DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ shi-2016-1 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Shi Y., Chen X., Elsasser S., Stocks BB., Tian G., Lee BH., Shi Y., Zhang N., de Poot SA., Tuebing F., Sun S., Vannoy J., Tarasov SG., Engen JR., Finley D., Walters KJ. DIP original experiment: DIP-269063X Science (New York, N.Y.) 2016 IMEx Rpn1 provides adjacent receptor sites for substrate binding and deubiquitination by the proteasome. Shi Y., Chen X., Elsasser S., Stocks BB., Tian G., Lee BH., Shi Y., Zhang N., de Poot SA., Tuebing F., Sun S., Vannoy J., Tarasov SG., Engen JR., Finley D., Walters KJ. Only protein-protein interactions imex curation shi-2016-2 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Shi Y., Chen X., Elsasser S., Stocks BB., Tian G., Lee BH., Shi Y., Zhang N., de Poot SA., Tuebing F., Sun S., Vannoy J., Tarasov SG., Engen JR., Finley D., Walters KJ. DIP original experiment: DIP-269067X Science (New York, N.Y.) 2016 IMEx Rpn1 provides adjacent receptor sites for substrate binding and deubiquitination by the proteasome. Shi Y., Chen X., Elsasser S., Stocks BB., Tian G., Lee BH., Shi Y., Zhang N., de Poot SA., Tuebing F., Sun S., Vannoy J., Tarasov SG., Engen JR., Finley D., Walters KJ. Only protein-protein interactions imex curation shi-2016-3 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Shi Y., Chen X., Elsasser S., Stocks BB., Tian G., Lee BH., Shi Y., Zhang N., de Poot SA., Tuebing F., Sun S., Vannoy J., Tarasov SG., Engen JR., Finley D., Walters KJ. DIP original experiment: DIP-269070X Science (New York, N.Y.) 2016 IMEx Rpn1 provides adjacent receptor sites for substrate binding and deubiquitination by the proteasome. Shi Y., Chen X., Elsasser S., Stocks BB., Tian G., Lee BH., Shi Y., Zhang N., de Poot SA., Tuebing F., Sun S., Vannoy J., Tarasov SG., Engen JR., Finley D., Walters KJ. Only protein-protein interactions imex curation shi-2016-4 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Shi Y., Chen X., Elsasser S., Stocks BB., Tian G., Lee BH., Shi Y., Zhang N., de Poot SA., Tuebing F., Sun S., Vannoy J., Tarasov SG., Engen JR., Finley D., Walters KJ. DIP original experiment: DIP-269073X Science (New York, N.Y.) 2016 IMEx Rpn1 provides adjacent receptor sites for substrate binding and deubiquitination by the proteasome. Shi Y., Chen X., Elsasser S., Stocks BB., Tian G., Lee BH., Shi Y., Zhang N., de Poot SA., Tuebing F., Sun S., Vannoy J., Tarasov SG., Engen JR., Finley D., Walters KJ. Only protein-protein interactions imex curation shi-2016-5 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Shi Y., Chen X., Elsasser S., Stocks BB., Tian G., Lee BH., Shi Y., Zhang N., de Poot SA., Tuebing F., Sun S., Vannoy J., Tarasov SG., Engen JR., Finley D., Walters KJ. DIP original experiment: DIP-269064X Science (New York, N.Y.) 2016 IMEx Rpn1 provides adjacent receptor sites for substrate binding and deubiquitination by the proteasome. Shi Y., Chen X., Elsasser S., Stocks BB., Tian G., Lee BH., Shi Y., Zhang N., de Poot SA., Tuebing F., Sun S., Vannoy J., Tarasov SG., Engen JR., Finley D., Walters KJ. Only protein-protein interactions imex curation shi-2016-6 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Shi Y., Chen X., Elsasser S., Stocks BB., Tian G., Lee BH., Shi Y., Zhang N., de Poot SA., Tuebing F., Sun S., Vannoy J., Tarasov SG., Engen JR., Finley D., Walters KJ. DIP original experiment: DIP-269068X Science (New York, N.Y.) 2016 IMEx Rpn1 provides adjacent receptor sites for substrate binding and deubiquitination