IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ chapel-2017-1 in vitro In vitro affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom antibody detection identification by antibody antibody detection Chapel A., Garcia-Beltran WF., Hölzemer A., Ziegler M., Lunemann S., Martrus G., Altfeld M. LABScreen single-Ag bead sets (One Lambda) were used: Various HLA Class 1 molecules were bound to beads and incubated with KIR2-Fc protein. Binding was detected by fluorescence emitted by a Phycoerythrin conjugated anti-Fc antibody. Both KIR2DS1*002-Fc and KIR2DL1*001- Fc are stated to have been obtained from R+D systems. However this company do not currently sell any KIR2DS1 proteins. The sequence of allele KIR2DS1*002 corresponds to UniProt entry Q14954 (allele KIR2DS1*001) with a R91K mutation -corresponding to variant dbSNP:rs687485. The sequence of allele KIR2DL1*001 corresponds to the entry P43626. marcus.altfeld@hpi.uni-hamburg.de Scientific reports 2017 imex curation Only protein-protein interactions imex curation chapel-2017-2 in vitro In vitro facs fluorescence-activated cell sorting FACS Flow cytometry facs antibody detection identification by antibody antibody detection Chapel A., Garcia-Beltran WF., Hölzemer A., Ziegler M., Lunemann S., Martrus G., Altfeld M. The sequence of allele KIR2DS1*002 corresponds to UniProt entry Q14954 (allele KIR2DS1*001) with a R91K mutation -corresponding to variant dbSNP:rs687485. The sequence of allele KIR2DL1*001 corresponds to the entry P43626. marcus.altfeld@hpi.uni-hamburg.de Scientific reports 2017 imex curation Only protein-protein interactions imex curation 1c02_human HLA class I histocompatibility antigen, Cw-2 alpha chain HLA-C HLAC MHC class I antigen Cw*2 protein protein human Homo sapiens Human MRVMEPRTLILLLSGALALTETWACSHSMRYFYTAVSRPSRGEPHFIAVGYVDDTQFVRFDSDAASPRGEPRGRWVEQEGPEYWDRETQKYNRQAQTDRVNLRKLRGYYNQSEAGSHTLQRMYGCDLGPDGRLLRGYDQSAYDGKDYIALNEDLRSWTAADTAAQITQRKWEAAREAEEWRAYLEGECVEWLRRYLENGKEKLQRAEHPKTHVTHHPVSDHEATLRCWALGFYPTEITLTWQRDGEDQTQDTELVETRPAGDGTFQKWAAVVVPSGEEQRYTCHVQHEGLPEPLTLRWEPSSQPTIPIVGIVAGLAVLAVLAVLGAVVAVVMCRRKSSGGKGGSCSQAASSNSAQGSDESLIASKA The protein could not be merged with EBI-1051396 because of some conflicts with the protein sequence (features which cannot be shifted). ki2s1_human Killer cell immunoglobulin-like receptor 2DS1 KIR2DS1 CD158H CD158 antigen-like family member H MHC class I NK cell receptor Eb6 ActI protein protein human Homo sapiens Human MSLTVVSMACVGFFLLQGAWPHEGVHRKPSLLAHPGRLVKSEETVILQCWSDVMFEHFLLHREGMFNDTLRLIGEHHDGVSKANFSISRMKQDLAGTYRCYGSVTHSPYQLSAPSDPLDIVIIGLYEKPSLSAQPGPTVLAGENVTLSCSSRSSYDMYHLSREGEAHERRLPAGTKVNGTFQANFPLGPATHGGTYRCFGSFRDSPYEWSKSSDPLLVSVTGNPSNSWPSPTEPSSETGNPRHLHVLIGTSVVKIPFTILLFFLLHRWCSDKKNAAVMDQEPAGNRTVNSEDSDEQDHQEVSYA hlac_human HLA class I histocompatibility antigen, C alpha chain HLA-Cw Human leukocyte antigen C HLAC HLA-C protein protein human Homo sapiens Human MRVMAPRALLLLLSGGLALTETWACSHSMRYFDTAVSRPGRGEPRFISVGYVDDTQFVRFDSDAASPRGEPRAPWVEQEGPEYWDRETQKYKRQAQADRVSLRNLRGYYNQSEDGSHTLQRMSGCDLGPDGRLLRGYDQSAYDGKDYIALNEDLRSWTAADTAAQITQRKLEAARAAEQLRAYLEGTCVEWLRRYLENGKETLQRAEPPKTHVTHHPLSDHEATLRCWALGFYPAEITLTWQRDGEDQTQDTELVETRPAGDGTFQKWAAVVVPSGQEQRYTCHMQHEGLQEPLTLSWEPSSQPTIPIMGIVAGLAVLVVLAVLGAVVTAMMCRRKSSGGKGGSCSQAACSNSAQGSDESLITCKA ki2l1_human Killer cell immunoglobulin-like receptor 2DL1 KIR2DL1 CD158 antigen-like family member A CD158A NKAT1 MHC class I NK cell receptor Natural killer-associated transcript 1 p58 natural killer cell receptor clones CL-42/47.11 p58.1 MHC class-I-specific NK receptor protein protein human Homo sapiens Human MSLLVVSMACVGFFLLQGAWPHEGVHRKPSLLAHPGPLVKSEETVILQCWSDVMFEHFLLHREGMFNDTLRLIGEHHDGVSKANFSISRMTQDLAGTYRCYGSVTHSPYQVSAPSDPLDIVIIGLYEKPSLSAQPGPTVLAGENVTLSCSSRSSYDMYHLSREGEAHERRLPAGPKVNGTFQADFPLGPATHGGTYRCFGSFHDSPYEWSKSSDPLLVSVTGNPSNSWPSPTEPSSKTGNPRHLHILIGTSVVIILFILLFFLLHRWCSNKKNAAVMDQESAGNRTANSEDSDEQDPQEVTYTQLNHCVFTQRKITRPSQRPKTPPTDIIVYTELPNAESRSKVVSCP