IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ khanna-2009-2 bovin-retina Bovine retina retina retinal tissue anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Khanna H., Davis EE., Murga-Zamalloa CA., Estrada-Cuzcano A., Lopez I., den Hollander AI., Zonneveld MN., Othman MI., Waseem N., Chakarova CF., Maubaret C., Diaz-Font A., MacDonald I., Muzny DM., Wheeler DA., Morgan M., Lewis LR., Logan CV., Tan PL., Beer MA., Inglehearn CF., Lewis RA., Jacobson SG., Bergmann C., Beales PL., Attié-Bitach T., Johnson CA., Otto EA., Bhattacharya SS., Hildebrandt F., Gibbs RA., Koenekoop RK., Swaroop A., Katsanis N. swaroopa@nei.nih.gov,katsanis@jhmi.edu Nature genetics 2009 Only protein-protein interactions imex curation imex curation khanna-2009-1 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Khanna H., Davis EE., Murga-Zamalloa CA., Estrada-Cuzcano A., Lopez I., den Hollander AI., Zonneveld MN., Othman MI., Waseem N., Chakarova CF., Maubaret C., Diaz-Font A., MacDonald I., Muzny DM., Wheeler DA., Morgan M., Lewis LR., Logan CV., Tan PL., Beer MA., Inglehearn CF., Lewis RA., Jacobson SG., Bergmann C., Beales PL., Attié-Bitach T., Johnson CA., Otto EA., Bhattacharya SS., Hildebrandt F., Gibbs RA., Koenekoop RK., Swaroop A., Katsanis N. swaroopa@nei.nih.gov,katsanis@jhmi.edu adult human retinal cDNA library Nature genetics 2009 Only protein-protein interactions imex curation imex curation khanna-2009-3 chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Khanna H., Davis EE., Murga-Zamalloa CA., Estrada-Cuzcano A., Lopez I., den Hollander AI., Zonneveld MN., Othman MI., Waseem N., Chakarova CF., Maubaret C., Diaz-Font A., MacDonald I., Muzny DM., Wheeler DA., Morgan M., Lewis LR., Logan CV., Tan PL., Beer MA., Inglehearn CF., Lewis RA., Jacobson SG., Bergmann C., Beales PL., Attié-Bitach T., Johnson CA., Otto EA., Bhattacharya SS., Hildebrandt F., Gibbs RA., Koenekoop RK., Swaroop A., Katsanis N. swaroopa@nei.nih.gov,katsanis@jhmi.edu Nature genetics 2009 Only protein-protein interactions imex curation imex curation khanna-2009-4 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Khanna H., Davis EE., Murga-Zamalloa CA., Estrada-Cuzcano A., Lopez I., den Hollander AI., Zonneveld MN., Othman MI., Waseem N., Chakarova CF., Maubaret C., Diaz-Font A., MacDonald I., Muzny DM., Wheeler DA., Morgan M., Lewis LR., Logan CV., Tan PL., Beer MA., Inglehearn CF., Lewis RA., Jacobson SG., Bergmann C., Beales PL., Attié-Bitach T., Johnson CA., Otto EA., Bhattacharya SS., Hildebrandt F., Gibbs RA., Koenekoop RK., Swaroop A., Katsanis N. swaroopa@nei.nih.gov,katsanis@jhmi.edu Nature genetics 2009 Only protein-protein interactions imex curation imex