IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ su-2011-16 in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Su Y., Blake-Palmer KG., Fry AC., Best A., Brown AC., Hiemstra TF., Horita S., Zhou A., Toye AM., Karet FE., Su Y., Blake-Palmer KG., Fry AC., Best A., Brown AC., Hiemstra TF., Horita S., Zhou A., Toye AM., Karet FE. fek1000@cam.ac.uk American Journal of Physiology - Renal Physiology 2011 imex curation Only protein-protein interactions imex curation su-2011-9 yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Su Y., Blake-Palmer KG., Fry AC., Best A., Brown AC., Hiemstra TF., Horita S., Zhou A., Toye AM., Karet FE., Su Y., Blake-Palmer KG., Fry AC., Best A., Brown AC., Hiemstra TF., Horita S., Zhou A., Toye AM., Karet FE. fek1000@cam.ac.uk Pre-transformed human kidney matchmaker cDNA library American Journal of Physiology - Renal Physiology 2011 imex curation Only protein-protein interactions imex curation su-2011-10 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Su Y., Blake-Palmer KG., Fry AC., Best A., Brown AC., Hiemstra TF., Horita S., Zhou A., Toye AM., Karet FE., Su Y., Blake-Palmer KG., Fry AC., Best A., Brown AC., Hiemstra TF., Horita S., Zhou A., Toye AM., Karet FE. purified rabbit GAPDH was used in ELISA experiment, the curator assumed the same protein was used for the pulldown. fek1000@cam.ac.uk American Journal of Physiology - Renal Physiology 2011 imex curation Only protein-protein interactions imex curation su-2011-11 human-liver Homo sapiens liver liver liver anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Su Y., Blake-Palmer KG., Fry AC., Best A., Brown AC., Hiemstra TF., Horita S., Zhou A., Toye AM., Karet FE., Su Y., Blake-Palmer KG., Fry AC., Best A., Brown AC., Hiemstra TF., Horita S., Zhou A., Toye AM., Karet FE. fek1000@cam.ac.uk American Journal of Physiology - Renal Physiology 2011 imex curation Only protein-protein interactions imex curation su-2011-12 yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Su Y., Blake-Palmer KG., Fry AC., Best A., Brown AC., Hiemstra TF., Horita S., Zhou A., Toye AM., Karet FE., Su Y., Blake-Palmer KG., Fry AC., Best A., Brown AC., Hiemstra TF., Horita S., Zhou A., Toye AM., Karet FE. fek1000@cam.ac.uk human kidney cDNA library American Journal of Physiology - Renal Physiology 2011 imex curation Only protein-protein interactions imex curation su-2011-13 human-kidney Human kidney kidney kidney [Renal] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Su Y., Blake-Palmer KG., Fry AC., Best A., Brown AC., Hiemstra TF., Horita S., Zhou A., Toye AM., Karet FE., Su Y., Blake-Palmer KG., Fry AC., Best A., Brown AC., Hiemstra TF., Horita S., Zhou A., Toye AM., Karet FE. We initially confirmed that eAE1 was undetectable in kidney membranes by Western blotting, (Supplementary Fig. S1A; all supplementary material for this article is available online at the journal web site) and also ascertained from the supplier that the AE2 epitope sequence is not present in AE1 (see Coimmunoprecipitation). fek1000@cam.ac.uk American Journal of Physiology - Renal Physiology 2011 imex curation Only protein-protein interactions imex curation su-2011-14 rat-kidney Rat kidney kidney kidney [Renal] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Su Y., Blake-Palmer KG., Fry AC., Best A., Brown AC., Hiemstra TF., Horita S., Zhou A., Toye AM., Karet FE., Su Y., Blake-Palmer KG., Fry AC., Best A., Brown AC., Hiemstra TF., Horita S., Zhou A., Toye AM., Karet FE. fek1000@cam.ac.uk American Journal of Physiology - Renal Physiology 2011 imex curation Only protein-protein interactions imex curation su-2011-15 in vitro In vitro elisa enzyme linked immunosorbent assay ELISA ELISA elisa antibody detection identification by antibody antibody detection Su Y., Blake-Palmer KG., Fry AC., Best A., Brown AC., Hiemstra TF., Horita S., Zhou A., Toye AM., Karet FE., Su Y., Blake-Palmer KG., Fry AC., Best A., Brown AC., Hiemstra TF., Horita S., Zhou A., Toye AM., Karet FE. purified rabbit GAPDH was used in ELISA experiment, the curator assumed the same protein was used for the pulldown. fek1000@cam.ac.uk American Journal of Physiology - Renal Physiology 2011 imex curation Only protein-protein interactions imex curation su-2011-17 canlf-mdck Canis familiaris (dog) kidney epithelial cells Canis lupus familiaris Dog Canis familiaris mdck Canine Cocker Spaniel kidney epithelial cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Su Y., Blake-Palmer KG., Fry AC., Best A., Brown AC., Hiemstra TF., Horita S., Zhou A., Toye AM., Karet FE., Su Y., Blake-Palmer KG., Fry AC., Best A., Brown AC., Hiemstra TF., Horita S., Zhou A., Toye AM., Karet FE. fek1000@cam.ac.uk American Journal of Physiology - Renal Physiology 2011 imex curation Only protein-protein interactions imex curation b3at_human Band 3 anion transport protein SLC4A1 AE1 DI EPB3 Anion exchange protein 1 Solute carrier family 4 member 1 protein protein human Homo sapiens Human MEELQDDYEDMMEENLEQEEYEDPDIPESQMEEPAAHDTEATATDYHTTSHPGTHKVYVELQELVMDEKNQELRWMEAARWVQLEENLGENGAWGRPHLSHLTFWSLLELRRVFTKGTVLLDLQETSLAGVANQLLDRFIFEDQIRPQDREELLRALLLKHSHAGELEALGGVKPAVLTRSGDPSQPLLPQHSSLETQLFCEQGDGGTEGHSPSGILEKIPPDSEATLVLVGRADFLEQPVLGFVRLQEAAELEAVELPVPIRFLFVLLGPEAPHIDYTQLGRAAATLMSERVFRIDAYMAQSRGELLHSLEGFLDCSLVLPPTDAPSEQALLSLVPVQRELLRRRYQSSPAKPDSSFYKGLDLNGGPDDPLQQTGQLFGGLVRDIRRRYPYYLSDITDAFSPQVLAAVIFIYFAALSPAITFGGLLGEKTRNQMGVSELLISTAVQGILFALLGAQPLLVVGFSGPLLVFEEAFFSFCETNGLEYIVGRVWIGFWLILLVVLVVAFEGSFLVRFISRYTQEIFSFLISLIFIYETFSKLIKIFQDHPLQKTYNYNVLMVPKPQGPLPNTALLSLVLMAGTFFFAMMLRKFKNSSYFPGKLRRVIGDFGVPISILIMVLVDFFIQDTYTQKLSVPDGFKVSNSSARGWVIHPLGLRSEFPIWMMFASALPALLVFILIFLESQITTLIVSKPERKMVKGSGFHLDLLLVVGMGGVAALFGMPWLSATTVRSVTHANALTVMGKASTPGAAAQIQEVKEQRISGLLVAVLVGLSILMEPILSRIPLAVLFGIFLYMGVTSLSGIQLFDRILLLFKPPKYHPDVPYVKRVKTWRMHLFTGIQIICLAVLWVVKSTPASLALPFVLILTVPLRRVLLPLIFRNVELQCLDADDAKATFDEEEGRDEYDEVAMPV Defects in SLC4A1 are the cause of elliptocytosis type 4 (EL4) [MIM:109270]. EL4 is a Rhesus-unlinked form of hereditary elliptocytosis, a genetically heterogeneous, autosomal dominant hematologic disorder. It is characterized by variable hemolytic anemia and elliptical or oval red cell shape Defects in SLC4A1 are the cause of autosomal dominant distal renal tubular acidosis (dRTA) [MIM:179800]. This disease is characterized by reduced ability to acidify urine, variable hyperchloremic hypokalemic metabolic acidosis, nephrocalcinosis, and nephrolithiasis Defects in SLC4A1 are the