mint 1c01_human HLA class I histocompatibility antigen, Cw-1 alpha chain HLA-C HLAC MHC class I antigen Cw*1 protein protein human Homo sapiens Human MRVMEPRTLILLLSGALALTETWACSHSMKYFFTSVSRPGRGEPRFISVGYVDDTQFVRFDSDAASPRGEPRAPWVEQEGPEYWDRETQKYNRQAQTDRVSLRNLRGYYNQSEAGSHTLQWMCGCDLGPDGRLLRGYDQYAYDGKDYIALNEDLRSWTAADTAAQITQRKWEAAREAEERRAYLEGTCVEWLRRYLENGKESLQRAEHPKTHVTHHPVSDHEATLRCWALGFYPAEITLTWQWDGEDQTQDTELVETRPAGDGTFQKWAAVMVPSGEEQRYTCHVQHEGLPEPLTLRWEPSSQPTIPIVGIVAGLAVLAVLAVLGAVVAVVMCRRKSSGGKGGSCSQAASSNSAQGSDESLIASKA The protein could not be merged with EBI-1051396 because of some conflicts with the protein sequence (features which cannot be shifted). 1c03_human HLA class I histocompatibility antigen, Cw-3 alpha chain HLA-C HLAC MHC class I antigen Cw*3 protein protein human Homo sapiens Human MRVMAPRTLILLLSGALALTETWAGSHSMRYFYTAVSRPGRGEPHFIAVGYVDDTQFVRFDSDAASPRGEPRAPWVEQEGPEYWDRETQKYKRQAQTDRVSLRNLRGYYNQSEAGSHIIQRMYGCDVGPDGRLLRGYDQYAYDGKDYIALNEDLRSWTAADTAAQITQRKWEAAREAEQLRAYLEGLCVEWLRRYLKNGKETLQRAEHPKTHVTHHPVSDHEATLRCWALGFYPAEITLTWQWDGEDQTQDTELVETRPAGDGTFQKWAAVVVPSGEEQRYTCHVQHEGLPEPLTLRWEPSSQPTIPIVGIVAGLAVLAVLAVLGAVVAVVMCRRKSSGGKGGSCSQAASSNSAQGSDESLIACKA The protein could not be merged with EBI-1051396 because of some conflicts with the protein sequence (features which cannot be shifted). hlab_human HLA class I histocompatibility antigen, B alpha chain Human leukocyte antigen B HLA-B HLAB protein protein human Homo sapiens Human MLVMAPRTVLLLLSAALALTETWAGSHSMRYFYTSVSRPGRGEPRFISVGYVDDTQFVRFDSDAASPREEPRAPWIEQEGPEYWDRNTQIYKAQAQTDRESLRNLRGYYNQSEAGSHTLQSMYGCDVGPDGRLLRGHDQYAYDGKDYIALNEDLRSWTAADTAAQITQRKWEAAREAEQRRAYLEGECVEWLRRYLENGKDKLERADPPKTHVTHHPISDHEATLRCWALGFYPAEITLTWQRDGEDQTQDTELVETRPAGDRTFQKWAAVVVPSGEEQRYTCHVQHEGLPKPLTLRWEPSSQSTVPIVGIVAGLAVLAVVVIGAVVAAVMCRRKSSGGKGGSYSQAACSDSAQGSDVSLTA peptide-x peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) SRGPVHHLL b2mg_human Beta-2-microglobulin B2M CDABP0092 HDCMA22P protein protein human Homo sapiens Human MSRSVALAVLALLSLSGLEAIQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM hla_c-kir2ds1-2 3038005 N/A 3038007 unspecified role unspecified role bait bait glu5ala variant variant certain certain sequence position certain certain certain sequence position certain false ile10leu variant variant certain certain sequence position certain certain certain sequence position certain false gly_arg_73-74ala_pro variant variant certain certain sequence position certain certain certain sequence position certain false asn92lys variant variant certain certain sequence position certain certain certain sequence position certain false glu179gln variant variant certain certain sequence position certain certain certain sequence position certain false lys202thr variant variant certain certain sequence position certain certain certain sequence position certain false ser364cys variant variant certain certain sequence position certain certain certain sequence position certain false N/A 3038008 unspecified role unspecified role prey prey ecd sufficient to bind sufficient binding region sufficient to bind range range range range false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false direct interaction direct interaction false false false Fig 1A and text C0202 allele hla_c-kir2ds1-1 3038005 N/A 3038009 unspecified role unspecified role bait bait N/A 3038008 unspecified role unspecified role prey prey ecd sufficient to bind sufficient binding region sufficient to bind range range range range false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false direct interaction direct interaction false false false Fig 1A-2 and text C0401 allele