IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ sharma-2014-1 mouse-cardiac myocyt Mus musculus cardiac_myocyte cardiac myocyte confocal microscopy confocal microscopy immunostaining immunostaining Sharma P., Abbasi C., Lazic S., Teng AC., Wang D., Dubois N., Ignatchenko V., Wong V., Liu J., Araki T., Tiburcy M., Ackerley C., Zimmermann WH., Hamilton R., Sun Y., Liu PP., Keller G., Stagljar I., Scott IC., Kislinger T., Gramolini AO. thomas.kislinger@utoronto.ca,anthony.gramolini@utoronto.ca Nature communications 2014 Only protein-protein interactions imex curation imex curation sharma-2014-2 mouse-cardiac myocyt Mus musculus cardiac_myocyte cardiac myocyte anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Sharma P., Abbasi C., Lazic S., Teng AC., Wang D., Dubois N., Ignatchenko V., Wong V., Liu J., Araki T., Tiburcy M., Ackerley C., Zimmermann WH., Hamilton R., Sun Y., Liu PP., Keller G., Stagljar I., Scott IC., Kislinger T., Gramolini AO. thomas.kislinger@utoronto.ca,anthony.gramolini@utoronto.ca Nature communications 2014 Only protein-protein interactions imex curation imex curation tmm65_mouse Transmembrane protein 65 Tmem65 protein protein mouse Mus musculus Mouse MSRLLPLLGSRTARSLRPGPAAAPRLPSWCCCGRGLLALGVPGGPRLLGTHPKKEPMEALNTAQGARDFIYSLHSTERSCLLKELHRFESIAIAQEKLEALPPTPGQLRYVFFHNAIPFVGFGFLDNAIMIVAGTQIELSIGIILGISTMAAAALGNLVSDLAGLGLAGYVEALASRLGLSIPDLTPKQVDMWQTRVSTHLGKAVGVTIGCILGMFPLIFFGGSEEDEKLETTN desp_mouse Desmoplakin Dsp protein protein mouse Mus musculus Mouse MSCNGGSHPRINTLGRMTRAESGPDLRYEMTYSGGGGGGGGGGGGGTSRTFYSHSRRCTVNDQNSDGYCQTGTMSRHQNQNTIQEMLQNCSDCLMRAELIAQPELKFGEGMQLAWNRELDEYFTQANDQMEIIDGLIREMRQMGQPCDAYQKRLLQLQEQMRALYKAISVPRVRRASSKGAGGYTCQSGSGWDEFTKRLTGECLGWMRQQREEMDLMAWGVDAGSVEQHINSHRSIHNTIGDYRWQLDKIKADLREKSAIYQLEEEYENLLKASFERMDHLRQLQNIIQATSREIMWINDCEEEELLYDWSDKNTNIAQKQEAFSIRMSQLEVKEKELNKLKQESDQLVLNQHPASDKIEAYMDTLQTQWSWILQITKCIDVHLKENAAYFQFFEEAQSTEAYLKGLQDSIRKKYPCDKNMPLQHLLEQIKELEKEREKIIEYKRQVQNLVNKSKKIVQLKPRNPDYRSNKPIILRALCDYKQDQKIVHKGDECILKDNNERSKWYVTGPGGVDMLVPSVGLIIPPPNPLAVDLSCKIEQYYEAILALWNQLYINMKSLVSWHYCMIDIEKIRAMTIAKLKTMRQEDYMKTIEDLELHYQDFIKNSQGSEMFGDDDKRRMQSQFTDAQKHYQTLVIQLPGHPQHQTVTKTEITHLGTCQDVNHNKVIETNRENDKQETWLLMELQKIRRQMEHCEARMTLKNLLLAEQGSTHHITVKINELKSVQNDSQALAEVLNQLKDMLANFRGSEKYCYLQNEIFGLFQKLENINGVSDGYLNSLCSVRALLQAILQTEDMLKVYEARLTEEETVCLDLDKVEAYRCGLKKIKNDLNLKKSLLATMKTELQKAQQIHSQSSQQYPLYDLDLGKFTEKVTQLTDRWQKIDKQIDFRLWDLEKQIKQLRNYRDNYQSFCKWLYDAKRRQDSLESMKFGDSNTVMRFLNEQKNLHSEISGKRDKSEEVHKIAELCANSIKDYELQLASYTSGLETLLNIPIKRTMVQSPSGVILQEAADIHARYIELLTRSGDYYRFLSEMLKSLEDLKLKNTKIEVLEEELRLARDANSENCNKNKFLDQNLQKYQAECSQFKAKLVSLEELKRQAELDGKSAKQNLDKCYGQIKELNEKITRLTYEIEDEKRRRKTVEDRFDQQKNDYDQLQKARQCEKENLSWQKLESEKAIKEKEYEIERLRVLLQEEGARKREYENELAKVRNHYNEEMSNLRNKYETEINITKTTIKEISMQKEDDSKNLRNQMDRLSRENRDLKDEIVRLNDSILQATEQRRRAEENALQQKACGSETMQKKQRLEIELKQVIQQRSEDNARHKQSLEEAAKTIQDKNKEIERLKAEYQEEAKRRWEYENELSKVRNSYDEEIISLKNQFETEINITKTTIHQLTMQKEEDTSGYRAQIDNLTRENRSLCEEVKRLKNTLAQTTENLRRVEENAQQQKATGSEMSQRKQQLEIELRQVTQMRTEESMRYKQSLDDAAKTIQDKNKEIERLKQLVDKETNERKCLEDENSKLQRVQYDLQKANNSATEAMSKLKVQEQELTRLRIDYERVSQERTVKDQDITRIQSSLKDLQLQKQKAEEELSRLKRTASDESSKRKMLEEELEAMRRSLKEQAVKITNLTQQLEQASIVKKRSEDDLRQQRDVLDGHVREKQRTQEELRRLSLDVEALRRQLVQEQENVKQAHLRNEHFQKAIEDKSRSLNESKIEIERLQSLTENLTKEHLMLEEELRNLRLEYDDLRRGRSEADSDKNSTISELRSQLQISNNRTLELQGLINDLQRERENLRQEIEKFQKQALEASNRIQESKSQCTQVVQERESLLVKIKVLEQDKARLQRLEDELNRAKATLEAESRVKQRLECEKQQIQNDLNQWKTQYSRKEETIRKIESEREKSEREKNSLRSEIERLQAEIKRIEERCRRKLEDSSRETQSQLESERCRLQKEIEKLRQRPYGSHRETQTEYEWTVDSSKLVFDGLRKKVTAMQLYECQLIDKTTLDKLLKGKKSVEEVASEIQPFLRGAGAIAGASASPKEKYSLVEAKRKKFITPESTVMLLEAQAATGGIIDPHRNEKLTVDNAVARDLIDFDDRQQIYTAEKAITGFDDPFSGKTVSVSEAIKKNLIDRETGMRLLEAQLASGGVVDPVNSVFLPKDVALARGLIDRDLYRSLNDPRDSQKNFVDPITKKKVSYMQLRERCRIEPHTGLLLLSVQKRSMSFQGIRQPVTVTELVDSGILRPSTVNELESGQISYDEVGERIKDFLQGSSCIAGIYNETTKQKLGIYEAMKIGLVRPGTALELLEAQAATGFIVDPVSNLRLPVEEAYKRGLVGIEFKEKLLSAERAVTGYNDPETGNIISLFQAMNKELIEKGHGIRLLEAQIATGGIIDPKESHRLPVDMAYKRGYFNEELSEILSDPSDDTKGFFDPNTEENLTYLQLKERCIKDEETGLCLLPLKEKKKQVQTSQKNTLRKRRVVIVDPETNKEMSVQEAYKKGLIDYDTFKELCEQECEWEEITITGSDGSTRVVLVDRKTGSQYDIQDAIDKGLVDRKFFDQYRSGSLSLTQFADMISLKNGVGNSSGLGGSVNDDVFSSSRHDSVSKISTISSVRNLTIRSSSLSDPLEESSPIAAIFDTENLEKISIAEGIERGIVDSITGQRLLEAQACTGGIIHPTTGQKLSLQDAVNQGLIDQDMATRLKPAQKAFIGFEGVKGKKKMSAAEAVKEKWLPYEAGQRFLEFQFLTGGLVDPEVHGRISTEEAIRKGFIDGRAAQRLQDISSYAKILTCPKTKLKISYKDAMNRSMVEDITGLRLLEAASVSSKGLPSPYNMSAPGSRSGSRSGSRSGSRSGSRSGSRRGSFDATGNSSYSYSYSFSSSSIGGY AfCS Y2H protein. Translated from nucleotide sequence. Similar to DESP_HUMAN Desmoplakin (DP) (250/210 kDa paraneoplastic pemphigus antigen) (95%) cxa1_mouse Gap junction alpha-1 protein Gja1 Cxn-43 Connexin-43 Gap junction 43 kDa heart protein protein protein mouse Mus musculus Mouse MGDWSALGKLLDKVQAYSTAGGKVWLSVLFIFRILLLGTAVESAWGDEQSAFRCNTQQPGCENVCYDKSFPISHVRFWVLQIIFVSVPTLLYLAHVFYVMRKEEKLNKKEEELKVAQTDGVNVEMHLKQIEIKKFKYGIEEHGKVKMRGGLLRTYIISILFKSVFEVAFLLIQWYIYGFSLSAVYTCKRDPCPHQVDCFLSRPTEKTIFIIFMLVVSLVSLALNIIELFYVFFKGVKDRVKGRSDPYHATTGPLSPSKDCGSPKYAYFNGCSSPTAPLSPMSPPGYKLVTGDRNNSSCRNYNKQASEQNWANYSAEQNRMGQAGSTISNSHAQPFDFPDDSQNAKKVAAGHELQPLAIVDQRPSSRASSRASSRPRPDDLEI cdh2-dsp-1 3547035 N/A 3547037 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell N/A 3547038 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell colocalization colocalization false false false Figure 6a gja1-tmem65-2 3547035 N/A 3547037 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell N/A 3547039 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell colocalization colocalization false false false Figure 6a gja1-tmem65-1 3547036 N/A 3547037 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3547039 unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false Figure 6b The Tmem65 monomer and dimer co-immunoprecipitated with Cx43, although significant enrichment was observed in the dimer, compared with levels seen in the input cardiac lysate. dsp-tmem65-1 3547036 N/A 3547037 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3547038 unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false Figure 6b