DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ ho-2018-2 plaf7 Plasmodium falciparum (isolate 3D7) 3d-em 3D electron microscopy 3D-EM electron tomog experimental particp experimental participant identification experimental particp Ho CM., Beck JR., Lai M., Cui Y., Goldberg DE., Egea PF., Zhou ZH. pegea@mednet.ucla.edu; hong.zhou@UCLA.edu Nature 2018 imex curation Only protein-protein interactions imex curation ho-2018-4 plaf7 Plasmodium falciparum (isolate 3D7) anti tag coip anti tag coimmunoprecipitation anti tag coip experimental particp experimental participant identification experimental particp Ho CM., Beck JR., Lai M., Cui Y., Goldberg DE., Egea PF., Zhou ZH. pegea@mednet.ucla.edu; hong.zhou@UCLA.edu Nature 2018 imex curation Only protein-protein interactions imex curation q8ila1_plaf7 PF3D7_1436300 protein protein plaf7 Plasmodium falciparum (isolate 3D7) MRIIILALLIVCTIINYYCAVQNNGNKSLNVMPTCSMPGNDSDSNDNETGDVDNDKNNELGNANDNNEMNNENAESKNMQGENSNNQEQLNENVHANDDAMYEGTPSSDNPPQENVDANNNEQEYGPPQEEPVSENNVENVEVATDDSGNDNINNNDNFNNNDNYNDNDNFNEEPPSDDGNKNEDELTEGNQSDDKPMNEEEATINEMGKITNPFEDMLKGKVDDMDIGKMMNKDNLQSFLSSLTGNKDGSGKNPLSDMMNIFGVPQTGKEGAEGGVNKENQMKQINELKDKLETMLKGAGVNVDKIKDSIKNNDLLKNKQLLKEAISKLTLDPSMMNMLNNKDGANGKPFDINPDSMMKMFNALSNENGNLDDLKMKPTDGSFDSFNDGVDNNLVPSNPKGQNNNEEDDEEGGDDDDYDDKSFVVNSKYADNSFEDKFNTFDEKDDDVKYELFGENEEAEELNNNTTTASSKGDANNSVNTQEGEGEEESFSANEENINNNNNHNNKNYNNYNTSQQEEDDNSFNENDEPLISSSQFDNNKKNKMSVSTHNKKSKNLMDSLDLESTNYGSNSSSSMSNNYNSKNKNSKKNNKKKSSQKDYIRTDGKVSFDMATLQKTIKNFGGADNEIVQNILKKYVTIDNDDDNDADEDEDEDDDDDDDLDEDEFSVKDIKKLIEEGILDYEDLTENELRKLAKPDDNFYELSPYASDEKDLSLNETSGLTNEQLKNFLGQNGTYHMSYDSKSIDYAKQKKSEKKEDQQEDDDGFYDAYKQIKNSYDGIPNNFNHEAPQLIGNNYVFTSIYDTKENLIKFLKKNSEYDLYDDDDKEGGNFKSPLYDKYGGKLQKFKRQRAFNILKQWRAKEKKLKEKKKKEEMEENKEFDFSKNYNFSSKNDGGVTMFSKDQLEDMVKNFGGKPSAHVTDSFSRKENPFVPTNTKNNSNDDDDMDNGYVTFDGKNKVSENDDDEKGNNNDDENDNDDSNDEEELDEEEDDN q8ikc8_plaf7 PF3D7_1471100 protein protein plaf7 Plasmodium falciparum (isolate 3D7) MKVSYIFSFFLLFFVYKNTNTVVCDNGYGDLAATSALTTVIKDPISLTIKDIYEHGVKNPFTKIIHKLKKFIRYRKVLRWSRMWWVLLVREIVGDNTIEKKTEKALREIWDQCTIAVYNNTLNAVESKPLLFLHGILNECRNNFATKLRQDPSLIVAKIDQIIKSQIYRFWVSEPYLKIGRSHTLYTHITPDAVPQLPKECTLKHLSSYMEEKLKSMESKKNIESGKYEFDVDSSETDSTKDDGKPDDDDDDDDNFDDDDNFDDDTVEEEDASGDLFKNEKKDENKE q8iij8_plaf7 PF3D7_1116800 protein protein plaf7 Plasmodium falciparum (isolate 3D7) MTRRYLKYYIFVTLLFFVQVINNVLCAPDNKQEQGKYLNRTINILNAGKNIAKSYGHNKLKPIHILSALAKSDYGSTLFKENNVNAANLKEYIDIALEQTRAGAPLDNKSKIVNSAEVKETLALAEAAANKYKSPKVDVEHLLSGLSNDELVNEIFNEVYLTDEAIKAILKRKFEKTKKDKDGKTGTLYIEQFGSNMNEKVRNGKLQGIYGRDEEIRAIIESLLRYNKNSPVLVGNPGTGKTTIVEGLVYRIEKGDVPKELQGYTVISLNFRKFTSGTSYRGEFETRMKNIIKELKNKKNKIILFVDEIHLLLGAGKAEGGTDAANLLKPVLSKGEIKLIGATTIAEYRKFIESCSAFERRFEKILVEPPSVDMTVKILRSLKSKYENFYGINITDKALVAAAKISDRFIKDRYLPDKAIDLLNKACSFLQVQLSGKPRIIDVTERDIERLSYEISTLEKDVDKVSKKKYNKLIKEFEEKKEQLKKYYEEYVITGERLKRKKEIEKKLNDLKELTQNYVYSNKEPPIELQNSLKEAQQKYLELYKETVAYVEAKTHNAMNVDAVYQEHVSYIYLRDSGMPLGSLSFESSKGALKLYNSLSKSIIGNEDIIKSLSDAVVKAATGMKDPEKPIGTFLFLGPTGVGKTELAKTLAIELFNSKDNLIRVNMSEFTEAHSVSKITGSPPGYVGFSDSGQLTEAVREKPHSVVLFDELEKAHADVFKVLLQILGDGYINDNHRRNIDFSNTIIIMTSNLGAELFKKKLFFDADNSGTPEYKRVMEDVRLSLIKKCKKVFKPEFVNRIDKIGVFEPLNKKNLHKIVALRFKKLEKRLEEKNIQVSVSEKAIDYIIDQSYDPELGARPTLIFIESVIMTKFAIMYLKKELVDDMDVFVDYNSKAKNLVINLSKT