DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ weidberg-2018-1 yeast Saccharomyces cerevisiae Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Weidberg H., Amon A. angelika@mit.edu Science (New York, N.Y.) 2018 imex curation Only protein-protein interactions imex curation msp1_yeast Outer mitochondrial transmembrane helix translocase MSP1 YTA4 Tat-binding homolog 4 YGR028W Mitochondrial sorting of proteins protein protein yeast Saccharomyces cerevisiae Baker's yeast MSRKFDLKTITDLSVLVGTGISLYYLVSRLLNDVESGPLSGKSRESKAKQSLQWEKLVKRSPALAEVTLDAYERTILSSIVTPDEINITFQDIGGLDPLISDLHESVIYPLMMPEVYSNSPLLQAPSGVLLYGPPGCGKTMLAKALAKESGANFISIRMSSIMDKWYGESNKIVDAMFSLANKLQPCIIFIDEIDSFLRERSSTDHEVTATLKAEFMTLWDGLLNNGRVMIIGATNRINDIDDAFLRRLPKRFLVSLPGSDQRYKILSVLLKDTKLDEDEFDLQLIADNTKGFSGSDLKELCREAALDAAKEYIKQKRQLIDSGTIDVNDTSSLKIRPLKTKDFTKKLRMDATSTLSSQPLD atg31_yeast Autophagy-related protein 31 ATG31 CIK1 suppressor protein 1 CIS1 Protein CIS1 PZD196 YD9335.08c YDR022C protein protein yeast Saccharomyces cerevisiae Baker's yeast MNVTVTVYDKNVKYRLEENIKNNKGPSNDDQPAYNNESKSTDGSDYAMFPTNIKYIFEDNNDELVDSSDAALTAGIDKVGDELENVIIVQLDESGSLEDITLISDQYELLSHRTNSLSLEENQMRTLSSHGDDKSNDEEEELSVDSDRFRVDSDIELDVISQFCDLSPFLRDLSLNDLIKLYVTQNEQLQMLSNSV CC2396854F29D250 tom70_yeast Mitochondrial import receptor subunit TOM70 TOM70 MAS70 YNL121C N1905 OMP1 70 kDa mitochondrial outer membrane protein Translocase of outer membrane 70 kDa subunit protein protein yeast Saccharomyces cerevisiae Baker's yeast MKSFITRNKTAILATVAATGTAIGAYYYYNQLQQQQQRGKKNTINKDEKKDTKDSQKETEGAKKSTAPSNPPIYPVSSNGEPDFSNKANFTAEEKDKYALALKDKGNQFFRNKKYDDAIKYYNWALELKEDPVFYSNLSACYVSVGDLKKVVEMSTKALELKPDYSKVLLRRASANEGLGKFADAMFDLSVLSLNGDFNDASIEPMLERNLNKQAMSKLKEKFGDIDTATATPTELSTQPAKERKDKQENLPSVTSMASFFGIFKPELTFANYDESNEADKELMNGLSNLYKRSPESYDKADESFTKAARLFEEQLDKNNEDEKLKEKLAISLEHTGIFKFLKNDPLGAHEDIKKAIELFPRVNSYIYMALIMADRNDSTEYYNYFDKALKLDSNNSSVYYHRGQMNFILQNYDQAGKDFDKAKELDPENIFPYIQLACLAYRENKFDDCETLFSEAKRKFPEAPEVPNFFAEILTDKNDFDKALKQYDLAIELENKLDGIYVGIAPLVGKATLLTRNPTVENFIEATNLLEKASKLDPRSEQAKIGLAQMKLQQEDIDEAITLFEESADLARTMEEKLQAITFAEAAKVQQRIRSDPVLAKKIQETLAKLREQGLM 664B8FC32CCE39A2 atg31-msp1-1 3450284 N/A 3450285 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed P28737:p.Glu193Gln E193Q mutation mutation certain certain sequence position certain certain certain sequence position certain false Flag tag Flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 3450286 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed V5 tag V5 tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Fig 7A msp1-atg31-1 3450284 N/A 3450285 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed Flag tag Flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false P28737:p.Glu193Gln E193Q mutation mutation certain certain sequence position certain certain certain sequence position certain false N/A 3450286 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed V5 tag V5 tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Fig S6-A & Fig S6-B atg31-msp1-2 3450284 N/A 3450287 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed GFP tag GFP tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 3450285 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed P28737:p.Glu193Gln E193Q mutation mutation certain certain sequence position certain certain certain sequence position certain false Flag tag Flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 3450286 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed V5 tag V5 tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false association association false false false Fig 7C msp1-tom70-1 3450284 N/A 3450287 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed GFP tag GFP tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 3450285 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed P28737:p.Glu193Gln E193Q mutation mutation certain certain sequence position certain certain certain sequence position certain false Flag tag Flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Fig 7D