UniProt UniProt http://www.uniprot.org martnez-2004-1 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Martínez C., Sanjuan MA., Dent JA., Karlsson L., Ware J. jware@uams.edu The Biochemical journal 2004 Only protein-protein interactions imex curation imex curation martnez-2004-2 chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 fluorescence imaging fluorescence microscopy fluorescence imaging anti-tag immunocytochemistry anti-tag immunocytochemistry Martínez C., Sanjuan MA., Dent JA., Karlsson L., Ware J. jware@uams.edu The Biochemical journal 2004 Only protein-protein interactions imex curation imex curation martnez-2004-3 chlae-cos Chlorocebus aethiops cos cos anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Martínez C., Sanjuan MA., Dent JA., Karlsson L., Ware J. jware@uams.edu The Biochemical journal 2004 Only protein-protein interactions imex curation imex curation sept4_human Septin-4 SEPTIN4 ARTS Apoptosis-related protein in the TGF-beta signaling pathway Bradeion beta Brain protein H5 CE5B3 beta Cell division control-related protein 2 Cerebral protein 7 PNUTL2 Peanut-like protein 2 SEP4 SEPT4 hucep-7 C17orf47 protein protein human Homo sapiens Human MDRSLGWQGNSVPEDRTEAGIKRFLEDTTDDGELSKFVKDFSGNASCHPPEAKTWASRPQVPEPRPQAPDLYDDDLEFRPPSRPQSSDNQQYFCAPAPLSPSARPRSPWGKLDPYDSSEDDKEYVGFATLPNQVHRKSVKKGFDFTLMVAGESGLGKSTLVNSLFLTDLYRDRKLLGAEERIMQTVEITKHAVDIEEKGVRLRLTIVDTPGFGDAVNNTECWKPVAEYIDQQFEQYFRDESGLNRKNIQDNRVHCCLYFISPFGHGLRPLDVEFMKALHQRVNIVPILAKADTLTPPEVDHKKRKIREEIEHFGIKIYQFPDCDSDEDEDFKLQDQALKESIPFAVIGSNTVVEARGRRVRGRLYPWGIVEVENPGHCDFVKLRTMLVRTHMQDLKDVTRETHYENYRAQCIQSMTRLVVKERNRNKLTRESGTDFPIPAVPPGTDPETEKLIREKDEELRRMQEMLHKIQKQMKENY sept5_human Septin-5 SEPTIN5 Cell division control-related protein 1 PNUTL1 Peanut-like protein 1 SEPT5 protein protein human Homo sapiens Human MSTGLRYKSKLATPEDKQDIDKQYVGFATLPNQVHRKSVKKGFDFTLMVAGESGLGKSTLVHSLFLTDLYKDRKLLSAEERISQTVEILKHTVDIEEKGVKLKLTIVDTPGFGDAVNNTECWKPITDYVDQQFEQYFRDESGLNRKNIQDNRVHCCLYFISPFGHGLRPVDVGFMKALHEKVNIVPLIAKADCLVPSEIRKLKERIREEIDKFGIHVYQFPECDSDEDEDFKQQDRELKESAPFAVIGSNTVVEAKGQRVRGRLYPWGIVEVENQAHCDFVKLRNMLIRTHMHDLKDVTCDVHYENYRAHCIQQMTSKLTQDSRMESPIPILPLPTPDAETEKLIRMKDEELRRMQEMLQRMKQQMQDQ 47054765DEA10D33 sept8_human Septin-8 SEPTIN8 KIAA0202 SEPT8 protein protein human Homo sapiens Human MAATDLERFSNAEPEPRSLSLGGHVGFDSLPDQLVSKSVTQGFSFNILCVGETGIGKSTLMNTLFNTTFETEEASHHEACVRLRPQTYDLQESNVQLKLTIVDAVGFGDQINKDESYRPIVDYIDAQFENYLQEELKIRRSLFDYHDTRIHVCLYFITPTGHSLKSLDLVTMKKLDSKVNIIPIIAKADTISKSELHKFKIKIMGELVSNGVQIYQFPTDDEAVAEINAVMNAHLPFAVVGSTEEVKVGNKLVRARQYPWGVVQVENENHCDFVKLREMLIRVNMEDLREQTHSRHYELYRRCKLEEMGFQDSDGDSQPFSLQETYEAKRKEFLSELQRKEEEMRQMFVNKVKETELELKEKERELHEKFEHLKRVHQEEKRKVEEKRRELEEETNAFNRRKAAVEALQSQALHATSQQPLRKDKDKKNRSDIGAHQPGMSLSSSKVMMTKASVEPLNCSSWWPAIQCCSCLVRDATWREGFL sept4-sept4-1 3533016 N/A 3533019 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 3533019 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Table 2 sept4-sept5-1 3533016 N/A 3533020 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 3533019 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Table 2 sept4-sept8-1 3533016 N/A 3533021 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 3533019 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Table 2 sept5-sept5-1 3533016 N/A 3533020 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 3533020 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed Q99719:p.Ser38Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false Q99719:p.Lys40_Lys41delinsAlaAla mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false Q99719:p.Ser58Asn mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Table 2, 4 sept5-sept8-2 3533016 N/A 3533021 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 3533020 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q99719:p.Ser38Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false Q99719:p.Lys40_Lys41delinsAlaAla mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false Q99719:p.Ser58Asn mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Tables 2, 3, 4 sept8-sept8-1 3533016 N/A 3533021 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 3533021 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association false false false Table 2 sept8-sept5-1 3533016 N/A 3533020 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 3533021 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association false false false Tables 2, 3 sept8-sept5-2 3533017 N/A 3533020 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed c-terminal gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 3533021 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed c-terminal xpress tag xpress tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false colocalization colocalization false false false Fig. 4B sept5-sept8-3 3533018 N/A 3533020 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed c-terminal gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Q99719:p.Ser58Asn mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false N/A 3533021 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed c-terminal xpress tag xpress tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Fig. 5