q8ijx8_plaf7 PF3D7_1006200 protein protein plaf7 Plasmodium falciparum (isolate 3D7) MASTEEVSQERSENSIQVSMTKKPTFYARIGKRMFTGNEEKNPFDEVIITGLGNATKIAIGAASIMEKEDIGQIVKVQTAYFSSDRINRRIPKITIVLKKHPDFVAN q8i2x4_plaf7 PF3D7_0917900 protein protein plaf7 Plasmodium falciparum (isolate 3D7) MKQIRPYILLLIVSLLKFISAVDSNIEGPVIGIDLGTTYSCVGVFKNGRVEILNNELGNRITPSYVSFVDGERKVGEAAKLEATLHPTQTVFDVKRLIGRKFDDQEVVKDRSLLPYEIVNNQGKPNIKVQIKDKDTTFAPEQISAMVLEKMKEIAQSFLGKPVKNAVVTVPAYFNDAQRQATKDAGTIAGLNIVRIINEPTAAALAYGLDKKEETSILVYDLGGGTFDVSILVIDNGVFEVYATAGNTHLGGEDFDQRVMDYFIKMFKKKNNIDLRTDKRAIQKLRKEVEIAKRNLSVVHSTQIEIEDIVEGHNFSETLTRAKFEELNDDLFRETLEPVKKVLDDAKYEKSKIDEIVLVGGSTRIPKIQQIIKEFFNGKEPNRGINPDEAVAYGAAIQAGIILGEELQDVVLLDVTPLTLGIETVGGIMTQLIKRNTVIPTKKSQTFSTYQDNQPAVLIQVFEGERALTKDNHLLGKFELSGIPPAQRGVPKIEVTFTVDKNGILHVEAEDKGTGKSRGITITNDKGRLSKEQIEKMINDAEKFADEDKNLREKVEAKNNLDNYIQSMKATVEDKDKLADKIEKEDKNTILSAVKDAEDWLNNNSNADSEALKQKLKDLEAVCQPIIVKLYGQPGGPSPQPSGDEDVDSDEL q8iij8_plaf7-q8ikc8_plaf7-1 3474568 N/A 3474570 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified plaf7 Plasmodium falciparum (isolate 3D7) Stoichiometry: 7.0 N/A 3474571 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified plaf7 Plasmodium falciparum (isolate 3D7) Stoichiometry: 7.0 N/A 3474572 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified Flag Tag Flag Tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false plaf7 Plasmodium falciparum (isolate 3D7) Stoichiometry: 6.0 physical association physical association false false false Fig. 1, Extended Data Table 1 q8iij8_plaf7-q8i2x4_plaf7-1 3474569 N/A 3474573 unspecified role unspecified role prey prey native native plaf7 Plasmodium falciparum (isolate 3D7) N/A 3474570 mudpit multidimensional protein identification technology mudpit unspecified role unspecified role prey prey native native plaf7 Plasmodium falciparum (isolate 3D7) N/A 3474571 mudpit multidimensional protein identification technology mudpit unspecified role unspecified role prey prey native native plaf7 Plasmodium falciparum (isolate 3D7) N/A 3474572 predetermined predetermined participant predetermined mudpit multidimensional protein identification technology mudpit unspecified role unspecified role bait bait native native Flag Tag Flag Tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false plaf7 Plasmodium falciparum (isolate 3D7) N/A 3474574 mudpit multidimensional protein identification technology mudpit unspecified role unspecified role prey prey native native plaf7 Plasmodium falciparum (isolate 3D7) association association false false false Extended Data Fig. 1e, f