by the proteasome. Shi Y., Chen X., Elsasser S., Stocks BB., Tian G., Lee BH., Shi Y., Zhang N., de Poot SA., Tuebing F., Sun S., Vannoy J., Tarasov SG., Engen JR., Finley D., Walters KJ. Only protein-protein interactions imex curation shi-2016-7 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Shi Y., Chen X., Elsasser S., Stocks BB., Tian G., Lee BH., Shi Y., Zhang N., de Poot SA., Tuebing F., Sun S., Vannoy J., Tarasov SG., Engen JR., Finley D., Walters KJ. DIP original experiment: DIP-269074X Science (New York, N.Y.) 2016 IMEx Rpn1 provides adjacent receptor sites for substrate binding and deubiquitination by the proteasome. Shi Y., Chen X., Elsasser S., Stocks BB., Tian G., Lee BH., Shi Y., Zhang N., de Poot SA., Tuebing F., Sun S., Vannoy J., Tarasov SG., Engen JR., Finley D., Walters KJ. Only protein-protein interactions imex curation shi-2016-8 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Shi Y., Chen X., Elsasser S., Stocks BB., Tian G., Lee BH., Shi Y., Zhang N., de Poot SA., Tuebing F., Sun S., Vannoy J., Tarasov SG., Engen JR., Finley D., Walters KJ. DIP original experiment: DIP-269077X Science (New York, N.Y.) 2016 IMEx Rpn1 provides adjacent receptor sites for substrate binding and deubiquitination by the proteasome. Shi Y., Chen X., Elsasser S., Stocks BB., Tian G., Lee BH., Shi Y., Zhang N., de Poot SA., Tuebing F., Sun S., Vannoy J., Tarasov SG., Engen JR., Finley D., Walters KJ. Only protein-protein interactions imex curation shi-2016-9 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Shi Y., Chen X., Elsasser S., Stocks BB., Tian G., Lee BH., Shi Y., Zhang N., de Poot SA., Tuebing F., Sun S., Vannoy J., Tarasov SG., Engen JR., Finley D., Walters KJ. DIP original experiment: DIP-269069X Science (New York, N.Y.) 2016 IMEx Rpn1 provides adjacent receptor sites for substrate binding and deubiquitination by the proteasome. Shi Y., Chen X., Elsasser S., Stocks BB., Tian G., Lee BH., Shi Y., Zhang N., de Poot SA., Tuebing F., Sun S., Vannoy J., Tarasov SG., Engen JR., Finley D., Walters KJ. Only protein-protein interactions imex curation shi-2016-10 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Shi Y., Chen X., Elsasser S., Stocks BB., Tian G., Lee BH., Shi Y., Zhang N., de Poot SA., Tuebing F., Sun S., Vannoy J., Tarasov SG., Engen JR., Finley D., Walters KJ. DIP original experiment: DIP-269065X Science (New York, N.Y.) 2016 IMEx Rpn1 provides adjacent receptor sites for substrate binding and deubiquitination by the proteasome. Shi Y., Chen X., Elsasser S., Stocks BB., Tian G., Lee BH., Shi Y., Zhang N., de Poot SA., Tuebing F., Sun S., Vannoy J., Tarasov SG., Engen JR., Finley D., Walters KJ. Only protein-protein interactions imex curation shi-2016-11 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Shi Y., Chen X., Elsasser S., Stocks BB., Tian G., Lee BH., Shi Y., Zhang N., de Poot SA., Tuebing F., Sun S., Vannoy J., Tarasov SG., Engen JR., Finley D., Walters KJ. DIP original experiment: DIP-269075X Science (New York, N.Y.) 2016 IMEx Rpn1 provides adjacent receptor sites for substrate binding and deubiquitination by the proteasome. Shi Y., Chen X., Elsasser S., Stocks BB., Tian G., Lee BH., Shi Y., Zhang N., de Poot SA., Tuebing F., Sun S., Vannoy J., Tarasov SG., Engen JR., Finley D., Walters KJ. Only protein-protein interactions imex curation shi-2016-12 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Shi Y., Chen X., Elsasser S., Stocks BB., Tian G., Lee BH., Shi Y., Zhang N., de Poot SA., Tuebing F., Sun S., Vannoy J., Tarasov