UniProt UniProt http://www.uniprot.org hagiwara-2011-1 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Hagiwara K., Nagamori S., Umemura YM., Ohgaki R., Tanaka H., Murata D., Nakagomi S., Nomura KH., Kage-Nakadai E., Mitani S., Nomura K., Kanai Y. ykanai@pharma1.med.osaka-u.ac.jp PloS one 2011 Only protein-protein interactions imex curation imex curation hagiwara-2011-2 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Hagiwara K., Nagamori S., Umemura YM., Ohgaki R., Tanaka H., Murata D., Nakagomi S., Nomura KH., Kage-Nakadai E., Mitani S., Nomura K., Kanai Y. ykanai@pharma1.med.osaka-u.ac.jp PloS one 2011 Only protein-protein interactions imex curation imex curation hagiwara-2011-3 caeel Caenorhabditis elegans fluorescence imaging fluorescence microscopy fluorescence imaging immunohistochemistry immunohistochemistry Hagiwara K., Nagamori S., Umemura YM., Ohgaki R., Tanaka H., Murata D., Nakagomi S., Nomura KH., Kage-Nakadai E., Mitani S., Nomura K., Kanai Y. ykanai@pharma1.med.osaka-u.ac.jp PloS one 2011 Only protein-protein interactions imex curation imex curation hagiwara-2011-4 caeel Caenorhabditis elegans anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Hagiwara K., Nagamori S., Umemura YM., Ohgaki R., Tanaka H., Murata D., Nakagomi S., Nomura KH., Kage-Nakadai E., Mitani S., Nomura K., Kanai Y. ykanai@pharma1.med.osaka-u.ac.jp PloS one 2011 Only protein-protein interactions imex curation imex curation aat6_caeel Amino acid transporter protein 6 aat-6 T11F9.4 protein protein caeel Caenorhabditis elegans MLNVFGVSASMPDDSRSQKMGLLGAISYIVGNIVGSGIFITPTSIIENVNSVGLSLAIWILAAFISMLGSFCYVELGTSIRLSGGDFAYLCFMKWYPVAFAFMCIGCTINYPATLAVQAQTFAEYVFRGAGVELDETSEFWAKKLLGFSLIILLMFMNFFSLKTFVQRFSILASLAKIAATLLIIITGFYYLIFKHWKQNLEEPFKGSNWNPGPFVNALFAGLFSYDGWDILNFGAEEIENPKRTMPLSIIIGMTCIGVIYVAVNVAYSIVLSPTEMIASNAVAIDFANKTLGAAAFVVPVMVAILLIGSLNSTMFSASRYLQAVSRQGHIPSAISGIAPNCDSPRVALLVHILIAIAVSFLGDPDKLINYVAFAQWSQRAFTMSALLYLRIRGRPRHPDRIQLPIIMPILFFLVCTSMVVISIIDDFKSSAVGLGILLGGLIIFIIFVWDRALPSSHTFRNATHVINEESTKFMQIIFNVVPERVGDEEMKNAIGGAESESEKVPAYKISPTGNGQFKCTRM g5edm4-1 Na(+)/H(+) exchange regulatory cofactor-like protein nrfl-1 Regulatory cofactor of Na(+)/H(+) exchanger Sodium-hydrogen exchanger regulatory factor 1 nrfl-1 mpz-2 tag-60 C01F6.6 protein protein caeel Caenorhabditis elegans MVHIPSDVTPPRLCVVEKLNGENEYGYNLHAEKGRGQFVGTVDPDSPAERGGLITGDRIFAVNGHSIIGENHKKVVERIKANPNRCEMLVISEEGAKWYNENNVQITLDLPNIERVSPMSKETPVFVPPPPPPTDAMPYLPRLAELNKGTPDQEFGFNLHAERGRGHFIGTVDAGGIGEKAGLEAGQRIVGVNGQLIYPTTGHKEVVALIKKDTMKTTLLVASEDVDKYHKDHNIAYSWDNVERVDTRPVINVETHHHHEEVSVPKSNGYDVPPLNPHSIQVNEEREISKMTTTTRTETITNSNSAYQYKESSTAYDAYATPPVDSNDLMDEVFGRVNLPGVTMSSHTEVLPPTDDISSVSSLSSHRESAVDVPVSHQYVPSYATESHQKHEQHSQTHHHHHQHQQPSPLSNGSSHGYAASSTSGYDDDDIYHLSAREARERLRMKNRKHHLHEMSLNEKYQLVSNM g5edm4-2 Na(+)/H(+) exchange regulatory cofactor-like protein nrfl-1 Regulatory cofactor of