IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ varendi-2014-1 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. mirna interference luciferase assay miRNA interference luciferase reporter assay miRNA interference luciferase assay tag luciferase tag visualisation by luciferase assay tag luciferase Varendi K., Kumar A., Härma MA., Andressoo JO., Varendi K., Kumar A., Härma MA., Andressoo JO. jaan-olle.andressoo@helsinki.fi jaan‑olle.andressoo@helsinki.fi Cellular and molecular life sciences : CMLS 2014 imex curation Only protein-protein interactions imex curation mmu-mir-1a 3p miR-1a 3p mirna micro rna micro RNA micro-RNA micro-rna mirna miRNA mouse Mus musculus Mouse UGGAAUGUAAAGAAGUAUGUAU mrna_bdnf-1 Brain-derived neurotrophic factor mrna messenger rna mRNA mrna mouse Mus musculus Mouse TAAAGCAGTAGCCGGCTGGTGCAGAAAAGCAACAAGTTCCCCAGCGGTCTTCCCGCCCTAGCTTGACAAGGCGAAGGGTTTCTTACCTGGCGACAGGGAAATCTCCTGAGCCGAGCTCATCTTTGCCAGAGCCCCAGGTGTGACCTGAGCAGTGGGCAAAGGATCGGCGTGCAAATTGGATTATTTTTATGGGGGTACTCTGAAACTCCCTCACTTTCTCTGGGAACTTTTTGTGCTAGGGCTCAGTGACAGGCGTTGAGAAAGCTGCTTCAGGAAACGCCCGCTATATAGCAGGGCAATTGGACAGTCATTGGTAACCTCGCTCATTCATTAGAATCACGTAAGAACTCAAAGGGAAACGTGTCTCTCAGAATGAGGGCGTTTGCGTAAATCTATAGGTTTTTCAACATCGATGCCAGTTGCTTTGTCTTCTGTAGTCGCCAAGGTGGATGAGAGTTGAAGCTTTGCGGATATTGCGAAGGGTTATTAGATTCATAAGTCACACCAAGTGGTGGGCGATCCACTGAGCAAAGCCGAACTTCTCACATGATGACTTCAAACAAGACACATTACCTTCCTGCATCTGTTGGGGAGACAAGATTTTAAGACACTGAGTCTCCAGGACAGCAAAGCCACAATGTTCCACCAGGTGAGAAGAGTGATGACCATCCTTTTCCTTACTATGGTTATTTCATACTTCGGTTGCATGAAGGCGGCGCCCATGAAAGAAGTAAACGTCCACGGACAAGGCAACTTGGCCTACCCAGGTGTGCGGACCCATGGGACTCTGGAGAGCGTGAATGGGCCCAGGGCAGGTTCGAGAGGTCTGACGACGACATCACTGGCTGACACTTTTGAGCACGTCATCGAAGAGCTGCTGGATGAGGACCAGAAGGTTCGGCCCAACGAAGAAAACCATAAGGACGCGGACTTGTACACTTCCCGGGTGATGCTCAGCAGTCAAGTGCCTTTGGAGCCTCCTCTACTCTTTCTGCTGGAGGAATACAAAAATTACCTGGATGCCGCAAACATGTCTATGAGGGTTCGGCGCCACTCCGACCCTGCCCGCCGTGGGGAGCTGAGCGTGTGTGACAGTATTAGCGAGTGGGTCACAGCGGCAGATAAAAAGACTGCAGTGGACATGTCTGGCGGGACGGTCACAGTCCTAGAGAAAGTCCCGGTATCCAAAGGCCAACTGAAGCAGTATTTCTACGAGACCAAGTGTAATCCCATGGGTTACACCAAGGAAGGCTGCAGGGGCATAGACAAAAGGCACTGGAACTCGCAATGCCGAACTACCCAATCGTATGTTCGGGCCCTTACTATGGATAGCAAAAAGAGAATTGGCTGGCGATTCATAAGGATAGACACTTCCTGTGTATGTACACTGACCATTAAAAGGGGAAGATAGTGGATTTATGTTGTATAGATTATATTGAGACAAAATTATCTATTTGTATATATACATAACAGGGTAAATTATTCAGTTAAGAAAAAATAATTTTATGAACTGCATGTATAAATGAAGTTTATACAGTACAGTGGTTCTACAATCTATTTATTGGACATATCCATGACCTGAAAGGAAACAGTCATTTGCGCACAACTTTAAAAGTCTGCATTACATTCCTCGATAATGTTGTGGTTTGTTGCCGTTGCCAAGAATTGAAAACAAAAAGTTTAAAAAAAATAATAATAAATTGCATGCTGCTTTAATTGTGAATTGATAATAAACTGTCCCTCTTTCAGAAAACAGATTAAAAAAACAAAAAACAAAAAAAAAAAAACAAAAAACAAAAACAAAAATTGGAACCAAAACATTCCGTTTACATTTTAGACACTAAGTATCTTCGTTCTTGTTAGTACTCTGTTTTACTGCTTTCGACTTCTGATAGCGTTGGAATTAAAACAATGTCAAGGTGCTGTTGTCATTGCTTTACTGGCGTAAGGGACGGGGAATGGGAGGGGTAGATTTCTGTTTGTTTTGTGTTTTATTTTGTTTGTTTGTTTGTTTTGTTTTTTAGTTCCACCCGGAGTAGGGATGGAGAAAATTTCTTCACTATCCATTCTGGTTGATAAAGCGTTACATTTGTATGTTGTAAAGATGTTTGCAAAATCCAATCAGATGACTGGAAAACAAATAAAAATTAAGGCAACTGAATAAAATGCTCACACTCCACTGCCCATGATGTATCTCCCTGGTCCCCCTCAGCTCACTCTTCTGGCATGGGTCAGGGAAAATTGCTTTTATTGGAAAGACCAGCATTTGTTCAAAGCATACTCTTTCCCTCCCTCCTCCCATTTTGGTCCCTTCTTTTTGTTTTGTTTTAAGAAAGAAAATTAAGTTGCGCGCTTTAAAATATTTTACTACTGCTACAAACAGATGAACAATGTGTGTCATTTTATGACACTCATGGAAAACAGTGATTTTTTTTTACCCTAAAGAAAAACAAATAAAAATAACCCAAAATATTCTTTTTTTAAAAGGCATAAATATTGGGTAAATTGTAATATGGCCTAACAGTGTTTGCAGATAAAAGTTATTGTATACACCCAGATACTTAGATAAGAGCAGGGATCCACACTGCCATTGAAATAGGACTGAATGGCCCTGCGGAGGCTAAGTGGAGCTGACATACTATTTCCTGGCAGTGCAGGAGGAATTTCTGAGTGGCCATCCTAAGGTCTAGGATGGAGGTGGGGAATGGTACTTGAGACATTCCTAAAGGAAGGCTCGGAAGCACCCTTCAGAGCAGGCTCTGGAATGATGTGTCAAGTTGCTTAGGCCTTCTGCTTTAAGTGCCTACATTACCTAACAGTGCTCAAGAGGTTCTCGATTGGAGAACCACACTCAAATCCATTTATAGCCTCCATCCCATTTCTAAATAATTGTGTATAAAGTTGGATTAACCTGGAGCAACTTTGGATCCAAATATGGCACAGCAATAATGATATTAATGCAGCATGATGGGAAATGTTTGCTGTGAAGAGAATTGATTTGCTTTGAGCTTAGACTTCAGGAAGCCTAGGTTTTTTATTTTTTTATTTTTGAGACATTTTGGTAAAAGGAAAAAAAGAAAACAAACAAACAAACAAACAAAACCAGAAAAAGCATCAAAACTCAGGCAGAATGAGCAATGTCTGAAAGGGCTAGAAAAACAAGACATAGCAAGGTGCTTTCACTGTGAAAGAGACAAGAACACAGGAGGAAATATTGCTTCAGTGAAGAGCACAGACGGCTCCTGCCAATTTATTACAAGAGTCCCGTCTGTACTTTACCCTTTGGGGTTAGAAGTCAAGTTGGAAGCCTGAATGAATGGACCCAATGAGAACTAGTGTTAAGCCCATTTCCCTAGTCAGGTTTTTTTCAAGCGTGAATGTGTTAGTGGTTACTCTCCTGGGTTCCTGAGCATCAGAAAAAAAAAAAAAAAGAGGCAAACAATCGCTTCATCTTAGGAGTGGAAAGGAAACAGAAGTGGACGTCCGCTGTGACTCAGGGAGTGAAGATACCATCAGCAAATAGTTTCTTTTTTGTTCATTCGTTCCTTTCGAGTTAGCCTGTCTTTTGGAATACCACTGAATATGCTGTTTTTGAAAGACTTCATGTAGCATAGATTGTTTTGTGCCGTTTACCAAATTAACCTTTGTCATCGTTTTTTAACCTATTCAGGAATGCTTGGAATATCTGCTCTATGTTAACTTTTTGCAGCTTCATTCTGAGAGACATTAGTCAAACAAACAAAAGGATCCCCATCACAATCTTACAGTACTGCAAGGGCCAGGTCTGTTAATCGGCTTCACAGGAGACATCAGCAATTGTGTGGTCAGTGGCTGGCTCTCTTACCCACTAAGATACATCATAGCTACATGTTGGTGGTTTATGTTGACCTGAGATTTATTTGTTAAAATCTCTTCTTCGTTTCTGTTCGTTCTGGTTCTGTTCTGTTCTGTTCTGTTCTGTTTTGGTTTTAAAGTCTTGCTGTGGTCTCTTGTTGGCAGAAATGTTTTATGCATGGCAGCAGGCCTGTTGCTTTTTTATAGTGATTCCCATTGAAACTGTAAGTAAATGTCTGTGGCCTTGTTCTCTCTATGGTAAAGATATTATTCACCATGTAAAACAAGAAAAAATATTTATTGTATTTTAGTATATTTATATAATTATGTTATTGAAAAAATTGGCATTAAAACTTAACCACATCAGAAGCCTATTGTAAATACAGGTTCTATTTAAGTGTACCAATTAACATATAATATATGTTTTAAATATAGAATTTTTAATGTTTTTAAATATATTTTCAAAGT mmu-mir-10b 5p miR-10b 5p mirna micro rna micro RNA micro-RNA micro-rna mirna miRNA mouse Mus musculus Mouse UACCCUGUAGAACCGAAUUUGUG mmu-mir-191 5p miR-191 5p mirna micro rna micro RNA micro-RNA micro-rna mirna miRNA mouse Mus musculus Mouse CAACGGAAUCCCAAAAGCAGCUG mmu-mir-155 5p miR-155 5p mirna micro rna micro RNA micro-RNA micro-rna mirna miRNA mouse Mus musculus Mouse UUAAUGCUAAUUGUGAUAGGGGU mir_1-mrna_bdnf_1-1 3339128 N/A 3339129 predetermined predetermined participant predetermined regulator regulator modulator regulator neutral component neutral component over-expressed over expressed level over-expressed living cell living cell synthetic construct synthetic construct N/A 3339130 regulator target regulator target regulator target modulator target neutral component neutral component over-expressed over expressed level over-expressed living cell living cell engineered engineered tag luciferase tag luciferase tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false r.