DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ gruszczyk-2018-6 in vitro In vitro 3d-em 3D electron microscopy 3D-EM electron tomog predetermined predetermined participant predetermined Gruszczyk J., Huang RK., Chan LJ., Menant S., Hong C., Murphy JM., Mok YF., Griffin MDW., Pearson RD., Wong W., Cowman AF., Yu Z., Tham WH., Gruszczyk J., Huang RK., Chan LJ., Menant S., Hong C., Murphy JM., Mok YF., Griffin MDW., Pearson RD., Wong W., Cowman AF., Yu Z., Tham WH. yuz@janelia.hhmi.org Nature 2018 imex curation Only protein-protein interactions imex curation gruszczyk-2018-8 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Gruszczyk J., Huang RK., Chan LJ., Menant S., Hong C., Murphy JM., Mok YF., Griffin MDW., Pearson RD., Wong W., Cowman AF., Yu Z., Tham WH., Gruszczyk J., Huang RK., Chan LJ., Menant S., Hong C., Murphy JM., Mok YF., Griffin MDW., Pearson RD., Wong W., Cowman AF., Yu Z., Tham WH. yuz@janelia.hhmi.org Nature 2018 imex curation Only protein-protein interactions imex curation gruszczyk-2018-7 in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Gruszczyk J., Huang RK., Chan LJ., Menant S., Hong C., Murphy JM., Mok YF., Griffin MDW., Pearson RD., Wong W., Cowman AF., Yu Z., Tham WH., Gruszczyk J., Huang RK., Chan LJ., Menant S., Hong C., Murphy JM., Mok YF., Griffin MDW., Pearson RD., Wong W., Cowman AF., Yu Z., Tham WH. yuz@janelia.hhmi.org Nature 2018 imex curation Only protein-protein interactions imex curation a5k736_plavs PVX_094255 protein protein Plasmodium vivax (strain Salvador I) Plasmodium vivax (strain Salvador I) MHIPIQPSPESTQITNTTDNIDYFDISDESNYYLISQLRPHFSNIYFFDEFKRYASYHTEIKRYEDIHKTKVNSLLNEASRAIGICNRAKNTVKGLINILENPQKFKTQRESYDVKLRQYEEKKEAFRGCLLNKNRKNLDQIKKINNEIRDLLEKLKCSQDCQTNVYFDMIKIYLVDFKKMPYENYDTFIKQYKNSYLSGVDMIRKIEKQIDNPVTINAIKFTQKEMGYIIDRFEYHLQKVKHSIDQVTALSDGVKPKQVTKNRLKEYYFNIGNYYSIFKFGKDSLNMLNKALIHKEKIVHNLLGELFGHLEERISKLIDSEYFITESNNIISQSEETLKLAEDVYDKNTKLIEDLTLYPHLEINEFKKDYDNNVEDLRESIIYIQSYVSSIKSAYRYNVLEKDSVESKQKNIPANSNAQKKVDELLSIIDSISYSNFSVAENFQKMKDYYKEIEKLKIKILQLIEAIKKYQQHVEELINKEKAVAILKEDINKIIEYIKGIIEKLKQLISANKDFDKIFQQVEQLINEALFNKDQFEHNKNDLHTKMKEIMHTFHERDLQQFLDNMSKFLKDQEASYQNADSKEKLDQLLTTVKAKQDELKEMKCDDIPDIIDNLKKESQNVLNLKDEVINKQFENMRTEMSSSLDQMTKEYNALKSSIEEYEAEKKGIENHKQNIIKRKNTFIVAEHENDEDVPEGKNTYNEFISNKDTILQKESAISNQMNTLEEKKRNRKTTLQTYGDAIQKLETYTEKKDEETKVLLDKFNTEVENFKLDEDEKSFNDAKSIVSNTINEVENENKNIDSIKKVNIAMKRSRSNQQSVEQLIRNKDNLLKEKMEKHFQKINTDEIIGQSAKANLLNNLESEKAIINEQLNDAHLNDLKLQIEKTLSYCEKSRENIKGNNGTHPEELNASEIEWEATKGKIETLNSNYQALNKNMDDIINKQHDEMIVLIDRDIKEKGKDIELKVEQRIQALEKMKAKLSSFDTNEDVKKYQSDINKEELIKLKEMSTAVLNKIDQNKNQLINVKTKSSGYVAESNGIKNKQTEPSQEKDKQIVYNTNRGDIEKVYKNIRDMYNELDDVEKEETTSNEVDNIEMQYDRLLINLLVQKINEENTKAKGLMEEIKSYIENIESVQAKMSDETREGATEFEFNQYQQSSLANDKKITEIVQHAINKKGEAERSKKTNEVKSIKNDVNTYMQQVIDEYNAMQKALGDIKNVNDLLISTSGRRIAEEISKSTNEAENFEKQAKGELGKINDIIEKMENQMVQAKEHENKIHINLEDKQIDEEVQKIRQIHEELINTKIAMNEHLSKTKAAKENCLSQVRNAERGKIKIDFLNNNKQSSNLKDVNMDHVTKNIADCVEHSKEVNKTENEAEKEHDLFLEYEKDITSILNRSSILGIETKSKKRKNEATKIMGQIEGEHSIIKTELQQSSENFKQLNEKYNLSEEKSMLANPTSTKANESIQYNIGKVKQNLLYIDNIAKEVDTILTNAQDSIRSISQISKLDGRNTLQMVEKEAADYVKYLDKITKEKELMNDKHKDLNPIKSDIANIETELKKHRKLFEIGLLNKVIEIAKTRKLYMDSVEQLLNSSINNFVTLFNGFNLKKYKIETSLDVYKGKISEFHTEFLQPYESIQNKEKRVLDPSVDFSAAKNMREEAQKEEKNIENVEKKTKKYLSDINKMESFTFIRNMRQTLDELSKTCKQEYAEVEGGHKDIEKIIEDIKSLNDESGSSAKLQQAEDKNSQMKAKVNHYSLKKEAHTILEQIVKSANFVGITIVTALQPSDLAAKASLQKAPELQFESQSKVTLESGQFLEKKNQLDVHENVQAAYNTVLQIYKYSDDIVRKQEKCEQLVKDGKDICLKFKSINEIKVMIQNSKGKESTLSAKVSHSFNKLSELNKIKCNDESYDAILETPSREELNKLRSTFKQEKDTIANQAKLSGYKTDFETHIGKLNDLAKIVDNLKASETLPKNIEEKKTSINLISTKLETIEKEIESINSSFDQLLEKGKKCEMTKYKLVRDSLSTKINDHSAIIKDNQKKATEYLTYIQNNHISIFKDIDMLNENLGEKSVSRYAIAKIEEANDLSAQLTAAVSEYEAIANSIRKEFTNISDHTEMDTLENEAKMLKEHYDNLINKKNIITELHNKINLIKLLEIRATSDKYVDIAELLGEVVKDQKKKLQEAKNKLDTLKDHIAVKEKELINHDSSFTLVSIKAFDEIYDDIKYNVGQLHTLEVTNFDELKKGKTYEENVTHLLNRRETLQNDLHNYEEKDKLKNTNIEMSNEENNQIRQTSEVIKKLESEFQNLLKIIQQSNTLCSNDNIKQFISDILKKVETIRERFVKNFPEREKYHQIEINYNEIKGIVKEVDTNPEISIFTEKINTYIRQKIRSAHHLEDAQKIKDIIEDVTSNYRKIKSKLSQVNNALDRIKIKKSEMDTLFESLSKENANNYNSAKYFLVDSDKIIKHLEDQVSKMSSLISYAEREIKELEEKVYSILNRPVIDNSASEQIKLLSDTQENDTDEAQKREFAEEQILNLPKQQIHSDEGNNNDMSTTDSANEEKQTGEEESQHEDQSQPRGIHSRVRLAEGIILGLSVFSGIIFISFRHKDEEKKDPESVGEQFEGNNNFNEDDKEEVIDVCFDNSDYSE trfe_human Serotransferrin Siderophilin Beta-1 metal-binding globulin TF PRO1400 protein protein human Homo sapiens Human MRLAVGALLVCAVLGLCLAVPDKTVRWCAVSEHEATKCQSFRDHMKSVIPSDGPSVACVKKASYLDCIRAIAANEADAVTLDAGLVYDAYLAPNNLKPVVAEFYGSKEDPQTFYYAVAVVKKDSGFQMNQLRGKKSCHTGLGRSAGWNIPIGLLYCDLPEPRKPLEKAVANFFSGSCAPCADGTDFPQLCQLCPGCGCSTLNQYFGYSGAFKCLKDGAGDVAFVKHSTIFENLANKADRDQYELLCLDNTRKPVDEYKDCHLAQVPSHTVVARSMGGKEDLIWELLNQAQEHFGKDKSKEFQLFSSPHGKDLLFKDSAHGFLKVPPRMDAKMYLGYEYVTAIRNLREGTCPEAPTDECKPVKWCALSHHERLKCDEWSVNSVGKIECVSAETTEDCIAKIMNGEADAMSLDGGFVYIAGKCGLVPVLAENYNKSDNCEDTPEAGYFAIAVVKKSASDLTWDNLKGKKSCHTAVGRTAGWNIPMGLLYNKINHCRFDEFFSEGCAPGSKKDSSLCKLCMGSGLNLCEPNNKEGYYGYTGAFRCLVEKGDVAFVKHQTVPQNTGGKNPDPWAKNLNEKDYELLCLDGTRKPVEEYANCHLARAPNHAVVTRKDKEACVHKILRQQQHLFGSNVTDCSGNFCLFRSETKDLLFRDDTVCLAKLHDRNTYEKYLGEEYVKAVGNLRKCSTSSLLEACTFRRP tfr1_human Transferrin receptor protein 1 TFRC T9 p90 protein protein human Homo sapiens Human MMDQARSAFSNLFGGEPLSYTRFSLARQVDGDNSHVEMKLAVDEEENADNNTKANVTKPKRCSGSICYGTIAVIVFFLIGFMIGYLGYCKGVEPKTECERLAGTESPVREEPGEDFPAARRLYWDDLKRKLSEKLDSTDFTGTIKLLNENSYVPREAGSQKDENLALYVENQFREFKLSKVWRDQHFVKIQVKDSAQNSVIIVDKNGRLVYLVENPGGYVAYSKAATVTGKLVHANFGTKKDFEDLYTPVNGSIVIVRAGKITFAEKVANAESLNAIGVLIYMDQTKFPIVNAELSFFGHAHLGTGDPYTPGFPSFNHTQFPPSRSSGLPNIPVQTISRAAAEKLFGNMEGDCPSDWKTDSTCRMVTSESKNVKLTVSNVLKEIKILNIFGVIKGFVEPDHYVVVGAQRDAWGPGAAKSGVGTALLLKLAQMFSDMVLKDGFQPSRSIIFASWSAGDFGSVGATEWLEGYLSSLHLKAFTYINLDKAVLGTSNFKVSASPLLYTLIEKTMQNVKHPVTGQFLYQDSNWASKVEKLTLDNAAFPFLAYSGIPAVSFCFCEDTDYPYLGTTMDTYKELIERIPELNKVARAAAEVAGQFVIKLTHDVELNLDYERYNSQLLSFVRDLNQYRADIKEMGLSLQWLYSARGDFFRATSRLTTDFGNAEKTDRFVMKKLNDRVMRVEYHFLSPYVSPKESPFRHVFWGSGSHTLPALLENLKLRKQNNGAFNETLFRNQLALATWTIQGAANALSGDVWDIDNEF a5k736_plavs-tf-5 3451304 N/A 3451307 weight by comassie molecular weight estimation by coomasie staining weight by comassie structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3451308 weight by comassie molecular weight estimation by coomasie staining weight by comassie structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified human Homo sapiens Human Stoichiometry: 2.0 N/A 3451309 weight by comassie molecular weight estimation by coomasie staining weight by comassie structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. Stoichiometry: 2.0 physical association physical association false false false Fig 1, Extended Data Fig 1, Extended Data Fig 6f a5k736_plavs-tf-6 3451304 N/A 3451307 weight by comassie molecular weight estimation by coomasie staining weight by comassie structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 N/A 3451308 weight by comassie molecular weight estimation by coomasie staining weight by comassie structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified human Homo sapiens Human Stoichiometry: 2.0 N/A 3451309 weight by comassie molecular weight estimation by coomasie staining weight by comassie structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. Stoichiometry: 2.0 physical