IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ haapasalo-2015-4 in vitro In vitro solid phase assay solid phase assay tag visualisation tag visualisation Haapasalo K., van Kessel K., Nissilä E., Metso J., Johansson T., Miettinen S., Varjosalo M., Kirveskari J., Kuusela P., Chroni A., Jauhiainen M., van Strijp J., Jokiranta TS. K.J.Haapasalo-Tuomainen@umcutrecht.nl The Journal of biological chemistry 2015 Only protein-protein interactions imex curation imex curation haapasalo-2015-1 in vitro In vitro pull down pull down ms participant Identification by mass spectrometry ms participant Haapasalo K., van Kessel K., Nissilä E., Metso J., Johansson T., Miettinen S., Varjosalo M., Kirveskari J., Kuusela P., Chroni A., Jauhiainen M., van Strijp J., Jokiranta TS. amino acid boundaries of FH SCR domains taken from Ref. 16 K.J.Haapasalo-Tuomainen@umcutrecht.nl The Journal of biological chemistry 2015 Only protein-protein interactions imex curation imex curation haapasalo-2015-2 in vitro In vitro mst microscale thermophoresis mst predetermined predetermined participant predetermined Haapasalo K., van Kessel K., Nissilä E., Metso J., Johansson T., Miettinen S., Varjosalo M., Kirveskari J., Kuusela P., Chroni A., Jauhiainen M., van Strijp J., Jokiranta TS. K.J.Haapasalo-Tuomainen@umcutrecht.nl The Journal of biological chemistry 2015 Only protein-protein interactions imex curation imex curation haapasalo-2015-5 in vitro In vitro elisa enzyme linked immunosorbent assay ELISA ELISA elisa antibody detection identification by antibody antibody detection Haapasalo K., van Kessel K., Nissilä E., Metso J., Johansson T., Miettinen S., Varjosalo M., Kirveskari J., Kuusela P., Chroni A., Jauhiainen M., van Strijp J., Jokiranta TS. K.J.Haapasalo-Tuomainen@umcutrecht.nl The Journal of biological chemistry 2015 Only protein-protein interactions imex curation imex curation apoe_human Apolipoprotein E APOE protein protein human Homo sapiens Human MKVLWAALLVTFLAGCQAKVEQAVETEPEPELRQQTEWQSGQRWELALGRFWDYLRWVQTLSEQVQEELLSSQVTQELRALMDETMKELKAYKSELEEQLTPVAEETRARLSKELQAAQARLGADMEDVCGRLVQYRGEVQAMLGQSTEELRVRLASHLRKLRKRLLRDADDLQKRLAVYQAGAREGAERGLSAIRERLGPLVEQGRVRAATVGSLAGQPLQERAQAWGERLRARMEEMGSRTRDRLDEVKEQVAEVRAKLEEQAQQIRLQAEAFQARLKSWFEPLVEDMQRQWAGLVEKVQAAVGTSAAPVPSDNH cfah_human Complement factor H CFH HF HF1 HF2 H factor 1 protein protein human Homo sapiens Human MRLLAKIICLMLWAICVAEDCNELPPRRNTEILTGSWSDQTYPEGTQAIYKCRPGYRSLGNVIMVCRKGEWVALNPLRKCQKRPCGHPGDTPFGTFTLTGGNVFEYGVKAVYTCNEGYQLLGEINYRECDTDGWTNDIPICEVVKCLPVTAPENGKIVSSAMEPDREYHFGQAVRFVCNSGYKIEGDEEMHCSDDGFWSKEKPKCVEISCKSPDVINGSPISQKIIYKENERFQYKCNMGYEYSERGDAVCTESGWRPLPSCEEKSCDNPYIPNGDYSPLRIKHRTGDEITYQCRNGFYPATRGNTAKCTSTGWIPAPRCTLKPCDYPDIKHGGLYHENMRRPYFPVAVGKYYSYYCDEHFETPSGSYWDHIHCTQDGWSPAVPCLRKCYFPYLENGYNQNYGRKFVQGKSIDVACHPGYALPKAQTTVTCMENGWSPTPRCIRVKTCSKSSIDIENGFISESQYTYALKEKAKYQCKLGYVTADGETSGSITCGKDGWSAQPTCIKSCDIPVFMNARTKNDFTWFKLNDTLDYECHDGYESNTGSTTGSIVCGYNGWSDLPICYERECELPKIDVHLVPDRKKDQYKVGEVLKFSCKPGFTIVGPNSVQCYHFGLSPDLPICKEQVQSCGPPPELLNGNVKEKTKEEYGHSEVVEYYCNPRFLMKGPNKIQCVDGEWTTLPVCIVEESTCGDIPELEHGWAQLSSPPYYYGDSVEFNCSESFTMIGHRSITCIHGVWTQLPQCVAIDKLKKCKSSNLIILEEHLKNKKEFDHNSNIRYRCRGKEGWIHTVCINGRWDPEVNCSMAQIQLCPPPPQIPNSHNMTTTLNYRDGEKVSVLCQENYLIQEGEEITCKDGRWQSIPLCVEKIPCSQPPQIEHGTINSSRSSQESYAHGTKLSYTCEGGFRISEENETTCYMGKWSSPPQCEGLPCKSPPEISHGVVAHMSDSYQYGEEVTYKCFEGFGIDGPAIAKCLGEKWSHPPSCIKTDCLSLPSFENAIPMGEKKDVYKAGEQVTYTCATYYKMDGASNVTCINSRWTGRPTCRDTSCVNPPTVQNAYIVSRQMSKYPSGERVRYQCRSPYEMFGDEEVMCLNGNWTEPPQCKDSTGKCGPPPPIDNGDITSFPLSVYAPASSVEYQCQNLYQLEGNKRITCRNGQWSEPPKCLHPCVISREIMENYNIALRWTAKQKLYSRTGESVEFVCKRGYRLSSRSHTLRTTCWDGKLEYPTCAKR apoe-cfh-3 3633712 N/A 3633716 western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey purified purified region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 3633717 predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified human-serum Homo sapiens serum Serum direct interaction direct interaction false false false 7A/C The APOE*3 variant . apoe-cfh-5 3633712 N/A 3633717 unspecified role unspecified role bait bait purified purified human-serum Homo sapiens serum Serum N/A 3633716 western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey purified purified region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 3633717 predetermined predetermined participant predetermined competitor competitor