curation e1bjb4_bovin RPGRIP1L protein protein bovin Bos taurus (Bovine) Bovine MSGPTDETAGDLPVKDIGLNLFGMGGLQETSTTRTMKSRQAVSRISREELEDRFLRLHDENILLKQHARKQEDKIKRMATKLIRLVNDKKRYEQVGGGPKRLGRDVEMEEMIERLQEKVHELERQNEVLKSRLISTKQQLQIQGHRHTPYSYVQSRVNTGRRRVNENAGLQECPRKGIKFQDTDVAETVQTMPTKYSNSLLKEARGEIRNLENVIESQRGQIEELERLAEILKTQLRRKENEIELSLLQLREHQATDQRSNIRDNVELIKLHKQLVEKSNALSVMEGKFIQLQEKQKTLRISHDALMANGDELNLQLKEQRLKCCSLEKQLRSMTFSERRIEELQDRISDLEKERELLKENYDKLYNSAFSAAHEEQWKLKEQQLKIQIAQLETALKSDLTDKTEILDRLKTERDQNEKISQENRQLQLQYLEQKQQLDELKNRMKFYNQESDINADELSEALLLIKAQKSQKNGDLSFLEKVDSKINKDLERSMKELQATHAETVQELEKTRNMLIMQHKINKDYQMEVEAVTQKMENLQQDYELKVEQYVHLLDIRAARIQKLEAQLKDIAYGTKQYKFKPEIMPDDSVDEFDETIHLERGENLFEIHINKVTFSSEVLQASGDKEPVTFCTYAFYDFELQTTPIVRGLHPEYNFTSQYLVHVNDLFLQYIQKNTVTLEVHQAYSTDYETIAACQLRFHEILEKSGRIFCTASLVGTKGDISNFGTVEYWFRLRVPMDQAIRLYRERAKALGYITSNFKGPEQMQWLSHQAPKTAQLSSTDSTDGNLNELHITIRCCNHLQSRASHLQPHPYVVYKFFDFADHDTAIIPSSNDPQFDDHMCFPVPMNMDLDRYLKSESLSFYVFDDSDTQENIYIGKVSVPLISLAHDRCISGIFELTDHEKHPAGTIHVILKWKFAYLPPSGCITTEDLGNFRHKEESEVVQRLLPTSSFSTIIAAPTPKPRRLTTKDKKVSFMDITPHQHYKETSTLPEDRKEISPEVEHIPEREIDMPTVSHIPKVPQESSVDKVKENTEKTQQGKDDDVSLLSEGQLAERSLTSSEDETEITEELEPEDEGDRSASDSDECIIPGPVSKNIKQPPEKIRIEIIALSLNDSRVTADDTIQRLFVECRFYSLPAEETPVSLPKPKSGQWVYYNYSYVIYMDKENNQAKRDILKAILLKQEMPNRSVRFTVVSDPPEDEQDLECEDIGIANVDLADVFREGRDIIEQNIDVLDARASGGGIGKLRVTVEALHALRSVYEQFRDDLEA q9gmd3_bovin RPGR protein protein bovin Bos taurus (Bovine) Bovine KQEIIEKPKQHTTYTESDDSNEYENEEISPKVTEGRVYKQLLAQGMYAMPVAISMETFSDEDVGDDSDQQSPQTSTSAEGLQKGTFRHENKHDVYPLNTEEIEKESDEGQSQKDSEANEIVSEMESDLVKMTDLKDIRKTEENRKNTDTFFDDLPNRYMNIEDEEDKDFVKESKGDKQDMIFDSEQASIEEENSYLEGESESQQSIADGFQQSESIEFNSVEKDDDEVETNQNLWYSRTFIKQRHEEARHRLSRIMAKYEFKCDRLSRIPEEQEEDSEGSGVEEQEIEANEEECGEKEKEETALLSDDLTDRAEVSEGKGKLGGEAEHVPKGGGEGIRKEGNSGVEQRSRTGSEEGEEGEDMGEGVIESLGKGEKDLEEEEQEQKEREQGHQEERSTGRKEAGEREQGGGEGEEEADEEEGEEEKDKEKEEGKEKEEEEEQEGEGEELEEGRWQDGEEGEGKGDEEGQEGGREEEGDREGQEGEEEGEGEEAGEGEEEGEGEEEGEGEEEGEGEEAGEGEEAGEGKGREEEEKGEEGEGEEKVGGEEEEDGEGQEGEEEEEEEGDGEEEEEGDGEEQEAEGGEVEGDGEVEEVGDEEKEGDGEEEGDGEREGGTGEGDEEEQKGEEEEEGDGEGEEEGDGEGEEEGDGEGEEEGDGEEQEGEKEEDGEGEEEGDGEGEEGDGEGEEEGDGEGEEEGDGEGEEEGDGEEQEGEEEEDGEGEEEGDGEQEGEEEEEGGGDEGEEEGEGEEELEEGEGEEEGEAEGEEGEGEEEEGEGEKGGEGLGGEGEEGEEEGGERVEEGEGEREREGMEREEKEWVVRKRKEKENGRGEEKEEDENNEEEKGKCQETGNEESGRQGRKGGGRESKVSKIRGSGKYGKDRTHPKKFTTNREGKGKGHGVQRFKIPVQSKQLLVNGPPGSKKFWNNVLPHYLELK rpgr1_bovin X-linked retinitis pigmentosa GTPase regulator-interacting protein 1 RPGRIP1 protein protein bovin Bos taurus (Bovine) Bovine