hla_c-kir2ds1-3 3038005 n/a 3038009 unspecified role unspecified role bait bait N/A 3038008 unspecified role unspecified role prey prey ecd sufficient to bind sufficient binding region sufficient to bind range range range range false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false direct interaction direct interaction false false false Fig 1A-3 and text C0501 allele hla_c-kir2ds1-4 3038005 n/a 3038009 unspecified role unspecified role bait bait N/A 3038008 unspecified role unspecified role prey prey ecd sufficient to bind sufficient binding region sufficient to bind range range range range false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false direct interaction direct interaction false false false Fig 1A-4 and text C0602 allele hla_c-kir2ds1-5 3038005 n/a 3038009 unspecified role unspecified role bait bait N/A 3038008 unspecified role unspecified role prey prey ecd sufficient to bind sufficient binding region sufficient to bind range range range range false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false direct interaction direct interaction false false false Fig 1A-5 and text C1502 allele hla_c-kir2ds1-6 3038005 n/a 3038009 unspecified role unspecified role bait bait N/A 3038008 unspecified role unspecified role prey prey ecd sufficient to bind sufficient binding region sufficient to bind range range range range false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false direct interaction direct interaction false false false Fig 1A-6 and text C1701 allele hla_c-kir2ds1-7 3038005 N/A 3038008 unspecified role unspecified role prey prey ecd sufficient to bind sufficient binding region sufficient to bind range range range range false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 3038009 unspecified role unspecified role bait bait val319ala variant variant certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false Fig 1A-7 and text C1802 allele hla_c-kir2dl3-14 3038005 N/A 3038010 unspecified role unspecified role prey prey ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false mouse-nso NSO-mouse myeloma subclone of NS-1 ns0 NS0 Mouse Myeloma subclone of NS-1 http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=85110503&collection=ecacc_gc from ecacc N/A 3038011 unspecified role unspecified role bait bait glu5ala variant variant certain certain sequence position certain certain certain sequence position certain false asn92lys variant variant certain certain sequence position certain certain certain sequence position certain false glu179gln variant variant certain certain sequence position certain certain certain sequence position certain false ser202thr variant variant certain certain sequence position certain certain certain sequence position certain false ser364cys variant variant certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false Fig 1Ai and text C0102 allele hla_c-kir2dl1-19 3038005 N/A 3038010 unspecified role unspecified role prey prey ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false mouse-nso NSO-mouse myeloma subclone of NS-1 ns0 NS0 Mouse Myeloma subclone of NS-1 http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=85110503&collection=ecacc_gc from ecacc N/A 3038012 unspecified role unspecified role bait bait gly115arg variant variant certain certain sequence position certain certain certain sequence position certain false ile119leu,tyr140ser variant variant certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false Fig 1Ai-2 and text C0302, C0303, C0304 (canonical entry) alleles all bind hla_c-kir2dl1-20 3038005 N/A 3038010 unspecified role unspecified role prey prey ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false mouse-nso NSO-mouse myeloma subclone of NS-1 ns0 NS0 Mouse Myeloma subclone of NS-1 