DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ qiu-2016-1 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip de novo protein sequence de novo protein sequencing by mass spectrometry de novo protein sequence Qiu J., Sheedlo MJ., Yu K., Tan Y., Nakayasu ES., Das C., Liu X., Luo ZQ. DIP original experiment: DIP-268777X Nature 2016 IMEx Ubiquitination independent of E1 and E2 enzymes by bacterial effectors. Qiu J., Sheedlo MJ., Yu K., Tan Y., Nakayasu ES., Das C., Liu X., Luo ZQ. Only protein-protein interactions imex curation qiu-2016-2 in vitro In vitro comigration in sds comigration in sds page comigration in sds predetermined predetermined participant predetermined Qiu J., Sheedlo MJ., Yu K., Tan Y., Nakayasu ES., Das C., Liu X., Luo ZQ. DIP original experiment: DIP-268780X Nature 2016 IMEx Ubiquitination independent of E1 and E2 enzymes by bacterial effectors. Qiu J., Sheedlo MJ., Yu K., Tan Y., Nakayasu ES., Das C., Liu X., Luo ZQ. Only protein-protein interactions imex curation qiu-2016-3 in vitro In vitro comigration in sds comigration in sds page comigration in sds western blot western blot Immuno blot Immuno blot Qiu J., Sheedlo MJ., Yu K., Tan Y., Nakayasu ES., Das C., Liu X., Luo ZQ. DIP original experiment: DIP-268778X Nature 2016 IMEx Ubiquitination independent of E1 and E2 enzymes by bacterial effectors. Qiu J., Sheedlo MJ., Yu K., Tan Y., Nakayasu ES., Das C., Liu X., Luo ZQ. Only protein-protein interactions imex curation qiu-2016-4 in vitro In vitro comigration in sds comigration in sds page comigration in sds predetermined predetermined participant predetermined Qiu J., Sheedlo MJ., Yu K., Tan Y., Nakayasu ES., Das C., Liu X., Luo ZQ. DIP original experiment: DIP-268781X Nature 2016 IMEx Ubiquitination independent of E1 and E2 enzymes by bacterial effectors. Qiu J., Sheedlo MJ., Yu K., Tan Y., Nakayasu ES., Das C., Liu X., Luo ZQ. Only protein-protein interactions imex curation qiu-2016-5 in vitro In vitro comigration in sds comigration in sds page comigration in sds western blot western blot Immuno blot Immuno blot Qiu J., Sheedlo MJ., Yu K., Tan Y., Nakayasu ES., Das C., Liu X., Luo ZQ. DIP original experiment: DIP-268779X Nature 2016 IMEx Ubiquitination independent of E1 and E2 enzymes by bacterial effectors. Qiu J., Sheedlo MJ., Yu K., Tan Y., Nakayasu ES., Das C., Liu X., Luo ZQ. Only protein-protein interactions imex curation ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG rb33b_human Ras-related protein Rab-33B RAB33B protein protein human Homo sapiens Human MAEEMESSLEASFSSSGAVSGASGFLPPARSRIFKIIVIGDSNVGKTCLTYRFCAGRFPDRTEATIGVDFRERAVEIDGERIKIQLWDTAGQERFRKSMVQHYYRNVHAVVFVYDMTNMASFHSLPSWIEECKQHLLANDIPRILVGNKCDLRSAIQVPTDLAQKFADTHSMPLFETSAKNPNDNDHVEAIFMTLAHKLKSHKPLMLSQPPDNGIILKPEPKPAMTCWC q6rcr0_legpn protein protein legpn Legionella pneumophila