cause of autosomal recessive distal renal tubular acidosis (dRTA) [MIM:611590] Band 3 is the major integral glycoprotein of the erythrocyte membrane. Band 3 has two functional domains. Its integral domain mediates a 1:1 exchange of inorganic anions across the membrane, whereas its cytoplasmic domain provides binding sites for cytoskeletal proteins, glycolytic enzymes, and hemoglobin mint Defects in SLC4A1 are a cause of hereditary spherocytosis (HS) [MIM:109270]. HS is a hematologic disorder leading to chronic hemolytic anemia and characterized by numerous abnormally shaped erythrocytes which are generally spheroidal g3p_rabit Glyceraldehyde-3-phosphate dehydrogenase GAPDH GAPD Peptidyl-cysteine S-nitrosylase GAPDH protein protein rabit Oryctolagus cuniculus (Rabbit) Rabbit MVKVGVNGFGRIGRLVTRAAFNSGKVDVVAINDPFIDLHYMVYMFQYDSTHGKFHGTVKAENGKLVINGKAITIFQERDPANIKWGDAGAEYVVESTGVFTTMEKAGAHLKGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIVSNASCTTNCLAPLAKVIHDHFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGAAQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTPNVSVVDLTCRLEKAAKYDDIKKVVKQASEGPLKGILGYTEDQVVSCDFNSATHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMVHMASKE g3p_human Glyceraldehyde-3-phosphate dehydrogenase GAPDH OK/SW-cl.12 CDABP0047 GAPD Peptidyl-cysteine S-nitrosylase GAPDH protein protein human Homo sapiens Human MGKVKVGVNGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLNYMVYMFQYDSTHGKFHGTVKAENGKLVINGNPITIFQERDPSKIKWGDAGAEYVVESTGVFTTMEKAGAHLQGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIISNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGALQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTANVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEHQVVSSDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMAHMASKE b3a2_human Anion exchange protein 2 SLC4A2 AE2 EPB3L1 HKB3 MPB3L Non-erythroid band 3-like protein Solute carrier family 4 member 2 protein protein human Homo sapiens Human MSSAPRRPAKGADSFCTPEPESLGPGTPGFPEQEEDELHRTLGVERFEEILQEAGSRGGEEPGRSYGEEDFEYHRQSSHHIHHPLSTHLPPDARRRKTPQGPGRKPRRRPGASPTGETPTIEEGEEDEDEASEAEGARALTQPSPVSTPSSVQFFLQEDDSADRKAERTSPSSPAPLPHQEATPRASKGAQAGTQVEEAEAEAVAVASGTAGGDDGGASGRPLPKAQPGHRSYNLQERRRIGSMTGAEQALLPRVPTDEIEAQTLATADLDLMKSHRFEDVPGVRRHLVRKNAKGSTQSGREGREPGPTPRARPRAPHKPHEVFVELNELLLDKNQEPQWRETARWIKFEEDVEEETERWGKPHVASLSFRSLLELRRTLAHGAVLLDLDQQTLPGVAHQVVEQMVISDQIKAEDRANVLRALLLKHSHPSDEKDFSFPRNISAGSLGSLLGHHHGQGAESDPHVTEPLMGGVPETRLEVERERELPPPAPPAGITRSKSKHELKLLEKIPENAEATVVLVGCVEFLSRPTMAFVRLREAVELDAVLEVPVPVRFLFLLLGPSSANMDYHEIGRSISTLMSDKQFHEAAYLADEREDLLTAINAFLDCSVVLPPSEVQGEELLRSVAHFQRQMLKKREEQGRLLPTGAGLEPKSAQDKALLQMVEAAGAAEDDPLRRTGRPFGGLIRDVRRRYPHYLSDFRDALDPQCLAAVIFIYFAALSPAITFGGLLGEKTQDLIGVSELIMSTALQGVVFCLLGAQPLLVIGFSGPLLVFEEAFFSFCSSNHLEYLVGRVWIGFWLVFLALLMVALEGSFLVRFVSRFTQEIFAFLISLIFIYETFYKLVKIFQEHPLHGCSASNSSEVDGGENMTWAGARPTLGPGNRSLAGQSGQGKPRGQPNTALLSLVLMAGTFFIAFFLRKFKNSRFFPGRIRRVIGDFGVPIAILIMVLVDYSIEDTYTQKLSVPSGFSVTAPEKRGWVINPLGEKSPFPVWMMVASLLPAILVFILIFMETQITTLIISKKERMLQKGSGFHLDLLLIVAMGGICALFGLPWLAAATVRSVTHANALTVMSKAVAPGDKPKIQEVKEQRVTGLLVALLVGLSIVIGDLLRQIPLAVLFGIFLYMGVTSLNGIQFYERLHLLLMPPKHHPDVTYVKKVRTLRMHLFTALQLLCLALLWAVMSTAASLAFPFILILTVPLRMVVLTRIFTDREMKCLDANEAEPVFDEREGVDEYNEMPMPV p02730-2 Band 3 anion