DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ van-2016-1 in vitro In vitro methyltransferase as methyltransferase assay methyltransferase as predetermined predetermined participant predetermined Van Aller GS., Graves AP., Elkins PA., Bonnette WG., McDevitt PJ., Zappacosta F., Annan RS., Dean TW., Su DS., Carpenter CL., Mohammad HP., Kruger RG. DIP original experiment: DIP-268507X Structure (London, England : 1993) 2016 IMEx Structure-Based Design of a Novel SMYD3 Inhibitor that Bridges the SAM-and MEKK2-Binding Pockets. Van Aller GS., Graves AP., Elkins PA., Bonnette WG., McDevitt PJ., Zappacosta F., Annan RS., Dean TW., Su DS., Carpenter CL., Mohammad HP., Kruger RG. Only protein-protein interactions imex curation van-2016-2 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Van Aller GS., Graves AP., Elkins PA., Bonnette WG., McDevitt PJ., Zappacosta F., Annan RS., Dean TW., Su DS., Carpenter CL., Mohammad HP., Kruger RG. DIP original experiment: DIP-268505X Structure (London, England : 1993) 2016 IMEx Structure-Based Design of a Novel SMYD3 Inhibitor that Bridges the SAM-and MEKK2-Binding Pockets. Van Aller GS., Graves AP., Elkins PA., Bonnette WG., McDevitt PJ., Zappacosta F., Annan RS., Dean TW., Su DS., Carpenter CL., Mohammad HP., Kruger RG. Only protein-protein interactions imex curation van-2016-3 in vitro In vitro methyltransferase as methyltransferase assay methyltransferase as predetermined predetermined participant predetermined Van Aller GS., Graves AP., Elkins PA., Bonnette WG., McDevitt PJ., Zappacosta F., Annan RS., Dean TW., Su DS., Carpenter CL., Mohammad HP., Kruger RG. DIP original experiment: DIP-268506X Structure (London, England : 1993) 2016 IMEx Structure-Based Design of a Novel SMYD3 Inhibitor that Bridges the SAM-and MEKK2-Binding Pockets. Van Aller GS., Graves AP., Elkins PA., Bonnette WG., McDevitt PJ., Zappacosta F., Annan RS., Dean TW., Su DS., Carpenter CL., Mohammad HP., Kruger RG. Only protein-protein interactions imex curation m3k2_human Mitogen-activated protein kinase kinase kinase 2 MAP3K2 MAPKKK2 MEKK2 MAPK/ERK kinase kinase 2 protein protein human Homo sapiens Human MDDQQALNSIMQDLAVLHKASRPALSLQETRKAKSSSPKKQNDVRVKFEHRGEKRILQFPRPVKLEDLRSKAKIAFGQSMDLHYTNNELVIPLTTQDDLDKAVELLDRSIHMKSLKILLVINGSTQATNLEPLPSLEDLDNTVFGAERKKRLSIIGPTSRDRSSPPPGYIPDELHQVARNGSFTSINSEGEFIPESMDQMLDPLSLSSPENSGSGSCPSLDSPLDGESYPKSRMPRAQSYPDNHQEFSDYDNPIFEKFGKGGTYPRRYHVSYHHQEYNDGRKTFPRARRTQGTSLRSPVSFSPTDHSLSTSSGSSIFTPEYDDSRIRRRGSDIDNPTLTVMDISPPSRSPRAPTNWRLGKLLGQGAFGRVYLCYDVDTGRELAVKQVQFDPDSPETSKEVNALECEIQLLKNLLHERIVQYYGCLRDPQEKTLSIFMEYMPGGSIKDQLKAYGALTENVTRKYTRQILEGVHYLHSNMIVHRDIKGANILRDSTGNVKLGDFGASKRLQTICLSGTGMKSVTGTPYWMSPEVISGEGYGRKADIWSVACTVVEMLTEKPPWAEFEAMAAIFKIATQPTNPKLPPHVSDYTRDFLKRIFVEAKLRPSADELLRHMFVHYH q9h7b4-1 Histone-lysine N-methyltransferase SMYD3 SMYD3 ZMYND1 ZNFN3A1 SET and MYND domain-containing protein 3 Zinc finger MYND domain-containing protein 1 protein protein human Homo sapiens Human MEPLKVEKFATAKRGNGLRAVTPLRPGELLFRSDPLAYTVCKGSRGVVCDRCLLGKEKLMRCSQCRVAKYCSAKCQKKAWPDHKRECKCLKSCKPRYPPDSVRLLGRVVFKLMDGAPSESEKLYSFYDLESNINKLTEDKKEGLRQLVMTFQHFMREEIQDASQLPPAFDLFEAFAKVICNSFTICNAEMQEVGVGLYPSISLLNHSCDPNCSIVFNGPHLLLRAVRDIEVGEELTICYLDMLMTSEERRKQLRDQYCFECDCFRCQTQDKDADMLTGDEQVWKEVQESLKKIEELKAHWKWEQVLAMCQAIISSNSERLPDINIYQLKVLDCAMDACINLGLLEEALFYGTRTMEPYRIFFPGSHPVRGVQVMKVGKLQLHQGMFPQAMKNLRLAFDIMRVTHGREHSLIEDLILLLEECDANIRAS map3k2-smyd3-1 3291278 N/A 3291281 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 249-273 Region 249-273 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3291282 enzyme enzyme unspecified role unspecified role uniba unidentified baculovirus methylation methylation reaction methylation false false false map3k2-smyd3-2 3291279 N/A 3291281 unspecified role unspecified role unspecified role unspecified role Region 250-264 Region 250-264 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3291282 unspecified role unspecified role unspecified role unspecified role uniba unidentified baculovirus direct interaction direct interaction false false false map3k2-smyd3-3 3291280 N/A 3291281 enzyme target enzyme target substrate substrate unspecified role unspecified role K260me1 K260me1 me1lys monomethylated L-lysine Me1Lys Methyl Methylation methylk modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain false Resulting PTM K260me2 K260me2 n6me2lys N6,N6-dimethyl-L-lysine (2S)-2-amino-6-(dimethylamino)hexanoic acid MOD_RES N6,N6-dimethyllysine N(zeta)-dimethyllysine N6,N6-dimethyl-L-lysine N6,N6-dimethylated L-lysine epsilon-dimethyllysine lysine derivative Lys(y) Dimethyl N6Me2Lys di-Methylation dimethylk modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain false Resulting PTM K260me3 K260me3 n6me3+lys N6,N6,N6-trimethyl-L-lysine (5S)-5-amino-5-carboxy-N,N,N-trimethylpentan-1-aminium 2-amino-6-(trimethylammonio)hexanoic acid 5-azanyl-5-carboxy-N,N,N-trimethylpentanazanium MOD_RES N6,N6,N6-trimethyllysine N(zeta)-trimethyllysine N6,N6,N6-trimethyl-L-lysine N6,N6,N6-trimethylated L-lysine N6,N6,N6-trimethyllysin-N6-ium N6,N6,N6-trimethyllysine cation epsilon-trimethyllysine N-trimethylation (of lysine) N6Me3+Lys Trimethyl tri-Methylation modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain false Resulting PTM uniba unidentified baculovirus N/A 3291282 enzyme enzyme unspecified role unspecified role uniba unidentified baculovirus methylation methylation reaction methylation false false false