IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ cao-2018-2 human-hcc Homo sapiens hepatocellular carcinoma cell line hcc hepatocellular carcinoma cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Cao MQ., You AB., Zhu XD., Zhang W., Zhang YY., Zhang SZ., Zhang KW., Cai H., Shi WK., Li XL., Li KS., Gao DM., Ma DN., Ye BG., Wang CH., Qin CD., Sun HC., Zhang T., Tang ZY. sun.huichuan@zs-hospital.sh.cn Journal of hematology & oncology 2018 Only protein-protein interactions imex curation imex curation cao-2018-1 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. mirna interference luciferase assay miRNA interference luciferase reporter assay miRNA interference luciferase assay tag luciferase tag visualisation by luciferase assay tag luciferase Cao MQ., You AB., Zhu XD., Zhang W., Zhang YY., Zhang SZ., Zhang KW., Cai H., Shi WK., Li XL., Li KS., Gao DM., Ma DN., Ye BG., Wang CH., Qin CD., Sun HC., Zhang T., Tang ZY. sun.huichuan@zs-hospital.sh.cn Journal of hematology & oncology 2018 Only protein-protein interactions imex curation imex curation itf2_human Transcription factor 4 Immunoglobulin transcription factor 2 SL3-3 enhancer factor 2 ITF2 SEF2 TCF4 BHLHB19 Class B basic helix-loop-helix protein 19 protein protein human Homo sapiens Human MHHQQRMAALGTDKELSDLLDFSAMFSPPVSSGKNGPTSLASGHFTGSNVEDRSSSGSWGNGGHPSPSRNYGDGTPYDHMTSRDLGSHDNLSPPFVNSRIQSKTERGSYSSYGRESNLQGCHQQSLLGGDMDMGNPGTLSPTKPGSQYYQYSSNNPRRRPLHSSAMEVQTKKVRKVPPGLPSSVYAPSASTADYNRDSPGYPSSKPATSTFPSSFFMQDGHHSSDPWSSSSGMNQPGYAGMLGNSSHIPQSSSYCSLHPHERLSYPSHSSADINSSLPPMSTFHRSGTNHYSTSSCTPPANGTDSIMANRGSGAAGSSQTGDALGKALASIYSPDHTNNSFSSNPSTPVGSPPSLSAGTAVWSRNGGQASSSPNYEGPLHSLQSRIEDRLERLDDAIHVLRNHAVGPSTAMPGGHGDMHGIIGPSHNGAMGGLGSGYGTGLLSANRHSLMVGTHREDGVALRGSHSLLPNQVPVPQLPVQSATSPDLNPPQDPYRGMPPGLQGQSVSSGSSEIKSDDEGDENLQDTKSSEDKKLDDDKKDIKSITSNNDDEDLTPEQKAEREKERRMANNARERLRVRDINEAFKELGRMVQLHLKSDKPQTKLLILHQAVAVILSLEQQVRERNLNPKAACLKRREEEKVSSEPPPLSLAGPHPGMGDASNHMGQM ctnb1_human Catenin beta-1 Beta-catenin OK/SW-cl.35 PRO2286 CTNNB1 CTNNB protein protein human Homo sapiens Human MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPSLSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLDEGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDEDQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLSHHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQKMVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTTSRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGMEGLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDREDITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNLALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGALHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEGATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETADLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGLPDLGHAQDLMDGLPPGDSNQLAWFDTDL