UniProt UniProt http://www.uniprot.org yu-2014-8 human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference fluorescence imaging fluorescence microscopy fluorescence imaging anti-tag immunocytochemistry anti-tag immunocytochemistry Yu J., Chia J., Canning CA., Jones CM., Bard FA., Virshup DM., Yu J., Chia J., Canning CA., Jones CM., Bard FA., Virshup DM. STF cells - an HEK293 cell line that contains an integrated SuperTOPflash reporter Origin of WNT3A inferred from PMID:22784633. Origin of HA-PORCN inferred from PMID:26257057. Source of ERGIC2 could not be traced so interactions invovling this protein were not added. david.virshup@duke-nus.edu.sg Developmental cell 2014 Only protein-protein interactions imex curation imex curation yu-2014-5 human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Yu J., Chia J., Canning CA., Jones CM., Bard FA., Virshup DM., Yu J., Chia J., Canning CA., Jones CM., Bard FA., Virshup DM. Origin of WNT3A inferred from PMID:22784633. Origin of HA-PORCN inferred from PMID:26257057. Source of ERGIC2 could not be traced so interactions invovling this protein were not added. david.virshup@duke-nus.edu.sg Developmental cell 2014 Only protein-protein interactions imex curation imex curation yu-2014-6 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Yu J., Chia J., Canning CA., Jones CM., Bard FA., Virshup DM., Yu J., Chia J., Canning CA., Jones CM., Bard FA., Virshup DM. STF cells - an HEK293 cell line that contains an integrated SuperTOPflash reporter Origin of WNT3A inferred from PMID:22784633. Origin of HA-PORCN inferred from PMID:26257057. Source of ERGIC2 could not be traced so interactions invovling this protein were not added. david.virshup@duke-nus.edu.sg Developmental cell 2014 Only protein-protein interactions imex curation imex curation yu-2014-7 human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Yu J., Chia J., Canning CA., Jones CM., Bard FA., Virshup DM., Yu J., Chia J., Canning CA., Jones CM., Bard FA., Virshup DM. Origin of WNT3A inferred from PMID:22784633. Origin of HA-PORCN inferred from PMID:26257057. Source of ERGIC2 could not be traced so interactions invovling this protein were not added. david.virshup@duke-nus.edu.sg Developmental cell 2014 Only protein-protein interactions imex curation imex curation wls_human Protein wntless homolog WLS C1orf139 GPR177 Integral membrane protein GPR177 Protein evenness interrupted homolog Putative NF-kappa-B-activating protein 373 UNQ85/PRO18667 protein protein human Homo sapiens Human MAGAIIENMSTKKLCIVGGILLVFQIIAFLVGGLIAPGPTTAVSYMSVKCVDARKNHHKTKWFVPWGPNHCDKIRDIEEAIPREIEANDIVFSVHIPLPHMEMSPWFQFMLFILQLDIAFKLNNQIRENAEVSMDVSLAYRDDAFAEWTEMAHERVPRKLKCTFTSPKTPEHEGRYYECDVLPFMEIGSVAHKFYLLNIRLPVNEKKKINVGIGEIKDIRLVGIHQNGGFTKVWFAMKTFLTPSIFIIMVWYWRRITMMSRPPVLLEKVIFALGISMTFINIPVEWFSIGFDWTWMLLFGDIRQGIFYAMLLSFWIIFCGEHMMDQHERNHIAGYWKQVGPIAVGSFCLFIFDMCERGVQLTNPFYSIWTTDIGTELAMAFIIVAGICLCLYFLFLCFMVFQVFRNISGKQSSLPAMSKVRRLHYEGLIFRFKFLMLITLACAAMTVIFFIVSQVTEGHWKWGGVTVQVNSAFFTGIYGMWNLYVFALMFLYAPSHKNYGEDQSNGDLGVHSGEELQLTTTITHVDGPTEIYKLTRKEAQE wnt3a_human Protein Wnt-3a WNT3A protein protein human Homo sapiens Human MAPLGYFLLLCSLKQALGSYPIWWSLAVGPQYSSLGSQPILCASIPGLVPKQLRFCRNYVEIMPSVAEGIKIGIQECQHQFRGRRWNCTTVHDSLAIFGPVLDKATRESAFVHAIASAGVAFAVTRSCAEGTAAICGCSSRHQGSPGKGWKWGGCSEDIEFGGMVSREFADARENRPDARSAMNRHNNEAGRQAIASHMHLKCKCHGLSGSCEVKTCWWSQPDFRAIGDFLKDKYDSASEMVVEKHRESRGWVETLRPRYTYFKVPTERDLVYYEASPNFCEPNPETGSFGTRDRTCNVSSHGIDGCDLLCCGRGHNARAERRREKCRCVFHWCCYVSCQECTRVYDVHTCK porcn_mouse Protein-serine O-palmitoleoyltransferase porcupine Porcn Porc Ppn protein protein mouse Mus musculus Mouse MATFSRQEFFQQLLQGCLLPTVQQGLDQIWLLLTICFACRLLWRLGLPSYLKHASTVAGGFFSLYHFFQLHMVWVVLLSLLCYLVLFLCRHSSHRGVFLSVTILIYLLMGEMHMVDTVTWHKMRGAQMIVAMKAVSLGFDLDRGEVGAVPSPVEFMGYLYFVGTIVFGPWISFHSYLQAVQGRPLSRRWLKKVARSLALALLCLVLSTCVGPYLFPYFIPLDGDRLLRNKKRKARGTMVRWLRAYESAVSFHFSNYFVGFLSEATATLAGAGFTEEKDHLEWDLTVSRPLNVELPRSMVEVVTSWNLPMSYWLNNYVFKNALRLGTFSAVLVTYAASALLHGFSFHLAAVLLSLAFITYVEHVLRKRLAQILSACILSKRCLPDCSHRHRLGLGVRALNLLFGALAIFHLSYLGSLFDVDVDDTTEEQGYGMAYTVHKWSELSWASHWVTFGCWIFYRLIG wnt3a-wls-3 3604661 N/A 3604665 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed Q5T9L3:p.Arg536Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false Q5T9L3:p.Lys537Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false Q5T9L3:p.Glu538Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false Q5T9L3:p.Gln540Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false Q5T9L3:p.Glu541Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false N/A 3604666 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed c-terminal v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Supp. Fig. 2A-F PORCN inhibitor C59 (inhibits palmityolation) porcn-wls-2 3604661 N/A 3604665 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3604667 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false Supp Fig. 1H wnt3a-wls-1 3604662 N/A 3604665 unspecified role unspecified role prey prey subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3604666 unspecified role unspecified role bait bait subcellular prep subcellular preparation subcellular prep over-expressed over expressed level over-expressed c-terminal v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Fig. 1H, Supp. Fig. 1G PORCN inhibitor C59 (inhibits palmityolation) wnt3a-wls-2 3604663 N/A 3604665 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed Q5T9L3:p.Tyr425Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false p.Arg536_Glu541delinsLyslysLeuVal mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false N/A 3604666 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed c-terminal v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Fig. 4I PORCN inhibitor C59 (inhibits palmityolation) porcn-wls-1 3604664 N/A 3604665 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3604667 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false proximity proximity close-contact colocalization neighbourhood interaction false false false Fig. 1I