVKRLEEELSTLSRSVSYRSQVLQTYIAFRKSSEEVEEQLQSLKEFYLTESPWEDEDDAVVTCQSNSAERKWQLFLKKSFLTQDLSLEFLNLISMAKENEILNVKKEMHIVENVMEKQTNGREELSRLRMAWHLKAMEGKPVKERWETFKEKLKKTTHNVKLLHEVLMPVSALDLGGNLQSMSDLRRRWIAMQPQLQQLHEDVQQIMKEWEVLSGQGVPLKEKAEQLKDLIHLHQRQRERIQEYEEILYKTVQFHQVKEELTHLIKPRELELLAQPMELEGSKEVLMQLGRSQGRRAHVDHLHRLALSLGVDIISSVQRPNCSNISAKSLQQQLEALELDSRDWSAKAKEYEQVLACSLEYCTTREEINELKESFKDIKKKFNNLKFNYSKKNEKSRNLKTLQYQIQQVETYADKIQALRKKMEKVNKKISDSVLSYPSNKANTLVEAMEDLKKHVDDFDKVVTDYKMNLDLTEHLQEVIEECNFWYEDASATVVRVGKYSMECQTREAVDILHRQFQKFVTPSVPQQEERIQEVVSLAQRLYGLEEGQKYAEKIVTRHKEILESITELCGSLVELKEKLMQGDVPKVNSNLEDFHGNSIDLLKEPGRDEQTTFSEERSEGQAQGADVSAINGTREDGLPVGLRQSFDKEDSAQGLTLPEDTLSGEDSECISSDDISLPPLPVTPESPLAPSDMEVEEPASSSALALHISGYRMRTGTGGLGKAPESVLPPPTAFTDGYHNKKDTFTSHFERPYPQFKAEPLLTSRGSGEMSTKLHVNVKCPARMPREVHDKALPPSSQAQEISLGTQEKVHADSNVTKTQDRLHAALDVSPGLSSQSDTSRSHQRQVGPQGDRKNSSAEKSVVSLAGQAPHFSRLLSNVTVTEGSPVTLEVEVTGFPEPTLTWFKKGQKLCADGHLQVLHKDTKHSVFIPKVCEADAGLYVAQAQNSSGTLSSKAILHVTGNHGPPITRINWIVLCIIYVSVSVIYWLLTQ hdac6_mouse Histone deacetylase 6 Histone deacetylase mHDA2 Hdac6 Tubulin-lysine deacetylase HDAC6 protein protein mouse Mus musculus Mouse MTSTGQDSSTRQRKSRHNPQSPLQESSATLKRGGKKCAVPHSSPNLAEVKKKGKMKKLSQPAEEDLVVGLQGLDLNPETRVPVGTGLVFDEQLNDFHCLWDDSFPESPERLHAIREQLILEGLLGRCVSFQARFAEKEELMLVHSLEYIDLMETTQYMNEGELRVLAETYDSVYLHPNSYSCACLATGSVLRLVDALMGAEIRNGMAVIRPPGHHAQHNLMDGYCMFNHLAVAARYAQKKHRIQRVLIVDWDVHHGQGTQFIFDQDPSVLYFSIHRYEHGRFWPHLKASNWSTIGFGQGQGYTINVPWNQTGMRDADYIAAFLHILLPVASEFQPQLVLVAAGFDALHGDPKGEMAATPAGFAHLTHLLMGLAGGKLILSLEGGYNLRALAKGVSASLHTLLGDPCPMLESCVVPCASAQTSIYCTLEALEPFWEVLERSVETQEEDEVEEAVLEEEEEEGGWEATALPMDTWPLLQNRTGLVYDEKMMSHCNLWDNHHPETPQRILRIMCHLEEVGLAARCLILPARPALDSELLTCHSAEYVEHLRTTEKMKTRDLHREGANFDSIYICPSTFACAKLATGAACRLVEAVLSGEVLNGIAVVRPPGHHAEPNAACGFCFFNSVAVAARHAQIIAGRALRILIVDWDVHHGNGTQHIFEDDPSVLYVSLHRYDRGTFFPMGDEGASSQVGRDAGIGFTVNVPWNGPRMGDADYLAAWHRLVLPIAYEFNPELVLISAGFDAAQGDPLGGCQVTPEGYAHLTHLLMGLAGGRIILILEGGYNLASISESMAACTHSLLGDPPPQLTLLRPPQSGALVSISEVIQVHRKYWRSLRLMKMEDKEECSSSRLVIKKLPPTASPVSAKEMTTPKGKVPEESVRKTIAALPGKESTLGQAKSKMAKAVLAQGQSSEQAAKGTTLDLATSKETVGGATTDLWASAAAPENFPNQTTSVEALGETEPTPPASHTNKQTTGASPLQGVTAQQSLQLGVLSTLELSREAEEAHDSEEGLLGEAAGGQDMNSLMLTQGFGDFNTQDVFYAVTPLSWCPHLMAVCPIPAAGLDVSQPCKTCGTVQENWVCLTCYQVYCSRYVNAHMVCHHEASEHPLVLSCVDLSTWCYVCQAYVHHEDLQDVKNAAHQNKFGEDMPHSH e9q8q6_mouse Ccdc141 protein protein mouse Mus musculus Mouse