DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ jaeger-2016-1 ecolx Escherichia coli pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Jaeger AM., Pemble CW., Sistonen L., Thiele DJ. DIP original experiment: DIP-268800X Nature structural & molecular biology 2016 IMEx Structures of HSF2 reveal mechanisms for differential regulation of human heat-shock factors. Jaeger AM., Pemble CW., Sistonen L., Thiele DJ. Only protein-protein interactions imex curation jaeger-2016-2 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Jaeger AM., Pemble CW., Sistonen L., Thiele DJ. DIP original experiment: DIP-268802X Nature structural & molecular biology 2016 IMEx Structures of HSF2 reveal mechanisms for differential regulation of human heat-shock factors. Jaeger AM., Pemble CW., Sistonen L., Thiele DJ. Only protein-protein interactions imex curation jaeger-2016-3 ecolx Escherichia coli pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Jaeger AM., Pemble CW., Sistonen L., Thiele DJ. DIP original experiment: DIP-268805X Nature structural & molecular biology 2016 IMEx Structures of HSF2 reveal mechanisms for differential regulation of human heat-shock factors. Jaeger AM., Pemble CW., Sistonen L., Thiele DJ. Only protein-protein interactions imex curation jaeger-2016-4 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Jaeger AM., Pemble CW., Sistonen L., Thiele DJ. DIP original experiment: DIP-268803X Nature structural & molecular biology 2016 IMEx Structures of HSF2 reveal mechanisms for differential regulation of human heat-shock factors. Jaeger AM., Pemble CW., Sistonen L., Thiele DJ. Only protein-protein interactions imex curation jaeger-2016-5 ecolx Escherichia coli pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Jaeger AM., Pemble CW., Sistonen L., Thiele DJ. DIP original experiment: DIP-268801X Nature structural & molecular biology 2016 IMEx Structures of HSF2 reveal mechanisms for differential regulation of human heat-shock factors. Jaeger AM., Pemble CW., Sistonen L., Thiele DJ. Only protein-protein interactions imex curation jaeger-2016-6 ecolx Escherichia coli crosslink cross-linking study crosslink predetermined predetermined participant predetermined Jaeger AM., Pemble CW., Sistonen L., Thiele DJ. DIP original experiment: DIP-268807X Nature structural & molecular biology 2016 IMEx Structures of HSF2 reveal mechanisms for differential regulation of human heat-shock factors. Jaeger AM., Pemble CW., Sistonen L., Thiele DJ. Only protein-protein interactions imex curation jaeger-2016-7 mouse Mus musculus Mouse anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Jaeger AM., Pemble CW., Sistonen L., Thiele DJ. DIP original experiment: DIP-268804X Nature structural & molecular biology 2016 IMEx Structures of HSF2 reveal mechanisms for differential regulation of human heat-shock factors. Jaeger AM., Pemble CW., Sistonen L., Thiele DJ. Only protein-protein interactions imex curation jaeger-2016-8 ecolx Escherichia coli pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Jaeger AM., Pemble CW., Sistonen L., Thiele DJ. DIP original experiment: DIP-268806X Nature structural & molecular biology 2016 IMEx Structures of HSF2 reveal mechanisms for differential regulation of human heat-shock factors. Jaeger AM., Pemble CW., Sistonen L., Thiele DJ. Only protein-protein interactions imex curation hsf2_human Heat shock factor protein 2 HSTF2 Heat shock transcription factor 2 HSF2 protein protein human Homo sapiens Human MKQSSNVPAFLSKLWTLVEETHTNEFITWSQNGQSFLVLDEQRFAKEILPKYFKHNNMASFVRQLNMYGFRKVVHIDSGIVKQERDGPVEFQHPYFKQGQDDLLENIKRKVSSSKPEENKIRQEDLTKIISSAQKVQIKQETIESRLSELKSENESLWKEVSELRAKHAQQQQVIRKIVQFIVTLVQNNQLVSLKRKRPLLLNTNGAQKKNLFQHIVKEPTDNHHHKVPHSRTEGLKPRERISDDIIIYDVTDDNADEENIPVIPETNEDVISDPSNCSQYPDIVIVEDDNEDEYAPVIQSGEQNEPARESLSSGSDGSSPLMSSAVQLNGSSSLTSEDPVTMMDSILNDNINLLGKVELLDYLDSIDCSLEDFQAMLSGRQFSIDPDLLVDLFTSSVQMNPTDYINNTKSENKGLETTKNNVVQPVSEEGRKSKSKPDKQLIQYTAFPLLAFLDGNPASSVEQASTTASSEVLSSVDKPIEVDELLDSSLDPEPTQSKLVRLEPLTEAEASEATLFYLCELAPAPLDSDMPLLDS hsf1_human Heat shock factor protein 1 Heat shock transcription factor 1 HSF1 HSTF1 protein protein human Homo sapiens Human MDLPVGPGAAGPSNVPAFLTKLWTLVSDPDTDALICWSPSGNSFHVFDQGQFAKEVLPKYFKHNNMASFVRQLNMYGFRKVVHIEQGGLVKPERDDTEFQHPCFLRGQEQLLENIKRKVTSVSTLKSEDIKIRQDSVTKLLTDVQLMKGKQECMDSKLLAMKHENEALWREVASLRQKHAQQQKVVNKLIQFLISLVQSNRILGVKRKIPLMLNDSGSAHSMPKYSRQFSLEHVHGSGPYSAPSPAYSSSSLYAPDAVASSGPIISDITELAPASPMASPGGSIDERPLSSSPLVRVKEEPPSPPQSPRVEEASPGRPSSVDTLLSPTALIDSILRESEPAPASVTALTDARGHTDTEGRPPSPPPTSTPEKCLSVACLDKNELSDHLDAMDSNLDNLQTMLSSHGFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPRPPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAEDPTISLLTGSEPPKAKDPTVS hsf1-hsf2-1 3287449 N/A 3287457 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 119-192 Region 119-192 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3287458 unspecified role unspecified role bait bait StrepII-tag StrepII-tag strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false hsf1-hsf2-2 3287450 N/A 3287457 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3287458 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false hsf1-hsf2-3 3287451 N/A 3287457 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3287458 unspecified role unspecified role bait bait StrepII-tag StrepII-tag strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 130-203 Region 130-203 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false hsf1-1 3287452 N/A 3287458 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false hsf2-hsf1-1 3287453 N/A 3287457 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 119-192 Region 119-192 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3287458 unspecified role unspecified role prey prey StrepII-tag StrepII-tag strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false hsf1-hsf2-4 3287454 N/A 3287457 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3287458 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false hsf2-hsf1-2 3287455 N/A 3287457 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 119-192 Region 119-192 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false mouse Mus musculus Mouse N/A 3287458 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false mouse Mus musculus Mouse physical association physical association false false false hsf2-hsf1-3 3287456 N/A 3287457 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3287458 unspecified role unspecified role prey prey StrepII-tag StrepII-tag strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 130-203 Region 130-203 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false