DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ aydin-2016-1 in vitro In vitro bio-layer interferometry bio-layer interferometry bli bli predetermined predetermined participant predetermined Aydin H., Sultana A., Li S., Thavalingam A., Lee JE. DIP original experiment: DIP-269116X Nature 2016 IMEx Molecular architecture of the human sperm IZUMO1 and egg JUNO fertilization complex. Aydin H., Sultana A., Li S., Thavalingam A., Lee JE. Only protein-protein interactions imex curation aydin-2016-2 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Aydin H., Sultana A., Li S., Thavalingam A., Lee JE. DIP original experiment: DIP-269114X Nature 2016 IMEx Molecular architecture of the human sperm IZUMO1 and egg JUNO fertilization complex. Aydin H., Sultana A., Li S., Thavalingam A., Lee JE. Only protein-protein interactions imex curation aydin-2016-3 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Aydin H., Sultana A., Li S., Thavalingam A., Lee JE. DIP original experiment: DIP-269121X Nature 2016 IMEx Molecular architecture of the human sperm IZUMO1 and egg JUNO fertilization complex. Aydin H., Sultana A., Li S., Thavalingam A., Lee JE. Only protein-protein interactions imex curation aydin-2016-4 in vitro In vitro saxs x ray scattering saxs predetermined predetermined participant predetermined Aydin H., Sultana A., Li S., Thavalingam A., Lee JE. DIP original experiment: DIP-269118X Nature 2016 IMEx Molecular architecture of the human sperm IZUMO1 and egg JUNO fertilization complex. Aydin H., Sultana A., Li S., Thavalingam A., Lee JE. Only protein-protein interactions imex curation aydin-2016-5 in vitro In vitro dls dynamic light scattering dls predetermined predetermined participant predetermined Aydin H., Sultana A., Li S., Thavalingam A., Lee JE. DIP original experiment: DIP-269115X Nature 2016 IMEx Molecular architecture of the human sperm IZUMO1 and egg JUNO fertilization complex. Aydin H., Sultana A., Li S., Thavalingam A., Lee JE. Only protein-protein interactions imex curation aydin-2016-6 in vitro In vitro bio-layer interferometry bio-layer interferometry bli bli predetermined predetermined participant predetermined Aydin H., Sultana A., Li S., Thavalingam A., Lee JE. DIP original experiment: DIP-269119X Nature 2016 IMEx Molecular architecture of the human sperm IZUMO1 and egg JUNO fertilization complex. Aydin H., Sultana A., Li S., Thavalingam A., Lee JE. Only protein-protein interactions imex curation aydin-2016-7 in vitro In vitro h1-h2 ms mass spectrometry study of hydrogen/deuterium exchange h1-h2 ms predetermined predetermined participant predetermined Aydin H., Sultana A., Li S., Thavalingam A., Lee JE. DIP original experiment: DIP-269120X Nature 2016 IMEx Molecular architecture of the human sperm IZUMO1 and egg JUNO fertilization complex. Aydin H., Sultana A., Li S., Thavalingam A., Lee JE. Only protein-protein interactions imex curation aydin-2016-8 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Aydin H., Sultana A., Li S., Thavalingam A., Lee JE. DIP original experiment: DIP-269117X Nature 2016 IMEx Molecular architecture of the human sperm IZUMO1 and egg JUNO fertilization complex. Aydin H., Sultana A., Li S., Thavalingam A., Lee JE. Only protein-protein interactions imex curation a6nd01-1 Sperm-egg fusion protein Juno Folate receptor 4 Folate receptor delta IZUMO1 receptor protein JUNO IZUMO1R FOLR4 JUNO protein protein human Homo sapiens Human MACWWPLLLELWTVMPTWAGDELLNICMNAKHHKRVPSPEDKLYEECIPWKDNACCTLTTSWEAHLDVSPLYNFSLFHCGLLMPGCRKHFIQAICFYECSPNLGPWIQPVGSLGWEVAPSGQGERVVNVPLCQEDCEEWWEDCRMSYTCKSNWRGGWDWSQGKNRCPKGAQCLPFSHYFPTPADLCEKTWSNSFKASPERRNSGRCLQKWFEPAQGNPNVAVARLFASSAPSWELSYTIMVCSLFLPFLS No experimental confirmation available. Gene prediction based on EST data. q8iyv9-1 Izumo sperm-egg fusion protein 1 Oocyte binding/fusion factor Sperm-specific protein izumo IZUMO1 protein protein human Homo sapiens Human