UniProt UniProt http://www.uniprot.org lee-2009-55 human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lee MH., Lin SR., Chang JY., Schultz L., Heath J., Hsu LJ., Kuo YM., Hong Q., Chiang MF., Gong CX., Sze CI., Chang NS. Control interactions could not be added as source of bait proteins was not given Source of TIAP originally described in PMID:9918798 and in subsequent publications by the corresponding author on this paper changns@mail.ncku.edu.tw Mouse L929 fibroblast cDNA Cell death & disease 2009 Only protein-protein interactions imex curation imex curation lee-2009-48 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Lee MH., Lin SR., Chang JY., Schultz L., Heath J., Hsu LJ., Kuo YM., Hong Q., Chiang MF., Gong CX., Sze CI., Chang NS. Control interactions could not be added as source of bait proteins was not given Source of TIAP originally described in PMID:9918798 and in subsequent publications by the corresponding author on this paper changns@mail.ncku.edu.tw Mouse L929 fibroblast cDNA Cell death & disease 2009 Only protein-protein interactions imex curation imex curation lee-2009-49 chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lee MH., Lin SR., Chang JY., Schultz L., Heath J., Hsu LJ., Kuo YM., Hong Q., Chiang MF., Gong CX., Sze CI., Chang NS. Control interactions could not be added as source of bait proteins was not given Source of TIAP originally described in PMID:9918798 and in subsequent publications by the corresponding author on this paper changns@mail.ncku.edu.tw Mouse L929 fibroblast cDNA Cell death & disease 2009 Only protein-protein interactions imex curation imex curation lee-2009-50 chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Lee MH., Lin SR., Chang JY., Schultz L., Heath J., Hsu LJ., Kuo YM., Hong Q., Chiang MF., Gong CX., Sze CI., Chang NS. Control interactions could not be added as source of bait proteins was not given Source of TIAP originally described in PMID:9918798 and in subsequent publications by the corresponding author on this paper changns@mail.ncku.edu.tw Mouse L929 fibroblast cDNA Cell death & disease 2009 Only protein-protein interactions imex curation imex curation lee-2009-51 musvi-mv1lu Mustela vison mink lung epithelial cells Neovison vison mv1lu Mv1Lu mink lung epithelial fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret tag fluorescence tag visualisation by fluorescence tag fluorescence Lee MH., Lin SR., Chang JY., Schultz L., Heath J., Hsu LJ., Kuo YM., Hong Q., Chiang MF., Gong CX., Sze CI., Chang NS. Control interactions could not be added as source of bait proteins was not given Source of TIAP originally described in PMID:9918798 and in subsequent publications by the corresponding author on this paper changns@mail.ncku.edu.tw Mouse L929 fibroblast cDNA Cell death & disease 2009 Only protein-protein interactions imex curation imex curation lee-2009-52 human-hct116 Human colon carcinoma HCT116 cells hct116 Human colon carcinoma line HCT116 fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret tag fluorescence tag visualisation by fluorescence tag fluorescence Lee MH., Lin SR., Chang JY., Schultz L., Heath J., Hsu LJ., Kuo YM., Hong Q., Chiang MF., Gong CX., Sze CI., Chang NS. Control interactions could not be added as source of bait proteins was not given Source of TIAP originally described in PMID:9918798 and in subsequent publications by the corresponding author on this paper changns@mail.ncku.edu.tw Mouse L929 fibroblast cDNA Cell death & disease 2009 Only protein-protein interactions imex curation imex curation lee-2009-53 human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret tag fluorescence tag visualisation by fluorescence tag fluorescence Lee MH., Lin SR., Chang JY., Schultz L., Heath J., Hsu LJ., Kuo YM., Hong Q., Chiang MF., Gong CX., Sze CI., Chang NS. Control interactions could not be added as source of bait proteins was not given Source of TIAP originally described in PMID:9918798 and in subsequent publications by the corresponding author on this paper changns@mail.ncku.edu.tw Mouse L929 fibroblast cDNA Cell death & disease 2009 Only protein-protein interactions imex curation imex curation lee-2009-54 human-sk-n-sh Homo sapiens, SK-N-SH Caucasian Neuroblastoma Cell Line sk-n-sh sk-n-sh fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret tag fluorescence tag visualisation by fluorescence tag fluorescence Lee MH., Lin SR., Chang JY., Schultz L., Heath J., Hsu LJ., Kuo YM., Hong Q., Chiang MF., Gong CX., Sze CI., Chang NS. Control interactions could not be added as source of bait proteins was not given Source of TIAP originally described in PMID:9918798 and in subsequent