transport protein SLC4A1 AE1 DI EPB3 Anion exchange protein 1 Solute carrier family 4 member 1 Kidney kAE1 protein protein human Homo sapiens Human MDEKNQELRWMEAARWVQLEENLGENGAWGRPHLSHLTFWSLLELRRVFTKGTVLLDLQETSLAGVANQLLDRFIFEDQIRPQDREELLRALLLKHSHAGELEALGGVKPAVLTRSGDPSQPLLPQHSSLETQLFCEQGDGGTEGHSPSGILEKIPPDSEATLVLVGRADFLEQPVLGFVRLQEAAELEAVELPVPIRFLFVLLGPEAPHIDYTQLGRAAATLMSERVFRIDAYMAQSRGELLHSLEGFLDCSLVLPPTDAPSEQALLSLVPVQRELLRRRYQSSPAKPDSSFYKGLDLNGGPDDPLQQTGQLFGGLVRDIRRRYPYYLSDITDAFSPQVLAAVIFIYFAALSPAITFGGLLGEKTRNQMGVSELLISTAVQGILFALLGAQPLLVVGFSGPLLVFEEAFFSFCETNGLEYIVGRVWIGFWLILLVVLVVAFEGSFLVRFISRYTQEIFSFLISLIFIYETFSKLIKIFQDHPLQKTYNYNVLMVPKPQGPLPNTALLSLVLMAGTFFFAMMLRKFKNSSYFPGKLRRVIGDFGVPISILIMVLVDFFIQDTYTQKLSVPDGFKVSNSSARGWVIHPLGLRSEFPIWMMFASALPALLVFILIFLESQITTLIVSKPERKMVKGSGFHLDLLLVVGMGGVAALFGMPWLSATTVRSVTHANALTVMGKASTPGAAAQIQEVKEQRISGLLVAVLVGLSILMEPILSRIPLAVLFGIFLYMGVTSLSGIQLFDRILLLFKPPKYHPDVPYVKRVKTWRMHLFTGIQIICLAVLWVVKSTPASLALPFVLILTVPLRRVLLPLIFRNVELQCLDADDAKATFDEEEGRDEYDEVAMPV b3at_rat Band 3 anion transport protein Anion exchange protein 1 Solute carrier family 4 member 1 Slc4a1 Ae1 protein protein rat Rattus norvegicus (Rat) Rat MGDMQDHEKVLEIPDRDSEEELEHVIEQIAYRDLDIPVTEMQESEALPTEQTATDYIPTSTSTSHPSSSQVYVELQELMMDQRNQELQWVEAAHWIGLEENLREDGVWGRPHLSYLTFWSLLELQKVFSKGTFLLDLAETSLAGVANKLLDSFIYEDQIRPQDRDELLRALLLKRSHAEDLKDLEGVKPAVLTRSGAPSEPLLPHQPSLETKLYCAQAEGGSEEPSPSGILKIPPNSETTLVLVGRASFLVKPVLGFVRLKEAVPLEDLVLPEPVSFLLVLLGPEAPHIDYTQLGRAAATLMTERVFRVTASLAQSRGELLSSLDSFLDCSLVLPPTEAPSEKALLNLVPVQKELLRKRYLPRPAKPDPNLYEALDGGKEGPGDEDDPLRRTGRIFGGLIRDIRRRYPYYLSDITDALSPQVLAAVIFIYFAALSPAVTFGGLLGEKTRNLMGVSELLISTAVQGILFALLGAQPLLVLGFSGPLLVFEEAFYSFCESNNLEYIVGRAWIGFWLILLVVLVVAFEGSFLVQYISRYTQEIFSFLISLIFIYETFSKLIKIFQDYPLQESYAPVVMKPKPQGPVPNTALLSLVLMVGTFLLAMMLRKFKNSTYFPGKLRRVIGDFGVPISILIMVLVDTFIKNTYTQKLSVPDGLKVSNSSARGWVIHPLGLYNHFPKWMMFASVLPALLVFILIFLESQITTLIVSKPERKMIKGSGFHLDLLLVVGMGGVAALFGMPWLSATTVRSVTHANALTVMGKASGPGAAAQIQEVKEQRISGLLVSVLVGLSILMEPILSRIPLAVLFGIFLYMGITSLSGIQLFDRILLLFKPPKYHPDVPFVKRVKTWRMHLFTGIQIICLAVLWVVKSTPASLALPFVLILTVPLRRLLLPLIFRELELQCLDGDDAKVTFDEAEGLDEYDEVPMPV g3p_rat Glyceraldehyde-3-phosphate dehydrogenase Gapdh 38 kDa BFA-dependent ADP-ribosylation substrate BARS-38 Gapd Peptidyl-cysteine S-nitrosylase GAPDH protein protein rat Rattus norvegicus (Rat) Rat MVKVGVNGFGRIGRLVTRAAFSCDKVDIVAINDPFIDLNYMVYMFQYDSTHGKFNGTVKAENGKLVINGKPITIFQERDPANIKWGDAGAEYVVESTGVFTTMEKAGAHLKGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIVSNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGAAQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTPNVSVVDLTCRLEKPAKYDDIKKVVKQAAEGPLKGILGYTEDQVVSCDFNSNSHSSTFDAGAGIALNDNFVKLISWYDNEYGYSNRVVDLMAYMASKE g3p_canlf Glyceraldehyde-3-phosphate dehydrogenase GAPDH GAPD Peptidyl-cysteine S-nitrosylase GAPDH protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MVKVGVNGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLNYMVYMFQYDSTHGKFHGTVKAENGKLVINGKSISIFQERDPANIKWGDAGAEYVVESTGVFTTMEKAGAHLKGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIVSNASCTTNCLAPLAKVIHDHFGIVEGLMTTVHAITATQKTVDGPSGKMWRDGRGAAQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTPNVSVVDLTCRLEKAAKYDDIKKVVKQASEGPLKGILGYTEDQVVSCDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMVYMASKE gapdh-slc4a1-1 3488197 N/A 3488206 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) N/A 3488207 unspecified role unspecified role neutral component neutral component purified purified direct interaction direct interaction false false false 3488197 Fig. 2C gapdh-slc4a1-2 3488197 N/A 3488206 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false phosphorylated Tyr optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3488207 unspecified role unspecified role neutral component neutral component purified purified direct interaction direct interaction false false false 3488197 Fig. 4B gadph-ae1-1 3488198 N/A 3488206 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell N/A 3488208 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell physical association physical association false false false na Parallel yeast two-hybrid assays using AE1C-WT or AE1C-delta11 as bait to screen a human kidney cDNA library yielded 2 clones of the 40 sequenced, which were both 100% identical to the full coding sequence for the human liver-type glycolytic enzyme GAPDH, with no other matches. slc4a1-gapdh-3 3488199 N/A 3488206 unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false P02730:p.Asp902_Glu903delinsAlaAla AA1 mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false P02730:p.Asp905_Glu906delinsAlaAla AA2 mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false P02730:p.Tyr904Ala YA mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3488207 unspecified role unspecified role prey prey purified purified direct interaction direct interaction false false false Fig. 2A, Fig.3 ae2-gapdh-1 3488200 N/A 3488209 unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3488208 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false Fig. 1B, Fig. S1C slc4a1-gapdh-1 3488201 N/A 3488206 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell c-terminal_region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false N/A 3488208 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell physical association physical association false false false Fig. 1A Parallel yeast two-hybrid assays using AE1C-WT or AE1C-delta11 as bait to screen a human kidney cDNA library yielded 2 clones of the 40 sequenced, which were both 100% identical to the full coding sequence for the human liver-type glycolytic enzyme GAPDH, with no other matches. slc4a2-gapdh-1 3488202 N/A 3488210 unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3488208 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false Fig. 1B, Fig. S1B slc4a1-gapdh-2 3488203 N/A 3488211 unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3488212 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false Fig. 1B slc4a1-gapdh-4 3488204 N/A 3488206 unspecified role unspecified role prey prey purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 3488207 unspecified role unspecified role bait bait purified purified direct interaction direct interaction false false false Fig. 2B kae1-gapdh-1 3488205 N/A 3488213 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3488210 unspecified role unspecified role bait bait living cell living cell engineered engineered phosphorylated Tyr optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 4A