IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ cecere-2013-1 caeel-embryo Caenorhabditis elegans embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Cecere G., Hoersch S., Jensen MB., Dixit S., Grishok A. ag2691@columbia.edu Molecular cell 2013 Only protein-protein interactions imex curation imex curation cecere-2013-2 caeel-embryo Caenorhabditis elegans embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. anti tag coip anti tag coimmunoprecipitation anti tag coip ms participant Identification by mass spectrometry ms participant Cecere G., Hoersch S., Jensen MB., Dixit S., Grishok A. ag2691@columbia.edu Molecular cell 2013 Only protein-protein interactions imex curation imex curation dot11_caeel Histone-lysine N-methyltransferase, H3 lysine-79 specific dot-1.1 Histone H3-K79 methyltransferase Y39G10AR.18 protein protein caeel Caenorhabditis elegans MSEADAGARDESPSRTAEEPAAAMRIKEERRSSSVDVVDVGNGELLVLHSIFYQGKTLRLPGNRAHMYPVVFQMIKGVCSLVKQLVVAFPKGWDQNTPSISEIAALTKSFNRVAKPFASNWSGSYNTDTLKEWGEPNCSAEVAKAITTYAYECAVPRPADLNQHYKSFTSETYGETNPEQLISIIDELNIGPQDVFVDLGSGIGQLVCLTAAYAKCKKSVGIELSQVPSNFAQDLAGYFKKFMSHFGKNHGKFEHIQGDFLNPKFKQLICEEATVIFINNFAFDAALMLRINTELLQDLKHGTRIVTTKELGTNKKEITFRSTSDINAISHTTELKTTESAVSWTSSHVKFWLTTIDHTKLIKYYEDQRRRQEVKSSREGSEISDGRDMGLKKRKSQRESSVHPDKLQKTEQAAASSHQSPKWNEPDTDYTPPAKKPKKEKLLREQQDATPASSHHHGASSSSGKDREKEKEKKKNKIYEEKKVKTPKPPKSSSSRYSSETPTSHHHHHRSNSISHSSDVIRPSQPKATAPPPPLVPAPARATASTPPPAPPAARAQSPKREEPLEPPTDLIHHGGGQLDAKTMNALHTIREAATTSAQAAAIQDAINSVLSQPTEASPSAFGPPLAHLPAPVAIYPTPPPPPAPAPAAPQQASAAPAAPNVMPVCTEIAAEQRHTFMIPPTDPFYNMIVSYYFAMKQFCNQSKTADPEFVGRLRLDIEAEEARRAELKESITLTSTQIDELLATGVNTLKSRLDELGMPSVTDVTELLAGSKQIVTQHKGLTNTVAQMENSVAVEEQKLRLIGGPDAVRYFDEAMSHPNVDIAKLTDLVITTRPPNFVAQILLPDDSPTASIDSKVSPSSSSSRRPRQPKPRANNTAAGAGGGGKRGTSGGRKSDGGGGGGATEDVELEIRQFVQHALKVDNAVKEKERKARGNFMAAAADRIPR zfp1_caeel Zinc finger protein zfp-1 zfp-1 Protein AF-10 homolog F54F2.2 protein protein caeel Caenorhabditis elegans MKEMVGGCCVCADENGWTDNPLIYCDGENCEVAVHQGCYGIQEVPEGEWFCAKCTKASAMMPGSINEATFCCQLCPFDYGALKKTDRNGWAHVICALYIPEVRFGNVHSMEPVILNDVPTDKFNKLCYICNEERPNDAKKGACMSCNKSTCKRSFHVTCAQRKGLLCEEGAISRNVKYCGYCENHLKKAINDPAIKVIPACPPVQRLSKEQDKKKTVLLTSLPLPPPAPRLHMLADPLPIKSNKVNNVLGLGSAINAVSLEERPASGSTVNSGIFVPPPTAFSPPLTTSSRSSVAQDPSPPLTINKNSLSSSGPLIPSTAHLSATTASATPIMANGSTLPPSSETTVGTHCLQQLQIQSAAAAAITQNQIGPSELNGYPAASQLSSFMHEIPARNTTSVASLLPPGAAEYHLNGSGDEEKTVKAVLTAPLTKAKRIRDSKNDMMDKTHKRPRANARPPAVLGSMSSGSSGGTVGKSPSMQRLQNLVAPIVSETVTDFQRDRVADRTAAERRAAAAQSQPSTSTNGGPNVTIPAVVEVHTNSTNSTNHQNNGLTQNAPASTSMQAGTSSNDGVISQNGTSSTSQSNRLNLPSFMEQLLERQWDQGSSLLMANAHFDVAQLLSCLFQLKSENFRLEENLSGLRKRRDHLFALNSRLAEVNTLDVSKRQRSDGLLLQQQIAHHLDPTSIVPKAEVHKQEPLSAPTSVPLPANHSSSLFEDIKAPKATYSRKTPSNIPLTVPLSTAATALTTTTAASSGAPVNSNIQNHRATPSTAGAPMAATPIMTAVTSANELAALSPERAQALLNMYRMPLDANVAAQLSMITNFPGQVNPSLFSRLLAVNMMNGGLQPNGQPLSALPPPTSATPNGK hsp90_caeel Heat shock protein 90 daf-21 Abnormal dauer formation protein 21 C47E8.5 protein protein caeel Caenorhabditis elegans MSENAETFAFQAEIAQLMSLIINTFYSNKEIYLRELISNASDALDKIRYQALTEPSELDTGKELFIKITPNKEEKTLTIMDTGIGMTKADLVNNLGTIAKSGTKAFMEALQAGADISMIGQFGVGFYSAFLVADKVVVTSKNNDDDSYQWESSAGGSFVVRPFNDPEVTRGTKIVMHIKEDQIDFLEERKIKEIVKKHSQFIGYPIKLVVEKEREKEVEDEEAVEAKDEEKKEGEVENVADDADKKKTKKIKEKYFEDEELNKTKPIWTRNPDDISNEEYAEFYKSLSNDWEDHLAVKHFSVEGQLEFRALLFVPQRAPFDLFENKKSKNSIKLYVRRVFIMENCEELMPEYLNFIKGVVDSEDLPLNISREMLQQSKILKVIRKNLVKKCMELIDEVAEDKDNFKKFYEQFGKNLKLGIHEDSTNRKKLSDFLRYSTSAGDEPTSLKEYVSRMKENQTQIYYITGESKDVVAASAFVERVKSRGFEVLYMCDPIDEYCVQQLKEYDGKKLVSVTKEGLELPETEEEKKKFEEDKVAYENLCKVIKDILEKKVEKVGVSNRLVSSPCCIVTSEYGWSANMERIMKAQALRDSSTMGYMAAKKHLEINPDHAIMKTLRDRVEVDKNDKTVKDLVVLLFETALLASGFSLEEPQSHASRIYRMIKLGLDIGDDEIEDSAVPSSCTAEAKIEGAEEDASRMEEVD g3p4_caeel Glyceraldehyde-3-phosphate dehydrogenase 4 gpd-4 F33H1.2 protein protein caeel Caenorhabditis elegans MSKANVGINGFGRIGRLVLRAAVEKDTVQVVAVNDPFITIDYMVYLFKYDSTHGQFKGTVTYDGDFLIVQKDGKSSHKIKVFNSKDPAAIAWGSVKADFVVESTGVFTTKEKASAHLQGGAKKVIISAPSADAPMYVVGVNHEKYDASNDHVISNASCTTNCLAPLAKVINDNFGIIEGLMTTVHAVTATQKTVDGPSGKLWRDGRGAGQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTPDVSVVDLTVRLEKPASMDDIKKVVKAAADGPMKGILAYTEDQVVSTDFVSDPHSSIFDTGACISLNPNFVKLVSWYDNEYGYSNRVVDLIGYIATRG o44175_caeel rfc-2 F58F6.4 CELE_F58F6.4 protein protein caeel Caenorhabditis elegans MSKSEKQQLAPWVEKYRPKVLADIVGNENIVERLKVIGHEGNVPNIVLSGPPGCGKTTSVWALARELLGDKVKEAVLELNASDERGIDVVRHRIKTFAQTKVTLPEGRHKIIILDEADSMTDGAQQALRRTMEMYTKTTRFALACNQSEKIIEPIQSRCALLRYTKLSPVQLLTRVKEVAKAEKVNYDDGGLEAILFTAQGDMRQALNNLQATVNAYELVNKENVLKVCDEPHPDLMIKMLHYCTDRKFFEASKIIHEFHRLGFSSDDIVSTLFRVVKTVELSKNVSEQLRMEYIRQIAMCHMRIVQGLTSKLQLSRLIADLCRVSAAATATSA hsp1_caeel Heat shock protein hsp-1 hsp-1 hsp70a F26D10.3 Heat shock 70 kDa protein A protein protein caeel Caenorhabditis elegans