publications by the corresponding author on this paper changns@mail.ncku.edu.tw Mouse L929 fibroblast cDNA Cell death & disease 2009 Only protein-protein interactions imex curation imex curation lee-2009-56 human-brain-hippocampus Homo sapiens hippocampus brain_hippocampus brain hippocampus confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Lee MH., Lin SR., Chang JY., Schultz L., Heath J., Hsu LJ., Kuo YM., Hong Q., Chiang MF., Gong CX., Sze CI., Chang NS. Control interactions could not be added as source of bait proteins was not given Source of TIAP originally described in PMID:9918798 and in subsequent publications by the corresponding author on this paper changns@mail.ncku.edu.tw Mouse L929 fibroblast cDNA Cell death & disease 2009 Only protein-protein interactions imex curation imex curation lee-2009-57 mouse-l-929 Mus musculus L929 fibroblasts l-929 L-929 fibroblasts anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lee MH., Lin SR., Chang JY., Schultz L., Heath J., Hsu LJ., Kuo YM., Hong Q., Chiang MF., Gong CX., Sze CI., Chang NS. Control interactions could not be added as source of bait proteins was not given Source of TIAP originally described in PMID:9918798 and in subsequent publications by the corresponding author on this paper changns@mail.ncku.edu.tw Mouse L929 fibroblast cDNA Cell death & disease 2009 Only protein-protein interactions imex curation imex curation lee-2009-58 mouse-b16f10 mouse B16F10 melanoma cell line Mouse b16-f10 B16F10 melanoma cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lee MH., Lin SR., Chang JY., Schultz L., Heath J., Hsu LJ., Kuo YM., Hong Q., Chiang MF., Gong CX., Sze CI., Chang NS. Control interactions could not be added as source of bait proteins was not given Source of TIAP originally described in PMID:9918798 and in subsequent publications by the corresponding author on this paper changns@mail.ncku.edu.tw Mouse L929 fibroblast cDNA Cell death & disease 2009 Only protein-protein interactions imex curation imex curation p05067-pro_0000000092 Amyloid-beta protein 42 Alzheimer disease amyloid protein Cerebral vascular amyloid peptide APP A4 AD1 ABPP PreA4 Protease nexin-II APPI Amyloid precursor protein Amyloid-beta A4 protein Alzheimer disease amyloid A4 protein homolog Amyloid-beta (A4) precursor protein protein protein human Homo sapiens Human DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA 672 713 a4_human Amyloid-beta precursor protein APP A4 AD1 Alzheimer disease amyloid protein ABPP PreA4 Cerebral vascular amyloid peptide Protease nexin-II APPI Amyloid precursor protein Amyloid-beta A4 protein Alzheimer disease amyloid A4 protein homolog Amyloid-beta (A4) precursor protein protein protein human Homo sapiens Human MLPGLALLLLAAWTARALEVPTDGNAGLLAEPQIAMFCGRLNMHMNVQNGKWDSDPSGTKTCIDTKEGILQYCQEVYPELQITNVVEANQPVTIQNWCKRGRKQCKTHPHFVIPYRCLVGEFVSDALLVPDKCKFLHQERMDVCETHLHWHTVAKETCSEKSTNLHDYGMLLPCGIDKFRGVEFVCCPLAEESDNVDSADAEEDDSDVWWGGADTDYADGSEDKVVEVAEEEEVAEVEEEEADDDEDDEDGDEVEEEAEEPYEEATERTTSIATTTTTTTESVEEVVREVCSEQAETGPCRAMISRWYFDVTEGKCAPFFYGGCGGNRNNFDTEEYCMAVCGSAMSQSLLKTTQEPLARDPVKLPTTAASTPDAVDKYLETPGDENEHAHFQKAKERLEAKHRERMSQVMREWEEAERQAKNLPKADKKAVIQHFQEKVESLEQEAANERQQLVETHMARVEAMLNDRRRLALENYITALQAVPPRPRHVFNMLKKYVRAEQKDRQHTLKHFEHVRMVDPKKAAQIRSQVMTHLRVIYERMNQSLSLLYNVPAVAEEIQDEVDELLQKEQNYSDDVLANMISEPRISYGNDALMPSLTETKTTVELLPVNGEFSLDDLQPWHSFGADSVPANTENEVEPVDARPAADRGLTTRPGSGLTNIKTEEISEVKMDAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIATVIVITLVMLKKKQYTSIHHGVVEVDAAVTPEERHLSKMQQNGYENPTYKFFEQMQN tiaf1_human TGFB1-induced anti-apoptotic factor 1 TIAF1 12 kDa TGF-beta-1-induced antiapoptotic factor protein protein human Homo sapiens Human MSSPSSPFREQSFLCAAGDAGEESRVQVLKNEVRRGSPVLLGWVEQAYADKCVCGPSAPPAPTPPSLSQRVMCNDLFKVNPFQLQQFRADPSTASLLLCPGGLDHKLNLRGKAWG smad4_mouse Mothers against decapentaplegic homolog 4 Smad4 Dpc4 Madh4 Deletion target in pancreatic carcinoma 4 homolog SMAD family member 4 protein protein mouse Mus musculus Mouse MDNMSITNTPTSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQYAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLQSNAPSMLVKDEYVHDFEGQPSLPTEGHSIQTIQHPPSNRASTETYSAPALLAPAESNATSTTNFPNIPVASTSQPASILAGSHSEGLLQIASGPQPGQQQNGFTAQPATYHHNSTTTWTGSRTAPYTPNLPHHQNGHLQHHPPMPPHPGHYWPVHNELAFQPPISNHPAPEYWCSIAYFEMDVQVGETFKVPSSCPVVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLSAAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPQPLD smad4_chlae_protein protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey Ortholog of Q13485 tiaf1_chlae_protein protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey Ortholog of O95411 a0a1i9gfb9_mouse Tiaf1 protein protein mouse Mus musculus