IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ zhou-2018-6 in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Zhou Y., He CH., Yang DS., Nguyen T., Cao Y., Kamle S., Lee CM., Gochuico BR., Gahl WA., Shea BS., Lee CG., Elias JA. Recombinant proteins are stated to have been used;it has been assumed these were supplied by ACROBiosystems (as stated for SPR), but this supplier does not sell TMEM219. jack_elias@brown.edu, yang_zhou@brown.edu Journal of immunology (Baltimore, Md. : 1950) 2018 Only protein-protein interactions imex curation imex curation zhou-2018-1 mouse-lung Mus musculus lung anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Zhou Y., He CH., Yang DS., Nguyen T., Cao Y., Kamle S., Lee CM., Gochuico BR., Gahl WA., Shea BS., Lee CG., Elias JA. jack_elias@brown.edu, yang_zhou@brown.edu Journal of immunology (Baltimore, Md. : 1950) 2018 Only protein-protein interactions imex curation imex curation zhou-2018-2 human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Zhou Y., He CH., Yang DS., Nguyen T., Cao Y., Kamle S., Lee CM., Gochuico BR., Gahl WA., Shea BS., Lee CG., Elias JA. jack_elias@brown.edu, yang_zhou@brown.edu Journal of immunology (Baltimore, Md. : 1950) 2018 Only protein-protein interactions imex curation imex curation zhou-2018-3 human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Zhou Y., He CH., Yang DS., Nguyen T., Cao Y., Kamle S., Lee CM., Gochuico BR., Gahl WA., Shea BS., Lee CG., Elias JA. jack_elias@brown.edu, yang_zhou@brown.edu Journal of immunology (Baltimore, Md. : 1950) 2018 Only protein-protein interactions imex curation imex curation zhou-2018-4 mouse-lung Mus musculus lung fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Zhou Y., He CH., Yang DS., Nguyen T., Cao Y., Kamle S., Lee CM., Gochuico BR., Gahl WA., Shea BS., Lee CG., Elias JA. IL-13 Tg mice; mice over-expressing IL13. jack_elias@brown.edu, yang_zhou@brown.edu Journal of immunology (Baltimore, Md. : 1950) 2018 Only protein-protein interactions imex curation imex curation zhou-2018-5 mouse-pam mouse pam alveolar_macrophage Alveolar macrophage fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Zhou Y., He CH., Yang DS., Nguyen T., Cao Y., Kamle S., Lee CM., Gochuico BR., Gahl WA., Shea BS., Lee CG., Elias JA. CD68+ macrophages from IL-13 Tg mice; mice over-expressing IL13. jack_elias@brown.edu, yang_zhou@brown.edu Journal of immunology (Baltimore, Md. : 1950) 2018 Only protein-protein interactions imex curation imex curation p36222-pro_0000011965 Chitinase-3-like protein 1 39 kDa synovial protein Cartilage glycoprotein 39 YKL-40 CHI3L1 protein protein human Homo sapiens Human YKLVCYYTSWSQYREGDGSCFPDALDRFLCTHIIYSFANISNDHIDTWEWNDVTLYGMLNTLKNRNPNLKTLLSVGGWNFGSQRFSKIASNTQSRRTFIKSVPPFLRTHGFDGLDLAWLYPGRRDKQHFTTLIKEMKAEFIKEAQPGKKQLLLSAALSAGKVTIDSSYDIAKISQHLDFISIMTYDFHGAWRGTTGHHSPLFRGQEDASPDRFSNTDYAVGYMLRLGAPASKLVMGIPTFGRSFTLASSETGVGAPISGPGIPGRFTKEAGTLAYYEICDFLRGATVHRILGQQVPYATKGNQWVGYDDQESVKSKVQYLKDRQLAGAMVWALDLDDFQGSFCGQDLRFPLTNAIKDALAAT 22 383 leg3_human Galectin-3 LGALS3 MAC2 Galactose-specific lectin 3 Mac-2 antigen IgE-binding protein 35 kDa lectin Carbohydrate-binding protein 35 Laminin-binding protein Lectin L-29 L-31 Galactoside-binding protein protein protein human Homo sapiens Human