DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ ulrich-2016-1 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Ulrich AK., Schulz JF., Kamprad A., Schütze T., Wahl MC. DIP original experiment: DIP-268517X Structure (London, England : 1993) 2016 IMEx Structural Basis for the Functional Coupling of the Alternative Splicing Factors Smu1 and RED. Ulrich AK., Schulz JF., Kamprad A., Schütze T., Wahl MC. Only protein-protein interactions imex curation ulrich-2016-2 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Ulrich AK., Schulz JF., Kamprad A., Schütze T., Wahl MC. DIP original experiment: DIP-268515X Structure (London, England : 1993) 2016 IMEx Structural Basis for the Functional Coupling of the Alternative Splicing Factors Smu1 and RED. Ulrich AK., Schulz JF., Kamprad A., Schütze T., Wahl MC. Only protein-protein interactions imex curation ulrich-2016-3 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Ulrich AK., Schulz JF., Kamprad A., Schütze T., Wahl MC. DIP original experiment: DIP-268520X Structure (London, England : 1993) 2016 IMEx Structural Basis for the Functional Coupling of the Alternative Splicing Factors Smu1 and RED. Ulrich AK., Schulz JF., Kamprad A., Schütze T., Wahl MC. Only protein-protein interactions imex curation ulrich-2016-4 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Ulrich AK., Schulz JF., Kamprad A., Schütze T., Wahl MC. DIP original experiment: DIP-268518X Structure (London, England : 1993) 2016 IMEx Structural Basis for the Functional Coupling of the Alternative Splicing Factors Smu1 and RED. Ulrich AK., Schulz JF., Kamprad A., Schütze T., Wahl MC. Only protein-protein interactions imex curation ulrich-2016-5 ecolx Escherichia coli light scattering light scattering predetermined predetermined participant predetermined Ulrich AK., Schulz JF., Kamprad A., Schütze T., Wahl MC. DIP original experiment: DIP-268516X Structure (London, England : 1993) 2016 IMEx Structural Basis for the Functional Coupling of the Alternative Splicing Factors Smu1 and RED. Ulrich AK., Schulz JF., Kamprad A., Schütze T., Wahl MC. Only protein-protein interactions imex curation ulrich-2016-6 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Ulrich AK., Schulz JF., Kamprad A., Schütze T., Wahl MC. DIP original experiment: DIP-268521X Structure (London, England : 1993) 2016 IMEx Structural Basis for the Functional Coupling of the Alternative Splicing Factors Smu1 and RED. Ulrich AK., Schulz JF., Kamprad A., Schütze T., Wahl MC. Only protein-protein interactions imex curation ulrich-2016-7 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Ulrich AK., Schulz JF., Kamprad A., Schütze T., Wahl MC. DIP original experiment: DIP-268519X Structure (London, England : 1993) 2016 IMEx Structural Basis for the Functional Coupling of the Alternative Splicing Factors Smu1 and RED. Ulrich AK., Schulz JF., Kamprad A., Schütze T., Wahl MC. Only protein-protein interactions imex curation smu1_caeel Smu-1 suppressor of mec-8 and unc-52 protein smu-1 Suppressor of Mec and Unc defects 1 CC4.3 protein protein caeel Caenorhabditis elegans MSSIEIESSDVIRLIEQFLKESNLHRTLAILQEETNVSLNTVDSIDGFCNEITSGNWDNVLKTVQSLKLPAKKLIDLYEHVIIELVELRELATARLVARQTDPMILLKQIDPDRFARLESLINRPYFDGQEVYGDVSKEKRRSVIAQTLSSEVHVVAPSRLLSLLGQSLKWQLHQGLLPPGTAIDLFRGKAAQKEQIEERYPTMMARSIKFSTKSYPESAVFSPDANYLVSGSKDGFIEVWNYMNGKLRKDLKYQAQDNLMMMDAAVRCISFSRDSEMLATGSIDGKIKVWKVETGDCLRRFDRAHTKGVCAVRFSKDNSHILSGGNDHVVRVHGMKSGKCLKEMRGHSSYITDVRYSDEGNHIISCSTDGSIRVWHGKSGECLSTFRVGSEDYPILNVIPIPKSDPPQMIVCNRSNTLYVVNISGQVVRTMTSGKREKGDFINCILSPKGEWAYAIAEDGVMYCFMVLSGTLETTLPVTERLPIGLAHHPHQNLIASYAEDGLLKLWTD