[1624a>t;1628t>c;1630c>a] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false r.1820_1829delinstagtttcact mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false r.[2717a>t;2721t>c;2723c>a] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig 2b, Fig4c mir_10b-mrna_bdnf_1-1 3339128 N/A 3339131 predetermined predetermined participant predetermined regulator regulator modulator regulator neutral component neutral component over-expressed over expressed level over-expressed living cell living cell engineered engineered synthetic construct synthetic construct N/A 3339130 regulator target regulator target regulator target modulator target neutral component neutral component over-expressed over expressed level over-expressed living cell living cell engineered engineered tag luciferase tag luciferase tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false r.1470_1477delinstgagcaag mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig 2b, Fig 4d mir_191-mrna_bdnf_1-1 3339128 N/A 3339132 predetermined predetermined participant predetermined regulator regulator modulator regulator neutral component neutral component over-expressed over expressed level over-expressed living cell living cell engineered engineered synthetic construct synthetic construct N/A 3339130 regulator target regulator target regulator target modulator target neutral component neutral component over-expressed over expressed level over-expressed tag luciferase tag luciferase tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false r.1820_1829delinstagtttcact mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig 2b, Fig 4d Shorter isoforms of BDNF mRNA may lack the regulative region for miR191 mir_155-mrna_bdnf_1-1 3339128 N/A 3339133 predetermined predetermined participant predetermined regulator regulator modulator regulator neutral component neutral component over-expressed over expressed level over-expressed living cell living cell engineered engineered synthetic construct synthetic construct N/A 3339130 regulator target regulator target regulator target modulator target neutral component neutral component over-expressed over expressed level over-expressed tag luciferase tag luciferase tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false r.4179_4180delinsat mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig 2b, Fig 4d Shorter isoforms of BDNF mRNA may lack the binding region for miR155