MGEAIMPKYVEGVELTQEGMHAIFARMGYGDITSGSIYNGVPTIDTGALNRQGFMPVLTGVGPHRDSGHWIMLIKGPGNQYYLFDPLGKTSGEGYQNILAAQLPMGSTLSVIPNGSGLNMGLCGYWVASAGLRAHQALNQHNPPTLLNVGQTITNEMRNELDHDGYRKITGWLRAVADEFPEGDPQLDGKALRENTEKDLKIEIPTLVLPGKDTSPKEMSVKPTAPQDKSVPVWNGFSLYTDDTVKAAAQYAYDNYLGKPYTGSVESAPANFGGRMVYRQHHGLSHTLRTMAYAELIVEEARKAKLRGETLGKFKDGRTIADVTPQELKKIMIAQAFFVAGRDDEASDAKNYQKYHEQSRDAFLKYVKDNESTLIPDVFKDQEDVNFYARVIEDKSHDWESTPAHVLINQGHMVDLVRVKQPPESFLQRYFSSMQRWIGSQATEAVFGIQRQFFHATYEVVAGFDSDNKEPHLVVSGLGRYVIGEDGQPIREAPKKGQKEGDLKVFPQTYKLKENERLMRVDEFLKLPEIQNTFPGSGKHLQGGMPGMNEMDYWNRLNSLNRARCENDVDFCLKQLQTAHDKAKIEPIKQAFQSSKGKERRQPNVDEIAAARIIQQILANPDCIHDDHVLINGQKLEQQFFRDLLAKCEMAVVGSLLNDTDIGNIDTLMRHEKDTEFHSTNPEAVPVKIGEYWINDQRINNSSGNITQKKHDLIFLMQNDAWYFSRVNAIAQNRDKGSTFKEVLITTLMTPLTSKALVDTSQAKPPTRLFRGLNLSEEFTKGLIDQANAMIANTTERLFTDHSPEAFKQIKLNDLSKMSGRTNASTTTEIKLVKETWDSNVIFEMLDPDGLLHSKQVGRHGEGTESEFSVYLPEDVALVPVKVTLDGKTQKGENRYVFTFVAVKSPDFTPRHESGYAVEPFLRMQAAKLAEVKSSIEKAQRAPDLETIFNLQNEVEAVQYSHLSTGYKNFLKNTVGPVLENSLSGLMESDTDTLSKALAAFPSDTQWSAFNFEEARQAKRQMDAIKQMVGNKVVLDALTQCQDALEKQNIAGALDALKKIPSEKEMGTIRRELREQIQSARQELESLQRAVVTPVVTDEKKVRERYDALIENTSKKITELETGKLPNLDAVKKGISNLSNLKQEVTVLRNEKIRMHVGTDKVDFSDVEKLEQQIQVIDTKLADAYLLEVTKQISALDNTKPKNQTELKTKIAAFLDRTTDIEMLRNERIKKHGSSKDPLDLSDLDKLSGSLQRINQSLVSDLITTIRVSINQMEAKTFHEQEKEIQQNFELLAKLEKTLDKSKTSEKLREDIPKLNDLLVAKQKAYPQMVQMQLKSEVFVTQLREVCQANHDDLDKTRNARLRELDRLDREAGITRMVGNLIWGLTNKVGLTTDERLDIRTKQQSLARFKNELFNDKIDTDQLISNLARKRPSELQEGLGISTDNAMELHLLLTELAGKTTSPDELEERMKAIDDISTKIGREPEHLKFVMVEEDESNKKTIGF rab33b-ub-1 3291102 N/A 3291107 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3291108 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false q6rcr0_legpn-ub-1 3291103 N/A 3291109 unspecified role unspecified role unspecified role unspecified role Q6RCR0:p.[Glu865Ala;Glu867Ala] E865A/E867A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3291107 unspecified role unspecified role unspecified role unspecified role GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false ecolx Escherichia coli direct interaction direct interaction false false false rab33b-ub-2 3291104 N/A 3291107 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3291108 unspecified role unspecified role unspecified role unspecified role Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false q6rcr0_legpn-ub-2 3291105 N/A 3291109 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3291107 unspecified role unspecified role unspecified role unspecified role GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false arg42ala R42A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false rab33b-ub-3 3291106 N/A 3291107 unspecified role unspecified role unspecified role unspecified role arg42ala R42A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3291108 unspecified role unspecified role unspecified role unspecified role Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false