IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ moura-2018-7 in vitro In vitro protein kinase assay protein kinase assay western blot western blot Immuno blot Immuno blot Moura DS, Campillo‐Marcos I, Vázquez‐Cedeira M, Lazo PA, Moura DS., Campillo-Marcos I., Vázquez-Cedeira M., Lazo PA. Details about construct details and cell lines were provided by the authors upon email request. Especifically, the author confirmed that the AURKB constructs were of human origin. pedro.lazo@csic.es Cellular and molecular life sciences : CMLS 2018 Only protein-protein interactions imex curation imex curation moura-2018-6 in vitro In vitro protein kinase assay protein kinase assay autoradiography autoradiography Moura DS, Campillo‐Marcos I, Vázquez‐Cedeira M, Lazo PA, Moura DS., Campillo-Marcos I., Vázquez-Cedeira M., Lazo PA. Details about construct details and cell lines were provided by the authors upon email request. Especifically, the author confirmed that the AURKB constructs were of human origin. pedro.lazo@csic.es Cellular and molecular life sciences : CMLS 2018 Only protein-protein interactions imex curation imex curation moura-2018-2 human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Moura DS, Campillo‐Marcos I, Vázquez‐Cedeira M, Lazo PA, Moura DS., Campillo-Marcos I., Vázquez-Cedeira M., Lazo PA. Details about construct details and cell lines were provided by the authors upon email request. Especifically, the author confirmed that the AURKB constructs were of human origin. pedro.lazo@csic.es Cellular and molecular life sciences : CMLS 2018 Only protein-protein interactions imex curation imex curation moura-2018-8 human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Moura DS, Campillo‐Marcos I, Vázquez‐Cedeira M, Lazo PA, Moura DS., Campillo-Marcos I., Vázquez-Cedeira M., Lazo PA. Details about construct details and cell lines were provided by the authors upon email request. Especifically, the author confirmed that the AURKB constructs were of human origin. pedro.lazo@csic.es Cellular and molecular life sciences : CMLS 2018 Only protein-protein interactions imex curation imex curation moura-2018-1 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Moura DS, Campillo‐Marcos I, Vázquez‐Cedeira M, Lazo PA, Moura DS., Campillo-Marcos I., Vázquez-Cedeira M., Lazo PA. Details about construct details and cell lines were provided by the authors upon email request. Especifically, the author confirmed that the AURKB constructs were of human origin. pedro.lazo@csic.es Cellular and molecular life sciences : CMLS 2018 Only protein-protein interactions imex curation imex curation moura-2018-3 human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Moura DS, Campillo‐Marcos I, Vázquez‐Cedeira M, Lazo PA, Moura DS., Campillo-Marcos I., Vázquez-Cedeira M., Lazo PA. Details about construct details and cell lines were provided by the authors upon email request. Especifically, the author confirmed that the AURKB constructs were of human origin. pedro.lazo@csic.es Cellular and molecular life sciences : CMLS 2018 Only protein-protein interactions imex curation imex curation moura-2018-4 human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference confocal microscopy confocal microscopy immunostaining immunostaining Moura DS, Campillo‐Marcos I, Vázquez‐Cedeira M, Lazo PA, Moura DS., Campillo-Marcos I., Vázquez-Cedeira M., Lazo PA. Details about construct details and cell lines were provided by the authors upon email request. Especifically, the author confirmed that the AURKB constructs were of human origin. pedro.lazo@csic.es Cellular and molecular life sciences : CMLS 2018 Only protein-protein interactions imex curation imex curation moura-2018-5 human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 confocal microscopy confocal microscopy immunostaining immunostaining Moura DS, Campillo‐Marcos I, Vázquez‐Cedeira M, Lazo PA, Moura DS., Campillo-Marcos I., Vázquez-Cedeira M., Lazo PA. Details about construct details and cell lines were provided by the authors upon email request. Especifically, the author confirmed that the AURKB constructs were of human origin. pedro.lazo@csic.es Cellular and molecular life sciences : CMLS 2018 Only protein-protein interactions imex curation imex curation h31t_human Histone H3.1t H3-4 H3/g H3FT HIST3H3 Histone H3.4 protein protein human Homo sapiens Human MARTKQTARKSTGGKAPRKQLATKVARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLMREIAQDFKTDLRFQSSAVMALQEACESYLVGLFEDTNLCVIHAKRVTIMPKDIQLARRIRGERA vrk1_human Serine/threonine-protein kinase VRK1 Vaccinia-related kinase 1 VRK1 protein protein human Homo sapiens Human MPRVKAAQAGRQSSAKRHLAEQFAVGEIITDMAKKEWKVGLPIGQGGFGCIYLADMNSSESVGSDAPCVVKVEPSDNGPLFTELKFYQRAAKPEQIQKWIRTRKLKYLGVPKYWGSGLHDKNGKSYRFMIMDRFGSDLQKIYEANAKRFSRKTVLQLSLRILDILEYIHEHEYVHGDIKASNLLLNYKNPDQVYLVDYGLAYRYCPEGVHKEYKEDPKRCHDGTIEFTSIDAHNGVAPSRRGDLEILGYCMIQWLTGHLPWEDNLKDPKYVRDSKIRYRENIASLMDKCFPEKNKPGEIAKYMETVKLLDYTEKPLYENLRDILLQGLKAIGSKDDGKLDLSVVENGGLKAKTITKKRKKEIEESKEPGVEDTEWSNTQTEEAIQTRSRTRKRVQK aurkb_human Aurora kinase B AIRK2 Aurora- and IPL1-like midbody-associated protein 1 Aurora/IPL1-related kinase 2 STK-1 Serine/threonine-protein kinase 12 Serine/threonine-protein kinase 5 STK1 STK5 Aurora 1 AURKB AIK2 AIM1 ARK2 STK12 Serine/threonine-protein kinase aurora-B protein protein human Homo sapiens Human