http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=85110503&collection=ecacc_gc from ecacc n/a 3038009 unspecified role unspecified role bait bait direct interaction direct interaction false false false Fig 1Ai-3 and text C0702 allele hla_c-kir2dl1-21 3038005 N/A 3038010 unspecified role unspecified role prey prey ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false mouse-nso NSO-mouse myeloma subclone of NS-1 ns0 NS0 Mouse Myeloma subclone of NS-1 http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=85110503&collection=ecacc_gc from ecacc n/a 3038009 unspecified role unspecified role bait bait direct interaction direct interaction false false false Fig 1Ai-4 and text C0801 allele hla_c-kir2dl1-22 3038005 N/A 3038010 unspecified role unspecified role prey prey ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false mouse-nso NSO-mouse myeloma subclone of NS-1 ns0 NS0 Mouse Myeloma subclone of NS-1 http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=85110503&collection=ecacc_gc from ecacc n/a 3038009 unspecified role unspecified role bait bait arg121trp variant variant certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false Fig 1Ai-5 and text C1203 allele hla_c-kir2dl1-23 3038005 N/A 3038010 unspecified role unspecified role prey prey ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false mouse-nso NSO-mouse myeloma subclone of NS-1 ns0 NS0 Mouse Myeloma subclone of NS-1 http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=85110503&collection=ecacc_gc from ecacc n/a 3038009 unspecified role unspecified role bait bait direct interaction direct interaction false false false Fig 1Ai-6 and text C1601 allele hla_c-kir2dl1-24 3038005 N/A 3038010 unspecified role unspecified role prey prey ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false mouse-nso NSO-mouse myeloma subclone of NS-1 ns0 NS0 Mouse Myeloma subclone of NS-1 http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=85110503&collection=ecacc_gc from ecacc n/a 3038013 unspecified role unspecified role bait bait direct interaction direct interaction false false false Fig 1Ai-7 and text B4601 allele hla_b-kir2dl1-1 3038005 N/A 3038010 unspecified role unspecified role prey prey ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false mouse-nso NSO-mouse myeloma subclone of NS-1 ns0 NS0 Mouse Myeloma subclone of NS-1 http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=85110503&collection=ecacc_gc from ecacc n/a 3038013 unspecified role unspecified role bait bait direct interaction direct interaction false false false Fig 1Ai-8 and text B7301 allele (canonical) hla_c-kir2ds1-8 3038006 N/A 3038014 unspecified role unspecified role neutral component neutral component region rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3038015 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 3038009 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3038008 unspecified role unspecified role neutral component neutral component ecd sufficient to bind sufficient binding region sufficient to bind range range range range false cd3_zeta-tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-jurkat Homo sapiens jurkat Human T cell leukemia 3038114 3038112 physical association physical association false false false 3E b2m-hla_c-1 3038006 N/A 3038014 unspecified role unspecified role neutral component neutral component region rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3038015 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 3038009 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3038008 unspecified role unspecified role neutral component neutral component human-nk_cells human-Natural Killer cells Human nk_cells Natural killer cells 3038125 3038123 physical association physical association false false false 5C KIR2DS1(+) KIR2DL1(−) NK cell clones