smu2_caeel Smu-2 suppressor of mec-8 and unc-52 protein smu-2 RED homolog Suppressor of Mec and Unc defects 2 Y49F6B.4 protein protein caeel Caenorhabditis elegans MADNPTNLRNADFRKLLTSARSDRPAVSAFAKPADPKTGDDKPASFKHKHLKPAKFKKPQAAAHGKAKKEKTEADEDEAALKNILKNYRDRAAERRKQGDEKEDPSKLTAAYRAVPGDARSAQDQADLRKQAILESKYLGGDLEHTHLVKGLDYSLLNKVRSEIDKSDDDDDDDIDTAFDEKVTSSSSSSKPSEASLLAQELAQSHSENRMVRSLHRVLFKNEVPLHNQLFAKGRMAYVVELEDEETDIPTTLLRSLHDLPRAESAQSIQANNLIILKLSHVLSHLRAEPKKKKKEEFRVQLGSRDAPGAAAAAPGAKGDSIYDDLDDYVPSRKSRDSRDAGRRGSRRDRSRDRSRDRDRDRDRDNRDRYFEKSANSRREEEQNRREQQRERERAEQERRREREKEREQEKAKEREKKRKELEESSGYDECYPGGLVEMGGAWDSDEEADYSKMDAGPKKNQAVNRWDFDTEEEYASYMEGREALPKAAYQYGVKNGEGGRKNKKQSAVSDAKRLDRELNEINKIMDKRKAGGDGAGGGGDYKKPKY smu_1-1 3291580 N/A 3291587 unspecified role unspecified role unspecified role unspecified role Region 1-181 Region 1-181 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false smu_1-2 3291581 N/A 3291587 unspecified role unspecified role unspecified role unspecified role Region 1-181 Region 1-181 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false G5EEG7:p.Ile15Ala I15A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false G5EEG7:p.Ile15Arg I15R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false G5EEG7:p.Phe18Ser F18S/R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false G5EEG7:p.Phe18Arg mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false smu_2-smu_1-2 3291582 N/A 3291588 unspecified role unspecified role bait bait Region 163-223 Region 163-223 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3291587 unspecified role unspecified role prey prey Region 1-181 Region 1-181 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false smu_1-smu_2-1 3291583 N/A 3291588 unspecified role unspecified role unspecified role unspecified role Region 163-223 Region 163-223 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3291587 unspecified role unspecified role unspecified role unspecified role Region 1-181 Region 1-181 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false G5EEG7:p.Leu96Arg L96R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false smu_1-3 3291584 N/A 3291587 unspecified role unspecified role unspecified role unspecified role Region 1-181 Region 1-181 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false G5EEG7:p.Ile15Ala I15A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false G5EEG7:p.Ile15Arg I15R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false G5EEG7:p.Phe18Ser F18S/R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false G5EEG7:p.Phe18Arg mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false smu_1-smu_2-2 3291585 N/A 3291588 unspecified role unspecified role unspecified role unspecified role Region 163-223 Region 163-223 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q9N4U5:p.Phe220Arg F220R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q9N4U5:p.Val212Arg V212R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3291587 unspecified role unspecified role unspecified role unspecified role Region 1-181 Region 1-181 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false smu_1-smu_2-3 3291586 N/A 3291588 unspecified role unspecified role unspecified role unspecified role Region 163-223 Region 163-223 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3291587 unspecified role unspecified role unspecified role unspecified role Region 1-181 Region 1-181 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false