SG., Engen JR., Finley D., Walters KJ. DIP original experiment: DIP-269071X Science (New York, N.Y.) 2016 IMEx Rpn1 provides adjacent receptor sites for substrate binding and deubiquitination by the proteasome. Shi Y., Chen X., Elsasser S., Stocks BB., Tian G., Lee BH., Shi Y., Zhang N., de Poot SA., Tuebing F., Sun S., Vannoy J., Tarasov SG., Engen JR., Finley D., Walters KJ. Only protein-protein interactions imex curation shi-2016-13 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Shi Y., Chen X., Elsasser S., Stocks BB., Tian G., Lee BH., Shi Y., Zhang N., de Poot SA., Tuebing F., Sun S., Vannoy J., Tarasov SG., Engen JR., Finley D., Walters KJ. DIP original experiment: DIP-269066X Science (New York, N.Y.) 2016 IMEx Rpn1 provides adjacent receptor sites for substrate binding and deubiquitination by the proteasome. Shi Y., Chen X., Elsasser S., Stocks BB., Tian G., Lee BH., Shi Y., Zhang N., de Poot SA., Tuebing F., Sun S., Vannoy J., Tarasov SG., Engen JR., Finley D., Walters KJ. Only protein-protein interactions imex curation shi-2016-14 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Shi Y., Chen X., Elsasser S., Stocks BB., Tian G., Lee BH., Shi Y., Zhang N., de Poot SA., Tuebing F., Sun S., Vannoy J., Tarasov SG., Engen JR., Finley D., Walters KJ. DIP original experiment: DIP-269076X Science (New York, N.Y.) 2016 IMEx Rpn1 provides adjacent receptor sites for substrate binding and deubiquitination by the proteasome. Shi Y., Chen X., Elsasser S., Stocks BB., Tian G., Lee BH., Shi Y., Zhang N., de Poot SA., Tuebing F., Sun S., Vannoy J., Tarasov SG., Engen JR., Finley D., Walters KJ. Only protein-protein interactions imex curation shi-2016-15 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Shi Y., Chen X., Elsasser S., Stocks BB., Tian G., Lee BH., Shi Y., Zhang N., de Poot SA., Tuebing F., Sun S., Vannoy J., Tarasov SG., Engen JR., Finley D., Walters KJ. DIP original experiment: DIP-269072X Science (New York, N.Y.) 2016 IMEx Rpn1 provides adjacent receptor sites for substrate binding and deubiquitination by the proteasome. Shi Y., Chen X., Elsasser S., Stocks BB., Tian G., Lee BH., Shi Y., Zhang N., de Poot SA., Tuebing F., Sun S., Vannoy J., Tarasov SG., Engen JR., Finley D., Walters KJ. Only protein-protein interactions imex curation ubiq_yeast Ubiquitin UBI1 RPL40A YIL148W UBI2 RPL40B YKR094C UBI3 RPS31 YLR167W L9470.14 UBI4 SCD2 YLL039C protein protein yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG obsolete protein rpn13_yeast 26S proteasome regulatory subunit RPN13 RPN13 DAQ1 Proteasome non-ATPase subunit 13 YLR421C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSMSSTVIKFRAGVCEYNEDSRLCTPIPVQGEIEIKPNEEEELGFWDFEWRPTEKPVGRELDPISLILIPGETMWVPIKSSKSGRIFALVFSSNERYFFWLQEKNSGNLPLNELSAKDKEIYNKMIGVLNNSSESDEEESNDEKQKAQDVDVSMQD 752E8A12DC3799D4 rpn10_yeast 26S proteasome regulatory subunit RPN10 RPN10 MCB1 SUN1 YHR200W protein protein yeast Saccharomyces cerevisiae Baker's yeast MVLEATVLVIDNSEYSRNGDFPRTRFEAQIDSVEFIFQAKRNSNPENTVGLISGAGANPRVLSTFTAEFGKILAGLHDTQIEGKLHMATALQIAQLTLKHRQNKVQHQRIVAFVCSPISDSRDELIRLAKTLKKNNVAVDIINFGEIEQNTELLDEFIAAVNNPQEETSHLLTVTPGPRLLYENIASSPIILEEGSSGMGAFGGSGGDSDANGTFMDFGVDPSMDPELAMALRLSMEEEQQRQERLRQQQQQQDQPEQSEQPEQHQDK 8A05198B0CD7C0C3 sic1_yeast Protein SIC1 SIC1 SDB25 YLR079W L9449.8 CDK inhibitor p40 protein protein yeast Saccharomyces cerevisiae Baker's yeast MTPSTPPRSRGTRYLAQPSGNTSSSALMQGQKTPQKPSQNLVPVTPSTTKSFKNAPLLAPPNSNMGMTSPFNGLTSPQRSPFPKSSVKRTLFQFESHDNGTVREEQEPLGRVNRILFPTQQNVDIDAAEEEEEGEVLLPPSRPTSARQLHLSLERDEFDQTHRKKIIKDVPGTPSDKVITFELAKNWNNNSPKNDARSQESEDEEDIIINPVRVGKNPFASDELVTQEIRNERKRAMLRENPDIEDVITYVNKKGEVVEKRRLTDEEKRRFKPKALFQSRDQEH 