DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ balanarasimha-2014-2 in vitro In vitro fluorescence fluorescence technology fluorescence experimental particp experimental participant identification experimental particp Balanarasimha M., Davis AM., Soman FL., Rider SD., Hostetler HA. heather.hostetler@wright.edu Biochemistry 2014 imex curation Only protein-protein interactions imex curation balanarasimha-2014-3 human-hep_g2 Homo sapiens hepatocellular carcinoma (HCC) cells (p53-wt). hep_g2 human hepatocellular carcinoma (HCC) cells (p53-wt). anti bait coip anti bait coimmunoprecipitation anti bait coip experimental particp experimental participant identification experimental particp Balanarasimha M., Davis AM., Soman FL., Rider SD., Hostetler HA. heather.hostetler@wright.edu Biochemistry 2014 imex curation Only protein-protein interactions imex curation balanarasimha-2014-1 in vitro In vitro cd circular dichroism CD CD cd experimental particp experimental participant identification experimental particp Balanarasimha M., Davis AM., Soman FL., Rider SD., Hostetler HA. heather.hostetler@wright.edu Biochemistry 2014 imex curation Only protein-protein interactions imex curation nr1h3_human Oxysterols receptor LXR-alpha Liver X receptor alpha Nuclear receptor subfamily 1 group H member 3 NR1H3 LXRA protein protein human Homo sapiens Human MSLWLGAPVPDIPPDSAVELWKPGAQDASSQAQGGSSCILREEARMPHSAGGTAGVGLEAAEPTALLTRAEPPSEPTEIRPQKRKKGPAPKMLGNELCSVCGDKASGFHYNVLSCEGCKGFFRRSVIKGAHYICHSGGHCPMDTYMRRKCQECRLRKCRQAGMREECVLSEEQIRLKKLKRQEEEQAHATSLPPRASSPPQILPQLSPEQLGMIEKLVAAQQQCNRRSFSDRLRVTPWPMAPDPHSREARQQRFAHFTELAIVSVQEIVDFAKQLPGFLQLSREDQIALLKTSAIEVMLLETSRRYNPGSESITFLKDFSYNREDFAKAGLQVEFINPIFEFSRAMNELQLNDAEFALLIAISIFSADRPNVQDQLQVERLQHTYVEALHAYVSIHHPHDRLMFPRMLMKLVSLRTLSSVHSEQVFALRLQDKKLPPLLSEIWDVHE ppara_human Peroxisome proliferator-activated receptor alpha PPARA NR1C1 PPAR Nuclear receptor subfamily 1 group C member 1 protein protein human Homo sapiens Human MVDTESPLCPLSPLEAGDLESPLSEEFLQEMGNIQEISQSIGEDSSGSFGFTEYQYLGSCPGSDGSVITDTLSPASSPSSVTYPVVPGSVDESPSGALNIECRICGDKASGYHYGVHACEGCKGFFRRTIRLKLVYDKCDRSCKIQKKNRNKCQYCRFHKCLSVGMSHNAIRFGRMPRSEKAKLKAEILTCEHDIEDSETADLKSLAKRIYEAYLKNFNMNKVKARVILSGKASNNPPFVIHDMETLCMAEKTLVAKLVANGIQNKEAEVRIFHCCQCTSVETVTELTEFAKAIPGFANLDLNDQVTLLKYGVYEAIFAMLSSVMNKDGMLVAYGNGFITREFLKSLRKPFCDIMEPKFDFAMKFNALELDDSDISLFVAAIICCGDRPGLLNVGHIEKMQEGIVHVLRLHLQSNHPDDIFLFPKLLQKMADLRQLVTEHAQLVQIIKKTESDAALHPLLQEIYRDMY nr1h3-ppara-3 3577664 N/A 3577667 weight by comassie molecular weight estimation by coomasie staining weight by comassie predetermined predetermined participant predetermined western blot western blot Immuno blot Immuno blot unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) N/A 3577668 weight by comassie molecular weight estimation by coomasie staining weight by comassie predetermined predetermined participant predetermined western blot western blot Immuno blot Immuno blot unspecified role unspecified role neutral component neutral component purified purified cy5 label cy5 label cy5 label cy5 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Figure 3A, Figure 6 nr1h3-ppara-4 3577664 N/A 3577667 weight by comassie molecular weight estimation by coomasie staining weight by comassie predetermined predetermined participant predetermined western blot western blot Immuno blot Immuno blot unspecified role unspecified role neutral component neutral component purified purified cy3 label cy3 label cy3 label cy3 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 3577668 weight by comassie molecular weight estimation by coomasie staining weight by comassie predetermined predetermined participant predetermined western blot western blot Immuno blot Immuno blot unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Figure 3B ppara-nr1h3-2 3577665 N/A 3577667 predetermined predetermined participant predetermined western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level native native human-hep_g2 Homo sapiens hepatocellular carcinoma (HCC) cells (p53-wt). hep_g2 human hepatocellular carcinoma (HCC) cells (p53-wt). N/A 3577668 predetermined predetermined participant predetermined western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level native native human-hep_g2 Homo sapiens hepatocellular carcinoma (HCC) cells (p53-wt). hep_g2 human hepatocellular carcinoma (HCC) cells (p53-wt). physical association physical association false false false Figure 4, column 1 nr1h3-ppara-5 3577665 N/A 3577667 predetermined predetermined participant predetermined western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level native native human-hep_g2 Homo sapiens hepatocellular carcinoma (HCC) cells (p53-wt). hep_g2 human hepatocellular carcinoma (HCC) cells (p53-wt). N/A 3577668 predetermined predetermined participant predetermined western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level native native human-hep_g2 Homo sapiens hepatocellular carcinoma (HCC) cells (p53-wt). hep_g2 human hepatocellular carcinoma (HCC) cells (p53-wt). physical association physical association false false false Figure 4, column 2 nr1h3-ppara-2 3577666 N/A 3577667 weight by comassie molecular weight estimation by coomasie staining weight by comassie predetermined predetermined participant predetermined western blot western blot Immuno blot Immuno blot unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) N/A 3577668 weight by comassie molecular weight estimation by coomasie staining weight by comassie predetermined predetermined participant predetermined western blot western blot Immuno blot Immuno blot unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Figure 2A,B, FIgure 5, Table 2