IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ geng-2010-1 in vitro In vitro emsa electrophoretic mobility shift assay Gel retardation assay band shift band shift Gel retardation assay emsa weight by staining molecular weight estimation by staining weight by staining Geng H., Law PP., Ng MC., Li T., Liang LY., Ge TF., Wong KB., Liang C., Ma RC., So WY., Chan JC., Ho YY. yyh2@columbia.edu PloS one 2010 Only protein-protein interactions imex curation imex curation geng-2010-2 in vitro In vitro emsa supershift electrophoretic mobility supershift assay emsa supershift weight by staining molecular weight estimation by staining weight by staining Geng H., Law PP., Ng MC., Li T., Liang LY., Ge TF., Wong KB., Liang C., Ma RC., So WY., Chan JC., Ho YY. yyh2@columbia.edu PloS one 2010 Only protein-protein interactions imex curation imex curation geng-2010-3 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Geng H., Law PP., Ng MC., Li T., Liang LY., Ge TF., Wong KB., Liang C., Ma RC., So WY., Chan JC., Ho YY. yyh2@columbia.edu PloS one 2010 Only protein-protein interactions imex curation imex curation geng-2010-4 human-wrl68 human WRL68 cell human-wrl68 human wrl68 cell Hela cells containing human papilloma viral (HPV) DNA sequences. uterus Uterus ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Geng H., Law PP., Ng MC., Li T., Liang LY., Ge TF., Wong KB., Liang C., Ma RC., So WY., Chan JC., Ho YY. yyh2@columbia.edu PloS one 2010 Only protein-protein interactions imex curation imex curation geng-2010-5 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 1 hybrid one hybrid yeast one-hybrid yeast one hybrid one-hybrid 1 hybrid yeast one-hybrid one-hybrid yeast one hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Geng H., Law PP., Ng MC., Li T., Liang LY., Ge TF., Wong KB., Liang C., Ma RC., So WY., Chan JC., Ho YY. yyh2@columbia.edu human brain cDNA library PloS one 2010 Only protein-protein interactions imex curation imex curation apoe_promoter human_apoe_promoter nucleic acid nucleic acid human Homo sapiens Human CAAGGTCACACAGCTGGCAACTGGCAGAGCCAGGATTCACGCCCTGGCAATTTGACTCCAGAATCCTAACCTTAACCCAGAAGCACGGCTTCAAGCCCCTGGAAACCACAATACCTGTGGCAGCCAGGGGGAGGTGCTGGAATCTCATTTCACATGTGGGGAGGGGGCTCCCCTGTGCTCAAGGTCACAACCAAAGAGGAAGCTGTGATTAAAACCCAGGTCCCATTTGCAAAGCCTCGACTTTTAGCAGGTGCATCATACTGTTCCCACCCCTCCCATCCCACTTCTGTCCAGCCGCCTAGCCCCACTTTCTTTTTTTTCTTTTTTTGAGACAGTCTCCCTCTTGCTGAGGCTGGAGTGCAGTGGCGAGATCTCGGCTCACTGTAACCTCCGCCTCCCGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCAAGTAGCTAGGATTACAGGCGCCCGCCACCACGCCTGGCTAACTTTTGTATTTTTAGTAGAGATGGGGTTTCACCATGTTGGCCAGGCTGGTCTCAAACTCCTGACCTTAAGTGATTCGCCCACTGTGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCTACCGCCCCCAGCCCCTCCCATCCCACTTCTGTCCAGCCCCCTAGCCCTACTTTCTTTCTGGGATCCAGGAGTCCAGATCCCCAGCCCCCTCTCCAGATTACATTCATCCAGGCACAGGAAAGGACAGGGTCAGGAAAGGAGGACTCTGGGCGGCAGCCTCCACATTCCCCTTCCACGCTTGGCCCCCAGAATGGAGGAGGGTGTCTGTATTACTGGGCGAGGTGTCCTCCCTTCCTGGGGACTGTGGGGGGTGGTCAAAAGACCTCTATGCCCCACCTCCTTCCTCCCTCTGCCCTGCTGTGCCTGGGGCAGGGGGAGAACAGCCCACCTCGTGACTGGGGGCTGGCCCAGCCCGCCCTATCCCTGGGGGAGGGGGCGGGACAGGGGGAGCCCTATAATTGGACAAGTCTGGGATCCTTGAGTCCTACTCAGCCCCAGCGGAGGTGAAGGACGTCCTTCCCCAGGAGCCGGTGAGAAGCGCAGTCGGGGGCACGGGGATGAGCTCAGGGGCCTCTAGAAAGAGCTGGGACCCTGGGAAGCCCTGGCCTCCAGGTAGTCTCAGGAGAGCTACTCGGGGTCGGGCTTGGGGAGAGGAGGAGCGGGGGTGAGGCAAGCAGCAGGGGACTGGACCTGGGAAGGGCTGGGCAGCAGAGACGACCCGACCCGCTAGAAGGTGGGGTGGGGAGAGCAGCTGGACTGGGATGTAAGCCATAGCAGGACTCCACGAGTTGTCACTATCATTTATCGAGCACCTACTGGGTGTCCCCAGTGTCCTCAGATCTCCATAACTGGGGAGCCAGGGGCAGCGACACGGTAGCTAGCCGTCGATTGGA