7913636340678F78 ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG psmd2_human 26S proteasome non-ATPase regulatory subunit 2 PSMD2 TRAP2 26S proteasome regulatory subunit RPN1 26S proteasome regulatory subunit S2 26S proteasome subunit p97 Tumor necrosis factor type 1 receptor-associated protein 2 Protein 55.11 protein protein human Homo sapiens Human MEEGGRDKAPVQPQQSPAAAPGGTDEKPSGKERRDAGDKDKEQELSEEDKQLQDELEMLVERLGEKDTSLYRPALEELRRQIRSSTTSMTSVPKPLKFLRPHYGKLKEIYENMAPGENKRFAADIISVLAMTMSGERECLKYRLVGSQEELASWGHEYVRHLAGEVAKEWQELDDAEKVQREPLLTLVKEIVPYNMAHNAEHEACDLLMEIEQVDMLEKDIDENAYAKVCLYLTSCVNYVPEPENSALLRCALGVFRKFSRFPEALRLALMLNDMELVEDIFTSCKDVVVQKQMAFMLGRHGVFLELSEDVEEYEDLTEIMSNVQLNSNFLALARELDIMEPKVPDDIYKTHLENNRFGGSGSQVDSARMNLASSFVNGFVNAAFGQDKLLTDDGNKWLYKNKDHGMLSAAASLGMILLWDVDGGLTQIDKYLYSSEDYIKSGALLACGIVNSGVRNECDPALALLSDYVLHNSNTMRLGSIFGLGLAYAGSNREDVLTLLLPVMGDSKSSMEVAGVTALACGMIAVGSCNGDVTSTILQTIMEKSETELKDTYARWLPLGLGLNHLGKGEAIEAILAALEVVSEPFRSFANTLVDVCAYAGSGNVLKVQQLLHICSEHFDSKEKEEDKDKKEKKDKDKKEAPADMGAHQGVAVLGIALIAMGEEIGAEMALRTFGHLLRYGEPTLRRAVPLALALISVSNPRLNILDTLSKFSHDADPEVSYNSIFAMGMVGSGTNNARLAAMLRQLAQYHAKDPNNLFMVRLAQGLTHLGKGTLTLCPYHSDRQLMSQVAVAGLLTVLVSFLDVRNIILGKSHYVLYGLVAAMQPRMLVTFDEELRPLPVSVRVGQAVDVVGQAGKPKTITGFQTHTTPVLLAHGERAELATEEFLPVTPILEGFVILRKNPNYDL rad23_yeast UV excision repair protein RAD23 SYGP-ORF29 YEL037C RAD23 protein protein yeast Saccharomyces cerevisiae Baker's yeast MVSLTFKNFKKEKVPLDLEPSNTILETKTKLAQSISCEESQIKLIYSGKVLQDSKTVSECGLKDGDQVVFMVSQKKSTKTKVTEPPIAPESATTPGRENSTEASPSTDASAAPAATAPEGSQPQEEQTATTERTESASTPGFVVGTERNETIERIMEMGYQREEVERALRAAFNNPDRAVEYLLMGIPENLRQPEPQQQTAAAAEQPSTAATTAEQPAEDDLFAQAAQGGNASSGALGTTGGATDAAQGGPPGSIGLTVEDLLSLRQVVSGNPEALAPLLENISARYPQLREHIMANPEVFVSMLLEAVGDNMQDVMEGADDMVEGEDIEVTGEAAAAGLGQGEGEGSFQVDYTPEDDQAISRLCELGFERDLVIQVYFACDKNEEAAANILFSDHAD B3F0436DAB60B833 rpn1_yeast 26S proteasome regulatory subunit RPN1 RPN1 HRD2 NAS1 RPD1 Proteasome non-ATPase subunit 1 HMG-CoA reductase degradation protein 2 YHR027C protein protein yeast Saccharomyces cerevisiae Baker's yeast MVDESDKKQQTIDEQSQISPEKQTPNKKDKKKEEEEQLSEEDAKLKTDLELLVERLKEDDSSLYEASLNALKESIKNSTSSMTAVPKPLKFLRPTYPDLCSIYDKWTDPNLKSSLADVLSILAMTYSENGKHDSLRYRLLSDVSDFEGWGHEYIRHLALEIGEVYNDQVEKDAEDETSSDGSKSDGSAATSGFEFSKEDTLRLCLDIVPYFLKHNGEEDAVDLLLEIESIDKLPQFVDENTFQRVCQYMVACVPLLPPPEDVAFLKTAYSIYLSQNELTDAIALAVRLGEEDMIRSVFDATSDPVMHKQLAYILAAQKTSFEYEGVQDIIGNGKLSEHFLYLAKELNLTGPKVPEDIYKSHLDNSKSVFSSAGLDSAQQNLASSFVNGFLNLGYCNDKLIVDNDNWVYKTKGDGMTSAVASIGSIYQWNLDGLQQLDKYLYVDEPEVKAGALLGIGISASGVHDGEVEPALLLLQDYVTNPDTKISSAAILGLGIAFAGSKNDEVLGLLLPIAASTDLPIETAAMASLALAHVFVGTCNGDITTSIMDNFLERTAIELKTDWVRFLALALGILYMGQGEQVDDVLETISAIEHPMTSAIEVLVGSCAYTGTGDVLLIQDLLHRLTPKNVKGEEDADEEETAEGQTNSISDFLGEQVNEPTKNEEAEIEVDEMEVDAEGEEVEVKAEITEKKNGESLEGEEIKSEEKKGKSSDKDATTDGKNDDEEEEKEAGIVDELAYAVLGIALIALGEDIGKEMSLRHFGHLMHYGNEHIRRMVPLAMGIVSVSDPQMKVFDTLTRFSHDADLEVSMNSIFAMGLCGAGTNNARLAQLLRQLASYYSREQDALFITRLAQGLLHLGKGTMTMDVFNDAHVLNKVTLASILTTAVGLVSPSFMLKHHQLFYMLNAGIRPKFILALNDEGEPIKVNVRVGQAVETVGQAGRPKKITGWITQSTPVLLNHGERAELETDEYISYTSHIEGVVILKKNPDYREEE B77954F4A998BA7C dsk2_yeast Ubiquitin domain-containing protein DSK2 DSK2 SHE4 YMR276W YM8021.02 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSLNIHIKSGQDKWEVNVAPESTVLQFKEAINKANGIPVANQRLIYSGKILKDDQTVESYHIQDGHSVHLVKSQPKPQTASAAGANNATATGAAAGTGATPNMSSGQSAGFNPLADLTSARYAGYLNMPSADMFGPDGGALNNDSNNQDELLRMMENPIFQSQMNEMLSNPQMLDFMIQSNPQLQAMGPQARQMLQSPMFRQMLTNPDMIRQSMQFARMMDPNAGMGSAGGAASAFPAPGGDAPEEGSNTNTTSSSNTGNNAGTNAGTNAGANTAANPFASLLNPALNPFANAGNAASTGMPAFDPALLASMFQPPVQASQAEDTRPPEERYEHQLRQLNDMGFFDFDRNVAALRRSGGSVQGALDSLLNGDV 3F8691BB95E46569 rpn13-yub-1 3290058 N/A 3290073 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3290074 unspecified role unspecified role unspecified role unspecified role O13563:p.