MSCKESPHVGASTTTVSSVAVHAGDSKIVIAVVKCGKWVRLQLAESQPNLLEIGSSQDETKKLLHDHELLLAKLKALEDRVWELLREADRTAEANKAQSQVYDAMAQTLGEAWATLVSMLERRRELLGLTSEFFQSALEFAIKIDQAEAFLQNPHEFESTEALQSLLLLHDRHAKELLERSLDLLNKSQQLTDFIEKFKCEGSTMNSELIQGAQSSCLKIDSLLELLQDRRRQLDKYLQQQRQELSQVLQLCLWDQQENQVSSWFQKAIRDLQEQSLGASLSDNRELICKHEDLIVKAKEWDSAVEKLKSQALGILLSKDLAGKEHLQLSNQKLNRLQEEFGRLMVERKAWLSMANDFFTSANKSFDVLGKVEAYLKLLKSEGLSLPVLAAKHEELHREIKDSTATALQKGRTLISQVDSCRSRVTGIHEMMGYIQNRVDCLTEQCTAHEEFARKRQQLATSVDDYLRKVEMSIQEIRPILATTLDVASSPSESEKILNKYLELDIQVKETAHALEAAAKIMTEKNELELNEVALLPLKVKWLEEELSTLGRSISCRSRILQTYVAFRKSSEEAEEQLQSLKEFYLTEIPWKDEDDAVVKCQSNSAERKWQLFLKKSFLTQDLSLEFLNLINMAKENEILNVKNEMHIMENIMEKQTNGREELSHLRVAWYLKAIEGKPAREQWEMFKEKLTKTTHSVKLLHEVLMPVSALDLGGSLQSTSDLRRRWIAMKPQLQQLHEDVQQITKEWEVLSSQGAPLKEKAEQLKDLVHLHRRQRERIQEYEEILYKTVQFHQVKEELVHLIKPRELELLAQPMELASSEEVQMQLGRSQERRAHVDHLHQLALTLGVDIISSVQQPNCSNISAKNLQQQLEALELESRSWSAQAKEHERVLSCSLEYCTARDEISELKESFKDIKKKFNNLKFNYSKKNEKSRNLKTLQYQIQQVDTYAEKIQALRKKMEKVNNKTSDSFLSYPSNKANMLSEAMEDLKKNVDDFDKVVTDYKMNLDLTEHLQEVIEECNFWYEDASATVVRVGKYSMECQTREAVDILHRQFNKFITPSVPQQEERIQEVIDLAQRLYGLEEGQKYAEKIVTRHKEILESITELCGSLVELKEKLMQGEVPKMNSDLEDFHDNCIDLLKGPGKDDQKTFSEERNEGQVQGADVLAVNGTREDGLPMDLRRTSSDKEDSAQGLILPEDTLSGEESECISSDDISLPPLPVSPESPLAPSDMEVEELTSSSALALHISGYRMHPGTGGLGKAQESALPSPIAFADGGHSKKDTFTSHFERPYPQLKAESSLASRGSAEMSTKLHINVKCPASMPHEVHDKALQQCSQARESTLEMQEKVHADSNVTKTQDRLHAALDVSPGLGSQPDTSESHQRRVGPQGNKKNSSAENSVVSLAGQAPHFSRLLSNVTVMEGSPVTLEVEVTGFPEPTLTWFKKGQKLCADGHLQVLHKDTKHSVFIPKVCEADAGLYVAQAQNSSGTLSSKAILHVTGNHGPPITRLNWIMLCIIYVSVSVIYWLLTR ccdc141-hdac6-2 3575931 N/A 3575934 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3575935 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false second HDAC domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Figure 3A/D ccdc141-hdac6-3 3575931 N/A 3575934 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 3575935 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Figure 3A/C ccdc141-hdac6-1 3575932 N/A 3575934 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed N‐terminal spectrin domain sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3575935 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false no figure hdac6-ccdc141-1 3575933 N/A 3575936 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3575935 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false Figure 3B