DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ lin-2016-13 in vitro In vitro protease assay protease assay autoradiography autoradiography Lin Z., Luo X., Yu H. DIP original experiment: DIP-268762X Nature 2016 IMEx Structural basis of cohesin cleavage by separase. Lin Z., Luo X., Yu H. Only protein-protein interactions imex curation lin-2016-14 in vitro In vitro protease assay protease assay autoradiography autoradiography Lin Z., Luo X., Yu H. DIP original experiment: DIP-268761X Nature 2016 IMEx Structural basis of cohesin cleavage by separase. Lin Z., Luo X., Yu H. Only protein-protein interactions imex curation lin-2016-15 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Lin Z., Luo X., Yu H. DIP original experiment: DIP-268763X Nature 2016 IMEx Structural basis of cohesin cleavage by separase. Lin Z., Luo X., Yu H. Only protein-protein interactions imex curation g0scb6_chatd CTHT_0056640 protein protein chatd Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) MFWSGALLSSTGPLAKAWLSANQERKVSKVQIIQHNLQDSVEAIISPNEAPLALRLSGQLLLGVVRIYSRKARYLLDDCNEALMKIKMAFRSTGTHDVPTSMHVQNKESLILPDKITPYDNLELLPPPSADFLASQLEDVTATPISSRKTTLRPNLRDINLQEDYNNSQFLNDNSQDEDELGMANMDDLGLELDFGMDLDDRPSKLMDKSIEVGRDAPAARSVEDDLLSELDAGPNKDRLAREQSLGLDLDFGDGVQIADNDGDIQMRDDDLQFNVGDQSAMPEPIGADMSRARISESPLSDIDEDFAREVEEEYSRQMNNTDMYEPGDDSAATVQAPQRQKKKKLLQPDEQTMLSNAQIKELQSKRDSILKPQTFVAHDPYIVGLMDLHKNGGFVNNILFEGRSENWAPELKGLLSLGAIRPNVLKRKRDSGVADLGSEDEQGAHKSPRLELPEDETVLNLERSMVGNQSIAADGTVLSIPGQQDESTVVHGAEDDHHHSAASPGPAFEDTTMPLVHPADSGPVSVSTKHAVHILRDLFGEEAATNAEKRRKTHVVFQDLLPEGTTSKADATKMFFECLVLATKDAIKVEQQEGTLGGPIRIRGKRGLWGAWAEREAGGEMAREQVRDQAERVVAASQEVSVSA g0shm3_chatd Separase CTHT_0070540 protein protein chatd Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) MDSLQAQADAVRSAVTSVTTCSPATSSLLKTLLLDINNSATINSDSDSRPSSKTSNSRSRASTNTRAPSRRGKCDSPTPTSLSPREKAALATHVVNAALKALAEAAKTASPAPAPANSEPLSPARQQKGQEDSDLVKTVTKNNAAAIRRSSSAPMSPLQPRSLNRQTTPPAPTGSRPPSRGSNHSTNNNNNNNSSSSSSSSSSSSSSSSSSSSNSNNNSNSNSTNVNLLATVECARVALAALRQLPSTGKISPPELQLESGMSALIGRLITLGLHEQAIKELRILRKKLDTSSDKKTSSKSTEAKTTTTTTAAQVFTEILDFGSVAATGAKLLLIIQTQLHALRILATTTFTSKKPSTIAAILPLLRLDSHTSPLNLLLTASKEKGADTAKIARHTETLSQVLLALTPSVSSKDDTLALEPRLSIPPLAALELQALALEARIEWWRLAGHKGDAEKDIMIPLSRCLAAFARRSQDRSREAWGVCVRAFERVYKGVKNLGMSVAKGGRSALAGVLQSLVTVAKEGGLITEGVTWAGRLVEVVGAEKEGVKFCSLQAQVLGLKLKAPERFWGAEEDAELKKVMECIMGPLRGDTAELDELLGCVCGLRKGAMQVLLAAMRDNNNSGSEEAEYKPSPETRQLLESFVLQCPRFCLRWLGKPPGSRGNTKDYLRYDQRRQLLTQYLPHVLDSSLLLIKTLLDHGTPAWEQIDAILSDCSTLLEYTPLPDSSSPSSYYVKISHFHYLHFISLRQRSSNPQDVGPLRALRKAVDVVKHRSKPEKSAAKLTLKLERLADLCRSLGRIDEALSALQALRSSLVEDGVLRKIARRLQTESPVVVWNRDEGALPLGRCLVGIGRLETVWVDWTVELEEEQERGAVLEWWVRVGVLGRGEEVGLGHAVVERLLGVYIPTKYPVRRLRVLLLVLCAVLGDGKTAGEVLALAREASRVASGDDLGEDKGLASFLPHLKTLCASLEAAVDEYRDIRAVEQCLTSWRGIVSSCEDKNTLEQSIDDVPGLLEHLQAVADFHRMKGRETILISVLELMADISRLAEGPRAEDVIVHGSTFGLQLTNLGRAAKAEEVFRKMGEYLASLQPQDSSDNAAVGEGAVNYHLAMAEHFVALGQIRQAEEHLALAHNAASEEGAVQKYGKVERRRLVARACYLYSVVALERGDSHLALVYARESVRNLFSEWARLEAKMSSSSSSSSSPTETRQEQQHQDDPDAMDIDAAPPALESTRTPKRDAQHTAAGPEFWRLFCSLHRNISWLSAVYAHLGLFNETMYYAEQALRMANLIGSEFYSAQAAAWIASVAWRAADVQKAGEMLRQVMAGLLEKDGGCEYARAVLACRVSETALAQGDLESARRLLEWAEGVVRGIAMGGQKEVVTVEVAEEYEGRKAPIRATRTRKATTRAQSTTRKGAMRSAKGKSASTTTTPSNSRPGTPEVKEEDALLAKLKAYILVQKASMMLRQRDWAAAQQVLSEVPAPAVQSKSSSFLPARHLAMAACLLGMSMDQMAHDPVFSVIQDSTISFPALSGSGSVLLAGKGSPVKAAASPVVTSPRKPDLPTTTKTTTTTTETDPRVYVEHLKSAHDYLLEAHAVAALSGDASLVHRISGMLQNVGLILTATSTKIRPAAVPSAQTAYSVELARNLTWRRERKALLQEKKGLVNGNGTEMTSVQFPPPPNLAASAPRRSSLGFTLDLHRIQRDYIDLVPKHWHVISLSLSDGGHDLCITRLQAGQAPFVLRLPLERASSRDSSVDETDVFDFHTGRAELLEIIKEINRTCHDSRDMAAKGEREKWWAEREALDQRLKELLMNIEHVWLGGFRGVFSQHGRRPELLEKFRAMFEGVLDKHLPSRRQVGRGKKGKGVAGQTKVVLDGNVLELFIGLGDATKSGADFDEELTDLLYFVVDILQFHGERNAYDEIDFDSMVVETMDALMAYHAEANAAPESDSHAHTILVLDKQLHVFPWESLPCLQGLAVSRIPSLACLRKLLLDRRRSSSQIQGEDSEEEDPRSAGHHAPLSGGTYILNPSSDLLSTQKTFESLFSTHLHSPNSWTRIISRPPTEPEFLSALTHSPILLYFGHGSGAQYIRSRNIRHLDHCRATVLLMGCSSAALTAKGEFEPSGPVWNYMLAGAPAVVGTLWDVTDRDIDRFAGGVLEGWGVLPEGCMGEKNGKKKAGRNGLSLVQAVAKARDRCRFRYVTAAAAVVYGIPVYVDVDGKSKD g0s794_chatd CTHT_0027880 protein protein