DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ kanei_ishii-1997-1 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Kanei-Ishii C., Tanikawa J., Nakai A., Morimoto RI., Ishii S. DIP original experiment: DIP-268341X Science (New York, N.Y.) 1997 IMEx Activation of heat shock transcription factor 3 by c-Myb in the absence of cellular stress. Kanei-Ishii C., Tanikawa J., Nakai A., Morimoto RI., Ishii S. Only protein-protein interactions imex curation kanei_ishii-1997-2 mouse Mus musculus Mouse 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Kanei-Ishii C., Tanikawa J., Nakai A., Morimoto RI., Ishii S. DIP original experiment: DIP-268342X Science (New York, N.Y.) 1997 IMEx Activation of heat shock transcription factor 3 by c-Myb in the absence of cellular stress. Kanei-Ishii C., Tanikawa J., Nakai A., Morimoto RI., Ishii S. Only protein-protein interactions imex curation hsf3_chick Heat shock factor protein 3 HSF3 HSF 3C HSTF 3C Heat shock transcription factor 3 protein protein chick Gallus gallus (Chicken) Chicken MREGSALPGAPGAAPVPGFLAKLWALVEDPQSDDVICWSRNGENFCILDEQRFAKELLPKYFKHNNISSFIRQLNMYGFRKVVALENGMITAEKNSVIEFQHPFFKQGNAHLLENIKRKVSAVRTEDLKVCAEDLHKVLSEVQEMREQQNNMDIRLANMKRENKALWKEVAVLRQKHSQQQKLLSKILQFILSLMRGNYIVGVKRKRSLTDAAGASPSKYSRQYVRIPVESGQAMAFSEHNSDDEDGNRTGLIIRDITDTLENATNGLLAVAHTSGRDRETQTALDPGLPICQVSQPNELSCAEPIPPVHINDVSKPNEMGNVAVELHTAQANAPEDPVSVIDSILNENNSGNQNDPLLDREEIQDFLNCIDASLEELQAMLSGKQYSFGSEAFSDVFNPELPALDMNLMETSPGMENIANMEDSTEDLGASERETAGSKGGQEGTESCDSSVLFQNCVLKWNFSSL myb_mouse Transcriptional activator Myb Myb Proto-oncogene c-Myb protein protein mouse Mus musculus Mouse MARRPRHSIYSSDEDDEDIEMCDHDYDGLLPKSGKRHLGKTRWTREEDEKLKKLVEQNGTDDWKVIANYLPNRTDVQCQHRWQKVLNPELIKGPWTKEEDQRVIELVQKYGPKRWSVIAKHLKGRIGKQCRERWHNHLNPEVKKTSWTEEEDRIIYQAHKRLGNRWAEIAKLLPGRTDNAIKNHWNSTMRRKVEQEGYLQEPSKASQTPVATSFQKNNHLMGFGHASPPSQLSPSGQSSVNSEYPYYHIAEAQNISSHVPYPVALHVNIVNVPQPAAAAIQRHYNDEDPEKEKRIKELELLLMSTENELKGQQALPTQNHTCSYPGWHSTSIVDQTRPHGDSAPVSCLGEHHATPSLPADPGSLPEESASPARCMIVHQGTILDNVKNLLEFAETLQFIDSFLNTSSNHESSGLDAPTLPSTPLIGHKLTPCRDQTVKTQKENSIFRTPAIKRSILESSPRTPTPFKHALAAQEIKYGPLKMLPQTPSHAVEDLQDVIKQESDESGIVAEFQESGPPLLKKIKQEVESPTEKSGNFFCSNHWAENSLSTQLFSQASPVADAPNILTSSVLMTPVSEDEDNVLKAFTVPKNRPLVGPLQPCSGAWEPASCGKTEDQMTASGPARKYVNAFSARTLVM hsf3-myb-1 3293269 N/A 3293271 unspecified role unspecified role bait bait GST Tag GST Tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range false 1-124 binding region 1-124 binding region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3293272 unspecified role unspecified role prey prey 87-193 binding region 87-193 binding region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false hsf3-myb-2 3293270 N/A 3293271 unspecified role unspecified role bait bait mouse Mus musculus Mouse N/A 3293272 unspecified role unspecified role prey prey mouse Mus musculus Mouse physical association physical association false false false