MSKHNAVGIDLGTTYSCVGVFMHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPHNTVFDAKRLIGRKFDDPAVQSDMKHWPFKVISAEGAKPKVQVEYKGENKIFTPEEISSMVLLKMKETAEAFLGTTVKDAVVTVPAYFNDSQRQATKDAGAIAGLNVLRIINEPTAAAIAYGLDKKGHGERNVLIFDLGGGTFDVSILTIEDGIFEVKSTAGDTHLGGEDFDNRMVNHFCAEFKRKHKKDLASNPRALRRLRTACERAKRTLSSSSQASIEIDSLFEGIDFYTNITRARFEELCADLFRSTMDPVEKSLRDAKMDKSQVHDIVLVGGSTRIPKVQKLLSDLFSGKELNKSINPDEAVAYGAAVQAAILSGDKSEAVQDLLLLDVAPLSLGIETAGGVMTALIKRNTTIPTKTAQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELSGIPPAPRGVPQIEVTFDIDANGILNVSATDKSTGKQNKITITNDKGRLSKDDIERMVNEAEKYKADDEAQKDRIGAKNGLESYAFNLKQTIEDEKLKDKISPEDKKKIEDKCDEILKWLDSNQTAEKEEFEHQQKDLEGLANPIISKLYQSAGGAPPGAAPGGAAGGAGGPTIEEVD q9n4f3_caeel AP complex subunit beta apb-1 CELE_Y71H2B.10 Y71H2B.10 protein protein caeel Caenorhabditis elegans MTDSKYFTTTKKGEIFELKNELNSDKKEKKKEAVKKVIASMTVGKDVSALFPDVVNCMQTDNVELKKLVYLYLMNYAKSQPDLAIMAVNTFVKDCEDPNPLIRALAVRTMGCIRVEKITEYLCDPLRRCMKDEDPYVRKTAAVCVAKLHDMNPALVKEQGFVELLNDLLSDANPMVVANAVAALTEMNEQQTVIEVNSQMVNKLLTALNECTEWGQVFILDALAGYTPRDERETQNICERISPRLAHANAAVVLSTVKVLMKLIDMMPADSDFITQLTKKLAPPMVTLLSAEPEIQYVALRNINLIVQKRPDILKQEMKVFFVKYNDPIYVKMEKLDIMIRLAQQNNIAQVLSELKEYATEVDVDFVRKSVRAIGRCAIKVEASSERCVQTLLELIQTKVNYVVQEAVVVIKDIFRKYPNRYESIISALCENLDTLDEPEARASMIWIIGEYAERIDNADELLESFVEGFHDENTQVQLQLLTAVVKLFLKRPGVAQALVQRVLSLATQDSDNPDLRDRGFIYWRLLSADPAAAKNVVLTEKPLISEETDLLEPSLLEQLVCHIGTLASVYHKPPSAFIDPAKAPLRQINPIPAAKFGSSLLDTSSTPNAARNGSAAAPTVIPSQDQVIADLLSLDLNAPAASANTGAFGGAMSGTTSMGGLDDLLGLGGDPMPAAPTNSASYNPFDGLGGLGGAAPAAPPSQQPASIGGLAEIFGSGGFGGSSGITYPKELWLDASKAMGMQVEGTFVRRGGKIFMEMTITNRAMQAISGFALQFNKNSFGLIPVEQVNPAPILPNQSQNYTIACDTTGAVQVTTPLTNLQVAIKNDINAFYFATTVPLLAYFREDGQMEKREFLEEWKSIPEQNEQQFTLQNTHNMNADAICTKLQQNNIHTVARRQVDNQQLLYHSVKYTNNLNVLSELKVNSQTTSITLSLKSKNLMAIANINEVFQALLN pde6_caeel Probable 3',5'-cyclic phosphodiesterase pde-6 Y95B8A.10 pde-6 protein protein caeel Caenorhabditis elegans MGDRSGVGGEEGEKSVLGASAHSQAGRRQQHTPAARRGAQRAPAATAAAASRPVFAMRWCCGGSYSENGGGGGGSRGAPPTPLLTVAATSSSTAHDTFGPMQVKMLKTAVIVTEGTGESVLLDSLRASGWICTVSFPTQATSDVESICPLAVFIDLRVPHPSQIAKDVSSVSTEEVLIVSIAEKHISEKRRRALAQSNIIHHVTWNTRDVVLFDYVGRLANRIRALPALFAVLDETDQAVEICDEQRVVQYVNRAYENVTGCIRSEVIGQPESEMRRKSLPRARGEEERRRSCDWKFIRVPFANNSQFVYMKRSNTTGDTAAIFRDVSLKSLKSQTGGIEAPISEVLTMLRDVSARVDGEPAQTIKDAMKVLSSHELYAPSINRFRDADRIATQYYDGLIRLHHPARQRKRSVVDAHREKRGSHGERRRVSADVKNALENDNCWKFDILHLEKVSDHHALSQVGMKVFERWKVCDVLGCSDDLLHRWILSIEAHYHAGNTYHNATHAADVLQATSFFLDSPSVAVHVNESHAVAALLAAAVHDLDHPGRGNAYLINTRQSLAILYNDNSILENHHIALAFQLTLQHNANVNIFSSLSREEFIQMRHAMVEMVLATDISRHFEYLAKFNKMHVTDVPEEQRDTNSLTICDMLVKCADISNPAREWGLCQRWAHRIVEEYFEQTREEKEKGLPVTMEVFDRNTCNVPITQCGFIDMFAREAFATFTEFAKLGELSDQLESNYEKWKVMTSQWTPTHNTNLVL