chatd Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) MLAAHHDQENLYTHQAGASKPQLQAKTPGARFPKTPLKVPLNDENTHHGFGGKNVLKAKGNNENILTIGKGGHVLGKGGKQALVTPAGPRTVRAPLGNKTTNAKARATQQTPAGKVILRDPQKTALKPTTAARPKLAAPKVEASKLEVHVDKDPLQVEEVEYAPPKPKEMPYESDVFPEGVLTYEGLKPGRLFKGYYQYYFNPIGEDGKTLQERDLEEQQKHCFERGEENILKDMEELDWTIGDLPESKEVLKKRTKRTPLSAASRPSAKGLLFSKSKPSTSAAPASTLPVRERPTAIDALRV g0scb6_chatd-g0shm3_chatd-1 3290793 N/A 3290796 enzyme target enzyme target substrate substrate unspecified role unspecified role 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false G0SCB6:p.Ile211Ala I211A mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3290797 enzyme enzyme unspecified role unspecified role Region 1501-2223 Region 1501-2223 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm protein cleavage protein cleavage false false false g0scb6_chatd-g0shm3_chatd-2 3290794 N/A 3290796 enzyme target enzyme target substrate substrate unspecified role unspecified role 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro N/A 3290797 enzyme enzyme unspecified role unspecified role Region 1501-2223 Region 1501-2223 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false G0SHM3:p.Cys2110Ser C2110S mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false G0SHM3:p.Trp1797Ala W1797A mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false G0SHM3:p.Trp1798Ala W1798A mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false G0SHM3:p.Arg1801Glu R1801E mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false G0SHM3:p.Phe2198Ala F2198A mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false G0SHM3:p.Tyr2200Ala Y2200A mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false G0SHM3:p.Met2108Ala M2108A mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false G0SHM3:p.Ser2112Ala S2112A mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false G0SHM3:p.Leu1985Ala L1985A mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false G0SHM3:p.Asp1698Lys D1698K mutation increasing rate mutation increasing interaction rate mutation increasing rate substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false G0SHM3:p.Asp1960Lys D1960K mutation increasing rate mutation increasing interaction rate mutation increasing rate substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false G0SHM3:p.Glu2120Lys E2120K mutation increasing rate mutation increasing interaction rate mutation increasing rate substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false G0SHM3:p.Phe2121Ala F2121A mutation increasing rate mutation increasing interaction rate mutation increasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false G0SHM3:p.His2083Ala H2083A mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false G0SHM3:p.Leu2034Ala L2034A mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false G0SHM3:p.Thr2037Ala T2037A mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false G0SHM3:p.Asp2151Ala D2151A mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false G0SHM3:p.Val2145Ala V2145A mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false G0SHM3:p.Thr2146Ala T2146A mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false G0SHM3:p.Asp2147Ala D2147A mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false G0SHM3:p.Arg2148Glu R2148E mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false G0SHM3:p.Asp2149Ala D2149A mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false G0SHM3:p.Arg2152Glu R2152E mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false G0SHM3:p.Cys1782Asp C1782D mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false G0SHM3:p.Asp2151Arg D2151R mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm protein cleavage protein cleavage false false false g0shm3_chatd-g0s794_chatd-1 3290795 N/A 3290798 unspecified role unspecified role prey prey Region 1-277 Region 1-277 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3290797 unspecified role unspecified role bait bait Region 315-2223 Region 315-2223 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Strep-tag Strep-tag strep tag strep tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 1-1500 Region 1-1500 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction false false false