association physical association false false false Fig 1, Extended Data Fig 1, Extended Data Fig 6f a5k736_plavs-tf-8 3451305 N/A 3451307 unspecified role unspecified role neutral component neutral component purified purified A5K736:p.Arg150Ala p.Arg150Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false A5K736:p.Tyr450Phe p.Tyr450Phe mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false A5K736:p.Lys409Ala p.Lys409Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false A5K736:p.Glu376Ala p.Glu376Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false A5K736:p.Tyr384Phe p.Tyr384Phe mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false A5K736:p.Tyr388Ala p.Tyr388Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false A5K736:p.Tyr388Phe p.Tyr388Phe mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false A5K736:p.Lys446Ala p.Lys446Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false A5K736:p.Lys446Glu p.Lys446Glu mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false A5K736:p.Lys446Met p.Lys446Met mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false A5K736:p.Tyr388Ala p.Tyr388Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false A5K736:p.Tyr450Ala p.Tyr450Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false A5K736:p.Tyr32Ala p.Tyr32Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false A5K736:p.Asn274Ala p.Asn274Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false A5K736:p.Lys143Ala p.Lys143Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false A5K736:p.Asp377Ala p.Asp377Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false A5K736:p.Glu402Ala p.Glu402Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false A5K736:p.Tyr384Ala p.Tyr384Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 N/A 3451308 unspecified role unspecified role neutral component neutral component purified purified human Homo sapiens Human Stoichiometry: 2.0 N/A 3451309 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. Stoichiometry: 2.0 physical association physical association false false false Fig 3b, 3c & 3e; Extended data Fig 4c a5k736_plavs-tf-3 3451305 N/A 3451307 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 N/A 3451308 unspecified role unspecified role neutral component neutral component purified purified human Homo sapiens Human Stoichiometry: 2.0 N/A 3451309 unspecified role unspecified role neutral component neutral component purified purified P02786:p.Tyr211Ala p.Tyr211Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P02786:p.Glu294Ala p.Glu294Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P02786:p.Glu149Ala p.Glu149Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P02786:p.Asn348Ala p.Asn348Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P02786:p.Glu578Ala p.Glu578Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P02786:p.Arg208Ala p.Arg208Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P02786:p.Lys574Ala p.Lys574Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P02786:p.Glu214Ala p.Glu214Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. Stoichiometry: 2.0 physical association physical association false false false Fig 3d a5k736_plavs-tf-7 3451306 N/A 3451307 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 N/A 3451308 unspecified role unspecified role neutral component neutral component purified purified human Homo sapiens Human Stoichiometry: 2.0 N/A 3451309 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. Stoichiometry: 2.0 physical association physical association false false false Extended Data Fig 2