atf4_human Cyclic AMP-dependent transcription factor ATF-4 Activating transcription factor 4 CREB2 ATF4 TXREB Cyclic AMP-responsive element-binding protein 2 Tax-responsive enhancer element-binding protein 67 protein protein human Homo sapiens Human MTEMSFLSSEVLVGDLMSPFDQSGLGAEESLGLLDDYLEVAKHFKPHGFSSDKAKAGSSEWLAVDGLVSPSNNSKEDAFSGTDWMLEKMDLKEFDLDALLGIDDLETMPDDLLTTLDDTCDLFAPLVQETNKQPPQTVNPIGHLPESLTKPDQVAPFTFLQPLPLSPGVLSSTPDHSFSLELGSEVDITEGDRKPDYTAYVAMIPQCIKEEDTPSDNDSGICMSPESYLGSPQHSPSTRGSPNRSLPSPGVLCGSARPKPYDPPGEKMVAAKVKGEKLDKKLKKMEQNKTAATRYRQKKRAEQEALTGECKELEKKNEALKERADSLAKEIQYLKDLIEEVRKARGKKRVP 3BBB7379DC3B0D07 No Uniprot Update and Caution removed " The protein could not be merged with EBI-12907955 because of some conflicts with the protein sequence (features which cannot be shifted)." -EBI-12907955 no longer exists. apoe_human_gene Apolipoprotein E APOE gene gene human Homo sapiens Human apoe-atf4-1 3551571 N/A 3551576 unspecified role unspecified role bait bait purified purified g.529A>T mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false region fluorescent dye fluorescent dye label fluorescent dye undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false 5'-GTTTCACCATGTTGGCCAGGCTGGTCTCAAACTCCT GACCTTAAGTGATTCGCCCACTGTG-3' promoter region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3551577 unspecified role unspecified role prey prey purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Figure 2a apoe-atf4-3 3551572 N/A 3551576 unspecified role unspecified role bait bait purified purified g.529A>T mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false region fluorescent dye fluorescent dye label fluorescent dye undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false promoter region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3551577 unspecified role unspecified role prey prey purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Figure 2b apoe-atf4-2 3551573 N/A 3551577 unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3551578 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level promoter region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Forward: TTCAAGCGATTCTCCTGCCT Reverse: TGGGGATCTGGACTCCTGGA physical association physical association false false false Figure 2c atf4-apoe-1 3551574 N/A 3551577 unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3551578 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level promoter region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Forward:TTCAAGCGATTCTCCTGCCT Reverse: TGGGGATCTGGACTCCTGGA physical association physical association false false false Figure 2c apoe-atf4-4 3551575 N/A 3551577 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell N/A 3551576 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell g.529A>T mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false promoter region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 5'-CTGGTCTCAAACTCCTGACCT-3' physical association physical association false false false NA