MIPTSKGKNTKTQPPLSRMTRDELEDSLFRLREEHMLVKELFWKQQDEIKRMRTALLRLTASGRGLRAEAAADESSGSPLNGGGTESGGTAPSSTSVPRCPGSSCSSSAWAPLLPAAPSLASTRDTDSSTPPGHRAEKPKRESRDRLSYTAPPTFKEHVTNEKARGEVASEPSELGHLMTSDTMQVEEPPKSPEKMWSKDENFAQRSSLESTQKATELRASIKENIQLIRLKKLLHERNTSLAVTKAQLTEVQAAYETLLHKNQGILGAAHNALLSQVNELRAELKEESKKAVSLKSQMEDVSILQITLKEFQERVEDLEKERKLLNDNYDKLLESMLDSSNQPQWSHELGEQLQQKVSQLQDQLDVEMKEKREILLQLSQEKAQNKDLELEVTSLLQKHKQEVEDLQNISTFSQSPDRQSAPATHPALFQETIQIQPCEPKNQEEKKLSQMLSELQVSHAETTLELEKTRDMLILQRKINVCYQEELEAMMTKADNENKDHEAKLERLNQLLDLKNKRINQLEEQLKDVAYGTRQLPLCLKPLPAHENEDKVDISPWHQSENLFELHIHQAFLTSAALAQAGDTQPTTFCTYSFYDFETHCTPLVVGPQPLYDFTSQYVVEIDSLFLHYLQGASAQLDLHQAIASEHHTLAAGWICFDRVLETVERVHGSATLTGTGGEVFGVLEYWMRLRFPIRSSLQAYNKRKKAQAYLAANVLGAWEAQKDEPRSGTWKNQNELRVEIIRCCGLRSRSLGAQPSPYVMYRFFTFSDHDTTIIPASSNPYFRDLARFPVLVTSDLDQYLRREALSVYVFDDEDSEPGSYLGRVQVPLLPLAQNKSIQGDFNLTDPVGEPNGSVQVHLDWGSCYLPPENFPKPEAQSEEDTRDGLETSIEEEEASFPPQDQMVSIDTPTEAGQYQAKRKPPQVGERKEREHQVAGYSRRKHGRKTGLQGKNRMEYLSHNLLNGNTLQQVKYIEWKFSGLKISADHVLKNQQKEEEMTSSYSAQILKETPHPVNDKEFCEQASEGSEAQTTDSDEIVTPVSQKCPKADSEKMCIEIVSLAFYPEAEVMCDENVEQVYVEYRFYDLPLSETETPVSLRKPRAGEEIYFHFSKVIDLDPVEQKERRQFLFTMLIGEDPEQGHLKFTVVSDPIEEEKKECQEVGYAYLELWPMLVSGRDILEQDLDIVGPEDQATPIGKLKVSLQAAAALQAIYKEMTEDLCS rpgr_mouse X-linked retinitis pigmentosa GTPase regulator Rpgr protein protein mouse Mus musculus Mouse MAESESLVPDTGAVFTFGKTKFAENIPSKFWFKNDIPICLSCGDEHTAIVTGNNKLYMFGSNNWGQLGLGSKAAIIKPTCIKALKPEKVKLAACGRNHTLVSTDTGGVYAAGGNNEGQLGLGDTDDRDTFHQIVFFTPADTIKQLSAGANTSAALTEDGKLFMWGDNSEGQIGLEDKSNVCIPHEVTVGKPISWISCGYYHSAFVTMDGELYTFGEPENGKLGLPNELLMNHRSPQRVLGIPERVIQVACGGGHTVVLTEKVVYAFGLGQFGQLGLGTFLFETSEPKIIERIKDQKICHISCGENHTALMTELGLLYTFGDGRHGKLGLGMENFTNQFFPTLCSNFLRFAVQLIACGGCHMLVFATPRLGTIDEPKFEDVYEPYISTGSFSINDLSPRSSLNRSLSARLRRRERERPPCSASMVGTLPPLEGTSASTSAYFYPSSPPFHLSVNNYPEKSPSESMEPLDSDYFEDKMNKDTETENSSAVDSENFGETNDILNMTHMMTTSSNEKLLDFSPIQKQQNQDTFEKVMESTPCTENEDSYEYEEMSKIKEVTVYKQYLAKGIYMIRPAEILEAFSDEEVGNGLDQVEEPRVFTDGKGLQSKQVGKESDEEIVSEKKTEVMEVADVKKIRESEENSKSDSLFDDLPDKTMNSESEDNKDIAEERRSSEQNMTFDSETELVEEPDSYMECERHSEQDSAEELEQPKLVEYSSEEKDEKDEKDDDEVETENLWYDRNCTEQETENVFRATRFFPKFDLKHDHLSGIPEEQEGPEDSEGNVVVEQVVQAQKENLEFEGDRKEAKAEAPSDVITEKEAPQLSETVKPEEGEMDEEISILNVEDTVEEERKEGEKEIVEEGSIPETEGSETIDITDEKLDEVLKEEDSASLLQRALREYNENPKGHMYDRVKSSSSEILGGNDPTSKDIKKAKKISFFNRMSLTGQKLMQNTNDPLPEIKPIGDQIALQSDKKDANQNHMGQNLQDSTTPNMEGKSKSCTIL ftm_human Protein fantom RPGRIP1L FTM KIAA1005 NPHP8 Nephrocystin-8 RPGR-interacting protein 1-like protein protein protein human Homo sapiens Human MSGPTDETAGDLPVKDTGLNLFGMGGLQETSTTRTMKSRQAVSRVSREELEDRFLRLHDENILLKQHARKQEDKIKRMATKLIRLVNDKKRYERVGGGPKRLGRDVEMEEMIEQLQEKVHELEKQNETLKNRLISAKQQLQTQGYRQTPYNNVQSRINTGRRKANENAGLQECPRKGIKFQDADVAETPHPMFTKYGNSLLEEARGEIRNLENVIQSQRGQIEELEHLAEILKTQLRRKENEIELSLLQLREQQATDQRSNIRDNVEMIKLHKQLVEKSNALSAMEGKFIQLQEKQRTLRISHDALMANGDELNMQLKEQRLKCCSLEKQLHSMKFSERRIEELQDRINDLEKERELLKENYDKLYDSAFSAAHEEQWKLKEQQLKVQIAQLETALKSDLTDKTEILDRLKTERDQNEKLVQENRELQLQYLEQKQQLDELKKRIKLYNQENDINADELSEALLLIKAQKEQKNGDLSFLVKVDSEINKDLERSMRELQATHAETVQELEKTRNMLIMQHKINKDYQMEVEAVTRKMENLQQDYELKVEQYVHLLDIRAARIHKLEAQLKDIAYGTKQYKFKPEIMPDDSVDEFDETIHLERGENLFEIHINKVTFSSEVLQASGDKEPVTFCTYAFYDFELQTTPVVRGLHPEYNFTSQYLVHVNDLFLQYIQKNTITLEVHQAYSTEYETIAACQLKFHEILEKSGRIFCTASLIGTKGDIPNFGTVEYWFRLRVPMDQAIRLYRERAKALGYITSNFKGPEHMQSLSQQAPKTAQLSSTDSTDGNLNELHITIRCCNHLQSRASHLQPHPYVVYKFFDFADHDTAIIPSSNDPQFDDHMYFPVPMNMDLDRYLKSESLSFYVFDDSDTQENIYIGKVNVPLISLAHDRCISGIFELTDHQKHPAGTIHVILKWKFAYLPPSGSITTEDLGNFIRSEEPEVVQRLPPASSVSTLVLAPRPKPRQRLTPVDKKVSFVDIMPHQSDETSPPPEDRKEISPEVEHIPEIEINMLTVPHVPKVSQEGSVDEVKENTEKMQQGKDDVSLLSEGQLAEQSLASSEDETEITEDLEPEVEEDMSASDSDDCIIPGPISKNIKQSLALSPGLGCSSAISAHCNFRLPGSSDFPASASQVDGITGACHHTQPSEKIRIEIIALSLNDSQVTMDDTIQRLFVECRFYSLPAEETPVSLPKPKSGQWVYYNYSNVIYVDKENNKAKRDILKAILQKQEMPNRSLRFTVVSDPPEDEQDLECEDIGVAHVDLADMFQEGRDLIEQNIDVFDARADGEGIGKLRVTVEALHALQSVYKQYRDDLEA rpgr-rpgrip1l-3 3595552 N/A 3595556 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3595557 unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false Fig. 2c rpgrip1-rpgrip1l-1 3595552 N/A 3595556 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3595558 unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false Fig. 2c rpgr-rpgrip1l-4 3595552 N/A 3595556 unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3595557 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false Fig. 2c rpgr-rpgrip1l-6 3595553 N/A 3595559 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell N/A 3595560 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell rid region sufficient to bind sufficient binding region sufficient to bind range range range range false physical association physical association false false false na rpgr-rpgrip1l-5 3595554 N/A 3595559 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell region xpress tag xpress tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3595560 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q68CZ1:p.Ala229Thr mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false Q68CZ1:p.Thr677Ile mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false Q68CZ1:p.Thr615Pro mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false Q68CZ1:p.Ala695Pro mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 2d, 2e rpgr-rpgrip1l-2 3595555 N/A 3595560 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell rid region sufficient to bind sufficient binding region sufficient to bind range range range range false N/A 3595559 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell exon 1-11 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range false physical association physical association false false false Fig. 2a, 2b