IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ djabali-2004-1 mouse-nih_3t3 Mus musculus Swiss NIH 3t3 embryo fibroblast cells nih_3t3 Mouse Swiss NIH embryo contact inhibited fibroblast cells confocal microscopy confocal microscopy immunostaining immunostaining Djabali K., Zlotogorski A., Metzker A., Ben-Amitai D., Christiano AM., Djabali K., Zlotogorski A., Metzker A., Ben-Amitai D., Christiano AM. Rare diseases - Interactions investigated in the context of Rare genetic disease TRb1 construct was found to be of mouse origin (pmid: 25774556) amc65@columbia.edu Experimental dermatology 2004 Only protein-protein interactions imex curation imex curation djabali-2004-2 mouse-nih_3t3 Mus musculus Swiss NIH 3t3 embryo fibroblast cells nih_3t3 Mouse Swiss NIH embryo contact inhibited fibroblast cells anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Djabali K., Zlotogorski A., Metzker A., Ben-Amitai D., Christiano AM., Djabali K., Zlotogorski A., Metzker A., Ben-Amitai D., Christiano AM. Rare diseases - Interactions investigated in the context of Rare genetic disease amc65@columbia.edu Experimental dermatology 2004 Only protein-protein interactions imex curation imex curation hair_mouse Lysine-specific demethylase hairless Hr [histone H3]-dimethyl-L-lysine(9) demethylase hairless protein protein mouse Mus musculus Mouse MESMPSFLKDTPAWEKTAPVNGIVGQEPGTSPQDGLRHGALCLGEPAPFWRGVLSTPDSWLPPGFLQGPKDTLSLVEGEGPRNGERKGSWLGGKEGLRWKEAMLAHPLAFCGPACPPRYGPLIPEHSGGHPKSDPVAFRPLHCPFLLETKILERAPFWVPTCLPPYLMSSLPPERPYDWPLAPNPWVYSGSQPKVPSAFGLGSKGFYHKDPNILRPAKEPLAESGMLGLAPGGHLQQACESEGPSLHQRDGETGAGRQQNLCPVFLGYPDTVPRAPWPSCPPGLVHSLGNIWAGPGSNSLGYQLGPPATPRCPSPGPPTPPGGCCSSHLPAREGDLGPCRKCQDSPEGGSSGPGESSEERNKADSRACPPSHHTKLKKTWLTRHSEQFECPGGCSGKEESPATGLRALKRAGSPEVQGASRGPAPKRPSHPFPGTGRQGARAWQETPETIIGSKAEAEQQEEQRGPRDGRIRLQESRLVDTSCQHHLAGVTQCQSCVQAAGEVGVLTGHSQKSRRSPLEEKQLEEEDSSATSEEGGGGPGPEASLNKGLAKHLLSGLGDRLCRLLRKEREALAWAQREGQGPAMTEDSPGIPHCCSRCHHGLFNTHWRCSHCSHRLCVACGRIAGAGKNREKTGSQEQHTDDCAQEAGHAACSLILTQFVSSQALAELSTVMHQVWAKFDIRGHCFCQVDARVWAPGDGGQQKEPTEKTPPTPQPSCNGDSNRTKDIKEETPDSTESPAEDGAGRSPLPCPSLCELLASTAVKLCLGHDRIHMAFAPVTPALPSDDRITNILDSIIAQVVERKIQEKALGPGLRAGSGLRKGLSLPLSPVRTRLSPPGALLWLQEPRPKHGFHLFQEHWRQGQPVLVSGIQKTLRLSLWGMEALGTLGGQVQTLTALGPPQPTNLDSTAFWEGFSHPETRPKLDEGSVLLLHRTLGDKDASRVQNLASSLPLPEYCAHQGKLNLASYLPLGLTLHPLEPQLWAAYGVNSHRGHLGTKNLCVEVSDLISILVHAEAQLPPWYRAQKDFLSGLDGEGLWSPGSQTSTVWHVFRAQDAQRIRRFLQMVCPAGAGTLEPGAPGSCYLDAGLRRRLREEWGVSCWTLLQAPGEAVLVPAGAPHQVQGLVSTISVTQHFLSPETSALSAQLYHQGASLPPDHRMLYAQMDRAVFQAVKAAVGALQEAK p37242-1 Thyroid hormone receptor beta Thrb Erba2 Nr1a2 Nuclear receptor subfamily 1 group A member 2 c-erbA-2 c-erbA-beta protein protein mouse Mus musculus Mouse MTPNSMTENGLPAWDKQKPRPDRGQDWKLVGMSEACLHRKSHVERRGALKNEQTSPHLIQATWTSSIFHLDPDDVNDQSISSAQTFQTEEKKCKGYIPSYLDKDELCVVCGDKATGYHYRCITCEGCKGFFRRTIQKSLHPSYSCKYEGKCIIDKVTRNQCQECRFKKCIYVGMATDLVLDDSKRLAKRKLIEENREKRRREELQKSIGHKPEPTDEEWELIKTVTEAHVATNAQGSHWKQKRKFLPEDIGQAPIVNAPEGGKVDLEAFSHFTKIITPAITRVVDFAKKLPMFCELPCEDQIILLKGCCMEIMSLRAAVRYDPDSETLTLNGEMAVTRGQLKNGGLGVVSDAIFDLGMSLSSFNLDDTEVALLQAVLLMSSDRPGLACVERIEKYQDSFLLAFEHYINYRKHHVTHFWPKLLMKVTDLRMIGACHASRFLHMKVECPTELFPPLFLEVFED hr-thrb-1 3590700 N/A 3590702 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q61645:p.Asp1005Asn mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false N/A 3590703 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false Figure 3a D1005N mutation in hairless gene (UniProt:Q61645) is mouse equivalent of human D1012N hr-thrb-2 3590701 N/A 3590702 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q61645:p.Asp1005Asn mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false N/A 3590703 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Figure 3b