MGPHFTLLCAALAGCLLPAEGCVICDPSVVLALKSLEKDYLPGHLDAKHHKAMMERVENAVKDFQELSLNEDAYMGVVDEATLQKGSWSLLKDLKRITDSDVKGDLFVKELFWMLHLQKETFATYVARFQKEAYCPNKCGVMLQTLIWCKNCKKEVHACRKSYDCGERNVEVPQMEDMILDCELNWHQASEGLTDYSFYRVWGNNTETLVSKGKEATLTKPMVGPEDAGSYRCELGSVNSSPATIINFHVTVLPKMIKEEKPSPNIVTPGEATTESSISLQPLQPEKMLASRLLGLLICGSLALITGLTFAIFRRRKVIDFIKSSLFGLGSGAAEQTQVPKEKATDSRQQ izumo1r-izumo1-1 3292190 N/A 3292198 unspecified role unspecified role bait bait Region 20-228 Region 20-228 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3292199 unspecified role unspecified role prey prey Region 22-254 Region 22-254 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q8IYV9-1:p.Trp148Ala W148A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q8IYV9-1:p.Arg160Ala R160A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q8IYV9-1:p.Arg160Glu R160E mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q8IYV9-1:p.His157Ala H157A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q8IYV9-1:p.Glu71Ala E71A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q8IYV9-1:p.Glu71Lys E71K mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction false false false izumo1-izumo1r-6 3292191 N/A 3292198 unspecified role unspecified role unspecified role unspecified role Region 20-228 Region 20-228 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3292199 unspecified role unspecified role unspecified role unspecified role Region 22-254 Region 22-254 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction false false false izumo1-izumo1r-7 3292192 N/A 3292198 unspecified role unspecified role unspecified role unspecified role Region 20-228 Region 20-228 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3292199 unspecified role unspecified role unspecified role unspecified role Region 22-254 Region 22-254 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction false false false izumo1-izumo1r-8 3292193 N/A 3292198 unspecified role unspecified role unspecified role unspecified role Region 20-228 Region 20-228 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3292199 unspecified role unspecified role unspecified role unspecified role Region 22-254 Region 22-254 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction false false false izumo1-izumo1r-9 3292194 N/A 3292198 unspecified role unspecified role unspecified role unspecified role Region 20-228 Region 20-228 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3292199 unspecified role unspecified role unspecified role unspecified role Region 22-254 Region 22-254 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction false false false izumo1r-izumo1-2 3292195 N/A 3292198 unspecified role unspecified role bait bait Region 20-228 Region 20-228 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false A6ND01-1:p.Lys163Glu K163E mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false A6ND01-1:p.Lys163Ala K163A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false A6ND01-1:p.Leu81Ala L81A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false A6ND01-1:p.Trp62Ala W62A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false A6ND01-1:p.Glu45Ala E45A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false A6ND01-1:p.Glu45Lys E45K mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3292199 unspecified role unspecified role prey prey Region 22-254 Region 22-254 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction false false false izumo1-izumo1r-10 3292196 N/A 3292198 unspecified role unspecified role unspecified role unspecified role Region 20-228 Region 20-228 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3292199 unspecified role unspecified role unspecified role unspecified role Region 22-254 Region 22-254 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction false false false izumo1r-izumo1-3 3292197 N/A 3292198 unspecified role unspecified role bait bait Region 20-228 Region 20-228 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3292199 unspecified role unspecified role prey prey Region 22-254 Region 22-254 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction false false false