MAQKENSYPWPYGRQTAPSGLSTLPQRVLRKEPVTPSALVLMSRSNVQPTAAPGQKVMENSSGTPDILTRHFTIDDFEIGRPLGKGKFGNVYLAREKKSHFIVALKVLFKSQIEKEGVEHQLRREIEIQAHLHHPNILRLYNYFYDRRRIYLILEYAPRGELYKELQKSCTFDEQRTATIMEELADALMYCHGKKVIHRDIKPENLLLGLKGELKIADFGWSVHAPSLRRKTMCGTLDYLPPEMIEGRMHNEKVDLWCIGVLCYELLVGNPPFESASHNETYRRIVKVDLKFPASVPMGAQDLISKLLRHNPSERLPLAQVSAHPWVRANSRRVLPPSALQSVA p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD vrk1-11 3337991 N/A 3337999 enzyme target enzyme target substrate substrate neutral component neutral component purified purified resulting phosphorylated residue opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Commercial preparation from Millipore (ref. 14-494). N/A 3338000 enzyme enzyme neutral component neutral component engineered engineered purified purified n-terminal region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation false false false 3c, 3d, S4 hist3h3-vrk1-1 3337991 N/A 3337999 enzyme target enzyme target substrate substrate neutral component neutral component purified purified resulting phosphorylated residue opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Commercial preparation from Millipore (ref. 14-494). N/A 3338001 enzyme enzyme neutral component neutral component engineered engineered purified purified n-terminal region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation false false false 3b, 3d,S4 hist3h3-vrk1-2 3337991 N/A 3338002 enzyme target enzyme target substrate substrate neutral component neutral component engineered engineered purified purified resulting phosphorylated residue opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt certain certain sequence position certain certain certain sequence position certain false n-terminal region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3338000 enzyme enzyme neutral component neutral component engineered engineered purified purified n-terminal region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation false false false 3e aurkb-hist3h3-3 3337991 N/A 3338000 inhibitor inhibitor neutral component neutral component purified purified n-terminal region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q99986:p.Lys179Glu mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3337999 enzyme target enzyme target substrate substrate neutral component neutral component purified purified resulting phosphorylated residue opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Commercial preparation from Millipore (ref. 14-494). N/A 3338001 enzyme enzyme neutral component neutral component engineered engineered purified purified n-terminal region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation false false false 3b, S4 tp53-vrk1-4 3337991 N/A 3338001 inhibitor inhibitor neutral component neutral component purified purified n-terminal region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q96GD4:p.Lys106Arg mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3338002 enzyme target enzyme target substrate substrate neutral component neutral component engineered engineered purified purified resulting phosphorylated residue opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt certain certain sequence position certain certain certain sequence position certain false n-terminal region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3338000 enzyme enzyme neutral component neutral component engineered engineered purified purified n-terminal region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation false false false 3e hist3h3-vrk1-3 3337991 N/A 3338001 inhibitor inhibitor neutral component neutral component purified purified n-terminal region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q96GD4:p.Lys106Arg mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3337999 enzyme target enzyme target substrate substrate neutral component neutral component purified purified resulting phosphorylated residue opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Commercial preparation from Millipore (ref. 14-494). N/A 3338000 enzyme enzyme neutral component neutral component engineered engineered purified purified n-terminal region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation false false false 3c, S4 aurkb-hist3h3-4 3337991 N/A 3338000 inhibitor inhibitor neutral component neutral component purified purified n-terminal region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3337999 enzyme target enzyme target substrate substrate neutral component neutral component purified purified resulting phosphorylated residue opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Commercial preparation from Millipore (ref. 14-494). N/A 3338001 enzyme enzyme neutral component neutral component engineered engineered purified purified n-terminal region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation false false false 3bi There is an inverse correlation between H3–S11 phosphorylation and H3–T4 phosphorylation as the ratio of AURKB/VRK1 changed. aurkb-hist3h3-5 3337991 N/A 3338001 inhibitor inhibitor neutral component neutral component purified purified n-terminal region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3337999 enzyme target enzyme target substrate substrate neutral component neutral component purified purified resulting phosphorylated residue opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Commercial preparation from Millipore (ref. 14-494). N/A 3338000 enzyme enzyme neutral component neutral component engineered engineered purified purified n-terminal