foxo3_human Forkhead box protein O3 FOXO3 FKHRL1 FOXO3A Forkhead in rhabdomyosarcoma-like 1 AF6q21 protein protein protein human Homo sapiens Human MAEAPASPAPLSPLEVELDPEFEPQSRPRSCTWPLQRPELQASPAKPSGETAADSMIPEEEDDEDDEDGGGRAGSAMAIGGGGGSGTLGSGLLLEDSARVLAPGGQDPGSGPATAAGGLSGGTQALLQPQQPLPPPQPGAAGGSGQPRKCSSRRNAWGNLSYADLITRAIESSPDKRLTLSQIYEWMVRCVPYFKDKGDSNSSAGWKNSIRHNLSLHSRFMRVQNEGTGKSSWWIINPDGGKSGKAPRRRAVSMDNSNKYTKSRGRAAKKKAALQTAPESADDSPSQLSKWPGSPTSRSSDELDAWTDFRSRTNSNASTVSGRLSPIMASTELDEVQDDDAPLSPMLYSSSASLSPSVSKPCTVELPRLTDMAGTMNLNDGLTENLMDDLLDNITLPPSQPSPTGGLMQRSSSFPYTTKGSGLGSPTSSFNSTVFGPSSLNSLRQSPMQTIQENKPATFSSMSHYGNQTLQDLLTSDSLSHSDVMMTQSDPLMSQASTAVSAQNSRRNVMLRNDPMMSFAAQPNQGSLVNQNLLHHQHQTQGALGGSRALSNSVSNMGLSESSSLGSAKHQQQSPVSQSMQTLSDSLSGSSLYSTSANLPVMGHEKFPSDLDLDMFNGSLECDMESIIRSELMDADGLDFNFDSLISTQNVVGLNVGNFTGAKQASSQSWVPG E5B4E830665A9982 hsa-mir-182-5p Homo sapiens (human) microRNA hsa-miR-182-5p mirna micro rna micro RNA micro-RNA micro-rna mirna miRNA human Homo sapiens Human UUUGGCAAUGGUAGAACUCACACU mrna_foxo 3a mRNA forkhead box O3 mrna messenger rna mRNA mrna human Homo sapiens Human GCGCGAGGCCGTCGATTCGCTCGCGGCTCCATCGCGGCCTGGCCGGGGGGCGGTGTCTGCTGCGCCAGGTTCGCTGGCCGCACGTCTTCAGGTCCTCCTGTTCCTGGGAGGCGGGCGCGGCAGGACTGGGAGGTGGCGGCAGCGGGCGAGGACTCGCCGAGGACGGGGCTCCGGCCCGGGATAACCAACTCTCCTTCTCTCTTCTTTGGTGCTTCCCCAGGCGGCGGCGGCGGCGCCCGGGAGCCGGAGCCTTCGCGGCGTCCACGTCCCTCCCCCGCTGCACCCCGCCCCGGCGCGAGAGGAGAGCGCGAGAGCCCCAGCCGCGGGCGGGCGGGCGGCGAAGATGGCAGAGGCACCGGCTTCCCCGGCCCCGCTCTCTCCGCTCGAAGTGGAGCTGGACCCGGAGTTCGAGCCCCAGAGCCGTCCGCGATCCTGTACGTGGCCCCTGCAAAGGCCGGAGCTCCAAGCGAGCCCTGCCAAGCCCTCGGGGGAGACGGCCGCCGACTCCATGATCCCCGAGGAGGAGGACGATGAAGACGACGAGGACGGCGGGGGACGGGCCGGCTCGGCCATGGCGATCGGCGGCGGCGGCGGGAGCGGCACGCTGGGCTCCGGGCTGCTCCTTGAGGACTCGGCCCGGGTGCTGGCACCCGGAGGGCAAGACCCCGGGTCTGGGCCAGCCACCGCGGCGGGCGGGCTGAGCGGGGGTACACAGGCGCTGCTGCAGCCTCAGCAACCGCTGCCACCGCCGCAGCCGGGGGCGGCTGGGGGCTCCGGGCAGCCGAGGAAATGTTCGTCGCGGCGGAACGCCTGGGGAAACCTGTCCTACGCGGACCTGATCACCCGCGCCATCGAGAGCTCCCCGGACAAACGGCTCACTCTGTCCCAGATCTACGAGTGGATGGTGCGTTGCGTGCCCTACTTCAAGGATAAGGGCGACAGCAACAGCTCTGCCGGCTGGAAGAACTCCATCCGGCACAACCTGTCACTGCATAGTCGATTCATGCGGGTCCAGAATGAGGGAACTGGCAAGAGCTCTTGGTGGATCATCAACCCTGATGGGGGGAAGAGCGGAAAAGCCCCCCGGCGGCGGGCTGTCTCCATGGACAATAGCAACAAGTATACCAAGAGCCGTGGCCGCGCAGCCAAGAAGAAGGCAGCCCTGCAGACAGCCCCCGAATCAGCTGACGACAGTCCCTCCCAGCTCTCCAAGTGGCCTGGCAGCCCCACGTCACGCAGCAGTGATGAGCTGGATGCGTGGACGGACTTCCGTTCACGCACCAATTCTAACGCCAGCACAGTCAGTGGCCGCCTGTCGCCCATCATGGCAAGCACAGAGTTGGATGAAGTCCAGGACGATGATGCGCCTCTCTCGCCCATGCTCTACAGCAGCTCAGCCAGCCTGTCACCTTCAGTAAGCAAGCCGTGCACGGTGGAACTGCCACGGCTGACTGATATGGCAGGCACCATGAATCTGAATGATGGGCTGACTGAAAACCTCATGGACGACCTGCTGGATAACATCACGCTCCCGCCATCCCAGCCATCGCCCACTGGGGGACTCATGCAGCGGAGCTCTAGCTTCCCGTATACCACCAAGGGCTCGGGCCTGGGCTCCCCAACCAGCTCCTTTAACAGCACGGTGTTCGGACCTTCATCTCTGAACTCCCTACGCCAGTCTCCCATGCAGACCATCCAAGAGAACAAGCCAGCTACCTTCTCTTCCATGTCACACTATGGTAACCAGACACTCCAGGACCTGCTCACTTCGGACTCACTTAGCCACAGCGATGTCATGATGACACAGTCGGACCCCTTGATGTCTCAGGCCAGCACCGCTGTGTCTGCCCAGAATTCCCGCCGGAACGTGATGCTTCGCAATGATCCGATGATGTCCTTTGCTGCCCAGCCTAACCAGGGAAGTTTGGTCAATCAGAACTTGCTCCACCACCAGCACCAAACCCAGGGCGCTCTTGGTGGCAGCCGTGCCTTGTCGAATTCTGTCAGCAACATGGGCTTGAGTGAGTCCAGCAGCCTTGGGTCAGCCAAACACCAGCAGCAGTCTCCTGTCAGCCAGTCTATGCAAACCCTCTCGGACTCTCTCTCAGGCTCCTCCTTGTACTCAACTAGTGCAAACCTGCCCGTCATGGGCCATGAGAAGTTCCCCAGCGACTTGGACCTGGACATGTTCAATGGGAGCTTGGAATGTGACATGGAGTCCATTATCCGTAGTGAACTCATGGATGCTGATGGGTTGGATTTTAACTTTGATTCCCTCATCTCCACACAGAATGTTGTTGGTTTGAACGTGGGGAACTTCACTGGTGCTAAGCAGGCCTCATCTCAGAGCTGGGTGCCAGGCTGAAGGATCACTGAGGAAGGGGAAGTGGGCAAAGCAGACCCTCAAACTGACACAAGACCTACAGAGAAAACCCTTTGCCAAATCTGCTCTCAGCAAGTGGACAGTGATACCGTTTACAGCTTAACACCTTTGTGAATCCCACGCCATTTTCCTAACCCAGCAGAGACTGTTAATGGCCCCTTACCCTGGGTGAAGCACTTACCCTTGGAACAGAACTCTAAAAAGTATGCAAAATCTTCCTTGTACAGGGTGGTGAGCCGCCTGCCAGTGGAGGACAGCACCCCTCAGCACCACCCACCCTCATTCAGAGCACACCGTGAGCCCCCGTCGGCCATTCTGTGGTGTTTTAATATTGCGATGGTTTATGGGACGTTTTAAGTGTTGTTCTTGTGTTTGTTTTCCTTTGACTTTCTGAGTTTTTCACATGCATTAACTTGCGGTATTTTTCTGTTAAAATGTTAACCGTCCTTCCCCTAGCAAATTTAAAAACAGAAAGAAAATGTTGTACCAGTTACCATTCCGGGTTCGAGCATCACAAGCTTTTGAGCGCATGGAACTCCATAAACTAACAAATTACATAAACTAAAGGGGGATTTTCTTTCTTCTTTTGTTTGGTAGAAAATTATCCTTTTCTAAAAACTGAACAATGGCACAATTGTTTGCTATGTGCACCCGTCCAGGACAGAACCGTGCATAGGCAAAAGGAGTGGAGCACAGCGTCCGGCCCAGTGTGTTTCCGGTTCTGAGTCAGGGTGATCTGTGGACGGGACCCCAGCACCAAGTCTACGGGTGCCAGATCAGTAGGGCCTGTGATTTCCTGTCAGTGTCCTCAGCTAATGTGAACAGTGTTGGTCTGCTGGTTAGAAACTAGAATATTGATATTTTCAGGAAAGAAATCAGCTCAGCTCTCCACTCATTGCCAAATGTCACTAAAGGGTTTAGTTTTAAGGAGAAAGAAAAGGAAAAAAAAAAAAAACAAAAAAGTCCTGTTTTGCTTTGCAGAACAAATGAACTTACAGGTGAGCATTAAGCTTGCAGTGAGAAATGTGCGAAGAGTAAAAACCCAAGTCAATGCTGAGGCAGTTCTAACTTCACTGTTTTCCTAAATACACATCCTTGATTATTTTCAGCCTTGCTATATAATCTGATCTGCTAGAAGTGTATGAGTGAGAGGCAATAGCATACAAACTGATTTTTTAAATATAAGCTTAGGTTGTAATTGTACAAGTGACTCAATGGAAGTACAAAATAGGGCAGTTTTAACTTTTTTTTCTGCTTCTATGGATTTCATTTTGTTGTGTTTTCAAAAAGTTATGGTGCTGTATAGGTGCTTTCTGTTTAACCTGGAAAGTGTGATTATATTCGTTACCTTCTTTGGTAGACGGAATAGTTGGGACCACCTTTGGTACATAAGAAATTGGTATAACGATGCTCTGATTAGCACAGTATATGCATACTTCTCCAAAGTGATATATGAAGACTCTTTTCTTTGCATAAAAAGCATTAGGCATATAAATGTATAAATATATTTTATCATGTACAGTACAAAAATGGAACCTTATGCATGGGCCTTAGGAATACAGGCTAGTATTTCAGCACAGACTTCCCTGCTTGAGTTCTTGCTGATGCTTGCACCGTGACAGTGGGCACCAACACAGACGTGCCACCCAACCCCCTGCACACACCACCGGCCACCAGGGGCCCCCTTGTGCGCCTTGGCTTTATAACTCCTCTGGGGGTGATATTGGTGGTGATCACAGCTCCTAGCATAATGAGAGTTCCATTTGGTATTGTCACACGTCTCCTGCCTCGCTTGGGTTGCCATGTTTGAGCGATGGCCCTGTTGATTTCACCCTGCCTTTTACTGAATCTGTAAATTGTTGTGCAATTGTGGTTATAGTAGACTGTAGCACATTGCCTTTTCTAAACTGCTACATGTTTATAATCTTCATTTTTAAAGTATGTGTAATTTTTTTAAGTATGTATTCTATTCATATGGTCTGCTTGTCAGTGAGCCAGACTTGCTTACTATATTCCTTTATAATAATGCTAGCCACTTCCTGGATTCTTTAGTAATGTGCTGTATGCAAGAACTTTCCAGTAGCAGTGAAGGAGGGTTGCCTCTCCAAGCTTCCTAAGGGATGCTGCCCTGTGTGGGGATGCATTGCAGAGGCACTAGTAGCATGGGGGCTAGAGTGGGGAGCGAGATGTAAAAGGGTGGGGGGATAGGAGAATTCCAGAGTGCTTCCAGCATTAGGGTCCTGAGAACTTCTGAGTTCAGAGAAACATGCAAAGTGACTAACAAAATAGCTACTTACCTTTGCAGTTTTACAGACCCTGGGAGCTGCTTTGGGAGTGAGAAAGGCAACCCTCCAATGTGTTTCAACTTTAAAATGTTGAATTCTTTTCAGACATGGTATCTCATTTATTCTCCTTTTCTAGCGTTTGTTGAATTTCAGGCAGAATGTCTTACAGAATGTCCTAGAACCAGATTATCATTTAATCTGAAACAGCTGAGGAAGGGACAGAGAAGGTACAAGGGCAAGGCAGCACAAAACAGATCAGGAGAATGAAGAGGGAATGCTTTGGTTTTTTGTTTTGTTTTGTTTTTTCTTTTTCAAGTAACTAAAACAGCATCTACATGTAGAGTGTTGTGGAGAGCTGAGACCAGGGTAAAGTCAAGTGCAGCATCAGTACTGCGAGACCCACCAGCCCCTGGAGAGGGTCAGCCGAGAATCTGGTAGTGAAGCCTGTCTAGGGTCCCGGCACCCTCACCCTCAGCCACCTGCAGAGAGGCCAGGGCCCCAGAGACTAGCCTGGTTCTGAAGTGGGCAGGGGTGCTGCCAGAGCCCTCTGCCCCTTATGTTGAGACCCTGCTTTCAGGACAGGCCAGCCGTTGGCCACCATGTCACATTCTGAGTGAGTGTCACAGGTCCCTAACAATAATTTTCTGATCTGGAGCATATCAGCAGAATGCTTAGCCTCAAGGGGCCTGGCAGCTGTAATGTTTGATTTATGATGAGAACTATCCGAGGCCACCCTTGGCCTCTAAATAAGCTGCTCTAGGGAGCCGCCTACTTTTTGATGAGAAATTAGAAGAGTACCTAATGTTGAAAACATGACATGCGCTCTTGGGATCTGCTGTTCTCTCCAGGGCTCCAGAACCTGATACCTGTTACCAAAGCTAGGAAAGAGCTTTATCACAAGCCTTCACTGTCCTGGCATGAGAACTGGCTGCCAGGCTCAGTGTACCCCATTAACTGTGAATGAATCTGAGCTTGGTTTCCTTTATTGCTTCCTCTGCAATATGATTGCTGAAACACATTTTAAAAATTCAGAAGCTTGTCACTCCTGTTAATGGGAGGATCAGTCACACATGTGTAGTACAAGGCGGACTTTGTGTTTGTTTTTGGTGTTAATTTTTAGCATTGTGTGTGTTGCTTCCCCACCCTGAGGAGAGGACACCATGGCTTACTACTCAGGACAAGTATGCCCCGCTCAGGGTGTGATTTCAGGTGGCTTCCAAACTTGTACGCAGTTTAAAGATGGTGGGGACAGACTTTGCCTCTACCTAGTGAACCCCACTTAAAGAATAAGGAGCATTTGAATCTCTTGGAAAAGGCCATGAAGAATAAAGCAGTCAAAAAGAAGTCCTCCATGTTGGTGCCAAGGACTTGCGAGGGGAAATAAAAATGTTATCCAGCCTGACCAACATGGAGAAACCCCGTCTCCATTAAAAATACAAAATTAGCCTGGCATGGTGGCGCATGCCTGTAATCCCAGCTACTCTGGAGGCTGAGGCAGGAGAATCGCTTGAACCCAGGAGGCGGAGGTCGCAGTGAGCCGAGATCATGCCAGTGCACTCCAGCCTGGGTAACAAGAGTGAAACTCCGTGTCAAAAAAAAAAAAAAAATGTTACTCATCCTCTCTGAAAGCAAAAAGGAAACCCTAACAGCTCTGAACTCTGGTTTTATTTTTCTTGCTGTATTTGGGTGAACATTGTATGATTAGGCATAATGTTAAAAAAAAAAATTTTTTTTTGGTAGAAATGCAATCACCAGTAAAGAGGTACGAAAAAGCTAGCCTCTCTCAGAGACCGGGGAGGCAGAGTACTACTAGAGGAAGTGAAGTTCTGATGGAATCATGCCTGTCAAATGAGGTCTTGAAGCGGATGCCCAAATAAAAGAGTATATTTTATCTAAATCTTAAGTGGGTAACATTTTATGCAGTTTAAATGAATGGAATATTTTCCTCTTGTTTAGTTGTATCTGTTTGTATTTTTCTTTGATGAATGATTGGTCATGAGGCCTCTTGCCACACTCCAGAAATACGTGTGCGGCTGCTTTTAAGAACTATGTGTCTGGTCACTTATTTCTCTAAAATTATCTCATTGCCTGGCAATCAGTCTTCTCTTGTATACTTGTCCTAGCACATTATGTACATGGGAAATGTAAACAAATGTGAAGGAGGACCAGAAAAATTAGTTAATATTTAAAAAAATGTATTGTGCATTTTGGCTTCACATGTTTAACTTTTTTTAAGAAAAAAGTTGCATGAATGGAAAAAAAAATCTGTATACAGTATCTGTAAAAACTATCTTATCTGTTTCAATTCCTTGCTCATATCCCATATAATCTAGAACTAAATATGGTGTGTGGCCATATTTAAACACCTGAGAGTCAAGCAGTTGAGACTTTGATTTGAAGCACCTCATCCTTCTTTCAATGCGAACACTATCATATGGCATTCTTACTGAGGATTTTGTCTAACCATATGTTGCCATGAATTAACTCTGCCGCCTTTCTTAAGGATCAAAACCAGTTTGATTTGGGAATCTTCCCCTTTCCAAATGAAATAGAGATGCAGTACTTAACTTTCCTTGGTGTTTGTAGATATTGCCTTGTGTATTCCACTTAAAACCGTAATCTAGTTTGTAAAAGAGATGGTGACGCATGTAAATAAAGCATCAGTGACACTCT tcf4-ctnnb1-13 3432975 N/A 3432977 unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3432978 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false Fig6b tcf4-ctnnb1-12 3432975 N/A 3432978 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3432979 unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false Fig6c hsa_mir_182 5p-mrna_foxo 3a-1 3432976 N/A 3432980 predetermined predetermined participant predetermined regulator regulator modulator regulator neutral component neutral component over-expressed over expressed level over-expressed living cell living cell engineered engineered N/A 3432981 regulator target regulator target regulator target modulator target neutral component neutral component over-expressed over expressed level over-expressed living cell living cell engineered engineered r.[2437_2444delinsaacggttt; 3279_3286delinsaacggttt] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false r.3279_3286delinsaacggttt mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false tag luciferase tag luciferase tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false r.2437_2444delinsaacggttt mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false FIG4A