q9xvr7_caeel DNA replication licensing factor MCM3 mcm-3 C25D7.6 CELE_C25D7.6 protein protein caeel Caenorhabditis elegans MDGQADGMFIDRAEDARKQEITEQYLNFLDNQADTFHYRQKIVKMMNDGESRLVMNLDEIREILPERAEGLLTQSVLEMNCLQSALQMAINRSDVQAVVKTGFHVGFEGTFGERHVNPRTLKSSYLGNLVCCEGIVTKCSTVRQKLLTSVHYCPATNKVLEKKFADFTMLDTAVTNNNYPTEDENKNPLETEFGHSVYKDHQTFTIQELPESAPAGQLPRAVDCVADLDLADRVKPGDRVRIIGVFRVLPNKQNGVSSGSFRSIIIINHIQMLSKEVIPNFEPQDVKDVRKISKSRNPFELLARSLAPSICGHEETKKALLCLLLGGMEKILNNGSRLRGDINVLLIGDPSVAKSQLLRYVLRMAPRAITTTGRGSSGVGLTAAVTTDPDSGERRLEAGAMVLADRGVVCIDEFDKMSDIDRTAIHEVMEQGRVTISKAGIHAKLNARCSVLAAANPVYGRYNPFKSPMENIGMQDSLLSRFDLIFVLLDEHDADKDANVAEHVLKLHTYRTQGEADGTVLPMGGGVETISTINMETKKASSSIYEENTQWAGIQDTKILTMDFMRKYIHLARSVQPKLTDEATEYISEVYADIRSFDITKTDQERTMPVTARQLETLIRLSTAIAKARFSKKVEREDTEKAYNLLHFACFKEKPKARQEYEKKKRAPRIDGDDDEDQSDNEDMATSEEPQSSSVPTAATPRRGVRRRAEDDDSQDSMMDTTIGESQPTAKKARTGPAAIGVDRYKELRKYVRKAFDDIGSTDDMVELQIITDSVQTQAGSHKFTEDELKAGYEQLENDNAAMIAEDKITLI o45865_caeel ADP/ATP translocase ant-1.1 ADP,ATP carrier protein CELE_T27E9.1 T27E9.1 protein protein caeel Caenorhabditis elegans MSKEKSFDTKKFLIDLASGGTAAAVSKTAVAPIERVKLLLQVQDASKAIAVDKRYKGIMDVLIRVPKEQGVAALWRGNLANVIRYFPTQAMNFAFKDTYKAIFLEGLDKKKDFWKFFAGNLASGGAAGATSLCFVYPLDFARTRLAADIGKANDREFKGLADCLIKIVKSDGPIGLYRGFFVSVQGIIIYRAAYFGMFDTAKMVFASDGQKLNFFAAWGIAQVVTVGSGILSYPWDTVRRRMMMQSGRKDILYKNTLDCAKKIIQNEGMSAMFKGALSNVFRGTGGALVLAIYDEIQKFL ym2a_caeel-1 3575172 N/A 3575174 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3575175 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 1A zfp_1-dot_1.1-1 3575172 N/A 3575174 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3575175 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false Supp. Fig. 1B ym2a_caeel-ant_1.1-1 3575173 N/A 3575176 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3575177 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3575178 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3575179 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3575180 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3575181 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3575182 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3575183 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3575174 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3575175 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Supp. Table 1, Supp. figure 1A.