[Glu41Lys;Glu42Lys;Leu43Ala;Phe45Ala;Ser93Asp] E41K/E42K/L43A/F45A/S93D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false rpn10-sic1-1 3290059 N/A 3290075 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3290076 unspecified role unspecified role prey prey Ub Ub ubiquitinylated lysine ubiquitinylated lysine undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Prerequisite-PTM T7-tag T7-tag t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false psmd2-hub-1 3290060 N/A 3290077 unspecified role unspecified role prey prey K48Ub K48Ub ubiquitinylated lysine ubiquitinylated lysine certain certain sequence position certain certain certain sequence position certain false K6Ub K6Ub ubiquitinylated lysine ubiquitinylated lysine certain certain sequence position certain certain certain sequence position certain false K11Ub K11Ub ubiquitinylated lysine ubiquitinylated lysine certain certain sequence position certain certain certain sequence position certain false K27Ub K27Ub ubiquitinylated lysine ubiquitinylated lysine certain certain sequence position certain certain certain sequence position certain false K29Ub K29Ub ubiquitinylated lysine ubiquitinylated lysine certain certain sequence position certain certain certain sequence position certain false K33Ub K33Ub ubiquitinylated lysine ubiquitinylated lysine certain certain sequence position certain certain certain sequence position certain false K63Ub K63Ub ubiquitinylated lysine ubiquitinylated lysine certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3290078 unspecified role unspecified role bait bait Region 404-617 Region 404-617 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false rpn1-rad23-3 3290061 N/A 3290079 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli N/A 3290080 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false Deletion of region 1-77 decreased the interaction rate/strength. rpn1-yub-1 3290062 N/A 3290080 unspecified role unspecified role unspecified role unspecified role Region 482-612 Region 482-612 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3290073 unspecified role unspecified role unspecified role unspecified role K48Ub K48Ub ubiquitinylated lysine ubiquitinylated lysine certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false rpn13-sic1-1 3290063 N/A 3290074 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3290076 unspecified role unspecified role prey prey Ub Ub ubiquitinylated lysine ubiquitinylated lysine undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Prerequisite-PTM T7-tag T7-tag t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false rpn1-rad23-4 3290064 N/A 3290079 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli N/A 3290080 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false P38764:p.