competitor unspecified role unspecified role purified purified region fitc labelled fluorescein isothiocyanate label fluorescein isothiocyanate labbeled FITC labelled FITC labelled fluorescein isothiocyanate labbeled fitc labelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false SCR/Sushi_domains 5-7 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false picpa Pichia pastoris (Yeast) Yeast Pichia pastoris physical association physical association false false false 7C-2 The APOE*3 variant . cfh-apoe-3 3633712 N/A 3633716 competitor competitor competitor unspecified role unspecified role purified purified ecoli Escherichia coli (strain K12) N/A 3633716 western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey purified purified region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 3633717 predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified human-serum Homo sapiens serum Serum physical association physical association false false false 7A-2 The APOE*3 variant . cfh-apoe-4 3633712 N/A 3633716 western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey purified purified region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 3633717 predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified region fitc labelled fluorescein isothiocyanate label fluorescein isothiocyanate labbeled FITC labelled FITC labelled fluorescein isothiocyanate labbeled fitc labelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false SCR/Sushi_domains 5-7 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false picpa Pichia pastoris (Yeast) Yeast Pichia pastoris direct interaction direct interaction false false false 7B-1 The APOE*3 variant . cfh-apoe-5 3633712 N/A 3633716 western blot western blot Immuno blot Immuno blot competitor competitor competitor unspecified role unspecified role purified purified ecoli Escherichia coli (strain K12) N/A 3633717 predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified region fitc labelled fluorescein isothiocyanate label fluorescein isothiocyanate labbeled FITC labelled FITC labelled fluorescein isothiocyanate labbeled fitc labelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false SCR/Sushi_domains 5-7 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false picpa Pichia pastoris (Yeast) Yeast Pichia pastoris N/A 3633716 western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey purified purified region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false 7B-2 The APOE*3 variant . cfh-apoe-1 3633713 N/A 3633716 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level human-serum Homo sapiens serum Serum N/A 3633717 unspecified role unspecified role bait bait purified purified SCR/Sushi_domains 5-7 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false picpa Pichia pastoris (Yeast) Yeast Pichia pastoris physical association physical association false false false 5A, Table 1 This is probably the APOE*3 variant. cfh-apoe-2 3633714 N/A 3633716 unspecified role unspecified role neutral component neutral component purified purified red_dye dye labelled dye label dye labelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 3633717 unspecified role unspecified role neutral component neutral component purified purified SCR/Sushi_domains 5-7 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false picpa Pichia pastoris (Yeast) Yeast Pichia pastoris direct interaction direct interaction false false false 3633714 5B The APOE*3 variant . cfh-apoe-6 3633715 N/A 3633716 western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait purified purified P02649:p.Cys130Arg mutation mutation certain certain sequence position certain certain certain sequence position certain false The APOE*4 variant :P02649 VAR_000652. region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3633717 predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified region fitc labelled fluorescein isothiocyanate label fluorescein isothiocyanate labbeled FITC labelled FITC labelled fluorescein isothiocyanate labbeled fitc labelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false SCR/Sushi_domains 5-7 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false picpa Pichia pastoris (Yeast) Yeast Pichia pastoris direct interaction direct interaction false false false 7D The APOE*4 variant :P02649 VAR_000652.