region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation false false false 3c aurkb-hist3h3-1 3337992 N/A 3338001 enzyme enzyme neutral component neutral component engineered engineered purified purified n-terminal region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q96GD4:p.Lys106Arg mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3337999 enzyme target enzyme target substrate substrate neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Commercial preparation from Millipore (ref. 14-494). phosphorylation phosphorylation reaction phosphorylation false false false 3a vrk1-10 3337992 N/A 3338000 enzyme enzyme putative self putative self engineered engineered purified purified n-terminal region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q99986:p.Lys179Glu mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation false true false 3a, S3a tp53-vrk1-2 3337992 N/A 3338002 enzyme target enzyme target substrate substrate neutral component neutral component engineered engineered purified purified n-terminal region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3338000 enzyme enzyme neutral component neutral component engineered engineered purified purified n-terminal region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation false false false S3a aurkb-hist3h3-2 3337992 N/A 3338000 inhibitor inhibitor neutral component neutral component purified purified n-terminal region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q99986:p.Lys179Glu mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3338001 enzyme enzyme neutral component neutral component engineered engineered purified purified n-terminal region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q96GD4:p.Lys106Arg mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3337999 enzyme target enzyme target substrate substrate neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Commercial preparation from Millipore (ref. 14-494). phosphorylation phosphorylation reaction phosphorylation false false false 3a, S3b vrk1-12 3337992 N/A 3338001 inhibitor inhibitor neutral component neutral component purified purified n-terminal region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q96GD4:p.Lys106Arg mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3338000 enzyme enzyme putative self putative self engineered engineered purified purified n-terminal region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q99986:p.Lys179Glu mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation false false false 3a aurkb-vrk1-2 3337993 N/A 3338001 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed engineered engineered n-terminal region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 3338000 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed engineered engineered n-terminal region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q99986:p.Lys179Glu mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 1b vrk1-aurkb-1 3337993 N/A 3338001 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed engineered engineered n-terminal region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 3338000 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed engineered engineered n-terminal region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q99986:p.Lys179Glu mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 1c aurkb-vrk1-6 3337994 N/A 3338001 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3338000 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false 4b Maximum strengh of this interaction was depected 360 minutes after nocodazole release. aurkb-vrk1-7 3337994 N/A 3338001 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3337999 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level phosphorylated residue opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt certain certain sequence position certain certain certain sequence position certain false phosphorylated residue opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 7b VRK1 (Q99986), nocodazole (CHEBI:34892) Phosphorylation of Thr4 and Ser11 in H3 decreased in absence of VRK1. aurkb-vrk1-1 3337995 N/A 3338001 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate engineered engineered n-terminal region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. N/A 3338000 unspecified role unspecified role bait bait purified purified n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q99986:p.Lys179Glu mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false 1a aurkb-vrk1-3 3337996 N/A 3338001 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3338000 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false 1d, 1e, 1f Interaction detected also in serum-deprived cells, during mitosis and faintly in asynchronous phase. aurkb-vrk1-4 3337997 N/A 3338001 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell N/A 3338000 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell colocalization colocalization false false false 2, 7d Only a minor colocalization of VRK1 and AURKB is detectable in anaphase in the central spindle. Nocodazole (CHEBI:34892) aurkb-vrk1-5 3337998 N/A 3338001 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell N/A 3338000 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell colocalization colocalization false false false S2 The interaction is only detected after treatment with thymidine + nocodazole, which arrests cell cycle at early mitosis/G2 phase.