[Asp541Ala;Asp548Arg;Glu552Arg] D541A/D548R/E552R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false dsk2-rpn1-1 3290065 N/A 3290081 unspecified role unspecified role unspecified role unspecified role Region 1-76 Region 1-76 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3290080 unspecified role unspecified role unspecified role unspecified role Region 412-625 Region 412-625 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false rpn1-yub-2 3290066 N/A 3290080 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3290073 unspecified role unspecified role prey prey K48Ub K48Ub ubiquitinylated lysine ubiquitinylated lysine certain certain sequence position certain certain certain sequence position certain false K6Ub K6Ub ubiquitinylated lysine ubiquitinylated lysine certain certain sequence position certain certain certain sequence position certain false K11Ub K11Ub ubiquitinylated lysine ubiquitinylated lysine certain certain sequence position certain certain certain sequence position certain false K27Ub K27Ub ubiquitinylated lysine ubiquitinylated lysine certain certain sequence position certain certain certain sequence position certain false K29Ub K29Ub ubiquitinylated lysine ubiquitinylated lysine certain certain sequence position certain certain certain sequence position certain false K33Ub K33Ub ubiquitinylated lysine ubiquitinylated lysine certain certain sequence position certain certain certain sequence position certain false K63Ub K63Ub ubiquitinylated lysine ubiquitinylated lysine certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false rpn1-yub-3 3290067 N/A 3290080 unspecified role unspecified role unspecified role unspecified role Region 482-612 Region 482-612 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3290073 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false rad23-rpn1-4 3290068 N/A 3290079 unspecified role unspecified role unspecified role unspecified role Region 1-77 Region 1-77 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3290080 unspecified role unspecified role unspecified role unspecified role Region 412-625 Region 412-625 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false rpn1-yub-4 3290069 N/A 3290080 unspecified role unspecified role unspecified role unspecified role Region 412-625 Region 412-625 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P38764:p.[Asp541Ala;Asp548Arg;Glu552Arg] D541A/D548R/E552R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3290073 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false rpn1-sic1-1 3290070 N/A 3290076 unspecified role unspecified role prey prey Ub Ub ubiquitinylated lysine ubiquitinylated lysine undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Prerequisite-PTM T7-tag T7-tag t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3290080 unspecified role unspecified role bait bait Region 376-635 Region 376-635 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false rad23-rpn1-5 3290071 N/A 3290079 unspecified role unspecified role unspecified role unspecified role Region 1-77 Region 1-77 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3290080 unspecified role unspecified role unspecified role unspecified role Region 412-625 Region 412-625 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P38764:p.[Asp541Ala;Asp548Arg;Glu552Arg] D541A/D548R/E552R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false rpn1-yub-5 3290072 N/A 3290080 unspecified role unspecified role bait bait Region 412-625 Region 412-625 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P38764:p.[Asp541Ala;Asp548Arg;Glu552Arg] D541A/D548R/E552R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P38764:p.[Ala514Gly;Asp517Ala;Asp541Ala;Asp548Arg;Glu552Arg] A514G/D517A/D541A/D548R/E552R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3290073 unspecified role unspecified role prey prey K48Ub K48Ub ubiquitinylated lysine ubiquitinylated lysine certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false