MADNFSLHDALSGSGNPNPQGWPGAWGNQPAGAGGYPGASYPGAYPGQAPPGAYPGQAPPGAYPGAPGAYPGAPAPGVYPGPPSGPGAYPSSGQPSATGAYPATGPYGAPAGPLIVPYNLPLPGGVVPRMLITILGTVKPNANRIALDFQRGNDVAFHFNPRFNENNRRVIVCNTKLDNNWGREERQSVFPFESGKPFKIQVLVEPDHFKVAVNDAHLLQYNHRVKKLNEISKLGISGDIDLTSASYTMI i13r2_human Interleukin-13 receptor subunit alpha-2 IL13RA2 IL13R Interleukin-13-binding protein protein protein human Homo sapiens Human MAFVCLAIGCLYTFLISTTFGCTSSSDTEIKVNPPQDFEIVDPGYLGYLYLQWQPPLSLDHFKECTVEYELKYRNIGSETWKTIITKNLHYKDGFDLNKGIEAKIHTLLPWQCTNGSEVQSSWAETTYWISPQGIPETKVQDMDCVYYNWQYLLCSWKPGIGVLLDTNYNLFYWYEGLDHALQCVDYIKADGQNIGCRFPYLEASDYKDFYICVNGSSENKPIRSSYFTFQLQNIVKPLPPVYLTFTRESSCEIKLKWSIPLGPIPARCFDYEIEIREDDTTLVTATVENETYTLKTTNETRQLCFVVRSKVNIYCSDDGIWSEWSDKQCWEGEDLSKKTLLRFWLPFGFILILVIFVTGLLLRKPNTYPKMIPEFFCDT tm219_human Insulin-like growth factor-binding protein 3 receptor TMEM219 Transmembrane protein 219 protein protein human Homo sapiens Human MGNCQAGHNLHLCLAHHPPLVCATLILLLLGLSGLGLGSFLLTHRTGLRSPDIPQDWVSFLRSFGQLTLCPRNGTVTGKWRGSHVVGLLTTLNFGDGPDRNKTRTFQATVLGSQMGLKGSSAGQLVLITARVTTERTAGTCLYFSAVPGILPSSQPPISCSEEGAGNATLSPRMGEECVSVWSHEGLVLTKLLTSEELALCGSRLLVLGSFLLLFCGLLCCVTAMCFHPRRESHWSRTRL ch3l1_mouse Chitinase-3-like protein 1 Breast regression protein 39 Chil1 Chi3l1 Brp39 Cartilage glycoprotein 39 BRP39 protein protein protein mouse Mus musculus Mouse MHTSTEARMGMRAALTGFAVLMLLQSCSAYKLVCYFTSWSQYREGVGSFLPDAIQPFLCTHIIYSFANISSDNMLSTWEWNDESNYDKLNKLKTRNTNLKTLLSVGGWKFGEKRFSEIASNTERRTAFVRSVAPFLRSYGFDGLDLAWLYPRLRDKQYFSTLIKELNAEFTKEVQPGREKLLLSAALSAGKVAIDTGYDIAQIAQHLDFINLMTYDFHGVWRQITGHHSPLFQGQKDTRFDRYSNVNYAVQYMIRLGAQASKLLMGIPTFGKSFTLASSENQLGAPISGEGLPGRFTKEAGTLAYYEICDFLKGAEVHRLSNEKVPFATKGNQWVGYEDKESVKNKVGFLKEKKLAGAMVWALDLDDFQGTCQPKEFFPLTNAIKDALA mint May play an important role in the capacity of cells to respond to and cope with changes in their environment. May play a role in tissue remodeling and defense against pathogens i13r2_mouse Interleukin-13 receptor subunit alpha-2 Il13ra2 protein protein mouse Mus musculus Mouse MAFVHIRCLCFILLCTITGYSLEIKVNPPQDFEILDPGLLGYLYLQWKPPVVIEKFKGCTLEYELKYRNVDSDSWKTIITRNLIYKDGFDLNKGIEGKIRTHLSEHCTNGSEVQSPWIEASYGISDEGSLETKIQDMKCIYYNWQYLVCSWKPGKTVYSDTNYTMFFWYEGLDHALQCADYLQHDEKNVGCKLSNLDSSDYKDFFICVNGSSKLEPIRSSYTVFQLQNIVKPLPPEFLHISVENSIDIRMKWSTPGGPIPPRCYTYEIVIREDDISWESATDKNDMKLKRRANESEDLCFFVRCKVNIYCADDGIWSEWSEEECWEGYTGPDSKIIFIVPVCLFFIFLLLLLCLIVEKEEPEPTLSLHVDLNKEVCAYEDTLC leg3_mouse Galectin-3 Lgals3 35 kDa lectin Carbohydrate-binding protein 35 Galactose-specific lectin 3 IgE-binding protein L-34 galactoside-binding lectin Laminin-binding protein Lectin L-29 Mac-2 antigen protein protein mouse Mus musculus Mouse MADSFSLNDALAGSGNPNPQGYPGAWGNQPGAGGYPGAAYPGAYPGQAPPGAYPGQAPPGAYPGQAPPSAYPGPTAPGAYPGPTAPGAYPGQPAPGAFPGQPGAPGAYPQCSGGYPAAGPYGVPAGPLTVPYDLPLPGGVMPRMLITIMGTVKPNANRIVLDFRRGNDVAFHFNPRFNENNRRVIVCNTKQDNNWGKEERQSAFPFESGKPFKIQVLVEADHFKVAVNDAHLLQYNHRMKNLREISQLGISGDITLTSANHAMI ch3l1_human Chitinase-3-like protein 1 CHI3L1 39 kDa synovial protein Cartilage glycoprotein 39 YKL-40 protein protein human Homo sapiens Human MGVKASQTGFVVLVLLQCCSAYKLVCYYTSWSQYREGDGSCFPDALDRFLCTHIIYSFANISNDHIDTWEWNDVTLYGMLNTLKNRNPNLKTLLSVGGWNFGSQRFSKIASNTQSRRTFIKSVPPFLRTHGFDGLDLAWLYPGRRDKQHFTTLIKEMKAEFIKEAQPGKKQLLLSAALSAGKVTIDSSYDIAKISQHLDFISIMTYDFHGAWRGTTGHHSPLFRGQEDASPDRFSNTDYAVGYMLRLGAPASKLVMGIPTFGRSFTLASSETGVGAPISGPGIPGRFTKEAGTLAYYEICDFLRGATVHRILGQQVPYATKGNQWVGYDDQESVKSKVQYLKDRQLAGAMVWALDLDDFQGSFCGQDLRFPLTNAIKDALAAT chi3l1-lgals3-3 3364904 N/A 3364910 unspecified role unspecified role bait bait purified purified c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. N/A 3364911 unspecified role unspecified role prey prey purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false figure 9A chi3l1-lgals3-4 3364904 N/A 3364912 unspecified role unspecified role bait bait partially purified partially purified c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. N/A 3364911 unspecified role unspecified role prey prey purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false figure 9B il13ra2-lgals3-1 3364904 N/A 3364912 unspecified role unspecified role bait bait partially purified partially purified c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. N/A 3364911 inhibitor inhibitor unspecified role unspecified role purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3364913 unspecified role unspecified role prey prey purified purified association association false false false figure 9C-2 il13ra2-tmem219-1 3364904 N/A 3364912 unspecified role unspecified role bait bait partially purified partially purified c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. N/A 3364913 unspecified role unspecified role prey prey purified purified physical association physical association false false false figure 9C-1 567etyu-1 3364905 N/A 3364914 unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3364915 unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3364916 unspecified role unspecified role bait bait cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level association association false false false Figure 8 A/C IL13-P20109 lgals3-il13ra2-1 3364905 N/A 3364915 unspecified role unspecified role bait bait cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3364916 unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false Figure 8Ai IL13-P20109 lgals3-chi3l1-1 3364905 N/A 3364914 unspecified role unspecified role bait bait cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3364916 unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false Figure 8C IL13-P20109 1234qwer-1 3364906 N/A 3364917 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed cell lysate cell lysate N/A 3364911 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Figure 8B chi3l1-lgals3-1 3364907 N/A 3364917 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate N/A 3364911 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed cell lysate cell lysate region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Figure 8Bi 345erttuyu-1 3364908 N/A 3364914 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell N/A 3364916 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell colocalization colocalization false false false Figure 8D Gal-3 and CHI3L1 were similarly expressed in the cytoplasm and to a lesser degree in the nuclei of the lung cells. chi3l1-lgals3-2 3364909 N/A 3364914 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell N/A 3364916 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell colocalization colocalization false false false Figure 8E Gal-3 and CHI3L1 were similarly expressed in the cytoplasm and to a lesser degree in the nuclei of the lung cells.