Mouse MSSPSSPFREQSFLCAAGDAGEESRVQVLKNEVRRGSPVLLGWVEQAYADKCVCGPSAPPAPTPPSLSQRVMCNDLFKVNPFQLQQFRADPSTASLLLCPGGLDHKLNLRGKAWG a4_mouse Amyloid-beta precursor protein App Alzheimer disease amyloid A4 protein homolog ABPP Amyloidogenic glycoprotein Amyloid precursor protein A4 AD1 Alzheimer disease amyloid protein Amyloid-beta (A4) precursor protein Amyloid-beta A4 protein protein protein mouse Mus musculus Mouse MLPSLALLLLAAWTVRALEVPTDGNAGLLAEPQIAMFCGKLNMHMNVQNGKWESDPSGTKTCIGTKEGILQYCQEVYPELQITNVVEANQPVTIQNWCKRGRKQCKTHTHIVIPYRCLVGEFVSDALLVPDKCKFLHQERMDVCETHLHWHTVAKETCSEKSTNLHDYGMLLPCGIDKFRGVEFVCCPLAEESDSVDSADAEEDDSDVWWGGADTDYADGGEDKVVEVAEEEEVADVEEEEADDDEDVEDGDEVEEEAEEPYEEATERTTSTATTTTTTTESVEEVVREVCSEQAETGPCRAMISRWYFDVTEGKCVPFFYGGCGGNRNNFDTEEYCMAVCGSVSTQSLLKTTSEPLPQDPDKLPTTAASTPDAVDKYLETPGDENEHAHFQKAKERLEAKHRERMSQVMREWEEAERQAKNLPKADKKAVIQHFQEKVESLEQEAANERQQLVETHMARVEAMLNDRRRLALENYITALQAVPPRPHHVFNMLKKYVRAEQKDRQHTLKHFEHVRMVDPKKAAQIRSQVMTHLRVIYERMNQSLSLLYNVPAVAEEIQDEVDELLQKEQNYSDDVLANMISEPRISYGNDALMPSLTETKTTVELLPVNGEFSLDDLQPWHPFGVDSVPANTENEVEPVDARPAADRGLTTRPGSGLTNIKTEEISEVKMDAEFGHDSGFEVRHQKLVFFAEDVGSNKGAIIGLMVGGVVIATVIVITLVMLKKKQYTSIHHGVVEVDAAVTPEERHLSKMQQNGYENPTYKFFEQMQN tiaf1-app-1 3534973 N/A 3534984 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3534985 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level thr-743 opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt certain certain sequence position certain certain certain sequence position certain false N/A 3534986 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level association association false false false Fig. 7E tiaf1-app-2 3534973 N/A 3534984 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3534986 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false Fig. 7E TGFB1 tiaf1-smad4-1 3534974 N/A 3534987 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 3534986 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association false false false Fig. 1A smad4_chlae_protein-tiaf1_chlae_protein-1 3534975 N/A 3534988 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3534989 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false TGFB1 Fig. 1B smad4-tiaf1_chlae_protein-1 3534976 N/A 3534989 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3534987 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false Fig. 1D tiaf1-tiaf1-1 3534977 N/A 3534986 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed region eyfp enhanced yellow fluorescent protein tag eyfp undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3534986 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed region cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false proximity proximity close-contact colocalization neighbourhood interaction false false false Fig. 2A tiaf1-1 3534978 N/A 3534986 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed region eyfp enhanced yellow fluorescent protein tag eyfp undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3534986 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed region cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false proximity proximity close-contact colocalization neighbourhood interaction false false false Fig. 2C TGFB1 SMAD4 (P97471) smad4-tiaf1-1 3534979 N/A 3534986 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed region eyfp enhanced yellow fluorescent protein tag eyfp undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3534987 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed region cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false proximity proximity close-contact colocalization neighbourhood interaction false false false Fig. 3C, 3D TGFB1 tiaf1-2 3534980 N/A 3534986 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed region eyfp enhanced yellow fluorescent protein tag eyfp undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3534986 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed region cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false proximity proximity close-contact colocalization neighbourhood interaction false false false Fig. 7B-D TGFB1 SMAD4 (P97471) tiaf1-app-3 3534981 N/A 3534984 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3534986 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level colocalization colocalization false false false Supp. Fig. 10 tiaf1-app-4 3534982 N/A 3534990 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3534991 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false Fig. 7F TGFB2 tiaf1-app-5 3534983 N/A 3534990 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3534991 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false Fig. 7G TGFB1