Na(+)/H(+) exchanger Sodium-hydrogen exchanger regulatory factor 1 nrfl-1 mpz-2 tag-60 C01F6.6 protein protein caeel Caenorhabditis elegans MDRNPSEPERIDWKNPITWISYLYTSFVYYLWLISSHLPHLPQPQRITDDKSTEQITRDAGIPRTEIVTRGDATQTVRQVAGLGDPAPKDSSLLPPSSSFVYCNNCLTPHSSQRTCSPAYVSTLSRQKSLTLLKKLKKPSSSASSTTTTTSSTVSFRAPSRASSTISDASTIRPSELIAIVPSGSHVLLRVPKLDWKTESIRIKRTRMSDWMDAEQRDVMRQFDEVIRSEEEDERTSRKRNGIFKDGSVRSIESLESRSRSKETPVFVPPPPPPTDAMPYLPRLAELNKGTPDQEFGFNLHAERGRGHFIGTVDAGGIGEKAGLEAGQRIVGVNGQLIYPTTGHKEVVALIKKDTMKTTLLVASEDVDKYHKDHNIAYSWDNVERVDTRPVINVETHHHHEEVSVPKSNGYDVPPLNPHSIQVNEEREISKMTTTTRTETITNSNSAYQYKESSTAYDAYATPPVDSNDLMDEVFGRVNLPGVTMSSHTEVLPPTDDISSVSSLSSHRESAVDVPVSHQYVPSYATESHQKHEQHSQTHHHHHQHQQPSPLSNGSSHGYAASSTSGYDDDDIYHLSAREARERLRMKNRKHHLHEMSLNEKYQLVSNM nhrf1_caeel Na(+)/H(+) exchange regulatory cofactor-like protein nrfl-1 nrfl-1 Regulatory cofactor of Na(+)/H(+) exchanger Sodium-hydrogen exchanger regulatory factor 1 mpz-2 tag-60 C01F6.6 protein protein caeel Caenorhabditis elegans MVHIPSDVTPPRLCVVEKLNGENEYGYNLHAEKGRGQFVGTVDPDSPAERGGLITGDRIFAVNGHSIIGENHKKVVERIKANPNRCEMLVISEEGAKWYNENNVQITLDLPNIERVSPMSKETPVFVPPPPPPTDAMPYLPRLAELNKGTPDQEFGFNLHAERGRGHFIGTVDAGGIGEKAGLEAGQRIVGVNGQLIYPTTGHKEVVALIKKDTMKTTLLVASEDVDKYHKDHNIAYSWDNVERVDTRPVINVETHHHHEEVSVPKSNGYDVPPLNPHSIQVNEEREISKMTTTTRTETITNSNSAYQYKESSTAYDAYATPPVDSNDLMDEVFGRVNLPGVTMSSHTEVLPPTDDISSVSSLSSHRESAVDVPVSHQYVPSYATESHQKHEQHSQTHHHHHQHQQPSPLSNGSSHGYAASSTSGYDDDDIYHLSAREARERLRMKNRKHHLHEMSLNEKYQLVSNM aat_6-nrfl_1-1 3578635 N/A 3578639 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false N/A 3578640 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association false false false Table 1, Fig. 2A aat_6-nrfl_1-3 3578635 N/A 3578639 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 3578641 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association false false false Table 1 aat_6-nrfl_1-2 3578636 N/A 3578639 unspecified role unspecified role bait bait purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 3578640 unspecified role unspecified role prey prey purified purified region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false G5EDM4-1:p.Gly26_Tyr27delinsAlaAla mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false G5EDM4-1:p.[Gly26_Tyr27delinsAlaAla;Glu154_Phe155delinsAlaAla] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false G5EDM4-1:p.Glu154_Phe155delinsAlaAla mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig. 2B, 2C aat_6-nrfl_1-4 3578637 n/a 3578642 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3578639 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag certain certain sequence position certain certain certain sequence position certain false colocalization colocalization false false false Fig. 4B aat_6-nrfl_1-5 3578638 n/a 3578642 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3578639 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 4D